| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05549-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59310-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76789-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-21805-0.1ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61671-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95255-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84588-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43888 | |||
| BSA Free | NBP2-34476-25ul | |||
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987] - Staining of Rat Retina lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Western-Blot-NBP1-51987-img0001.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987] - Staining of Rat Retina lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987] - Staining of Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP1-51987-img0003.jpg)
![Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Western-Blot-NBP3-05549-img0002.jpg "Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05549] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Ankyrin 3 Antibody, CPCA-ANK3, at a dilution of 1:2,000 in green, and costained with mouse mAb to RBFOX3/NeuN at a dilution of 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The Ankyrin 3 antibody stains the axonal initial segments, while the RBFOX3/NeuN antibody reveals perikarya and nuclei of neurons.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05549-img0001.jpg)
![Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/E-Cadherin-[p-Ser838--p-Ser840]-Antibody-Western-Blot-NBP3-12922-img0004.jpg "Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - E-Cadherin (phospho Ser838/840) antibody detects E-Cadherin (phospho Ser838/840) protein by western blot analysis. MCF-7 whole cell extract (30 ug) with the indicated treatments were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) at a dilution of 1:10000.](http://images.novusbio.com/fullsize/E-Cadherin-[p-Ser838--p-Ser840]-Antibody-Western-Blot-NBP3-12922-img0001.jpg)
![Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - E-Cadherin (phospho Ser838/840) antibody detects E-Cadherin (phospho Ser838/840) protein by western blot analysis. Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) diluted at 1:10000.](http://images.novusbio.com/fullsize/E-Cadherin-[p-Ser838--p-Ser840]-Antibody-Western-Blot-NBP3-12922-img0002.jpg)
![Immunoprecipitation: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - Immunoprecipitation of E-Cadherin (phospho Ser838/840) protein from MCF-7 whole cell extracts using 5 ug of E-Cadherin (phospho Ser838/840) antibody (NBP3-12922). Western blot analysis was performed using E-Cadherin (phospho Ser838/840) antibody (NBP3-12922). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/E-Cadherin-[p-Ser838--p-Ser840]-Antibody-Immunoprecipitation-NBP3-12922-img0003.jpg)
![Western Blot: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Western Blot analysis of Rat brain membrane lysate showing detection of Ankyrin 3 protein using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:1000.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-S106-20-Western-Blot-NBP2-59310-img0005.jpg "Western Blot: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Western Blot analysis of Rat brain membrane lysate showing detection of Ankyrin 3 protein using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin 3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-S106-20-Immunocytochemistry-Immunofluorescence-NBP2-59310-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin G Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-S106-20-Immunocytochemistry-Immunofluorescence-NBP2-59310-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ankyrin 3 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-S106-20-Immunocytochemistry-Immunofluorescence-NBP2-59310-img0007.jpg)
![Western Blot: STIM1 Antibody [NBP1-76789] - Mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/STIM1-Antibody-Western-Blot-NBP1-76789-img0001.jpg "Western Blot: STIM1 Antibody [NBP1-76789] - Mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-76789] - Human spleen tissue with Stromal interaction molecule 1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-76789-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: STIM1 Antibody - BSA Free [NBP1-76789] - Immunofluorescence of STIM1 in Hek293 cells with STIM1 antibody at 20 u/mL.Red: STIM1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76789_rabbit-polyclonal-stim1-antibody-2711202420285877.gif)
![Immunocytochemistry/ Immunofluorescence: STIM1 Antibody - BSA Free [NBP1-76789] - Immunofluorescence of STIM1 in mouse spleen tissue with STIM1 antibody at 20 u/mL.Green: STIM1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76789_rabbit-polyclonal-stim1-antibody-2711202420195326.gif)
![Immunohistochemistry: STIM1 Antibody - BSA Free [NBP1-76789] - Immunohistochemistry of STIM1 in mouse spleen tissue with STIM1 antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp1-76789_rabbit-polyclonal-stim1-antibody-2711202419541585.gif)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunofluorescence-NBP2-21805-img0002.jpg "Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.")
