| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56326SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87781-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24669SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50036SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40773SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54439-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59668SS | |||
| Azide and BSA Free | NBP2-80958 | |||
![Western Blot: SOX2 Antibody [NBP2-24390] - Analysis of SOX2 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/SOX2-Antibody-Western-Blot-NBP2-24390-img0002.jpg "Western Blot: SOX2 Antibody [NBP2-24390] - Analysis of SOX2 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: SOX2 Antibody [NBP2-24390] - FFPE human skin stained with SOX2 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/SOX2-Antibody-Immunohistochemistry-Paraffin-NBP2-24390-img0003.jpg)
![Western Blot: MeCP2 Antibody [NB100-56326] - Analysis of MeCP2 in HeLa cell lysate using this antibody.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NB100-56326-img0002.jpg "Western Blot: MeCP2 Antibody [NB100-56326] - Analysis of MeCP2 in HeLa cell lysate using this antibody.")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB100-56326] - This antibody was tested in A431 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56326-img0003.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Mouse liver tissue using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0007.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Tissue section of mouse pancreas using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in majority of the cells from acini, ducts and Islets of Langerhans in the section.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0004.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Mouse liver tissue using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0006.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP2-42847-img0010.jpg "Western Blot: GAP-43 Antibody [NBP2-42847] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP2-42847] - GAP-43 antibody detects GAP-43 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAP-43 protein stained by GAP-43 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42847-img0011.jpg)
![Immunohistochemistry-Frozen: GAP-43 Antibody [NBP2-42847] - GAP-43 antibody detects GAP-43 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: GAP-43 protein stained by GAP-43 antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunohistochemistry-Frozen-NBP2-42847-img0007.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP2-42847-img0006.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP2-42847-img0008.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP2-42847-img0009.jpg)
![Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-42847] - Cal27 xenograft. GAP43 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunohistochemistry-Paraffin-NBP2-42847-img0002.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody (NBP2-42847) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-42847_rabbit-polyclonal-gap-43-antibody-91020242021945.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining in human cerebral cortex and pancreas tissues using anti-PLP1 antibody. Corresponding PLP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining in human cerebral cortex and pancreas tissues using anti-PLP1 antibody. Corresponding PLP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Border of a demyelinated lesion. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0013.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0010.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0011.jpg)
![Western Blot: MeCP2 Antibody [NB600-1101] - Analysis of HeLa cell extract.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NB600-1101-img0006.jpg "Western Blot: MeCP2 Antibody [NB600-1101] - Analysis of HeLa cell extract.")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB600-1101] - Analysis of Methyl CpG Binding Protein 2 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1101-img0004.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB600-1101] - Staining in the nucleus of rat brain tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB600-1101-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB600-1101] - Analysis of Methyl CpG Binding Protein 2 (green) showing staining in the nucleus of C6 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1101-img0003.jpg)
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Human brain tissue using anti-NeuN antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Immunohistochemistry-Paraffin-NBP2-67314-img0008.jpg "Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Human brain tissue using anti-NeuN antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Immunohistochemistry-Paraffin-NBP2-67314-img0004.jpg)
![Western Blot: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Analysis of RBFOX/NeuN on different lysates with Rabbit anti-RBFOX/NeuN antibody at 1/500 dilution. Lane 1: Rat cerebellum tissue lysate Lane 2: Mouse cerebellum tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 34 kDa Observed band size: 45/50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Western-Blot-NBP2-67314-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Analysis of SHG-44 cells labeling RBFOX3/NeuN with Rabbit anti-RBFOX3/NeuN antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NeuN antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Immunocytochemistry-Immunofluorescence-NBP2-67314-img0011.jpg)
