| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64895SS | |||
| Azide and BSA Free | NBP2-80651 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-892SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-78486SS | |||
| Azide and BSA Free | NBP2-80884 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-28904SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-317SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87102-25ul | |||
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using Alexa Fluor (R) 488 conjugate of CD45 antibody (OX-1). Rat Splenocytes were stained with CD45 antibody (OX-1) [NB100-64895] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0005.jpg "Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using Alexa Fluor (R) 488 conjugate of CD45 antibody (OX-1). Rat Splenocytes were stained with CD45 antibody (OX-1) [NB100-64895] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using the FITC conjugate of CD45 antibody (OX-1). Staining of rat spleen cells.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0004.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) - BSA Free [NB100-64895] - Representative sequential gating during flow cytometry analysis of rat non-parenchymal liver cells (n = 4). Cells were labeled with antibodies to SE-1 (NB110-68095AF488), CD31, and CD45 (NB100-64895PE). LSECs were then identified as SE-1-Alexa488+ cells (e), and the biexponential CD45-PE/CD31-APC of events (f) were used to display and select the CD45+ CD31+ subsets of LSECs. FMOs (used for gating), and single antibody staining controls are shown in Additional file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33246411/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-BSA-Free-Flow-Cytometry-NB100-64895-img0006.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Staining of rat peripheral blood lymphocytes with mouse anti-rat CD45 antibody (OX-1) [NB100-64895].](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0001.jpg)
![Western Blot: DLL4 Antibody [NB600-892] - Western Blot of DLL4 antibody. Lane 1: Mouse Pancreas Whole Cell Lysate. Lane 2: HUVEK Whole Cell Lysate.Lane 3: Human rDLL4.Lane 4: HEK293 Whole Cell Lysate + Human rDLL4. Lane 5: HEK293 Whole Cell Lysate.Load: 10 ug per lane for Whole Cell Lysates or 50 ng for recombinant protein.Primary antibody: DLL4 antibody at 1:1,000 overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 74 kDa, 74 kDa for DLL4. Other band(s): Not Identified.](http://images.novusbio.com/fullsize/DLL4-Antibody-Western-Blot-NB600-892-img0006.jpg "Western Blot: DLL4 Antibody [NB600-892] - Western Blot of DLL4 antibody. Lane 1: Mouse Pancreas Whole Cell Lysate. Lane 2: HUVEK Whole Cell Lysate.Lane 3: Human rDLL4.Lane 4: HEK293 Whole Cell Lysate + Human rDLL4. Lane 5: HEK293 Whole Cell Lysate.Load: 10 ug per lane for Whole Cell Lysates or 50 ng for recombinant protein.Primary antibody: DLL4 antibody at 1:1,000 overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 74 kDa, 74 kDa for DLL4. Other band(s): Not Identified.")
![Immunohistochemistry: DLL4 Antibody [NB600-892] - Affinity Purified DLL4 antibody was used at 20 ug/ml to detect DLL4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows DLL4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/DLL4-Antibody-Immunohistochemistry-NB600-892-img0007.jpg)
![Western Blot: DLL4 Antibody [NB600-892] - Western Blot of DLL4 antibody. Lane 1: mouse pancreatic tissue. Load: 20 ug per lane.Primary antibody: DLL4 antibody at 1:500 for overnight at 4C.Secondary antibody: HRP-labeled Goat anti-Rabbit IgG secondary antibody at 1:5,000 for 1 hour at RT. A chemiluminescence system was used for signal detection using a 3 min exposure time.Block: 5% Goat Serum overnight at 4C.Predicted/Observed size: 74 kDa, 70 kDa for Delta 4. Other band(s): none.](http://images.novusbio.com/fullsize/DLL4-Antibody-Western-Blot-NB600-892-img0005.jpg)
![ELISA: DLL4 Antibody [NB600-892] - ELISA results of purified DLL4 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/DLL4-Antibody-ELISA-NB600-892-img0008.jpg)
![SDS-Page: Recombinant Human BMAL1 Protein [H00000406-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-BMAL1-Protein-SDS-Page-H00000406-P01-img0002.jpg "SDS-Page: Recombinant Human BMAL1 Protein [H00000406-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Knockout-Validated-NB100-78486-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human 270 kDa full-length NOTCH1 and 110-120 kDa cleaved NOTCH 1 (NICD).](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Western-Blot-NB100-78486-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (mN1A) [NB100-78486] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution with DyLight 488 (Green). Actin was counterstained with Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Immunocytochemistry-Immunofluorescence-NB100-78486-img0008.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (mN1A) [NB100-78486] - Analysis of FFPE human pancreatic cancer using 1:10 dilution of Notch-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in epithelial cells was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Immunohistochemistry-Paraffin-NB100-78486-img0010.jpg)
![Flow Cytometry: Notch-1 Antibody (mN1A) [NB100-78486] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Flow-Cytometry-NB100-78486-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28904-img0009.jpg "Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. IHC localization of LGR5 in the ZG. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 um) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients and two from pheochromocytoma patients. Scale bar, 500 um (low power) and 100 um (high power). C, capsule. Image collected and cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0011.jpg)
