| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59770SS | |||
| Azide and BSA Free | NBP2-80894 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33168 | |||
| IHC-Prediluted | NBP2-44590 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33132 | |||
| IHC-Prediluted | NBP2-44711 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32932-0.1mg | |||
| BSA Free | NBP2-32932-0.02mg | |||
| Azide and BSA Free | NBP2-34713-0.2mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49529SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87914-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84830-25ul | |||
![Western Blot: OPA1 Antibody (1284B) [NBP2-59770] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Western-Blot-NBP2-59770-img0001.jpg "Western Blot: OPA1 Antibody (1284B) [NBP2-59770] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with OPA1 Antibody [1284B] conjugated to Biotin (NBP2-59770B) at 5 ug/ml for 1 hour at room temperature then detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunocytochemistry-Immunofluorescence-NBP2-59770-img0022.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody (1284B) [NBP2-59770] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse testes using OPA1 antibody at 1:1000 dilution. The primary antibody bound to OPA1 antigens in the tissue section was deteced using an HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This OPA1 antibody generated a diffused cytoplasmic staining of OPA1 protein in the tubular epithelial cells, the spermatids/spermatocytes and Leydig's cells.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunohistochemistry-Paraffin-NBP2-59770-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-OPA1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunocytochemistry-Immunofluorescence-NBP2-59770-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - The effect of N-BLR knockdown on invasion by specific siRNAs. E-cadherin, vimentin, and ZEB1 were identified in vitro by immunofluorescence with specific antibodies. Immunofluorescence signal of E-cadherin (green color) was markedly increased in both clones. The ZEB1 signal was present in cells with empty vector (green color) but not in clones #3-1 and #4-7. Blue color indicate nuclei. Single green, blue, and merged channel images of ZEB1 are reported in Additional file 3: Figure S9B. Image collected and cropped by CiteAb from the following publication (//genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1224-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Vimentin-Antibody-V9-Immunocytochemistry-Immunofluorescence-NBP1-97670-img0006.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - The effect of N-BLR knockdown on invasion by specific siRNAs. E-cadherin, vimentin, and ZEB1 were identified in vitro by immunofluorescence with specific antibodies. Immunofluorescence signal of E-cadherin (green color) was markedly increased in both clones. The ZEB1 signal was present in cells with empty vector (green color) but not in clones #3-1 and #4-7. Blue color indicate nuclei. Single green, blue, and merged channel images of ZEB1 are reported in Additional file 3: Figure S9B. Image collected and cropped by CiteAb from the following publication (//genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1224-0) licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of connective tissue cells in a frozen tissue section of human colon. No reactivity in epithelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-V9-Immunohistochemistry-Frozen-NBP1-97670-img0005.jpg)
![Flow Cytometry: Vimentin Antibody (V9) [NBP1-97670] - An intracellular stain was performed on Jurkat cells with Vimentin antibody (V9) NBP1-97670PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Vimentin-Antibody-V9-Flow-Cytometry-NBP1-97670-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of glomeruli and connetive tissue in human kidney with in a 1:200 dilution. No reactivity in epithelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-V9-Immunocytochemistry-Immunofluorescence-NBP1-97670-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of connective tissue cells in swine colon with in a 1:200 dilution. No reactivity in epithelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-V9-Immunocytochemistry-Immunofluorescence-NBP1-97670-img0004.jpg)
![Flow Cytometry: Vimentin Antibody (V9) [NBP1-97670] - Analysis of PE conjugate of NBP1-97670. An intracellular stain was performed on THP-1 cells with Vimentin antibody (V9) NBP1-97670PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% s](http://images.novusbio.com/fullsize/Vimentin-Antibody-V9-Flow-Cytometry-NBP1-97670-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in human tonsil. NBP1-97670 was diluted to 0.5ug/mL and was left on sections for 30m at room temperature. Secondary antibody was Horse-Anti Mouse HRP Veterinary Reagent. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-97670_mouse-monoclonal-vimentin-antibody-v9-immunohistochemistry-paraffin-1462023111714..jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in horse intestine. NBP1-97670 was used at a concentration of 0.5ug/mL and was left on slides for 30m at room temperature. Secondary antibody was Horse Anti-Mouse HRP Veterinary Reagent. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-97670_mouse-monoclonal-vimentin-antibody-v9-immunohistochemistry-paraffin-1462023112025..jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in feline intestine. NBP1-97670 was used at a concentration of 0.5ug/mL. Secondary antibody was Horse Anti-Mouse HRP Veterinary Reagent. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-97670_mouse-monoclonal-vimentin-antibody-v9-immunohistochemistry-paraffin-1462023112626.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in canine intestine. NBP1-97670 was used at a concentration of 0.5ug/mL and was left on tissue sections for 30m at room temperature. Secondary was Horse Anti-Mouse Veterinary Reagent. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-97670_mouse-monoclonal-vimentin-antibody-v9-immunohistochemistry-paraffin-1462023115136..jpg)
