| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-28904SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33102 | |||
| IHC-Prediluted | NBP2-44724 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22399-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-43661-25ul | |||
| Azide and BSA Free | NBP3-44321 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05210-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-43662-25ul | |||
| Azide and BSA Free | NBP3-44322 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05209-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33062 | |||
| IHC-Prediluted | NBP2-48235 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24570-25ul | |||
| Azide and BSA Free | NBP3-43844 | |||
![Western Blot: NF-H Antibody [NB300-135] - Proof of viability of organotypic adult brain slices. Brain slices of adult 9 month old wildtype (WT) mice were dissected fresh or incubated for either 1 day or for 14 days to examine vitality of slices (A). Brain slices were subsequently analyzed by Western blot using antibodies against Neurofilament-200 kDa, total tau (Tau-5) and actin served as a control. Size markers are given as kDa on the right side. Note some degradation of neurofilament but stability of Tau-5. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnagi.2018.00113/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NF-H-Antibody-Western-Blot-NB300-135-img0004.jpg "Western Blot: NF-H Antibody [NB300-135] - Proof of viability of organotypic adult brain slices. Brain slices of adult 9 month old wildtype (WT) mice were dissected fresh or incubated for either 1 day or for 14 days to examine vitality of slices (A). Brain slices were subsequently analyzed by Western blot using antibodies against Neurofilament-200 kDa, total tau (Tau-5) and actin served as a control. Size markers are given as kDa on the right side. Note some degradation of neurofilament but stability of Tau-5. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnagi.2018.00113/full) licensed under a CC-BY license.")
![Immunohistochemistry: NF-H Antibody [NB300-135] - Mouse hippocampus section stained with rabbit pAb to NF-H, dilution 1:2,000 in red, and costained with mouse mAb to myelin basic protein (MBP), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunohistochemistry-NB300-135-img0002.jpg)
![Immunohistochemistry: NF-H Antibody [NB300-135] - Immunostainings of adult organotypic brain slices at the hippocampal level. Brain slices were prepared from WT or TG adult mice and incubated with okadaic acid (OA). Brain slices were stained for nuclear DAPI (blue, A,B) showing intact brain structures. Thiazine Red staining (C,D) shows red fluorescent plaques in the hippocampus (D) of TG mice, while only background is seen in the hippocampus (C) of WT mice. Staining of neurofilament fibers (G,H) shows good neuronal viability both in WT and TG mice. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnagi.2018.00113/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunohistochemistry-NB300-135-img0005.jpg)
![Western Blot: NF-H Antibody [NB300-135] - Analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract. The first lane is Coomassie Brilliant Blue stained and the second lane is probed with rabbit anti-Neurofilament Heavy antibody NB300-135. The NF-H corresponds to a weight of 200kDa.](http://images.novusbio.com/fullsize/200kDa-Neurofilament-Heavy-Antibody-Western-Blot-NB300-135-img0001.jpg)
![Western Blot: NF-H Antibody [NB300-135] - Different tissue lysates using rabbit pAb to NF-H, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse spinal cord lysate. Strong band at about 220 kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected with RPCA-NF-H antibody.](http://images.novusbio.com/fullsize/NF-H-Antibody-Western-Blot-NB300-135-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28904-img0009.jpg "Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. IHC localization of LGR5 in the ZG. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 um) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients and two from pheochromocytoma patients. Scale bar, 500 um (low power) and 100 um (high power). C, capsule. Image collected and cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0011.jpg)
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Analysis of human small intestine.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0005.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. A, Heat map of 37 genes (shown on the vertical axis), which are 5-fold or greater differentially expressed in ZG & ZF. Genes previously associated with the Wnt signaling pathway are highlighted in red. On the horizontal axis, each pair of ZG & ZF from the 20 adrenals are anonymized & numbered. Probe set & detailed microarray information are available from the National Center for Biotechnology Information Gene Expression Omnibus under accession number GSE64957. B, IHC localization of LGR5 & downstream Wnt signaling proteins in the ZG: from the canonical beta -catenin pathway, beta -catenin & LEF1, & from the noncanonical AP1/JUN pathway, c-JUN & p-c-JUN. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 μm) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Interestingly, beta -catenin staining was mostly membranous or cytoplasmic (rather than nuclear staining as highlighted by the arrows), indicating limited canonical Wnt activation. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients & two from pheochromocytoma patients. Scale bar, 500 μm (low power) & 100 μm (high power). C, capsule. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28904_rabbit-polyclonal-lgr5-gpr49-antibody-31020241618943.jpg)