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Analysis of human small intestine.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0005.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. A, Heat map of 37 genes (shown on the vertical axis), which are 5-fold or greater differentially expressed in ZG & ZF. Genes previously associated with the Wnt signaling pathway are highlighted in red. On the horizontal axis, each pair of ZG & ZF from the 20 adrenals are anonymized & numbered. Probe set & detailed microarray information are available from the National Center for Biotechnology Information Gene Expression Omnibus under accession number GSE64957. B, IHC localization of LGR5 & downstream Wnt signaling proteins in the ZG: from the canonical beta -catenin pathway, beta -catenin & LEF1, & from the noncanonical AP1/JUN pathway, c-JUN & p-c-JUN. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 μm) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Interestingly, beta -catenin staining was mostly membranous or cytoplasmic (rather than nuclear staining as highlighted by the arrows), indicating limited canonical Wnt activation. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients & two from pheochromocytoma patients. Scale bar, 500 μm (low power) & 100 μm (high power). C, capsule. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28904_rabbit-polyclonal-lgr5-gpr49-antibody-31020241618943.jpg)
![Simple Western: Ki67/MKI67 Antibody [NBP2-19012] - Detection of Ki67/MKI67 by Simple WesternTM. Simple Western lane view shows lysates of HeLa parental cell line and Ki67 knockout (KO) HeLa cell line. A specific band was detected for Ki67/MKI67 at approximately 312 kDa (as indicated) in the parental cell line, but is not detectable in the knockout HeLa cell line using 20 ug/mL of Rabbit Anti-Ki67/MKI67 Polyclonal Antibody (Catalog # NBP2-19012). GAPDH is shown as a loading control. This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Knockout-Validated-NBP2-19012-img0009.jpg "<b>Genetic Strategies Validation. </b>Simple Western: Ki67/MKI67 Antibody [NBP2-19012] - Detection of Ki67/MKI67 by Simple WesternTM. Simple Western lane view shows lysates of HeLa parental cell line and Ki67 knockout (KO) HeLa cell line. A specific band was detected for Ki67/MKI67 at approximately 312 kDa (as indicated) in the parental cell line, but is not detectable in the knockout HeLa cell line using 20 ug/mL of Rabbit Anti-Ki67/MKI67 Polyclonal Antibody (Catalog # NBP2-19012). GAPDH is shown as a loading control. This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NBP2-19012] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP2-19012 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19012-img0010.jpg)
![Western Blot: Ki-67/MKI67 Antibody [NBP2-19012] - Analysis of A431 (A), HeLa (B), Ntera2 (C), and HEK293 (D) cell lysate using Ki67 antibody (NBP2-19012) at 2 ug/ml.](http://images.novusbio.com/fullsize/Ki67-Antibody-Western-Blot-NBP2-19012-img0006.jpg)
![Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NBP2-19012] - Human breast tumor stained with Ki-67 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/Ki67-Antibody-Immunohistochemistry-Paraffin-NBP2-19012-img0003.jpg)
![Flow Cytometry: Ki-67/MKI67 Antibody [NBP2-19012] - Expression in actively growing Jurkat cells: Cells were stained with 0.2 ug of Ki-67 antibody (red) and isotype control (green) and positively stained population was identified using PE conjugated goat anti-rabbit IgG secondary antibody. Cells fixed and permeabilized using ice cold 70% ethanol were used in this intracellular staining protocol.](http://images.novusbio.com/fullsize/Ki67-Antibody-Flow-Cytometry-NBP2-19012-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ki-67/MKI67 Antibody [NBP2-19012] - Ki67 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.](http://images.novusbio.com/fullsize/Ki67-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19012-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Ki67/MKI67 Antibody [NBP2-19012] - Cell proliferation of BCa cells treated by SIRT1-target-specific-siRNA (B) and negative-control-siRNA (A) was assayed by Ki-67 staining (green). Nuclei (blue) were stained by DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29147649/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19012-img0011.jpg)
![Western Blot: GFAP Antibody [NB100-53809] - (0.005ug/ml) staining of Human Cerebellum (A) and (0.01ug/ml) Cerebral Cortex (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NB100-53809-img0002.jpg "Western Blot: GFAP Antibody [NB100-53809] - (0.005ug/ml) staining of Human Cerebellum (A) and (0.01ug/ml) Cerebral Cortex (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: GFAP Antibody [NB100-53809] - (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NB100-53809-img0001.jpg)
