| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-65956-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89218-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43790 | |||
| BSA Free | NBP2-46630-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71331 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70574 | |||
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining in human cerebral cortex and skin tissues.. Corresponding NCAM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining in human cerebral cortex and skin tissues.. Corresponding NCAM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NCAM-1/CD56 Antibody [NBP2-38452] - Analysis in human brain tissue.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Western-Blot-NBP2-38452-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38452-img0004.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human skin shows no positivity in keratinocytes.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0015.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0012.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0013.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP2-38452] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-Immunohistochemistry-Paraffin-NBP2-38452-img0014.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0009.jpg "Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.")
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0001.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0002.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PerCP-CyTM5.5](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0003.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) APC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0004.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PerCP.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0005.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) FITC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0006.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE-CyTM7.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0007.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0008.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0018.jpg "Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: anti p65 NLS specific Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. The different staining patterns are thought to correspond with different functional regions of the protein.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0020.jpg)
![Immunohistochemistry: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. Showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissues such as spleen, thymus, and tonsil. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunohistochemistry-NBP1-77815-img0019.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TBST for 30 min at RT. Blot was probed with monoclonal anti-NFkB p65 at 1:1000 in 1% BSA-TBST at 4C and detected with HRP conjugated Rb-anti-Mouse antibody at 1:40,000 in for 30 min at RT.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Used at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Mouse endothelial cells. Unstimulated control cells (left) show cytoplasmic staining, TNF-alpha stimulated cells (right) show nuclear staining. For staining, cells were washed with PBS to remove all traces of culture media and fixed with paraformaldehyde 4 % (45 min). Slides were washed with PBT (phosphate buffer 0.1M + Triton-X-100 0.1 %), 3 times 10 min each, then, blocked with PBT + 5 % normal goat serum, 1 hour. Sample was Incubated overnight in primary antibody (1:600 in blocking buffer). After 3X wash in PBT for 10 min, slides were incubated 1 hour with secondary antibody (1:1000) and mounted in 1:1 PB glycerol.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect p65 by immunofluorescence at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in widefiled, confocal and STED CW imaging on the same system.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0015.jpg)
![Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (1D11) - Low Endotoxin [NB100-65956] - Analysis of human tonsil tissue using NKG2D/CD314 antibody. PFA fixed paraffin-embedded sections. Heat-mediated antigen retrieval was performed for 30 minutes in citrate buffer. Primary antibody dilution was 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Low-Endotoxin-Immunohistochemistry-Paraffin-NB100-65956-img0013.jpg "Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (1D11) - Low Endotoxin [NB100-65956] - Analysis of human tonsil tissue using NKG2D/CD314 antibody. PFA fixed paraffin-embedded sections. Heat-mediated antigen retrieval was performed for 30 minutes in citrate buffer. Primary antibody dilution was 1:200. Image from verified customer review.")
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Analysis using the Biotin conjugate of NB100-65956. Staining of Human peripheral blood lymphocytes with biotinylated 1D11, followed by Sav-PE.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0008.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody,](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0009.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody,](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0010.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0011.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0012.jpg)
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD314:Low Endotoxin.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0004.jpg)
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0005.jpg)
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0006.jpg)
![Western Blot: CRHR1/CRF1 Antibody [NBP1-00175] - Staining of Human Colon (A), Cerebellum (B), Rat Brain (C) and Mouse spinal Cord (D) lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CRHR1-CRF1-Antibody-Western-Blot-NBP1-00175-img0005.jpg "Western Blot: CRHR1/CRF1 Antibody [NBP1-00175] - Staining of Human Colon (A), Cerebellum (B), Rat Brain (C) and Mouse spinal Cord (D) lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/CRHR1-CRF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00175-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175] - Immunofluorescence analysis of paraformaldehyde fixed Neuro2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane, and vesicle staining. The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/CRHR1-CRF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00175-img0006.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0002.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.")