![Western Blot: NF-H Antibody [NB300-217] - Analysis of spinal cord lysates from different species using chicken pAb to NF-H, NB300-217, dilution 1:20,000 in green: [1] protein standard (red), [2] rat, [3] mouse, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the phosphorylated from of NF-H. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H.](http://images.novusbio.com/fullsize/NF-H-Antibody-Western-Blot-NB300-217-img0007.jpg "Western Blot: NF-H Antibody [NB300-217] - Analysis of spinal cord lysates from different species using chicken pAb to NF-H, NB300-217, dilution 1:20,000 in green: [1] protein standard (red), [2] rat, [3] mouse, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the phosphorylated from of NF-H. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H.")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Imaging of Feline optic nerve at antibody dilution 1:5000. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunocytochemistry-Immunofluorescence-NB300-217-img0006.jpg)
![Immunohistochemistry Free-Floating: NF-H Antibody [NB300-217] - Analysis of a rat cerebellum section stained with NF-H antibody, dilution 1:5,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free floating sections were stained with above antibodies. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunohistochemistry-Free-Floating-NB300-217-img0009.jpg)
![Western Blot: NF-H Antibody [NB300-217] - Analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract. The first lane is Coomassie Brilliant Blue stained and the second lane is probed with chicken anti-Neurofilament Heavy antibody NB300-217.](http://images.novusbio.com/fullsize/NF-H-Antibody-Western-Blot-NB300-217-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Rat neurons stained with 200kDa Neurofilament Heavy Antibody NB300-217.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunocytochemistry-Immunofluorescence-NB300-217-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - SH-SY5Y cells stained with 200kDa Neurofilament Heavy Antibody NB300-217 (red) and Fibrillarin Antibody NB300-269 (green). Nuclear DNA is stained with Hoechst dye (blue).](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunocytochemistry-Immunofluorescence-NB300-217-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Mixed rat neuron/glial cultures stained with Neurofilament Light (NF-L) antibody NBP1-05217 [green] and Neurofilament Heavy (NF-H) antibody NB300-217 [red]. Blue is a DNA stain. NB300-217 binds primarily to the phosphorylated axonal forms of NF-H, in contrast to NBP1-05217 which stains both axonal and dendritic/perikaryal neurofilaments. The surrounding axonal profiles are orange due to staining of both the NF-H and NF-L antibodies.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunocytochemistry-Immunofluorescence-NB300-217-img0004.jpg)
![Immunohistochemistry-Frozen: NF-H Antibody [NB300-217] - Canine optic nerve, antibody specifically labelled the axons (Green). Blue is DAPI. Image taken with an epifluorescent microscope and was incubated at 1:10000 for 1hr at RT.. Image from verified customer review.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunohistochemistry-Frozen-NB300-217-img0008.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody [NB300-217] - NF-H immunoreactivity in a FFPE section of mouse trigeminal ganglion. Antibody was diluted 1 to 2000 and left on sections for 1h at room temperature. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-217_chicken-polyclonal-nf-h-antibody-immunohistochemistry-paraffin-204202311828.jpg)
![Immunohistochemistry-Frozen: Chicken Polyclonal NF-H Antibody [NB300-217] - Fixed frozen section of mouse optic nerve showing neurofilament heavy NB300-217 in green and S100B in red. NB300-217 was diluted 1 in 4000 and was left on tissue sections overnight at room temperature. Primary was detected with donkey anti chicken conjugated to Alexa 488. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-217_chicken-polyclonal-nf-h-antibody-immunohistochemistry-frozen-842024131739..png)
![Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of canine cecum showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-217_chicken-polyclonal-nf-h-antibody-immunohistochemistry-paraffin-2942024113042.png)
![Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of feline colon showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-217_chicken-polyclonal-nf-h-antibody-immunohistochemistry-paraffin-2942024113519.png)
![Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of equine jejunum showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-217_chicken-polyclonal-nf-h-antibody-immunohistochemistry-paraffin-2942024114043..png)
![Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of human colon showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-217_chicken-polyclonal-nf-h-antibody-immunohistochemistry-paraffin-2942024114432..png)