![Western Blot: xCT Antibody [NB300-317] - Total protein from human HeLa, Huvec, HCT-116 and A549 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-xCT in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/xCT-Antibody-Western-Blot-NB300-317-img0011.jpg "Western Blot: xCT Antibody [NB300-317] - Total protein from human HeLa, Huvec, HCT-116 and A549 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-xCT in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: xCT Antibody [NB300-317] - xCT expression is enhanced in CD68+ cells from MS spinal cord. CD68+ cells (arrows) show enhanced xCT expression in MS patients as compared to controls. CD68+ macrophages are round shaped and form clusters in MS patients, whereas in controls, CD68+ cells appear isolated and long shaped. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-63), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/xCT-Antibody-Immunohistochemistry-Paraffin-NB300-317-img0015.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: xCT Antibody [NB300-317] - Total protein from Human HeLa cells treated with and without 0.1 mM Diethyl Maleate for 24 hours was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-xCT in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the increase in xCT expression with treatment.](http://images.novusbio.com/fullsize/xCT-Antibody-Western-Blot-NB300-317-img0012.jpg)
![Flow Cytometry: xCT Antibody [NB300-317] - An intracellular stain was performed on HeLa cells with NB300-317G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.](http://images.novusbio.com/fullsize/xCT-Antibody-Flow-Cytometry-NB300-317-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: xCT Antibody [NB300-317] - xCT antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/xCT-Antibody-Immunocytochemistry-Immunofluorescence-NB300-317-img0005.jpg)
![Flow Cytometry: xCT Antibody [NB300-317] - An intracellular stain was performed on HeLa with NB300-317 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/xCT-Antibody-Flow-Cytometry-NB300-317-img0013.jpg)
![Simple Western: xCT Antibody [NB300-317] - (1) ladder, (2) no lysate + xCT, 100ug/ml, (3) human brain frontal cortex membrane lysate,no primary antibody, (4-7) human brain lysates, 0.03mg/ml, xCT, 100ug/ml.](http://images.novusbio.com/fullsize/xCT-Antibody-Simple-Western-NB300-317-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody - BSA Free [NB300-317] - xCT expression is enhanced in CD68+ cells from MS spinal cord. A. Triple immunofluorescence staining for xCT (green), CD68 (red) & Hoechst 33258 (blue) in spinal cord of control (left) & MS patients (right). A high expression of xCT was detected in CD68+ infiltrating macrophages (arrows) associated with blood vessels, which are virtually absent in controls. Note that overall xCT expression is enhanced in MS tissue. B. CD68+ cells (arrows) show enhanced xCT expression in MS patients as compared to controls. CD68+ macrophages are round shaped & form clusters in MS patients, whereas in controls, CD68+ cells appear isolated & long shaped. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-63), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-317_rabbit-polyclonal-xct-antibody-310202415284530.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody - BSA Free [NB300-317] - xCT expression is enhanced in CD68+ cells from MS spinal cord. A. Triple immunofluorescence staining for xCT (green), CD68 (red) & Hoechst 33258 (blue) in spinal cord of control (left) & MS patients (right). A high expression of xCT was detected in CD68+ infiltrating macrophages (arrows) associated with blood vessels, which are virtually absent in controls. Note that overall xCT expression is enhanced in MS tissue. B. CD68+ cells (arrows) show enhanced xCT expression in MS patients as compared to controls. CD68+ macrophages are round shaped & form clusters in MS patients, whereas in controls, CD68+ cells appear isolated & long shaped. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-63), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-317_rabbit-polyclonal-xct-antibody-310202415392541.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody - BSA Free [NB300-317] - xCT expression is increased in the CNS of rats with EAE. A. Histogram showing the neurological score during the course of acute EAE induced in Lewis rats by immunization with myelin basic protein. The peak of neurological disability was at day 14 post-immunization, which was selected for obtaining tissue samples. B. xCT mRNA (left) & protein (right) expression in spinal cord from control & acute EAE rats, as assessed by qPCR & Western blot analysis. Data are referred to mean expression level of controls (n = 5-6). C. Double immunofluorescence for xCT (green) & OX-42 (red), a marker of microglia & infiltrating macrophages. OX42+ cells express high xCT levels in acute EAE. Both meninges (asterisk in top) & infiltrating cells (bottom) in inflammatory foci show high levels of xCT in rat spinal cord with EAE as compared to controls. D. Microglial cells (OX42+ cells) of EAE rats have higher xCT levels in spinal cord than controls. Notice the difference between resting microglia in control rats, with ramified morphology (arrows in control) & microglia in EAE showing round shaped morphology, characteristic of its activated state (arrows in EAE). Scale bar = 20 μm.E. xCT mRNA (left) & protein (right) expression in spinal cord from control & chronic EAE mice, assessed by qPCR & Western blot analysis. Data are referred to mean expression level of controls (n = 5). Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-63), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-317_rabbit-polyclonal-xct-antibody-310202416205190.