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Immunofluorescence analysis of paraffin-embedded Rat cerebellum tissue labeling NeuN ( washed with PBS. And then probed with the primary antibodies NeuN ( Green) at 1/50 dilution and GFAP (Red) at 1/500 dilution overnight at 4 , washed with PBS. iFluor(TM) 488 conjugate-Goat anti-Rabbit IgG and iFluor(TM) 594 conjugate-Goat anti-Mouse IgG were used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Immunohistochemistry-Paraffin-NBP2-67314-img0016.jpg)
![Flow Cytometry: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Analysis of SH-SY-5Y cells with NeuN antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Flow-Cytometry-NBP2-67314-img0001.jpg)
![Western Blot: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Analysis of NeuN on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human brain tissue lysateLane 2: rat brain tissue lysate Lane 2: mouse brain tissue lysate](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Western-Blot-NBP2-67314-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Immunocytochemistry-Immunofluorescence-NBP2-67314-img0002.jpg)
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Mouse brain tissue using anti-NeuN antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Immunohistochemistry-Paraffin-NBP2-67314-img0006.jpg)
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Rat brain tissue using anti-NeuN antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Immunohistochemistry-Paraffin-NBP2-67314-img0007.jpg)
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-RBFOX3/NeuN antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5%](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Immunohistochemistry-Paraffin-NBP2-67314-img0009.jpg)
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ROBX3/NeuN antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Immunohistochemistry-Paraffin-NBP2-67314-img0012.jpg)
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-RBFOX3/NeuN antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Immunohistochemistry-Paraffin-NBP2-67314-img0013.jpg)
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-RBFOX3/NeuN antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Immunohistochemistry-Paraffin-NBP2-67314-img0014.jpg)
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody (SR45-07) [NBP2-67314] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-RBFOX/NeuN antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-SR45-07-Immunohistochemistry-Paraffin-NBP2-67314-img0015.jpg)
![Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Western-Blot-NBP2-75397-img0008.jpg "Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Staining AIF-1/Iba1 in SH-SY5Y cells using (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunocytochemistry-Immunofluorescence-NBP2-75397-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Iba1 with Rabbit anti-AIF-1/Iba1 antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0015.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of THP-1 cells withAIF-1:Iba1 Antibody (JM36-62) at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Flow-Cytometry-NBP2-75397-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human lung cancer tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0005.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0006.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded rat lung tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded human spleen tissue using anti-AIF-1/Iba1rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4C and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0009.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded mouse brain tissue using anti-AIF-1/Iba1 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4 and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0010.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0011.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0012.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0013.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0014.jpg)
![Western Blot: DLL3 Antibody [NBP2-24669] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/mL respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DLL3-Antibody-Western-Blot-NBP2-24669-img0001.jpg "Western Blot: DLL3 Antibody [NBP2-24669] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/mL respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: DLL3 Antibody [NBP2-24669] - Human brain tissue probed with an isotype control (top) and DLL3 antibody (bottom) at 5 ug/mL.](http://images.novusbio.com/fullsize/DLL3-Antibody-Immunohistochemistry-Paraffin-NBP2-24669-img0002.jpg)
![Western Blot: Parvalbumin Antibody [NBP2-50036] - Analysis of skeletal muscle lysates and His-tagged recombinant human proteins using chicken parvalbumin pAb, dilution 1:2,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] recombinant full length human parvalbumin, [4] recombinant full length human calretinin and [5] recombinant full length human calbindin. Band at 12kDa in muscle lysate is native parvalbumin and 18kDa band in the next lane is His-tagged recombinant parvalbumin. The parvalbumin antibody binds parvalbumin strongly but is not cross-reactive with the related calcium binding proteins calretinin and calbindin.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NBP2-50036-img0005.jpg "Western Blot: Parvalbumin Antibody [NBP2-50036] - Analysis of skeletal muscle lysates and His-tagged recombinant human proteins using chicken parvalbumin pAb, dilution 1:2,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] recombinant full length human parvalbumin, [4] recombinant full length human calretinin and [5] recombinant full length human calbindin. Band at 12kDa in muscle lysate is native parvalbumin and 18kDa band in the next lane is His-tagged recombinant parvalbumin. The parvalbumin antibody binds parvalbumin strongly but is not cross-reactive with the related calcium binding proteins calretinin and calbindin.")