![Immunohistochemistry-Paraffin: Mouse Monoclonal Vimentin Antibody (V9) [NBP1-97670] - Vimentin was stained in human FFPE tonsil tissue. HIER antigen retrieval at pH 9 for 20min was performed. Concentration used: 1:100. AF750 conjugated version of the antibody was used (Catalog # NBP1-97670AF750). Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-97670_mouse-monoclonal-vimentin-antibody-v9-immunohistochemistry-paraffin-1122023155957..jpg)
![Western Blot: S100B Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Western-Blot-NBP2-29403-img0001.jpg "Western Blot: S100B Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9) [NBP2-29403] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunocytochemistry-Immunofluorescence-NBP2-29403-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - CODEX multiplexing image of S100b in primary melanoma of the skin. Image from verified customer review.](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0009.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100B antibody(4C4.9).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Monoclonal antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0007.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Antibody (4C4.9)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0008.jpg)
![Western Blot: FUS Antibody [NB100-561] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-FUS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 seconds.](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NB100-561-img0023.jpg "Western Blot: FUS Antibody [NB100-561] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-FUS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 seconds.")
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-561] - Detection of mouse FUS by immunohistochemistry. Sample: FFPE section of mouse teratoma Antibody: Affinity purified rabbit anti-FUS (NB100-561) used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NB100-561-img0024.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-561] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS (NB100-561). Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-NB100-561-img0020.jpg)
![Immunoprecipitation: FUS Antibody [NB100-561] - FUS immunoprecipitation in human fibroblast cell lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunoprecipitation-NB100-561-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: FUS Antibody [NB100-561] - Detection of human FUS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FUS antibody NB100-561 used for IP at 3 ug per reaction. FUS was also immunoprecipitated by rabbit anti-FUS recombinant monoclonal antibody [BLR023E] (NBP2-76415) and rabbit anti-FUS antibody NB100-565. For blotting immunoprecipitated FUS, NBP2-76415 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunoprecipitation-NB100-561-img0021.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis of NCAM-1/CD56 antibody (123C3.D5) on Human colon tissue. Incubation: 1 ug/ml for 30 minutes at room temperature. Image from verified customer review.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Immunohistochemistry-Paraffin-NBP2-15184-img0019.jpg "Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis of NCAM-1/CD56 antibody (123C3.D5) on Human colon tissue. Incubation: 1 ug/ml for 30 minutes at room temperature. Image from verified customer review.")
![Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Flow cytometry of lymphocyte gated PBMCs unstained (gray) orstained with CF568-labeled NCAM-1/CD56 antibody (123C3.D5) (orange).](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Flow-Cytometry-NBP2-15184-img0020.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Formalin-fixed, paraffin-embedded human colon Ganglion stained with CD56 Monoclonal Antibody (123C3.D5)](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Immunohistochemistry-Paraffin-NBP2-15184-img0016.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis using the Azide and BSA Free version of NBP2-15184. Staining of AF700 conjugated CD56 in human PBMC using anti-CD56 antibody. The primary antibody was used at a dilution of 1:100, incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Flow-Cytometry-NBP2-15184-img0014.jpg)
![Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Simple Western lane view shows a specific band for NCAM-1/CD56 in 0.2 mg/ml of IMR-32 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Simple-Western-NBP2-15184-img0007.jpg)
![Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Electropherogram image of the corresponding Simple Western lane. NCAM-1/CD56 antibody was used at 10 ug/ml dilution of IMR-32 lysates(s) respectively.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Simple-Western-NBP2-15184-img0012.jpg)
![Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Western-Blot-NBP1-32080-img0023.jpg "Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody [NBP1-32080] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32080-img0019.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Mouse brain. C3 antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-32080-img0015.jpg)
![Immunoprecipitation: Complement C3 Antibody [NBP1-32080] - Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 ug of C3 antibody [C3], C-term.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunoprecipitation-NBP1-32080-img0017.jpg)
![Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Complement C3 antibody [C3], C-term (NBP1-32080) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32080_rabbit-polyclonal-complement-c3-antibody-western-blot-1411202518442224.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713] - Formalin-paraffin human tonsil (20X) stained with CD34 MAb (QBEnd/10). Image using the standard format of this product.](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34713-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713] - Formalin-paraffin human tonsil (20X) stained with CD34 MAb (QBEnd/10). Image using the standard format of this product.")
