| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75786 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54523-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48609-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22403-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73237 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61765-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95093-0.02ml | |||
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84323] - Staining of human cerebellum, fallopian tube, kidney and testis using Anti-STIM1 antibody NBP1-84323 (A) shows similar protein distribution across tissues to independent antibody NBP1-84322 (B).](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84323-img0027.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84323] - Staining of human cerebellum, fallopian tube, kidney and testis using Anti-STIM1 antibody NBP1-84323 (A) shows similar protein distribution across tissues to independent antibody NBP1-84322 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: STIM1 Antibody [NBP1-84323] - Staining of human cell line U-251 MG shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84323-img0019.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84323] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84323-img0030.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84323] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84323-img0025.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84323] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84323-img0028.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84323] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84323-img0029.jpg)
![Western Blot: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Western Blot Analysis of Human A431 cell lysate using Glut1 antibody.](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2475-Western-Blot-NBP2-75784-img0006.jpg "Western Blot: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Western Blot Analysis of Human A431 cell lysate using Glut1 antibody.")
![Protein Array: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Protein Array containing more than 19,000 full-length human proteins using Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2475-Protein-Array-NBP2-75784-img0005.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human tongue stained with GLUT-1 Mouse Monoclonal Antibody (GLUT1/2475).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2475-Immunohistochemistry-Paraffin-NBP2-75784-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Immunofluorescence staining of K562 cells using Glut1Mouse Monoclonal Antibody (GLUT1/2475) followed by goat anti-Mouse IgG-CF488 (green). Nuclei are stained with Reddot.](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2475-Immunocytochemistry-Immunofluorescence-NBP2-75784-img0007.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human bladder carcinoma stained with GLUT-1 Mouse Monoclonal Antibody (GLUT1/2475).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2475-Immunohistochemistry-Paraffin-NBP2-75784-img0002.jpg)
![Flow Cytometry: Glut1 Antibody (GLUT1/2475) [NBP2-75784] - Flow Cytometric analysis of K562 cells using Glut1 antibody followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2475-Flow-Cytometry-NBP2-75784-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: P2Y1/P2RY1 Antibody [NBP1-30741] - Dissociated rat hippocampal culture. Image courtesy of Janosch Heller, University College London.](http://images.novusbio.com/fullsize/P2Y1-P2RY1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30741-img0002.jpg "Immunocytochemistry/Immunofluorescence: P2Y1/P2RY1 Antibody [NBP1-30741] - Dissociated rat hippocampal culture. Image courtesy of Janosch Heller, University College London.")
![Western Blot: N-Cadherin Antibody (8C11) [NBP2-53321] - Western blot analysis of Heart tissue lysate using N-Cadherin antibody (8C11).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-8C11-Western-Blot-NBP2-53321-img0002.jpg "Western Blot: N-Cadherin Antibody (8C11) [NBP2-53321] - Western blot analysis of Heart tissue lysate using N-Cadherin antibody (8C11).")
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (8C11) [NBP2-53321] - Mouse heart stained with N-Cadherein antibody (8C11).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-8C11-Immunohistochemistry-Paraffin-NBP2-53321-img0001.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human placenta and testis tissues. Corresponding SLC2A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human placenta and testis tissues. Corresponding SLC2A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Glut1 Antibody [NBP2-48609] - Immunocytochemistry analysis of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48609-img0018.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human cerebral cortex shows strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0013.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Staining of human skin shows moderate membranous positivity in epidermal cells.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-NBP2-48609-img0019.jpg)
![Immunohistochemistry: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of mouse brain shows moderate positivity in endothelial cells in the cerebral cortex.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0012.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0014.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of human testis shows no membranous positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0016.jpg)
![Western Blot: ABCC9 Antibody (S319A-14) [NBP2-22403] - Western Blot analysis of Rat Brain Membrane showing detection of ~120 kDa ABCC9 protein using Mouse Anti-ABCC9 Monoclonal Antibody, Clone S319A-14 (NBP2-22403). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-ABCC9 Monoclonal Antibody (NBP2-22403) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~120 kDa.](http://images.novusbio.com/fullsize/ABCC9-Antibody-S319A-14-Western-Blot-NBP2-22403-img0003.jpg "Western Blot: ABCC9 Antibody (S319A-14) [NBP2-22403] - Western Blot analysis of Rat Brain Membrane showing detection of ~120 kDa ABCC9 protein using Mouse Anti-ABCC9 Monoclonal Antibody, Clone S319A-14 (NBP2-22403). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-ABCC9 Monoclonal Antibody (NBP2-22403) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~120 kDa.")
