| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-70342-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22116SS | |||
| Azide and BSA Free | NBP2-80689 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74571SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-82375SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88697-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78538SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| Azide and BSA Free | NBP2-80831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44421 | |||
| BSA Free | NBP2-42388-25ul | |||
![Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Western Blot analysis of Human thyroid lysate showing detection of SLC5A5/Sodium Iodide Symporter protein using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-FP5-Western-Blot-NBP1-70342-img0007.jpg "Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Western Blot analysis of Human thyroid lysate showing detection of SLC5A5/Sodium Iodide Symporter protein using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000.")
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-FP5-Immunohistochemistry-NBP1-70342-img0010.jpg)
![Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Effects of 10 uM resveratrol on NIS protein expression. FRTL-5 cells were grown to 60% confluency in 6H5% medium, and then shifted to 5H5% medium for 6 days, before being cultured again in 6H5% medium for 24 h, and finally treated with resveratrol for 48 h. A representative Western blot is shown (top), with quantification (bottom). Data are the normalized means +/-SD (against actin) from three independent experiments (control vehicle: 100%). Control, cells treated with the control vehicle (0.5% ethanol); Resv, cells treated with 10 uM resveratrol. *, p<0.05 versus relevant control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0107936), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-FP5-Western-Blot-NBP1-70342-img0006.jpg)
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-FP5-Immunohistochemistry-NBP1-70342-img0008.jpg)
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-FP5-Immunohistochemistry-NBP1-70342-img0009.jpg)
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-FP5-Immunohistochemistry-NBP1-70342-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Staining of a 9 days old zebrafish embryo](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunocytochemistry-Immunofluorescence-NBP1-97712-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Staining of a 9 days old zebrafish embryo")
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Indirect immunoperoxidase staining of human colon adenocarcinoma paraffin tissue section with NBP1-97712 (RCK108; Mouse anti keratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial tumor cells. No reactivity in connective tissues.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunohistochemistry-Paraffin-NBP1-97712-img0020.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Flow Cytometry: Cytokeratin 19 Antibody (RCK108) [DyLight 650] [NBP1-97712C] - An intracellular stain was performed on HT-29 cells with Cytokeratin 19 (RCK108) NBP1-97712C (blue) and a matched isotype control NBP1-97005C (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight650. Image using the DyLight 650 form of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Flow-Cytometry-NBP1-97712-img0014.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Analysis using the Unpurified version of NBP1-97712. Immunohistochemistry on frozen section of human liver bile duct epithelium](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunohistochemistry-Frozen-NBP1-97712-img0010.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Immunohistochemistry on a frozen tissue sections of human kidney with NBP1-97712 (RCK108; dilution 1:500).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunohistochemistry-Frozen-NBP1-97712-img0015.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Immunohistochemistry on frozen tissue sections of human colon with NBP1-97712 (RCK108; dilution 1:500).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunohistochemistry-Frozen-NBP1-97712-img0017.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Indirect immunoperoxidase staining of human small intestine paraffin tissue section with NBP1-97712 (RCK108; Mouse anti Cytokeratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunohistochemistry-Paraffin-NBP1-97712-img0018.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Indirect immunoperoxidase staining of human placenta paraffin tissue section with NBP1-97712 (RCK108; Mouse anti Cytokeratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunohistochemistry-Paraffin-NBP1-97712-img0019.jpg)
![Western Blot: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Western blot analysis using Cytokeratin 19 mouse mAb against T47D (1), MCF-7 (2), HepG2 (3) and SW620 (4) cell lysate.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Western-Blot-NBP2-22116-img0003.jpg "Western Blot: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Western blot analysis using Cytokeratin 19 mouse mAb against T47D (1), MCF-7 (2), HepG2 (3) and SW620 (4) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunofluorescence analysis of HepG2 cells using Cytokeratin 19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Immunocytochemistry-Immunofluorescence-NBP2-22116-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Immunohistochemistry-Paraffin-NBP2-22116-img0007.jpg)
![Western Blot: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Western blot analysis using Cytokeratin 19 mAb against human Cytokeratin 19 (AA: 115-269) recombinant protein. (Expected MW is 43.1 kDa)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Western-Blot-NBP2-22116-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Immunohistochemistry-Paraffin-NBP2-22116-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Immunohistochemistry-Paraffin-NBP2-22116-img0006.jpg)
![ELISA: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-ELISA-NBP2-22116-img0001.jpg)
![Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NB110-74571-img0004.jpg "Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.")