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human spleen with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0001.jpg)
![Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Western-Blot-NBP2-46630-img0008.jpg "Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunocytochemistry/Immunofluorescence: MBP Antibody (CL2819) [NBP2-46630] - Staining of A549 cells using the Anti-MBP monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunocytochemistry-Immunofluorescence-NBP2-46630-img0009.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human tonsil shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0006.jpg)
![Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Western-Blot-NBP2-46630-img0007.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of rat cerebral cortex shows strong immunoreactivity in myelinated neural fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0001.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of mouse cerebral cortex shows strong positivity in myelinated neural fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0002.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human cerebral cortex shows strong immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0003.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of mouse striatum shows strong positivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0004.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human cerebellum shows strong immunoreactivity in myelinated neural fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0005.jpg)
![Western Blot: CD45 Antibody (BL-178-12C7) [NBP3-14668] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 1 second. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/CD45-Antibody-BL-178-12C7-Western-Blot-NBP3-14668-img0013.jpg "Western Blot: CD45 Antibody (BL-178-12C7) [NBP3-14668] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 1 second. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody [BL-178-12C7](A700-012). Secondary: HRP-conjugated goat anti-rabbitIgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0006.jpg)
![Immunohistochemistry: CD45 Antibody [NBP3-14668] - Sample: FFPE section of human coloncarcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™.Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0007.jpg)
![Flow Cytometry: CD45 Antibody [NBP3-14668] - Sample: Jurkat cells were fixed in 4% formaldehyde andpermeabilized with 90% methanol. Antibody:1E6 cells were incubated with 1 ul of rabbit anti-CD45recombinant monoclonal antibody (red shaded) or isotype control(unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP3-14668-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG .Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0004.jpg)
![Immunoprecipitation: CD45 Antibody [NBP3-14668] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from Jurkat cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-CD45recombinant monoclonal antibody used for IP at 20 ul/mg lysate. CD45 wasalso immunoprecipitated by a previous lot of this antibodyand rabbit anti-CD45 antibody. For blotting immunoprecipitated CD45,was used at 1:1000. Chemiluminescence withan exposure time of 250 milliseconds.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunoprecipitation-NBP3-14668-img0002.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser276] Antibody-Western Blot-NBP1-77807-img0010.jpg "Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.")
![Immunohistochemistry: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - Diluted to1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-NBP1-77807-img0007.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS529 shows phospho p65 staining in carcinoma cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0005.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS276 shows phospho p65 staining in carcinoma cells. Western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes and does not show significant levels of phosphorylated p65. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0006.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0008.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16589] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0007.jpg "Western Blot: Galectin-3 Antibody [NBP2-16589] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16589] - HBL435 xenograft, using Galectin3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16589-img0003.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16589] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16589] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0004.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16589] - Galectin-3 antibody detects Galectin-3 protein by western blot analysis. A. 50 ug rat colon lysate/extract. 12% SDS-PAGE. Galectin-3 antibody dilution: 1:10000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0006.jpg)
![Sandwich ELISA: Galectin-3 Antibody [NBP2-16589] - Sandwich ELISA detection of recombinant full-length Galectin-3 protein using as capture antibody at concentration of 5 ug/mL and Galectin-3 Antibody as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Sandwich-ELISA-NBP2-16589-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16589] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16589_rabbit-polyclonal-galectin-3-antibody-1610202419414598.jpg)
![Western Blot: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Western-Blot-NBP1-48040-img0022.jpg "Western Blot: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.")
![Immunocytochemistry/Immunofluorescence: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TYRO3.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunocytochemistry-Immunofluorescence-NBP1-48040-img0013.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Human Ovary tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0021.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0014.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0015.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0016.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0017.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Human breast tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0018.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Human Kidney tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0019.jpg)
![Immunohistochemistry-Paraffin: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Staining of paraffin-embedded Human lung tissue using anti-TYRO3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunohistochemistry-Paraffin-NBP1-48040-img0020.jpg)
![Immunoprecipitation: Dtk/TYRO3 Antibody (2C4) [NBP1-48040] - Lane 1: Negative Control Lane 2: NBP1-48039 Lane 3: NBP1-48040 Lane 4: NBP1-48041 Lane 5: NBP1-48042 Lane 6: NBP1-48043 Lane 7: NBP1-48044 Lane 8: NBP1-48045](http://images.novusbio.com/fullsize/Dtk-TYRO3-Antibody-2C4-Immunoprecipitation-NBP1-48040-img0023.jpg)
![Western Blot: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipoamide Dehydrogenase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Lipoamide Dehydrogenase.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Western-Blot-NBP2-01109-img0002.jpg "Western Blot: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipoamide Dehydrogenase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Lipoamide Dehydrogenase.")
![Immunocytochemistry/Immunofluorescence: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Lipoamide Dehydrogenase.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Immunocytochemistry-Immunofluorescence-NBP2-01109-img0007.jpg)
![Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Human pancreas tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Immunohistochemistry-Paraffin-NBP2-01109-img0006.jpg)
![Flow Cytometry: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Lipoamide Dehydrogenase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Flow-Cytometry-NBP2-01109-img0001.jpg)
![Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Immunohistochemistry-Paraffin-NBP2-01109-img0003.jpg)
![Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Human endometrium tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Immunohistochemistry-Paraffin-NBP2-01109-img0004.jpg)
![Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Human lymph node tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Immunohistochemistry-Paraffin-NBP2-01109-img0005.jpg)