![Western Blot: NF-H Antibody (NF421) [NBP2-29629] - Western Blot Analysis of Human Brain Tissue Lysate using NF-H antibody (NF421).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Western-Blot-NBP2-29629-img0007.jpg "Western Blot: NF-H Antibody (NF421) [NBP2-29629] - Western Blot Analysis of Human Brain Tissue Lysate using NF-H antibody (NF421).")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody (NF421) [NBP2-29629] - Immunofluorescence staining of HEK293 cells using NF-H Antibody (NF421) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunocytochemistry-Immunofluorescence-NBP2-29629-img0005.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NF421) [NBP2-29629] - Formalin-fixed, paraffin-embedded Rat Cerebellum stained with NF-H Antibody (NF421).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunohistochemistry-Paraffin-NBP2-29629-img0006.jpg)
![Flow Cytometry: NF-H Antibody (NF421) [NBP2-29629] - Flow Cytometric Analysis of HEK293 cells using NF-H Antibody (NF421) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Flow-Cytometry-NBP2-29629-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NF421) [NBP2-29629] - Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (RT-91).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunohistochemistry-Paraffin-NBP2-29629-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NF421) [NBP2-29629] - Formalin-fixed, paraffin-embedded human cerebellum stained with neurofilament monoclonal Antibody (RT-97)](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunohistochemistry-Paraffin-NBP2-29629-img0003.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NF421) [NBP2-29629] - Formalin-fixed, paraffin-embedded human cerebellum stained with Neurofilament NF421 antibody (1:200), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunohistochemistry-Paraffin-NBP2-29629-img0001.jpg)
![Western Blot: GluR1 Antibody (S355-1) [NBP2-22399] - Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa GluR1 protein using Mouse Anti-GluR1 Monoclonal Antibody, Clone S355-1 (NBP2-22399). Load: 10 ug. Block: 5% milk + TBST. Primary Antibody: Mouse Anti-GluR1 Monoclonal Antibody (NBP2-22399) at 1:2000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.](http://images.novusbio.com/fullsize/GluR1-Antibody-S355-1-Western-Blot-NBP2-22399-img0004.jpg "Western Blot: GluR1 Antibody (S355-1) [NBP2-22399] - Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa GluR1 protein using Mouse Anti-GluR1 Monoclonal Antibody, Clone S355-1 (NBP2-22399). Load: 10 ug. Block: 5% milk + TBST. Primary Antibody: Mouse Anti-GluR1 Monoclonal Antibody (NBP2-22399) at 1:2000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.")
![Immunocytochemistry/Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluA1/GluR1 Monoclonal Antibody, Clone S355-1 (NBP2-22399). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluA1/GluR1 Monoclonal Antibody (NBP2-22399) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluA1/GluR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GluR1-Antibody-S355-1-Immunocytochemistry-Immunofluorescence-NBP2-22399-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X.](http://images.novusbio.com/fullsize/GluR1-Antibody-S355-1-Immunocytochemistry-Immunofluorescence-NBP2-22399-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody, Clone S355-1 (NBP2-22399). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody (NBP2-22399) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluA1/GluR1 Glutamate Receptor Antibody. (D) Composite.](http://images.novusbio.com/fullsize/GluR1-Antibody-S355-1-Immunocytochemistry-Immunofluorescence-NBP2-22399-img0006.jpg)
![Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Analysis of different tissue lysates using mouse mAb to NF-H, dilution 1:10000 (Green): [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Western-Blot-NBP1-05210-img0006.jpg "Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Analysis of different tissue lysates using mouse mAb to NF-H, dilution 1:10000 (Green): [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody (9B12) [NBP1-05210] - Mixed neuron/glial cultures stained with NBP1-05210 (red) and rabbit GFAP antibody NB300-141 (green). Axonal profiles are stained in red, while astrocytic cells are revealed in green. Nuclei are revealed with a fluorescent DNA stain (blue).](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Immunocytochemistry-Immunofluorescence-NBP1-05210-img0003.jpg)
![Immunohistochemistry Free-Floating: NF-H Antibody (9B12) [NBP1-05210] - Analysis of a rat brain coronal section of the third ventricle stained with mouse monoclonal antibody to phosphorylated NF-H, dilution 1:5,000 (Green). Hoechst staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibody. The antibody is a robust marker of the axons of neuronal cells.](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Immunohistochemistry-Free-Floating-NBP1-05210-img0005.jpg)
![Wallerian degeneration and mitochondrial degeneration in axon for rat’s distal nerve stump. a, Representative images of toluidine blue staining, immunohistochemistry (NF-H), electron microscopy, before (pre) and 1, 2, and 14 d after axotomy. Immunohistochemistry was obtained the same results for three individuals. Scale bar, * 10 μm, ** 2 μm. Arrows, myelinated axon; arrowheads, demyelinated Schwann cell. b, Quantification of the myelinated axon. c, d, Quantification of the G-ratio [c: cumulative G-ratio per individual, d: scatter plot, Spearman’s rank correlation coefficient and p values showing G-ratio of individual myelinated axons against axon diameter (n = 400)]. The histologic WD was observed significantly from 2 d after axotomy. In axons, before (pre) and 1 and 2 d after axotomy were evaluated, except for after 14 d with completely collapsed axon morphology (n = 4 rat per group). e, Representative images of mitochondrial findings, before (pre) and 1, 2, and 14 d after axotomy. Evaluation was performed in axons and Schwann cells separately. Scale bar, * 200 nm. f, Violin plot of mitochondrial diameter (nm; n = 200). Cumulative mitochondria diameter per individual are also shown (n = 4 rat per group). g, Quantitative analysis of atypical mitochondria (%; n = 4 rat per group). In axons, mitochondria were significantly degenerated from 1 d after axotomy, whereas in Schwann cells, mitochondria were not significantly degenerated until 14 d after axotomy. All histologic evaluations were performed 3 mm distal to the sectional end and corresponding uninjured nerve. Error bars indicate SD; *p < 0.05, ***p < 0.001, one-way ANOVA followed by the Tukey’s post hoc test (for comparison of axons and Schwann cells, respectively). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36894321), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05210_mouse-monoclonal-nf-h-antibody-9b12-immunocytochemistry-immunofluorescence-132202617595724.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Anaysis of Lgr5/GPR49 with Brain, hippocampus](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0015.jpg "Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Anaysis of Lgr5/GPR49 with Brain, hippocampus")
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of anti-GPR49 antibody with human, small intestine.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0011.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of anti-GPR49 antibody with human colon.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0012.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of Lgr5/GPR49 with brain, glioblastoma](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0013.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of Lgr5/GPR49 with Brain, Caudate](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0014.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NLS1236] - Immunohistochemistry of formalin-fixed, paraffin-embedded Human Brain, Cortex tissue](http://images.novusbio.com/fullsize/nls1236_rabbit-lgr5-gpr49-pab-bsa-free-10920241103115.jpg)
![Western Blot: NF-H Antibody (AH1) [NBP1-05209] - Analysis of the heavily phosphorylated axonal form of NF-H protein (pNF-H) in neural tissue lysates (20ug/lane) with affinity purified mouse monoclonal anti-pNF-H antibody (NBP1-05209) at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain [4] Adult rat spinal cord [5] Adult mouse whole brain [6] Adult mouse spinal cord. Rodent pNF-H protein appears as a single band of about 200kDa in adult rat and mouse lysates, but is not present in early development (Lane 3). Additional bands appearing on the blot (Lane 4) are most likely partially degraded products of pNF-H protein.](http://images.novusbio.com/fullsize/NF-H-Antibody-AH1-Western-Blot-NBP1-05209-img0005.jpg "Western Blot: NF-H Antibody (AH1) [NBP1-05209] - Analysis of the heavily phosphorylated axonal form of NF-H protein (pNF-H) in neural tissue lysates (20ug/lane) with affinity purified mouse monoclonal anti-pNF-H antibody (NBP1-05209) at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain [4] Adult rat spinal cord [5] Adult mouse whole brain [6] Adult mouse spinal cord. Rodent pNF-H protein appears as a single band of about 200kDa in adult rat and mouse lysates, but is not present in early development (Lane 3). Additional bands appearing on the blot (Lane 4) are most likely partially degraded products of pNF-H protein.")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody (AH1) [NBP1-05209] - Mixed neuron/glial cultures stained with NBP1-05209 (green) and also stained with rabbit polyclonal antibody to neurofilament NF-L NB300-131 (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NBP1-05209 binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom since they contain both NF-L and phosphorylated NF-H they appear golden in color. Blue shows the distribution of DNA.](http://images.novusbio.com/fullsize/NF-H-Antibody-AH1-Immunocytochemistry-Immunofluorescence-NBP1-05209-img0003.jpg)
![Immunohistochemistry Free-Floating: NF-H Antibody (AH1) [NBP1-05209] - Analysis of rat cerebellum section stained with mouse mAb to pNF-H, NBP1-05209, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-05209 antibody stains axons in the granular layer and white matter and prominent basket cell axons surrounding the large Purkinje neurons. The FOX3/NeuN antibody specifically labels nuclei of granular and other neurons, but does not stain Purkinje cells.](http://images.novusbio.com/fullsize/NF-H-Antibody-AH1-Immunocytochemistry-Immunofluorescence-NBP1-05209-img0004.jpg)
![Western Blot: NF-H Antibody (RT97) [NBP2-29435] - Western Blot Analysis of human brain tissue lysate using NF-H Antibody (RT97).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT97-Western-Blot-NBP2-29435-img0008.jpg "Western Blot: NF-H Antibody (RT97) [NBP2-29435] - Western Blot Analysis of human brain tissue lysate using NF-H Antibody (RT97).")
![Immunohistochemistry-Paraffin: NF-H Antibody (RT97) [NBP2-29435] - Formalin-fixed, paraffin-embedded Rat Cerebellum stained with NF-H Antibody (RT97).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT97-Immunohistochemistry-Paraffin-NBP2-29435-img0007.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (RT97) [NBP2-29435] - Formalin-fixed, paraffin-embedded human Cerebellum stained with NF-H Antibody (RT97).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT97-Immunohistochemistry-Paraffin-NBP2-29435-img0006.jpg)
