| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33186 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74570SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23489SS | |||
| Azide and BSA Free | NBP2-80702 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24691SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1598SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56326SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25146SS | |||
![Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (13C4)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Western-Blot-NBP2-29420-img0007.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (13C4)")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (13C4) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Immunocytochemistry-Immunofluorescence-NBP2-29420-img0006.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Simple-Western-NBP2-29420-img0001.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Electropherogram image of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 lysates(s) respectively.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Simple-Western-NBP2-29420-img0004.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of different tissue and cell lysates using rabbit pAb to UCHL1, NB110-58874, dilution 1:2,000 in red, and mouse mAb to HSP60, dilution 1:10,000, in green: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24kDa corresponds to the UCHL1 protein, while the 60kDa band represents HSP60 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties but not in HeLa, NIH-3T3 and other non-neuronal cells.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NB110-58874-img0006.jpg "Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of different tissue and cell lysates using rabbit pAb to UCHL1, NB110-58874, dilution 1:2,000 in red, and mouse mAb to HSP60, dilution 1:10,000, in green: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24kDa corresponds to the UCHL1 protein, while the 60kDa band represents HSP60 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties but not in HeLa, NIH-3T3 and other non-neuronal cells.")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of SH-SY5Y cells stained with rabbit pAb to UCHL1, NB110-58874, dilution 1:1,000 in green, and costained with mouse mAb to fibrillarin, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cellular cytoplasm of these cells which share many properties with neurons, while the fibrilarin antibody specifically labels nucleoli.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58874-img0005.jpg)
![Western Blot: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Blot of whole bovine brain extract stained with RPCA-UCHL1 showing a strong and clean band at about 24kDa.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB110-58874-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Shows rat mixed neuron/glial cultures stained with Rabbit anti-UCHL1 antibody (green). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58874-img0003.jpg)
![Simple Western: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Simple Western lane view shows a specific band for PGP9.5/UCHL-1 in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Simple-Western-NB110-58874-img0004.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse colon. NRF1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP2-19586-img0010.jpg "Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse colon. NRF1 antibody dilution: 1:500.")
![Western Blot: Nrf1 Antibody [NBP2-19586] - IP samples: 293T whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of NRF1 protein by 4 ug NRF1 antibody. 10 % SDS-PAGE The immunoprecipitated NRF1 protein was detected by NRF1 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent].](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP2-19586-img0003.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded human lung adenocarcinoma. NRF1 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP2-19586-img0006.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse muscle. NRF1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP2-19586-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf1 Antibody [NBP2-19586] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF1 stained by NRF1 antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Scale bar= 10μm.](http://images.novusbio.com/fullsize/antibody/nbp2-19586_rabbit-polyclonal-nrf1-antibody-immunocytochemistry-immunofluorescence-582024111857..jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Nrf1 antibody detects Nrf1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Nrf1 stained by Nrf1 antibody (NBP2-19586) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19586_rabbit-polyclonal-nrf1-antibody-91020242091460.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053] - Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma. Oct4 stained by Oct4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/OCT4-Antibody-Immunohistochemistry-Paraffin-Negative-NBP2-15053-img0015.jpg "Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053] - Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma. Oct4 stained by Oct4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Flow Cytometry: OCT4 Antibody [NBP2-15053] - Human embryonic stem cells, staining with OCT3/4 or rabbit IgG (black).](http://images.novusbio.com/fullsize/OCT4-Antibody-Flow-Cytometry-NBP2-15053-img0001.jpg)
![Western Blot: OCT4 Antibody [NBP2-15053] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NBP2-15053-img0007.jpg)
![Western Blot: OCT4 Antibody [NBP2-15053] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NBP2-15053-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NBP2-15053] - Analysis of hESCs on CF1 feeders, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/OCT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15053-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: OCT4 Antibody [NBP2-15053] - Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15053_rabbit-polyclonal-oct4-antibody-1610202419414555.jpg)
![Western Blot: CHT1 Antibody (62-2E8) [NB110-74570] - Western Blot Image of anti-CHT1 (62-2E8). Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:500 dilution of anti-CHT1 in 1% milk/TBST. Reactivity was detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CHT1-Antibody-62-2E8-Western-Blot-NB110-74570-img0003.jpg "Western Blot: CHT1 Antibody (62-2E8) [NB110-74570] - Western Blot Image of anti-CHT1 (62-2E8). Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:500 dilution of anti-CHT1 in 1% milk/TBST. Reactivity was detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry: CHT1 Antibody (62-2E8) [NB110-74570] - Staining of neurons in rat basal forebrain](http://images.novusbio.com/fullsize/CHT1-Antibody-62-2E8-Immunohistochemistry-NB110-74570-img0002.jpg)
![Western Blot: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis using DRP1 mouse mAb in (1) A549 and (2) Jurkat cell lysates.](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Western-Blot-NBP2-23489-img0003.jpg "Western Blot: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis using DRP1 mouse mAb in (1) A549 and (2) Jurkat cell lysates.")
