| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92695SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73042 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-31123SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-178SS | |||
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Human breast carcinoma.Tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1183-img0010.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Human breast carcinoma.Tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with breast carcinoma.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1183-img0001.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with human, pancreas, islet.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1183-img0003.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with human adrenal.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1183-img0004.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Human Skin, Melanoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1183-img0005.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Anti-GPER1 / GPR30 antibody IHC of human colon, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1183-img0006.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Anti-GPER1 / GPR30 antibody IHC of human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1183-img0007.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Brain, Parkinson's Lewy Body](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1183-img0008.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Brain, cerebellum, Purkinje neuron](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NLS1183-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (RV202) [NBP1-97672] - Staining of a 1 month old zebrafish embryo](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV202-Immunocytochemistry-Immunofluorescence-NBP1-97672-img0006.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (RV202) [NBP1-97672] - Staining of a 1 month old zebrafish embryo")
![Immunohistochemistry-Frozen: Vimentin Antibody (RV202) [NBP1-97672] - Immunohistochemistry on frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV202-Immunohistochemistry-Frozen-NBP1-97672-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (RV202) [NBP1-97672] - Staining of the developing neural tube in a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV202-Immunocytochemistry-Immunofluorescence-NBP1-97672-img0005.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV202) [NBP1-97672] - MUB1900L1: Frozen section of swine colon immunostained with RV202-FITC (1:200)](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV202-Immunohistochemistry-Frozen-NBP1-97672-img0002.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV202) [NBP1-97672] - Frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV202-Immunohistochemistry-Frozen-NBP1-97672-img0001.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV202) [NBP1-97672] - Frozen section of swine colon immunostained with RV202-HRP (1:200)](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV202-Immunohistochemistry-Frozen-NBP1-97672-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV202) [NBP1-97672] - Paraffin section of human colon](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV202-Immunohistochemistry-Paraffin-NBP1-97672-img0004.jpg)
![Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Western-Blot-NBP1-92695-img0005.jpg "Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.")
![Immunocytochemistry/Immunofluorescence: TARDBP Antibody (3H8) [NBP1-92695] - NBP1-92695 was used to stain a section of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Chicken antibody to GFAP (NBP1-05198, green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TARDBP (3H8) antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TARDBP and blue due to their content of DNA, stained blue with DAPI.](http://images.novusbio.com/fullsize/TARDBP-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP1-92695-img0003.jpg)
![Immunohistochemistry Free-Floating: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of rat hippocampus section stained with mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in red, and costained with chicken pAb to GFAP,, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The TDP43 protein is concentrated in the nuclei of hippocampal neurons, while the GFAP antibody stains the intermediate filament network of astroglial cells.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP1-92695-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Lysates of HAP1 (WT) and TDP-43/TARDBP (KO) were prepared, and 50 ug of protein was processed for western blot with the TDP-43/TARDBP antibody. The Ponceau stained transfers of the blot is shown (right). Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Knockout-Validated-NBP1-92695-img0006.jpg)
![Immunoprecipitation: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of the TDP-43/TARDBP antibody pre-coupled to Dynabeads protein G. Sample was washed and processed for immunoblot with the TDP-43/TARDBP antibody. The Ponceau stained transfer of the blot is shown. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Immunoprecipitation-NBP1-92695-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 (WT) and TDP-43/TARDBP (KO) cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with glass bottom. Cells were stained with the TDP-43/TARDBP antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined on both channels with green and magenta dashed line, respectively. Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Knockout-Validated-NBP1-92695-img0008.jpg)
![Western Blot: FUS Antibody [NB100-562] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FUS-Antibody-Knockout-Validated-NB100-562-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody [NB100-562] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody [NB100-562] - Detection of human FUS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FUS antibody NB100-562 (lot NB100-562-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FUS was also immunoprecipitated by a previous lot (lot NB100-562-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NB100-562-img0010.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-562] - Section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NB100-562-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: FUS Antibody [NB100-562] - Detection of human FUS on HeLa whole cell lysate using NB100-562. For IP in B, rabbit anti-FUS antibodies NB100-560, NB100-561, NB100-562 and NB100-565 were used.](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NB100-562-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16908-img0041.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI .")
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: AIF-1:Iba1 Antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-16908-img0032.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0035.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0036.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - Primary murine microglia cells, staining with AIF-1:Iba1 Antibody using 1. 0 ug per 4 x 10^5 cells. NBP2-16908 (blue), Rabbit IgG (green) Unstained (red).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-16908-img0014.jpg)
![Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 antibody (NBP2-16908) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16908_rabbit-polyclonal-aif-1-iba1-antibody-910202419593573.jpg)
![Western Blot: Notch-1 Antibody (3E12) [NBP1-48289] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Western-Blot-NBP1-48289-img0030.jpg "Western Blot: Notch-1 Antibody (3E12) [NBP1-48289] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1.")