![Immunohistochemistry Free-Floating: Parvalbumin Antibody [NBP2-50036] - Analysis of a rat cerebellum section stained with chicken pAb to parvalbumin, NBP2-50036, dilution 1:2,000, in green, and costained with mouse mAb to GFAP, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. NBP2-50036 antibody labels the perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astrocytes.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunofluorescence-NBP2-50036-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: DISC1 Antibody [NB110-40773] - DISC1 antibody was tested in PC-12 cells at 1:100 with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).](http://images.novusbio.com/fullsize/DISC1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40773-img0006.jpg "Immunocytochemistry/Immunofluorescence: DISC1 Antibody [NB110-40773] - DISC1 antibody was tested in PC-12 cells at 1:100 with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).")
![Immunohistochemistry: DISC1 Antibody [NB110-40773] - Staining of DISC1 in human brain neurons and neuropil at 2.5ug/ml. 40X.](http://images.novusbio.com/fullsize/DISC1-Antibody-Immunohistochemistry-NB110-40773-img0005.jpg)
![Western Blot: Spectrin beta 3 Antibody (SPTBN2/1584) [NBP2-53315] - Western Blot Analysis of PANC-1 cell lysate using Spectrin beta 3 Antibody (SPTBN2/1584).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Western-Blot-NBP2-53315-img0009.jpg "Western Blot: Spectrin beta 3 Antibody (SPTBN2/1584) [NBP2-53315] - Western Blot Analysis of PANC-1 cell lysate using Spectrin beta 3 Antibody (SPTBN2/1584).")
![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (SPTBN2/1584) [NBP2-53315] - Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling Spectrin beta III with Spectrin beta 3 Antibody (SPTBN2/1584) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Immunocytochemistry-Immunofluorescence-NBP2-53315-img0006.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1584) [NBP2-53315] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Immunohistochemistry-Paraffin-NBP2-53315-img0003.jpg)
![Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1584) [NBP2-53315] - Flow Cytometric Analysis of HeLa cells using Spectrin beta 3 Antibody (SPTBN2/1584) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Flow-Cytometry-NBP2-53315-img0005.jpg)
![Western Blot: Spectrin beta 3 Antibody (SPTBN2/1584) [NBP2-53315] - Western Blot Analysis of HeLa cell lysate using Spectrin beta 3 Antibody (SPTBN2/1584).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Western-Blot-NBP2-53315-img0008.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1584) [NBP2-53315] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Immunohistochemistry-Paraffin-NBP2-53315-img0001.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1584) [NBP2-53315] - Formalin-fixed, paraffin-embedded Mouse Kidney stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Immunohistochemistry-Paraffin-NBP2-53315-img0002.jpg)
![Protein Array: Spectrin beta 3 Antibody (SPTBN2/1584) [NBP2-53315] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta 3 Antibody (SPTBN2/1584).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Protein-Array-NBP2-53315-img0010.jpg)
![Western Blot: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - Total protein from Human and Mouse Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Spectrin Beta III in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-1287A-Western-Blot-NBP2-59668-img0004.jpg "Western Blot: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - Total protein from Human and Mouse Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Spectrin Beta III in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Spectrin beta 3 (1287A) at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-1287A-Immunocytochemistry-Immunofluorescence-NBP2-59668-img0002.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human brain using Spectrin beta III antibody at a 1:1,000 dilution. The primary antibody bound to Spectrin beta III was detected with HRP-DAB detection method and the nuclei were counterstained with hematoxylin. This Spectrin beta III antibody generated a specific cytoplasmic staining in all the cells with strongest signal in neuronal cells.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-1287A-Immunohistochemistry-Paraffin-NBP2-59668-img0005.jpg)
![Flow (Intracellular): Spectrin beta 3 Antibody (1287A) [NBP2-59668] - An intracellular stain was performed on HeLa Cells with Spectrin beta 3 (1287A) antibody NBP2-59668 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-1287A-Flow-Intracellular-NBP2-59668-img0001.jpg)