![Immunocytochemistry/Immunofluorescence: Striatin Antibody [NB110-74571] - Immunofluorescent staining of NIH3T3 cells ± blocking peptide using NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74571-img0003.jpg)
![Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Western-Blot-NB110-82375-img0005.jpg "Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Simple Western: Mucolipin 1 Antibody [NB110-82375] - Lane view shows a specific band for Mucolipin 1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Simple-Western-NB110-82375-img0004.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - S1P inhibition induces autophagy and affects multiple UPR-related TFs. In vitro day 3 B-cells were treated with 10 uM PF-429242 or vehicle control (dH2O) for 72 h and were assessed for cell number using CountBright beads. Shown are the average cell numbers from 7 donors +/- standard deviations. Protein lysates generated from day 3 cells treated for 72 h with 10 uM PF-429242 were evaluated for CREB3L2 and ATF6 by Western blotting. Shown are representative results from 2 donors, a total of 7 donors were evaluated. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-32705-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP1-88697-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: CREB3L2 Antibody [NBP1-88697] - S1P inhibition induces autophagy and affects multiple UPR-related TFs. In vitro day 3 B-cells were treated with 10 uM PF-429242 or vehicle control (dH2O) for 72 h and were assessed for cell number using CountBright beads. Shown are the average cell numbers from 7 donors +/- standard deviations. Protein lysates generated from day 3 cells treated for 72 h with 10 uM PF-429242 were evaluated for CREB3L2 and ATF6 by Western blotting. Shown are representative results from 2 donors, a total of 7 donors were evaluated. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-32705-7), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CREB3L2 Antibody [NBP1-88697] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-88697-img0011.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - Analysis in control (vector only transfected HEK293T lysate) and CREB3L2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP1-88697-img0006.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - Expression of CREB3L2 in differentiating primary human B-cells. Protein expression of CREB3L2 during in vitro differentiation of primary human B-cells. The day of culture is indicated at the top. Intact and dashed arrows show migration of full-length protein and cleaved C-terminus, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-32705-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Western-Blot-NBP1-88697-img0007.jpg)
![Immunohistochemistry-Paraffin: CREB3L2 Antibody [NBP1-88697] - Staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-88697-img0003.jpg)
![Immunohistochemistry-Paraffin: CREB3L2 Antibody [NBP1-88697] - Staining of human kidney shows strong cytoplasmci positivity in cells in tubules.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-88697-img0009.jpg)
![Immunohistochemistry-Paraffin: CREB3L2 Antibody [NBP1-88697] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CREB3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-88697-img0010.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - S1P inhibition induces autophagy & affects multiple UPR-related TFs. (A) In vitro day 3 B-cells were treated with 10 μM PF-429242 or vehicle control (dH2O) for 72 h & were assessed for cell number using CountBright beads. Shown are the average cell numbers from 7 donors ± standard deviations. Protein lysates generated from day 3 cells treated for 72 h with 10 μM PF-429242 were evaluated for (B) autophagy markers or (C) CREB3L2 & ATF6 by Western blotting. Shown are representative results from 2 donors, a total of 7 donors were evaluated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30254311), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88697_rabbit-polyclonal-creb3l2-antibody-310202415304281.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - Kinetic & dose analysis of S1P-specific inhibitor PF-429242 during the early phase of ASC generation. (A) In vitro activated human B-cells were treated with 10 μM PF-429242 or vehicle control (dH2O) at day 3 & assessed for CREB3L2 processing at 12 h intervals. Representative results are shown from a total of 7 donors. (B) Samples from part (A) were assessed for cell number at the indicated time points. Displayed are the average cell number ± standard deviation derived from 7 donors. (C) In vitro activated human B-cells from 4 donors were treated with indicated amount PF-429242 or vehicle control (dH2O) at day 3 & assessed for cell number at 48 h. Results are displayed as the percentage viable cells relative to control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30254311), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88697_rabbit-polyclonal-creb3l2-antibody-310202415382439.jpg)