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of HepG2 cells using anti Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunocytochemistry-Immunofluorescence-NBP1-48289-img0032.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded thyroid using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunocytochemistry-Immunofluorescence-NBP1-48289-img0016.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0017.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0018.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded breast using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0019.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0020.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of lung using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0021.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0022.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0023.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded colon using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0024.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded endometrium using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0025.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded kidney using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0026.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded lung using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0027.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded pancreas using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0028.jpg)
![Western Blot: ITPR2 Antibody [NB100-2466] - 2 sec exposure using NB100-2466 on 80 ug total cell lysates listed above. Primary antibody applied at 1:500 overnight at 4degC, secondary donkey anti-goat applied for 2 hours at room temperature at 1:5000. (Data provided by Karen McColl at Case Western Reserve University)](http://images.novusbio.com/fullsize/ITPR2-Antibody-Western-Blot-NB100-2466-img0001.jpg "Western Blot: ITPR2 Antibody [NB100-2466] - 2 sec exposure using NB100-2466 on 80 ug total cell lysates listed above. Primary antibody applied at 1:500 overnight at 4degC, secondary donkey anti-goat applied for 2 hours at room temperature at 1:5000. (Data provided by Karen McColl at Case Western Reserve University)")
![Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ITPR2-Antibody-Immunohistochemistry-Paraffin-NB100-2466-img0004.jpg)
![Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ITPR2-Antibody-Immunohistochemistry-Paraffin-NB100-2466-img0002.jpg)
![Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ITPR2-Antibody-Immunohistochemistry-Paraffin-NB100-2466-img0003.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Western-Blot-NB100-1194-img0007.jpg "Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1194_goat-polyclonal-park7-dj-1-antibody-131120231145588.jpg)
![Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody [NB100-1194] - Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml NB100-1194 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.](http://images.novusbio.com/fullsize/nb100-1194_goat-polyclonal-park7-dj-1-antibody-1311202311455816.jpg)
![Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] - (7ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-1194_goat-polyclonal-park7-dj-1-antibody-131120231145587.jpg)
![Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-1194_goat-polyclonal-park7-dj-1-antibody-131120231145584.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - A. 30 ug HepG2 whole cell lysate/extract 12% SDS-PAGE Apolipoprotein E antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Western-Blot-NBP1-31123-img0032.jpg "Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - A. 30 ug HepG2 whole cell lysate/extract 12% SDS-PAGE Apolipoprotein E antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody [NBP1-31123] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31123-img0033.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Apolipoprotein E antibody [C2C3], C-term detects secreted Apolipoprotein E protein by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (NBP1-31123).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP1-31123-img0036.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cells B : Control with 3ug of pre-immune rabbit IgG C : Immunoprecipitation of Apolipoprotein E by 3 ug of Apolipoprotein E antibody [C2C3], C-term 10% SDS-PAGE The immunoprecipitated Apolipoprotein E protein was detected by Apolipoprotein E antibody [C2C3], C-term diluted at 1 : 1000. Easy Blot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Western-Blot-NBP1-31123-img0006.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term diluted at 1:5000.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Western-Blot-NBP1-31123-img0007.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Sample (2. 5 ug of whole cell lysate) A: Conditional medium from human primary preadipocyte B: Conditional medium from differentiated human primary preadipocyte 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Western-Blot-NBP1-31123-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by analysis. Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min. Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31123-img0008.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Human hepatoma. Apolipoprotein E antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP1-31123-img0026.jpg)
![ELISA: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Sandwich ELISA detection of recombinant full-length Apolipoprotein E protein using as capture antibody at concentration of 5 ug/mL and a mouse monoclonal anti-Apolipoprotein E antibody as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-ELISA-NBP1-31123-img0035.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (812) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Western-Blot-NBP2-43560-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (812) [NBP2-43560] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (812) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Frozen sectioned E13.5 Rat brain. Green: Vimentin protein stained by Vimentin antibody [812] diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Immunohistochemistry-Frozen-NBP2-43560-img0006.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Analysis of Frozen section of embryonic mouse brain (mE18.5). Red: Vimentin antibody [812] diluted at 1:250. Blue: DAPI.](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Immunohistochemistry-Frozen-NBP2-43560-img0001.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract 10 % SDS-PAGE Vimentin antibody [812] dilution: 1:1000](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Western-Blot-NBP2-43560-img0002.jpg)
![Sandwich ELISA: Vimentin Antibody (812) [NBP2-43560] - Sandwich ELISA detection of recombinant full-length Vimentin protein using Vimentin Antibody (812) as capture antibody at concentration of 5 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Sandwich-ELISA-NBP2-43560-img0011.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Non-transfected (-) and transfected (+) 293T whole cell extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-910202420131230.jpg)
![ELISA: Vimentin Antibody (812) [NBP2-43560] - Sandwich ELISA detection of recombinant full-length Vimentin protein using NBP2-43560 as capture antibody at concentration of 5 ug/mL and detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-91020242041256.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP2-43560) diluted at 1:500000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-910202420131251.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:3000.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-western-blot-2611202519369.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-western-blot-2611202518434512.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-western-blot-261120251820010.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:75000.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-western-blot-261120251837248.jpg)
![Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.](http://images.novusbio.com/fullsize/NUMB-Antibody-Western-Blot-NB500-178-img0005.jpg "Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.")
![Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: NUMB Antibody [NB500-178] - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Numb Antibody (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0007.jpg)
![Simple Western: Numb Antibody [NB500-178] - Simple Western lane view shows a specific band for NUMB in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Numb-Antibody-Simple-Western-NB500-178-img0006.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - Detection of mouse and rat GAPDH by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GAPDH antibody NB300-325 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-325-img0006.jpg "Western Blot: GAPDH Antibody [NB300-325] - Detection of mouse and rat GAPDH by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GAPDH antibody NB300-325 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-325] - GAPDH antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NB300-325-img0003.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - Whole cell lysate (50 ug) from HeLa (H) and 293T (50 ug) probed with GAPDH Antibody diluted at 0.04 ug/ml](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-325-img0001.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - Detection of Mouse GAPDH Whole cell lysate (5, 15 and 50 ug) from mouse NIH3T3 cells. Another Affinity purified rabbit anti-GAPDH used at 0.04 mcg/ml. Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-325-img0005.jpg)
![Simple Western: GAPDH Antibody [NB300-325] - Simple Western lane view shows a specific band for GAPDH in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-325-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-310202416212345.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-310202416212378.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-310202416205140.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-31020241621812.jpg)