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody - BSA Free [NB300-317] - xCT expression is increased in the CNS of rats with EAE. A. Histogram showing the neurological score during the course of acute EAE induced in Lewis rats by immunization with myelin basic protein. The peak of neurological disability was at day 14 post-immunization, which was selected for obtaining tissue samples. B. xCT mRNA (left) & protein (right) expression in spinal cord from control & acute EAE rats, as assessed by qPCR & Western blot analysis. Data are referred to mean expression level of controls (n = 5-6). C. Double immunofluorescence for xCT (green) & OX-42 (red), a marker of microglia & infiltrating macrophages. OX42+ cells express high xCT levels in acute EAE. Both meninges (asterisk in top) & infiltrating cells (bottom) in inflammatory foci show high levels of xCT in rat spinal cord with EAE as compared to controls. D. Microglial cells (OX42+ cells) of EAE rats have higher xCT levels in spinal cord than controls. Notice the difference between resting microglia in control rats, with ramified morphology (arrows in control) & microglia in EAE showing round shaped morphology, characteristic of its activated state (arrows in EAE). Scale bar = 20 μm.E. xCT mRNA (left) & protein (right) expression in spinal cord from control & chronic EAE mice, assessed by qPCR & Western blot analysis. Data are referred to mean expression level of controls (n = 5). Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-63), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-317_rabbit-polyclonal-xct-antibody-310202416205138.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0031.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Cellular aggregates composed of equine endometrial fibroblast. Co-staining using the vimentin antibody and a cytokeratin antibody (mouse). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunocytochemistry-NBP1-31327-img0037.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Human primary cardiac fibroblasts stained with Vimentin (green) and smooth muscle actin (red) antibodies. Image from verified customer review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31327-img0024.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat ovary. Vimentin antibody at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0036.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - 293T cell lysate: A. 20 ug. B. 10 ug. C. 5 ug. D. 1 ug.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0014.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, membrane was blotted with Vimentin antibody at 1:50000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0023.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded human lung adenocarcinoma. Vimentin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0022.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat testis. Vimentin antibody at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0034.jpg)
![Immunoprecipitation: Vimentin Antibody [NBP1-31327] - Vimentin protein from HeLa whole cell extracts using 5 ug of Vimentin antibody. Western blot analysis was performed using Vimentin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunoprecipitation-NBP1-31327-img0019.jpg)
![Sandwich ELISA: Vimentin Antibody [NBP1-31327] - Detection of recombinant full-length Vimentin protein using as capture antibody at concentration of 5 ug/mL as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Sandwich-ELISA-NBP1-31327-img0039.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-9102024204128.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-91020242010448.jpg)
![ELISA: Vimentin Antibody [NBP1-31327] - Sandwich ELISA detection of recombinant full-length Vimentin protein using the capture antibody at concentration of 5 ug/mL and NBP1-31327 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-91020242063381.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202419593530.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420131228.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.Sample:Paraffin-Embedded adult mouse retina. Green: Vimentin protein stained by Vimentin antibody (NBP1-31327) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420131227.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-9102024201149.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoskeleton and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-91020242011456.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-91020242021974.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat ovary.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420134066.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420134020.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein by immunohistochemical analysis.Sample: Paraffin-embedded dog pancreas.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420115052.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoskeleton by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420134098.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP1-31327] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-1610202419414514.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood lymphocytes with anti-human CD45 antibody (MEM-28) Alexa Fluor 700.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0021.jpg "Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood lymphocytes with anti-human CD45 antibody (MEM-28) Alexa Fluor 700.")