![Western Blot: CREB3L2 Antibody [NBP1-88697] - S1P-specific inhibitor PF-429242 blocks CREB3L2 processing. (A) Diagram of CREB3L2 structure with proteolytic cleavage sites. Act domain, Activation Domain. bZIP, Basic Leucine Zipper Domain. TM, Transmembrane Domain. (B) In vitro generated human ASCs were treated with 10 μM PF-429242 or vehicle control (dH2O) on the day that appears first (d6, d8, d10) & protein lysates generated on the day that appears subsequently (d8, d10, d13) were analyzed by Western blotting. Intact & dashed arrows show migration of CREB3L2 full-length protein & cleaved C-terminus, respectively. (C) In vitro differentiated human B-cells were treated with 10 μM PF-429242 or vehicle control (dH2O) at day 6 & analyzed subsequently for phenotype on the indicated days. Live cells were evaluated for expression of B-cell markers CD19 & CD20 & ASC markers CD38 & CD138. Percentages of cells are shown within the quadrant gates. The experiment was performed with 4 donors, a representative donor is shown. (D) Samples treated as in part (B) were assessed for cell number using CountBright beads. (E) In vitro activated human B-cells from 4 donors were treated with indicated amount PF-429242 or vehicle control (dH2O) at day 6 & assessed for cell number at 48 h. Results are displayed as the percentage viable cells relative to control. Supernatants from samples in part (B) were analyzed by ELISA for production of (F) IgM & (G) IgG. Significance was determined by t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30254311), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88697_rabbit-polyclonal-creb3l2-antibody-310202416121935.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0011.jpg "Western Blot: Staufen Antibody [NBP1-33202] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Staufen Antibody [NBP1-33202] - Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Staufen stained by Staufen antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33202-img0008.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Sample (30 ug of whole cell lysate) A: Neuro2A diluted at 1:1000](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0005.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Staufen antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0010.jpg)
![Immunoprecipitation: Staufen Antibody [NBP1-33202] - Staufen antibody staining 30 ug whole cell lysate of Lane A: 293 (input), Lane B: control rabbit IgG-IP, Lane C: Stau1-IP, Lane D: Post-IP lysate from control rabbit IgG-IP, Lane E:Post-IP lysate from Stau1-IP; diluted at 1:3000.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunoprecipitation-NBP1-33202-img0006.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Silencing of STAU1 mitigates SCA2 phenotypes. aStau1 haploinsufficiency improves abnormal motor behavior of ATXN2Q127 mice as determined by rotarod behavior at 8, 12, 16, & 20 weeks of age. ATXN2Q127;Stau1+/- mice (green) have improved rotarod performance compared with ATXN2Q127 littermates (red) starting at 12 weeks of age. Note that Stau1 haploinsufficiency (orange) by itself does not alter motor function; n = 9–15 mice per group. Values shown are mean ± SE. Significance was determined using generalized estimating equations (GEE). NS, nonsignificant, *P  4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P  4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P  4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Staufen stained by Staufen antibody (NBP1-33202) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33202_rabbit-polyclonal-staufen-antibody-immunocytochemistry-immunofluorescence-26112025185041.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Metabolic alterations in the MPC1-/- cells expressed significantly higher levels glutaminolysis related proteins GDH (Glutamate Dehydrogenase) and GLS (NBP2-29940), but there was no significant change in the PDHE1 protein expression in these cells as shown in representative western blots. The alpha-Tublin was used as loading control. ***PSample: Paraffin-embedded rat liver.Glutamate Dehydrogenase stained by GLUD1 + GLUD2 antibody (NBP2-16679) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16679_rabbit-polyclonal-glutamate-dehydrogenase-antibody-immunohistochemistry-paraffin-261120251981014.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Metabolic alterations in the MPC1-/- cells expressed significantly higher levels glutaminolysis related proteins GDH (Glutamate Dehydrogenase) and GLS (NBP2-29940), but there was no significant change in the PDHE1 protein expression in these cells as shown in representative western blots. The alpha-Tublin was used as loading control. ***PSample: Paraffin-embedded rat liver.Glutamate Dehydrogenase stained by GLUD1 + GLUD2 antibody (NBP2-16679) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Laser scanning confocal microscopy was carried out using human tissue sections from frontal cortex. Figure A shows an example section that was immunostained for the presence of pyrE3Abeta (green). Figure B shows that the immunogen peptide completely blocked the NBP1-44048 antibody.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu3]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44048-img0005.jpg "Immunocytochemistry/Immunofluorescence: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Laser scanning confocal microscopy was carried out using human tissue sections from frontal cortex. Figure A shows an example section that was immunostained for the presence of pyrE3Abeta (green). Figure B shows that the immunogen peptide completely blocked the NBP1-44048 antibody.")