![Immunocytochemistry/Immunofluorescence: ABCC9 Antibody (S319A-14) [NBP2-22403] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ABCC9 Monoclonal Antibody, Clone S319A-14 (NBP2-22403). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-ABCC9 Monoclonal Antibody (NBP2-22403) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ABCC9 Antibody (D) Composite.](http://images.novusbio.com/fullsize/ABCC9-Antibody-S319A-14-Immunocytochemistry-Immunofluorescence-NBP2-22403-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ABCC9 Antibody (S319A-14) [NBP2-22403] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SUR2A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/ABCC9-Antibody-S319A-14-Immunocytochemistry-Immunofluorescence-NBP2-22403-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ABCC9 Antibody (S319A-14) [NBP2-22403] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ABCC9 Monoclonal Antibody, Clone S319A-14 (NBP2-22403). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ABCC9 Monoclonal Antibody (NBP2-22403) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ABCC9 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/ABCC9-Antibody-S319A-14-Immunocytochemistry-Immunofluorescence-NBP2-22403-img0005.jpg)
![Western Blot: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cadherin.](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Western-Blot-NBP2-46200-img0011.jpg "Western Blot: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cadherin.")
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0010.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0001.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0002.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0003.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0004.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0005.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0006.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0007.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0008.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0009.jpg)
![Western Blot: P2Y8 Antibody (1G5A2) [NBP2-61765] - Analysis using P2RY8 mAb against human P2RY8 (AA: extra mix) recombinant protein. (Expected MW is 6.6 kDa)](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A2-Western-Blot-NBP2-61765-img0005.jpg "Western Blot: P2Y8 Antibody (1G5A2) [NBP2-61765] - Analysis using P2RY8 mAb against human P2RY8 (AA: extra mix) recombinant protein. (Expected MW is 6.6 kDa)")
![Immunocytochemistry/Immunofluorescence: P2Y8 Antibody (1G5A2) [NBP2-61765] - Analysis of Hela cells using P2RY8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A2-Immunocytochemistry-Immunofluorescence-NBP2-61765-img0003.jpg)
![Immunohistochemistry-Paraffin: P2Y8 Antibody (1G5A2) [NBP2-61765] - Analysis of cervical cancer tissues using P2RY8 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A2-Immunohistochemistry-NBP2-61765-img0004.jpg)
![Western Blot: P2Y8 Antibody (1G5A2) [NBP2-61765] - Analysis using P2RY8 mAb against HEK293 (1) and P2RY8 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A2-Western-Blot-NBP2-61765-img0006.jpg)
![ELISA: P2Y8 Antibody (1G5A2) [NBP2-61765] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A2-ELISA-NBP2-61765-img0001.jpg)
![ELISA: P2Y8 Antibody (1G5A2) [NBP2-61765] - Analysis of Hela cells using P2RY8 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A2-ELISA-NBP2-61765-img0002.jpg)
![Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - A surface stain was performed on A549 cells with E-Cadherin Antibody (67A4) NBP1-42793AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-67A4-Flow-Cytometry-NBP1-42793-img0003.jpg "Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - A surface stain was performed on A549 cells with E-Cadherin Antibody (67A4) NBP1-42793AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - Surface staining of HT-29 / SP2 cells with anti-CD324 (67A4) PE.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-67A4-Flow-Cytometry-NBP1-42793-img0001.jpg)
![Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - Surface staining of HT-29 / SP2 cells with anti-CD324 (67A4) APC.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-67A4-Flow-Cytometry-NBP1-42793-img0002.jpg)
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414262648.jpg "Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32385, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414333665.jpg)
![Immunocytochemistry/ Immunofluorescence: Glut1 Antibody (SA0377) [NBP3-32385] - Immunocytochemistry analysis of HeLa cells labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202410334092.jpg)
![Flow Cytometry: Glut1 Antibody (SA0377) [NBP3-32385] - Flow cytometric analysis of Jurkat cells labeling Glut1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32385, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (blue). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202416372147.jpg)
![Western Blot: Glut1 Antibody (SA0377) [NBP3-32385] - Western blot analysis of Glut1 on different lysates with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/50,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: HT-29 cell lysate (no heat) Lane 3: HepG2 cell lysate (no heat) Lane 4: NIH/3T3 cell lysate (no heat) Lane 5: L-929 cell lysate (no heat) Lane 6: Mouse brain tissue lysate (no heat) Lane 7: Rat brain tissue lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: Lane 1-7 (left): 20 seconds; Lane 1-7 (right): 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-3920248453511.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Beta 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Western-Blot-NBP2-95093-img0004.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Beta 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded mouse stomach using Sodium Potassium ATPase Beta 1 .](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0003.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded human colon carcinoma using Sodium Potassium ATPase Beta 1 .](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0001.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded human gastric cancer using Sodium Potassium ATPase Beta 1 .](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0002.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Western blot analysis of various lysates using Sodium Potassium ATPase Beta 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-95093_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-52202516345269.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Immunofluorescence analysis of NIH/3T3 cells using Sodium Potassium ATPase Beta 1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95093_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-5220251638037.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Immunofluorescence analysis of C6 cells using Sodium Potassium ATPase Beta 1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95093_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-5220251628041.jpg)