DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the astrocytes showing nuclear staining. Protocol available in COMET™ Panel Builder." alt="p53 was detected in immersion fixed paraffin-embedded sections of human Colon Tumor using Mouse Anti-Human p53 Monoclonal Antibody (Catalog # NBP2-29453) at 12ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the astrocytes showing nuclear staining. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33074 | |||
| IHC-Prediluted | NBP2-44976 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12872-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36573SS | |||
| Azide and BSA Free | NBP2-80951 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47483SS | |||
| Azide and BSA Free | NBP2-80972 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2381SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87842-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-189SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05203-0.025ml | |||
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0010.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - p53 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using a 1 ug/mL mouse anti-p53 monoclonal (NBP2-29453, Novus Biologicals) for 1 hour at room temperature followed by the appropriate HRP polymer reagent, anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0008.jpg)
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of p53 1) A431 2) MCF7 and 3) HEK293 lysate probed with p53 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0001.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - Formalin-paraffin human colon carcinoma stained with p53 Ab (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0003.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0004.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0005.jpg)
![Western Blot: PSD-95 Antibody (7E3) [NBP2-12872] - Western Blot analysis of Rat brain membrane lysate showing detection of PSD-95 protein using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:1000.](http://images.novusbio.com/fullsize/PSD-95-Antibody-7E3-Western-Blot-NBP2-12872-img0013.jpg "Western Blot: PSD-95 Antibody (7E3) [NBP2-12872] - Western Blot analysis of Rat brain membrane lysate showing detection of PSD-95 protein using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.](http://images.novusbio.com/fullsize/PSD-95-Antibody-7E3-Immunocytochemistry-Immunofluorescence-NBP2-12872-img0015.jpg)
![Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872] - Immunohistochemistry analysis using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining. Backskin obtained from transgenic mice. .](http://images.novusbio.com/fullsize/PSD-95-Antibody-7E3-Immunohistochemistry-NBP2-12872-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872] - Mouse brain cultured neurons. Image from verified customer review.](http://images.novusbio.com/fullsize/PSD-95-Antibody-7E3-Immunocytochemistry-Immunofluorescence-NBP2-12872-img0012.jpg)
![Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872] - Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/PSD-95-Antibody-7E3-Immunohistochemistry-NBP2-12872-img0010.jpg)
![Immunohistochemistry-Frozen: PSD-95 Antibody (7E3) [NBP2-12872] - Mouse brain section (hippocampus). Image from verified customer review.](http://images.novusbio.com/fullsize/PSD-95-Antibody-7E3-Immunohistochemistry-Frozen-NBP2-12872-img0011.jpg)
![Immunohistochemistry-Paraffin: Dopamine D1R/DRD1 Antibody [NBP2-16213] - U373 xenograft, using Dopamine Receptor D1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Dopamine-D1-R-DRD1-Antibody-Immunohistochemistry-Paraffin-NBP2-16213-img0003.jpg "Immunohistochemistry-Paraffin: Dopamine D1R/DRD1 Antibody [NBP2-16213] - U373 xenograft, using Dopamine Receptor D1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Dopamine Receptor D1 antibody [C2C3], C-term detects Dopamine Receptor D1 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [C2C3], C-term (NBP2-16213) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16213_rabbit-polyclonal-dopamine-d1r-drd1-antibody-immunocytochemistry-immunofluorescence-2611202518321913.jpg)
![Dopamine Receptor D1 antibody [C2C3], C-term detects Dopamine Receptor D1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebral cortex.Green: Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [C2C3], C-term (NBP2-16213) diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16213_rabbit-polyclonal-dopamine-d1r-drd1-antibody-immunohistochemistry-frozen-2611202518241621.jpg)
![Dopamine Receptor D1 antibody [C2C3], C-term detects Dopamine Receptor D1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [C2C3], C-term (NBP2-16213) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16213_rabbit-polyclonal-dopamine-d1r-drd1-antibody-immunocytochemistry-immunofluorescence-261120251834121.jpg)
![Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of SLC31A1/CTR1 in MCF7 and HeLa at 2 ug/ml.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Western-Blot-NBP2-36573-img0001.jpg "Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of SLC31A1/CTR1 in MCF7 and HeLa at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti SLC31A1/CTR1 (5E6.1B4) NBP2-36573 at a 1:50 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-36573-img0009.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a formalin fixed and paraffin embedded tissue section of human urinary bladder transitional cell carcinoma (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0002.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a FFPE tissue section of normal breast tissue from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The antibody generated a very strong cytoplasmic staining in the myo-epithelial cells in the tested section.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0008.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a FFPE tissue section of normal skin from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The antibody developed a strong cytoplasmic staining in the outer layer of stratum spinosum and the cells of stratum granulosum/ granular layer of skin epidermis. The endothelial cells in the dermis of the section also showed positivity for SLC31A1/CTR1.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0006.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Aanalysis of a FFPE tissue section of human small bowel malignant stromal tumor (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The cancer cells developed moderate to strong positivity for CTR1/SLC31A1 protein.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0007.jpg)
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [FITC] [NBP2-27229] - TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE this antibody and 1 ug of either isotype control (Left), or TLR4-FITC (right). PPI negative, CD14+ cells were gated for analysis. Image using the FITC form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0008.jpg "Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [FITC] [NBP2-27229] - TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE this antibody and 1 ug of either isotype control (Left), or TLR4-FITC (right). PPI negative, CD14+ cells were gated for analysis. Image using the FITC form of this antibody.")
