| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43802 | |||
| BSA Free | NBP2-46642-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32020-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89330-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83250-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-44270-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50050SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB600-939-0.1mg | |||
| HRP | NB120-7339-0.1mg | |||
![Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining in human kidney and fallopian tube tissues. Corresponding DDC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-CL2962-Immunohistochemistry-Paraffin-NBP2-46642-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining in human kidney and fallopian tube tissues. Corresponding DDC RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining of mouse substantia nigra shows strong positivity in dopamine neurons in pars compacta.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-CL2962-Immunohistochemistry-NBP2-46642-img0006.jpg)
![Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining of human substantia nigra shows cytoplasmic positivity in dopamine neurons in pars compacta.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-CL2962-Immunohistochemistry-Paraffin-NBP2-46642-img0007.jpg)
![Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-CL2962-Immunohistochemistry-Paraffin-NBP2-46642-img0008.jpg)
![Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining of human fallopian tube shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-CL2962-Immunohistochemistry-Paraffin-NBP2-46642-img0009.jpg)
![Immunohistochemistry: Dopa Decarboxylase/DDC Antibody (CL2962) [NBP2-46642] - Staining of rat substantia nigra shows strong positivity in dopamine neurons in pars compacta.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-CL2962-Immunohistochemistry-NBP2-46642-img0012.jpg)
![Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Analysis in human kidney and lymph node tissues. Corresponding SLC4A4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SLC4A4-Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Analysis in human kidney and lymph node tissues. Corresponding SLC4A4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining of human pancreas shows strong membranous positivity in intercalated ducts.](http://images.novusbio.com/fullsize/SLC4A4-Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0012.jpg)
![Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining of human kidney shows strong cytoplasmic/ membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/SLC4A4-Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0010.jpg)
![Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining of human lymph node shows no positivity as expected.](http://images.novusbio.com/fullsize/SLC4A4-Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0011.jpg)
![Immunohistochemistry-Paraffin: SLC4A4 Antibody [NBP2-32020] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/SLC4A4-Antibody-Immunohistochemistry-Paraffin-NBP2-32020-img0013.jpg)
![Immunohistochemistry-Paraffin: QPRT Antibody [NBP3-17089] - Analysis in human liver and skeletal muscle tissues using Anti-QPRT antibody. Corresponding QPRT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/QPRT-Antibody-Immunohistochemistry-Paraffin-NBP3-17089-img0002.jpg "Immunohistochemistry-Paraffin: QPRT Antibody [NBP3-17089] - Analysis in human liver and skeletal muscle tissues using Anti-QPRT antibody. Corresponding QPRT RNA-seq data are presented for the same tissues.")
![Western Blot: QPRT Antibody [NBP3-17089] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/QPRT-Antibody-Western-Blot-NBP3-17089-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: QPRT Antibody [NBP3-17089] - Staining of human cell line Hep G2 shows localization to vesicles.](http://images.novusbio.com/fullsize/QPRT-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17089-img0001.jpg)
![Immunohistochemistry-Paraffin: QPRT Antibody [NBP3-17089] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/QPRT-Antibody-Immunohistochemistry-Paraffin-NBP3-17089-img0004.jpg)
![Immunohistochemistry-Paraffin: QPRT Antibody [NBP3-17089] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/QPRT-Antibody-Immunohistochemistry-Paraffin-NBP3-17089-img0003.jpg)
![Western Blot: ASPA Antibody [NBP1-89258] - Analysis in control (vector only transfected HEK293T lysate) and ASPA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ASPA-Antibody-Western-Blot-NBP1-89258-img0007.jpg "Western Blot: ASPA Antibody [NBP1-89258] - Analysis in control (vector only transfected HEK293T lysate) and ASPA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-89258] - Analysis in human kidney and tonsil tissues using NBP1-89258 antibody. Corresponding ASPA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ASPA-Antibody-Immunohistochemistry-Paraffin-NBP1-89258-img0012.jpg)
![Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-89258] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/ASPA-Antibody-Immunohistochemistry-Paraffin-NBP1-89258-img0008.jpg)
![Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-89258] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ASPA-Antibody-Immunohistochemistry-Paraffin-NBP1-89258-img0009.jpg)
![Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-89258] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ASPA-Antibody-Immunohistochemistry-Paraffin-NBP1-89258-img0010.jpg)
![Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-89258] - Staining of human tonsil shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/ASPA-Antibody-Immunohistochemistry-Paraffin-NBP1-89258-img0011.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining in human kidney and lymph node tissues using anti-ATP6V0A4 antibody. Corresponding ATP6V0A4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining in human kidney and lymph node tissues using anti-ATP6V0A4 antibody. Corresponding ATP6V0A4 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human cerebral cortex, colon, kidney and liver using Anti-ATP6V0A4 antibody NBP1-89330 (A) shows similar protein distribution across tissues to independent antibody NBP2-39030 (B).](http://images.novusbio.com/fullsize/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0008.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human colon.](http://images.novusbio.com/fullsize/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0011.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0005.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0009.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human liver.](http://images.novusbio.com/fullsize/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0010.jpg)
![Immunohistochemistry-Paraffin: HFE Antibody [NBP1-83250] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/HFE-Antibody-Immunohistochemistry-Paraffin-NBP1-83250-img0010.jpg "Immunohistochemistry-Paraffin: HFE Antibody [NBP1-83250] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.")