![Immunohistochemistry: N-Cadherin Antibody (GC-4) [NBP2-21805] - Frozen acetone fixed chicken cardiac sections stained with Monoclonal Anti-N-Cadherin antibody produced in Mouse clone GC-4: followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunohistochemistry-NBP2-21805-img0001.jpg)
![Western Blot: P2Y1/P2RY1 Antibody (4G5B7) [NBP2-61671] - Analysis using P2RY1 mAb against human P2RY1 (AA: extra mix) recombinant protein. (Expected MW is 13 kDa)](http://images.novusbio.com/fullsize/P2Y1-P2RY1-Antibody-4G5B7-Western-Blot-NBP2-61671-img0005.jpg "Western Blot: P2Y1/P2RY1 Antibody (4G5B7) [NBP2-61671] - Analysis using P2RY1 mAb against human P2RY1 (AA: extra mix) recombinant protein. (Expected MW is 13 kDa)")
![Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody (4G5B7) [NBP2-61671] - Analysis of rectum cancer tissues using P2RY1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/P2Y1-P2RY1-Antibody-4G5B7-Immunohistochemistry-NBP2-61671-img0004.jpg)
![Flow Cytometry: P2Y1/P2RY1 Antibody (4G5B7) [NBP2-61671] - Analysis of Hela cells using P2RY1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/P2Y1-P2RY1-Antibody-4G5B7-Flow-Cytometry-NBP2-61671-img0002.jpg)
![Western Blot: P2Y1/P2RY1 Antibody (4G5B7) [NBP2-61671] - Analysis using P2RY1 mAb against HEK293 (1) and P2RY1 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/P2Y1-P2RY1-Antibody-4G5B7-Western-Blot-NBP2-61671-img0006.jpg)
![Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody (4G5B7) [NBP2-61671] - Analysis of bladder cancer tissues using P2RY1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/P2Y1-P2RY1-Antibody-4G5B7-Immunohistochemistry-NBP2-61671-img0003.jpg)
![ELISA: P2Y1/P2RY1 Antibody (4G5B7) [NBP2-61671] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/P2Y1-P2RY1-Antibody-4G5B7-ELISA-NBP2-61671-img0001.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Western blot analysis of extracts of various cell lines, using Sodium Potassium ATPase Alpha 1 antibody (NBP2-95255) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Sodium Potassium ATPase Alpha 1 Antibody-Western Blot-NBP2-95255-img0004.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Western blot analysis of extracts of various cell lines, using Sodium Potassium ATPase Alpha 1 antibody (NBP2-95255) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Immunohistochemistry of paraffin-embedded mouse kidney using Sodium Potassium ATPase Alpha 1 Rabbit pAb (NBP2-95255) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95255-img0003.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Immunohistochemistry of paraffin-embedded human liver cancer using Sodium Potassium ATPase Alpha 1 Rabbit pAb (NBP2-95255) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95255-img0001.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Immunohistochemistry of paraffin-embedded human lung cancer using Sodium Potassium ATPase Alpha 1 Rabbit pAb (NBP2-95255) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95255-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody - Azide and BSA Free [Sodium Potassium ATPase Alpha 1] - Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using Sodium Potassium ATPase Alpha 1 Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp2-95255_rabbit-polyclonal-sodium-potassium-atpase-alpha-1-antibody-5220251640221.jpg)
![Western Blot: CD98 Antibody [H00006520-D01P] - Analysis of SLC3A2 expression in rat brain.](http://images.novusbio.com/fullsize/CD98-Antibody-Western-Blot-H00006520-D01P-img0009.jpg "Western Blot: CD98 Antibody [H00006520-D01P] - Analysis of SLC3A2 expression in rat brain.")
![Western Blot: CD98 Antibody [H00006520-D01P] - Analysis of SLC3A2 expression in A-431.](http://images.novusbio.com/fullsize/CD98-Antibody-Western-Blot-H00006520-D01P-img0006.jpg)
![Western Blot: CD98 Antibody [H00006520-D01P] - Analysis of SLC3A2 expression in mouse kidney.](http://images.novusbio.com/fullsize/CD98-Antibody-Western-Blot-H00006520-D01P-img0007.jpg)
![Western Blot: CD98 Antibody [H00006520-D01P] - Analysis of SLC3A2 expression in mouse brain.](http://images.novusbio.com/fullsize/CD98-Antibody-Western-Blot-H00006520-D01P-img0008.jpg)
![Western Blot: CD98 Antibody [H00006520-D01P] - Analysis of SLC3A2 expression in transfected 293T cell line by SLC3A2 polyclonal antibody.Lane 1: SLC3A2 transfected lysate(57.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CD98-Antibody-Western-Blot-H00006520-D01P-img0010.jpg)
![Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32290) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29820241435227.jpg "Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32290) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32290, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with 33258 (blue).](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29820241435238.jpg)
![Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunocytochemistry analysis of HT-29 (positive) and MDA-MB-231 (negative) cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29202410484863.jpg)
![Flow Cytometry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Flow cytometric analysis of HT-29 (positive, red) and MDA-MB-231 (negative, green) cells labeling E-Cadherin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32290, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HT-29 black, MDA-MB-231 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29202416453231.jpg)
![Western Blot: E-Cadherin Antibody (SY0287) [NBP3-32290] - Western blot analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-231 cell lysate (negative) Lane 3: HT-29 cell lysate Lane 4: HCT 116 cell lysate Lane 5: A431 cell lysate Lane 6: Caco-2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 80~120 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-3920248453539.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP1-84588] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP1-84588-img0005.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP1-84588] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.")
![Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP1-84588] - Staining of human colon shows moderate positivity in plasma membrane in glandular cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP1-84588-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP1-84588] - Staining of human fallopian tube shows moderate positivity in plasma membrane of glandular cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP1-84588-img0003.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP1-84588] - Staining of human prostate shows moderate positivity in plasma membrane in glandular cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP1-84588-img0004.jpg)
![Western Blot: E-Cadherin Antibody (CL1172) [NBP2-34476] - Lane 1: Marker [kDa,Lane 2: Human cell line MCF-7, Lane 3: Human cell line SK-BR-3, Lane 4: Human cell line HeLa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1172-Western-Blot-NBP2-34476-img0009.jpg "Western Blot: E-Cadherin Antibody (CL1172) [NBP2-34476] - Lane 1: Marker [kDa,Lane 2: Human cell line MCF-7, Lane 3: Human cell line SK-BR-3, Lane 4: Human cell line HeLa")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1172) [NBP2-34476] - Staining in human duodenum and cerebral cortex tissues. Corresponding CDH1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1172-Immunohistochemistry-Paraffin-NBP2-34476-img0014.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1172) [NBP2-34476] - Staining of human prostate shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1172-Immunohistochemistry-Paraffin-NBP2-34476-img0011.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1172) [NBP2-34476] - Staining of human colorectal cancer shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1172-Immunohistochemistry-Paraffin-NBP2-34476-img0012.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1172) [NBP2-34476] - Staining of human cerebral cortex shows no positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1172-Immunohistochemistry-Paraffin-NBP2-34476-img0013.jpg)