![Western Blot: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Lane 1-10: beta Amyloid pyro E3 peptide. Load: 1 ug per lane 1-2, 0.2 ug per lane 3-4, 0.05 ug per lane 5-6, 0.01 ug per lane 7-8, 0.005 ug per lane 9-10. Primary antibody: Beta Amyloid pyro E3 antibody at 0.5 ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide. Cross reactivity with Beta Amyloid 3 peptide is >1000 fold below pyrE3 reactivity and contains no reactivity towards the Beta Amyloid 42 A beta peptide (data not shown).](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu3]-Antibody-Western-Blot-NBP1-44048-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Tissue: human brain section. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: beta Amyloid pyro E3 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: beta Amyloid pyro E3 is nuclear and cytoplasmic. Staining: Top: Beta Amyloid pyro E3 as as green fluorescent signal, Beta Amyloid 3 as yellow signal; and Bottom: Beta Amyloid 3 as red signal with co-incubation of Beta Amyloid pyro E3 peptide.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu3]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44048-img0007.jpg)
![Western Blot: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Lane 1: beta Amyloid 3 peptide Lane 2: beta Amyloid pyro E3 peptide. Load: 1 ug per lane. Primary antibody: Beta Amyloid pyro E3 antibody at 2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu3]-Antibody-Western-Blot-NBP1-44048-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: PIEZO2 Antibody [NBP1-78538] - PIEZO2 antibody was tested at 1:100 in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PIEZO2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78538-img0005.jpg "Immunocytochemistry/Immunofluorescence: PIEZO2 Antibody [NBP1-78538] - PIEZO2 antibody was tested at 1:100 in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry: PIEZO2 Antibody [NBP1-78538] - Analysis of PIEZ02 in mouse epidermis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PIEZO2-Antibody-Immunohistochemistry-NBP1-78538-img0006.jpg)
![Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Knockout-Validated-NBP2-60735-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Simple Western: LC3B Antibody (1251B) [NBP2-60735] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Simple-Western-NBP2-60735-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunocytochemistry-Immunofluorescence-NBP2-60735-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Western-Blot-NBP2-60735-img0010.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251B) [NBP2-60735] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells. A few cells depicted LC3 puncta formation/dotted staining in apparently autophagic cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunohistochemistry-Paraffin-NBP2-60735-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hours. An intracellular stain was performed with anti-LC3B (1251B) antibody [Catalog # NBP2-60735] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Flow-Intracellular-NBP2-60735-img0009.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining in human fallopian tube and liver tissues. Corresponding LAMC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Immunohistochemistry-Paraffin-NBP2-42388-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining in human fallopian tube and liver tissues. Corresponding LAMC2 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMC2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Western-Blot-NBP2-42388-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of A-431 cells using the Anti-LAMC2 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Immunocytochemistry-Immunofluorescence-NBP2-42388-img0025.jpg)
![Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Western-Blot-NBP2-42388-img0015.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human skin shows moderate positivity in basement membrane of epidermis.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Immunohistochemistry-Paraffin-NBP2-42388-img0018.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human fallopian tube shows moderate positivity in basement membrane of glandular cells.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Immunohistochemistry-Paraffin-NBP2-42388-img0019.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human small intestine shows moderate positivity in basement membrane of glandular cells.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Immunohistochemistry-Paraffin-NBP2-42388-img0020.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Immunohistochemistry-Paraffin-NBP2-42388-img0021.jpg)