![Immunohistochemistry: HFE Antibody [NBP1-83250] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/HFE-Antibody-Immunohistochemistry-NBP1-83250-img0008.jpg)
![Immunohistochemistry-Paraffin: HFE Antibody [NBP1-83250] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/HFE-Antibody-Immunohistochemistry-Paraffin-NBP1-83250-img0009.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Western-Blot-NB100-40805-img0004.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Frozen: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of PFA-perfused cryosection of Porcine Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Immunohistochemistry-Frozen-NB100-40805-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Immunohistochemistry-Paraffin-NB100-40805-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Immunohistochemistry-Paraffin-NB100-40805-img0002.jpg)
![Western Blot: MBP Antibody [NBP2-29656] - rat cortex homogenate showing specific immunolabeling of the ~ 18 & 22k MBP protein.](http://images.novusbio.com/fullsize/MBP-Antibody-Western-Blot-NBP2-29656-img0001.jpg "Western Blot: MBP Antibody [NBP2-29656] - rat cortex homogenate showing specific immunolabeling of the ~ 18 & 22k MBP protein.")
![Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP2-29656] - Immunofluorescence of a section of rat cerebellum showing specific labeling of myelin basic protein (1:5000, green) in the oligodendrocytes and myelin sheathes around axons. The section is colabeled with Anti-NFL (red) labeling dendrites and axons of neuronal cells. The blue stains nuclear DNA.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29656-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP2-29656] - Rat mixed neuron/glial cultures stained with chicken anti-Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29656-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP2-29656] - Immunostaining of cultured neonatal rat neurons and glia stained with anti-myelin basic protein antibody (red, 1:500). The blue stain is to identify nuclear DNA. Note the antibody stains the myelin basic protein located in oligodendrocyte and myelin sheathes around axons in the cells.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29656-img0004.jpg)
![Western Blot: SLC12A3 Antibody [NBP1-44270] - Western blot analysis of Rat tissue lysates showing detection of SLC12A3 protein using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:1000.](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Western-Blot-NBP1-44270-img0009.jpg "Western Blot: SLC12A3 Antibody [NBP1-44270] - Western blot analysis of Rat tissue lysates showing detection of SLC12A3 protein using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:1000.")
![Immunohistochemistry: SLC12A3 Antibody [NBP1-44270] - Immunohistochemistry analysis using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Immunohistochemistry-NBP1-44270-img0010.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody [NBP2-16380] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP2-16380-img0002.jpg "Western Blot: ERR alpha/NR3B1 Antibody [NBP2-16380] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:2000.")
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP2-16380] - Human intestine. ERR alpha stained by ERR alpha antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP2-16380-img0006.jpg)
![Chromatin Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Chromatin-Immunoprecipitation-NBP2-16380-img0003.jpg)
![Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380] - IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 ug of ERR alpha antibody 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary eagent.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunoprecipitation-NBP2-16380-img0005.jpg)
![Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Western-Blot-NBP2-50050-img0004.jpg "Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.")
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Adult mouse cortex (Left) and cerebellum (Right) sections were stained with NBP2-50050 in red, and co-stained with rabbit polyclonal anti-RBFOX3/NeuN antibody (NBP1-92716, green). Calbindin is expressed in a subset of interneurons in the cortex (Left) and prominently expressed in the dendrites of Purkinje cells in the cerebellum molecular layer (Right). RBFOX3/NeuN expresses in most neurons; as a result, cells positive for Calbindin appear to be yellow. Insets are high magnification images of the boxed area. Blue is Dapi nucleus staining that labels DNA.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Immunocytochemistry-Immunofluorescence-NBP2-50050-img0003.jpg)
![Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of rat brain cerebellum section stained with mouse mAb to Calbindin D-28K, dilution 1:2,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The Calbindin D-28K antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granular and white matter layers of cerebellum.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Immunohistochemistry-Free-Floating-NBP2-50050-img0006.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ug per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight (TM) 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block for30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.](http://images.novusbio.com/fullsize/alpha 2-Macroglobulin Antibody-Western Blot-NB600-938-img0010.jpg "Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ug per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight (TM) 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block for30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.")
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Analysis using the Biotin conjugate of NB600-938. Detection of Lane 1: . Lane 2: none. Load: 100 ug per lane. Primary antibody:alpha 2-Macroglobulin biotin conjugated antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0006.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Detection of Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0007.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 163 kDa, 163 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0009.jpg)