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Analysis of Alexa Fluor (R) 488 conjugate of NBP2-27231. Cell surface 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram represents human PBMC alone; green represents mouse isotype control. FITC-conjugated goat anti-mouse secondary](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0002.jpg)
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Cell surface analysis of 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram represents human PBMC alone; green represents mouse isotype control . FITC-conjugated goat anti-mouse secondary antibody was used for this experiment.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0001.jpg)
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0003.jpg)
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 488] [NBP2-27231AF488] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0004.jpg)
![Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor (R) 647] [NBP2-27228] - Cell surface analysis of CD86 in human monocytes using 0.1 ug of NBP2-00308 conjugated to Alexa Fluor 647. The shaded histogram represents human monocytes alone; green represents mouse isotype control ; red represents CD86 this antibody. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cell-Surface-NBP2-27231-img0005.jpg)
![Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 700] [NBP2-27231AF700] - A surface stain was performed on hPBMCs with CCR1 (53504) antibody FAB145N and a matched isotype control NBP2-27231AF700. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488. Image using the Alexa Fluor 700 form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cell-Surface-NBP2-27231-img0006.jpg)
![Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Allophycocyanin] [NBP2-27231APC] - A surface stain was performed on human peripheral blood monocytes with EMMPRIN/CD147 (MEM-M6/2) antibody NB500-561APC (blue) and a matched isotype control NBP2-27231APC (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cell-Surface-NBP2-27231-img0007.jpg)
![Western Blot: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of THP-1 cell lysate.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-18-Western-Blot-NB100-74510-img0013.jpg "Western Blot: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of THP-1 cell lysate.")
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of Presenilin 1 using Presenilin 1 Monoclonal antibody (APS 18) shows staining in A2058 melanoma cells. Presenilin 1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 at a dilution of 1:20-1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-18-Immunocytochemistry-Immunofluorescence-NB100-74510-img0022.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 18) [NB100-74510] - Normal biopsies of deparaffinized Human liver tissue.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-18-Immunohistochemistry-Paraffin-NB100-74510-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 18) [NB100-74510] - Staining of PS1 in mouse fibroblasts.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-18-Immunocytochemistry-Immunofluorescence-NB100-74510-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of Presenilin 1 using Presenilin 1 Monoclonal antibody (APS 18) shows staining in MCF-7 cells. Presenilin 1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 at a dilution of 1:20-1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-18-Immunocytochemistry-Immunofluorescence-NB100-74510-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of Presenilin 1 using Presenilin 1 Monoclonal antibody (APS 18) shows staining in HeLa cells. Presenilin 1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 at a dilution of 1:20-1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-18-Immunocytochemistry-Immunofluorescence-NB100-74510-img0021.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 18) [NB100-74510] - Normal biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-18-Immunohistochemistry-Paraffin-NB100-74510-img0023.jpg)
![Western Blot: Synaptophysin Antibody (7H12) [NBP1-47483] - Analysis using Synaptophysin mouse mAb against rat brain tissue lysate.](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-7H12-Western-Blot-NBP1-47483-img0004.jpg "Western Blot: Synaptophysin Antibody (7H12) [NBP1-47483] - Analysis using Synaptophysin mouse mAb against rat brain tissue lysate.")
![Immunohistochemistry-Paraffin: Synaptophysin Antibody (7H12) [NBP1-47483] - Human pancreas (FFPE) stained with Synaptophysin antibody. pH 9 HIER antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-7H12-Immunohistochemistry-Paraffin-NBP1-47483-img0005.jpg)
![Immunohistochemistry-Paraffin: Synaptophysin Antibody (7H12) [NBP1-47483] - Analysis of paraffin-embedded human small intestine, myenteric plexus tissues using anti-Synaptophysin mouse mAb.](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-7H12-Immunohistochemistry-Paraffin-NBP1-47483-img0003.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Mouse whole brain lysate showing specific immunolabeling of the ~46 kDa gamma-2-subunit of GABAa -R.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Western-Blot-NB300-190-img0002.jpg "Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Mouse whole brain lysate showing specific immunolabeling of the ~46 kDa gamma-2-subunit of GABAa -R.")