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713] - Human placental tissue. Antigen retrieval at pH 9. IF by Cy3 conjugate. Image from verified customer review.](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34713-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD34 MAb (QBEnd/10) Image using the standard format of this product.](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34713-img0002.jpg)
![Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of Parkin on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Western-Blot-NBP2-67017-img0007.jpg "Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of Parkin on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded rat brain tissue using anti-Parkin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunohistochemistry-Paraffin-NBP2-67017-img0004.jpg)
![Flow Cytometry: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of SH-SY-5Y cells with Parkin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Flow-Cytometry-NBP2-67017-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0006.jpg)
![Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded mouse testis tissue using anti-Parkin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunohistochemistry-Paraffin-NBP2-67017-img0003.jpg)
![Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] -Analysis of Parkin on different lysates with Rabbit anti-Parkin antibody at 1/1,000 dilution.Lane 1: 293 cell lysateLane 2: Jurkat cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 52 kDaObserved band size: 52 kDaExposure time: 1 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67017_rabbit-monoclonal-parkin-antibody-jf82-09-western-blot-1962024104114..jpg)
![Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.](http://images.novusbio.com/fullsize/ApoE4-Antibody-4E4-Western-Blot-NBP1-49529-img0004.jpg "Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.")
![Immunocytochemistry/Immunofluorescence: ApoE4 Antibody (4E4) [NBP1-49529] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE4 (4E4) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ApoE4-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-49529-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Mena Antibody [NBP1-87914] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and focal adhesion sites. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87914-img0015.jpg "Immunocytochemistry/Immunofluorescence: Mena Antibody [NBP1-87914] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and focal adhesion sites. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Analysis in human testis and liver tissues. Corresponding ENAH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87914-img0020.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human fallopian tube, liver, prostate and testis using Anti-ENAH antibody (A) NBP1-87914 shows similar protein distribution across tissues to independent antibody NBP1-87915 (B).](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87914-img0025.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human Fallopian tube shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87914-img0021.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human liver shows low positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87914-img0022.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human prostate shows moderate to strong cytoplasmic and membranous positivity in glandular cells and smooth muscle cells.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87914-img0023.jpg)
![Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Mena-Antibody-Immunohistochemistry-Paraffin-NBP1-87914-img0024.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Ephrin-B2 Antibody [NBP1-84830] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84830-img0003.jpg)
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human placenta shows moderate membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-84830-img0008.jpg)
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human colon shows weak to moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-84830-img0005.jpg)
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-84830-img0006.jpg)
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human lung shows weak to moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-84830-img0007.jpg)
![Western Blot: Ephrin-B2 Antibody [NBP1-84830] - Colonic levels of EphB4 & ephrin-B2 mRNA. (A) Representative agarose gels showing mRNA levels of EphB4, ephrin-B2 & GAPDH from the colon of vehicle-treated normal mice (N; n = 7) & of TNBS-treated mice administered with vehicle (CNT; n = 4) or EphB4 20 µg/kg (EphB; n = 5). Histograms represent the quantification of EphB4 (B) & ephrin-B2 (C) mRNA levels normalized to GAPDH amplification products. Transcript X1 (black bars) & transcript X2 (white bars), encoded by ephrin-B2 gene, were detected & represented (C). Data are shown as mean ± SEM. *P < 0.05 vs. N mice, one-way ANOVA followed by Bonferroni’s post-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31297055), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84830_rabbit-polyclonal-ephrin-b2-antibody-31020241539743.jpg)