![Immunohistochemistry: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis of paraffin-embedded human cerebellum tissues using DRP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Immunohistochemistry-NBP2-23489-img0005.jpg)
![Western Blot: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis using DRP1 mAb in human DRP1 recombinant protein. (Expected MW is 41.8 kDa)](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Western-Blot-NBP2-23489-img0001.jpg)
![Western Blot: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis using DRP1 mAb in (1) HEK293 and (2) DRP1 hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Western-Blot-NBP2-23489-img0002.jpg)
![Immunohistochemistry: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis of paraffin-embedded human ovarian cancer tissues using DRP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Immunohistochemistry-NBP2-23489-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MNX1/HLXB9 Antibody [NBP2-24691] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MNX1/HLXB9 NBP2-24691 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24691-img0003.jpg "Immunocytochemistry/Immunofluorescence: MNX1/HLXB9 Antibody [NBP2-24691] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MNX1/HLXB9 NBP2-24691 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: MNX1/HLXB9 Antibody [NBP2-24691] - Neuro2a cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MNX1/HLXB9 NBP2-24691 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24691-img0004.jpg)
![Western Blot: MNX1/HLXB9 Antibody [NBP2-24691] - Analysis of MNX1/HLXB9 in MOLT-4 cell lysates in the 1) absence and 2) presence of immunizing peptide using this antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Western-Blot-NBP2-24691-img0002.jpg)
![Immunohistochemistry-Paraffin: MNX1/HLXB9 Antibody [NBP2-24691] - Analysis of MNX1/HLXB9 in FFPE mouse pancreas tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Immunohistochemistry-Paraffin-NBP2-24691-img0001.jpg)
![Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Western-Blot-NB100-1598-img0013.jpg "Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1598-img0010.jpg)
![Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on Jurkat with NB100-1598 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Flow-Cytometry-NB100-1598-img0012.jpg)
![Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on HeLa cells with NB100-1598PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Flow-Cytometry-NB100-1598-img0011.jpg)
![Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-12793-img0002.jpg "Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500. Image submitted by a verified customer review.")
![Immunoprecipitation: Insulin Receptor beta Antibody [NBP2-12793] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Insulin Receptor Beta antibody NBP2-12793 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Insulin Receptor Beta was also immunoprecipitated by rabbit anti-Insulin Receptor Beta antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-Immunoprecipitation-NBP2-12793-img0001.jpg)
![Western Blot: MeCP2 Antibody [NB100-56326] - Analysis of MeCP2 in HeLa cell lysate using this antibody.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NB100-56326-img0002.jpg "Western Blot: MeCP2 Antibody [NB100-56326] - Analysis of MeCP2 in HeLa cell lysate using this antibody.")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB100-56326] - This antibody was tested in A431 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56326-img0003.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Mouse liver tissue using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0007.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Tissue section of mouse pancreas using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in majority of the cells from acini, ducts and Islets of Langerhans in the section.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0004.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Mouse liver tissue using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunofluorescence-NBP2-25146-img0010.jpg "Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.")
![Western Blot: alpha-Synuclein Antibody [NBP2-25146] - Western blot analysis of different tissue lysates using chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the alpha-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-25146-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody [NBP2-25146] - Mixed rat neuron-glial cultures stained with NBP2-25146, polyclonal antibody to alpha-Synuclein (red) and monoclonal antibody to MAP2 (NBP2-25156, green). The alpha-Synuclein antibody stains vesicular structures, the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-Synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody [NBP2-25146] - Human iPS derived neurons, fixed in 4% formaldehyde solution. NBP2-25146 diluted 1:1500, followed by staining with Alexa anti chicken-594 (1:1000) secondary antibody. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics). Image from verified customer review.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0006.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0018.jpg "Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: anti p65 NLS specific Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. The different staining patterns are thought to correspond with different functional regions of the protein.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0020.jpg)
![Immunohistochemistry: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. Showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissues such as spleen, thymus, and tonsil. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunohistochemistry-NBP1-77815-img0019.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TBST for 30 min at RT. Blot was probed with monoclonal anti-NFkB p65 at 1:1000 in 1% BSA-TBST at 4C and detected with HRP conjugated Rb-anti-Mouse antibody at 1:40,000 in for 30 min at RT.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Used at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Mouse endothelial cells. Unstimulated control cells (left) show cytoplasmic staining, TNF-alpha stimulated cells (right) show nuclear staining. For staining, cells were washed with PBS to remove all traces of culture media and fixed with paraformaldehyde 4 % (45 min). Slides were washed with PBT (phosphate buffer 0.1M + Triton-X-100 0.1 %), 3 times 10 min each, then, blocked with PBT + 5 % normal goat serum, 1 hour. Sample was Incubated overnight in primary antibody (1:600 in blocking buffer). After 3X wash in PBT for 10 min, slides were incubated 1 hour with secondary antibody (1:1000) and mounted in 1:1 PB glycerol.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect p65 by immunofluorescence at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in widefiled, confocal and STED CW imaging on the same system.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0015.jpg)