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (MEM-28) [NB500-319] - staining of human peripheral blood mononuclear cell using anti-human CD45 antibody (MEM-28) (green). DNA visualized by DAPI (blue).](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Immunocytochemistry-NB500-319-img0001.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE-CyTM5.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0018.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (MEM-28) [NB500-319] - Staining of human small intestine (paraffin-embedded sections) with CD45 antibody (MEM-28), 10 ug/ml.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Immunohistochemistry-Paraffin-NB500-319-img0022.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0007.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) purified, GAM-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0008.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) APC-CyTM7.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0010.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0016.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0017.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE-CyTM7.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0019.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE-DyLight (R) 594.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0020.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Analysis in human cerebral cortex and liver tissues. Corresponding S100B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Analysis in human cerebral cortex and liver tissues. Corresponding S100B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: S100B Antibody [NBP1-87102] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-Western-Blot-NBP1-87102-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody [NBP1-87102] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining Is shown in green.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87102-img0018.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0025.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in purkinje cell layer.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0022.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0023.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0024.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term diluted at 1:3000.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0033.jpg "Western Blot: beta-Catenin Antibody [NBP1-32239] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term diluted at 1:3000.")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - Paraformaldehyde-fixed A431, using beta- Catenin antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32239-img0017.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - WNT10A/beta-catenin signaling is required for region-specific differentiation. Epithelial deletion of beta-catenin (g-h](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0043.jpg)
![Chromatin Immunoprecipitation (ChIP): beta-Catenin Antibody [NBP1-32239] - Cross-linked ChIP was performed with HCT116 chromatin extract and 5 ug of either control rabbit IgG or anti-beta Catenin antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-32239-img0024.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: beta-Catenin Antibody [NBP1-32239] - Western blot for beta-catenin in HEK 293 cells treated with LiCl (10mM) for 1 hr. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0008.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0013.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - A. 30 ug PC-12 whole cell lysate/extract 7.5 % SDS-PAGEbeta Catenin antibody [N1N2-2], N-term dilution: 1:1000](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody [NBP1-32239] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32239-img0032.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse urinary bladder diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0019.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse skin dilution: 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0020.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse colon dilution: 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0021.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0036.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded human esophagus. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0037.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded human cervix. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0038.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0039.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded rat colon. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0040.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded rat duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0041.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse intestine. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0042.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - P-cadherin is co-localized with other junctional proteins at the RPE cell border in mice.Immunofluorescence of mouse RPE flat-mounts. Double staining: P-cadherin (red; A, E, I) & either ZO-1 (green; B), beta -catenin (green; F), or F-actin (green; J), with nuclear stain by DAPI (blue; C, G, K). Merged images (D, H, L) show the co-localization of P-cadherin with ZO-1 (tight junction), beta -catenin (adherens junction), & F-actin (adherens junction) at the cell-cell border. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29338041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-310202415171344.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-31020241617146.