![Immunohistochemistry: GABA-A R gamma 2 Antibody [NB300-190] - Immunostaining of mouse retina showing specific labeling of the GABAa gamma-2 subunit in green, calbindin in red and DNA in blue. Photo courtesy of Dr. Arlene Hirano, UCLA.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-190-img0001.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Pattern of YFP gamma 2 expression in retinas of Thy1-YFP gamma 2 transgenic mice.(A) Maximum intensity projections (MIP) of confocal image stacks (2 µm total thickness) of vertical slices of Thy1-YFP gamma 2 retinas at postnatal day (P)12 & P21. Diffuse fluorescence expression in cell bodies within the ganglion cell layer (GCL) at both ages, & punctate expression throughout both OFF & ON layers of the inner plexiform layer (IPL) are apparent. There was no expression in the outer plexiform layer (OPL) or the outer nuclear layer (ONL) at either age. (B) Images of MIPs (∼20 µm thick) at various depths of a P21 flat mount Thy1-YFP gamma 2 retina. Inserts are 3× magnification of the images. (C) Western blot of whole brain lysates from Thy1-YFP gamma 2 (Tg) & wildtype (WT) mice. YFP gamma 2 (arrowhead) was detected with anti-GABAAR gamma 2 (upper) & anti-GFP (lower). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-31020241620510.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212325.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212373.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-31020241621817.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212319.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416205172.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416205153.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212384.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212327.jpg)
![Western Blot: VEGF Antibody [NB100-2381] - Detection of VEGFA doublet in CSF-IR/VEGFA chimera transfected lysate using.](http://images.novusbio.com/fullsize/VEGFA-Antibody-Western-Blot-NB100-2381-img0005.jpg "Western Blot: VEGF Antibody [NB100-2381] - Detection of VEGFA doublet in CSF-IR/VEGFA chimera transfected lysate using.")
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding SYNJ1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding SYNJ1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol and centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87842-img0022.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0020.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human cerebellum shows moderate positivity in neuropil as well as in a subset of neurons.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0017.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human cerebral cortex shows positivity in neuropil as well as moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0018.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human rectum shows moderate cytoplasmic positivity in neurons in peripheral ganglia.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0019.jpg)
![Western Blot: metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in mouse brain lysate at 0.5 ug/ml.](http://images.novusbio.com/fullsize/metabotropic-Glutamate-Receptor-6-Antibody-Western-Blot-NB300-189-img0006.jpg "Western Blot: metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in mouse brain lysate at 0.5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: mGluR6 Antibody [NB300-189] - metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in trapezoid body in mouse brain using NB300-189 and anti-rabbit Cy3 conjugated secondary antibodies (red). DAPI was used to counterstain cell nuclei.](http://images.novusbio.com/fullsize/metabotropic-Glutamate-Receptor-6-Antibody-Immunocytochemistry-Immunofluorescence-NB300-189-img0005.jpg)
![Western Blot: MBP Antibody (7G7) [NBP1-05203] - Analysis of different tissue lysates using mouse mAb to MBP, NBP1-05203, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, [6] mouse spinal cord. Multiple bands at 14kDa, 17kDa, 18.5kDa to 21.5kDa are the alternate transcripts of MBP. Other bands are proteolytic fragments of the MBP protein.](http://images.novusbio.com/fullsize/MBP-Antibody-7G7-Western-Blot-NBP1-05203-img0002.jpg "Western Blot: MBP Antibody (7G7) [NBP1-05203] - Analysis of different tissue lysates using mouse mAb to MBP, NBP1-05203, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, [6] mouse spinal cord. Multiple bands at 14kDa, 17kDa, 18.5kDa to 21.5kDa are the alternate transcripts of MBP. Other bands are proteolytic fragments of the MBP protein.")
![Immunohistochemistry: MBP Antibody (7G7) [NBP1-05203] - Analysis of rat hippocampal section stained with mouse mAb to myelin basic protein (MBP), NBP1-05203, dilution 1:5,000 in green, and costained with rabbit pAb to neurofilament NF-H, dilution 1:2,000, in red. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons, while the NF-H antibody labels the axons themselves.](http://images.novusbio.com/fullsize/MBP-Antibody-7G7-Immunocytochemistry-Immunofluorescence-NBP1-05203-img0001.jpg)
![Myelination protein expression is downregulated in response to ER stress. The myelination proteins (A) MBP [t (4) = 3.056, p = 0.0378], (B) PLP [t (4) = 29.84, p < 0.0001], and (C) CNPase [t (4) = 4.797, p = 0.0093] were all significantly downregulated after 24-hour exposure to 10 μg/mL tunicamycin compared to control cells. *p < 0.05; **p < 0.01; ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39411401), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05203_mouse-monoclonal-mbp-antibody-7g7-western-blot-132202615324617.jpg)