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-310202416171420.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-31020241618265.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-31020241618934.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - Oxidative stress-induced dissociation of adherens junctions results in nuclear translocation of beta -catenin & an increase of EMT-related factors in mouse RPE.(A) Immunofluorescence of mouse RPE flat-mounts. Mice were injected with NaIO3 (15 mg/kg body weight) on Day 0, & the localization of beta -catenin (green) & P-cadherin (red) was analyzed along with nuclear stain by DAPI (blue) on Days 0 (a-c), 1 (d-f), 3 (g-i) & 7 (j-l). Double staining: beta -catenin (a, d, g, j), P-cadherin (b, e, h, k), & merged images with DAPI (c, f, i, l). The localization of beta -catenin & P-cadherin at the cell-cell border was significantly disrupted, & instead prominently detected on/in the nucleus on Day 3. (B) Immunofluorescence of mouse retinal sections with a focus on the RPE nuclei. Mice were injected with NaIO3 (15 mg/kg body weight) on Day 0, & the localization of beta -catenin (green) & P-cadherin (red) was analyzed along with nuclear stain by DAPI (blue) on Days 0 (m-o) & 3 (two representative nuclei; p-r & s-u). Double staining: beta -catenin (m, p, s), P-cadherin (n, q, t), & merged images with DAPI (o, r, u). On Day 3, beta -catenin was detected in the nuclei of mouse RPE. (C) Western blot analyses of mouse RPE proteins. Mice were injected with NaIO3 (15 mg/kg body weight) on Day 0, & RPE protein lysates were prepared on Days 0, 1, 3, & 7. The protein levels were analyzed using Western blotting with antibodies against P-cadherin, beta -catenin, SNAI1 (Snail), vimentin, & control beta -actin. The protein levels of beta -catenin & SNAI1 increased similarly on Day 1 following oxidative stress. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29338041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-310202416165641.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP1-32239) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242011415.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.beta-Catenin stained by beta-Catenin antibody (NBP1-32239) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242075974.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded cat colon.beta-Catenin stained by beta-Catenin antibody (NBP1-32239) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420122025.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420134025.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Various tissue extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP1-32239) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420104461.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - Immunohistochemical analysis of agarose-embedded zebrafish embryo, using beta-Catenin antibody [N1N2-2], N-term NBP1-32239) at 1:100. dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420115056.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 1 day-post-fertilization zebrafish embryo. beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420564.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects beta-Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.beta-Catenin stained by beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-9102024202461.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2] detects beta-Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: beta-Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500.Red: alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Hoechst 33342 staining.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242001070.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-9102024202198.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta-Catenin stained by beta-Catenin antibody (NBP1-32239) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242010443.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242075983.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects beta-Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.beta-Catenin stained by beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242075987.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP1-32239) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420131247.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody [NBP1-32239] - Wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts (9 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-1610202419405728.jpg)
![beta-Catenin antibody [N1N2-2] detects beta-Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: beta-Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500.Red: alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Hoechst 33342 staining.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-immunohistochemistry-paraffin-1411202518321620.jpg)
![beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) detects CTNNB1 protein by flow cytometry analysis. Sample: HeLa cell. Black: Unlabelled sample was used as a control. Red: beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-flow-cytometry-1411202518333124.jpg)
![beta-Catenin antibody [N1N2-2], N-term detects beta-Catenin protein at cell membrane by immunofluorescent analysis.Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta-Catenin protein stained by beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-immunocytochemistry-immunofluorescence-1411202518324119.jpg)
