| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89203-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78278SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27395 | |||
| BSA Free | NBP2-27396SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-676SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70207 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74572SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60491SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-81586SS | |||
![Immunohistochemistry: Vimentin Antibody (RV203) [NBP1-97671] - BTIC markers in our CNS-PNET model tumors.G: Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection) H: Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Immunohistochemistry-NBP1-97671-img0008.jpg "Immunohistochemistry: Vimentin Antibody (RV203) [NBP1-97671] - BTIC markers in our CNS-PNET model tumors.G: Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection) H: Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: Vimentin Antibody (RV203) [NBP1-97671] - Frozen section of human colon showing positive staining in connective tissue cells and no reactivity in epithelial cells](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Immunohistochemistry-Frozen-NBP1-97671-img0001.jpg)
![Flow Cytometry: Vimentin Antibody (RV203) [NBP1-97671] - An intracellular stain was performed on HeLa cells with NBP1-97671PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Flow-Cytometry-NBP1-97671-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) [NBP1-97671] - Paraffin section of human colon](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Immunohistochemistry-Paraffin-NBP1-97671-img0002.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV203) [NBP1-97671] - Figure 3. Immunohistochemistry with NBP1-97671 (RV203) on a frozen section of human kidney showing positive staining in blood vessels, glomeruli and connective tissue cells and no reactivity in epithelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Immunohistochemistry-Frozen-NBP1-97671-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) - BSA Free [NBP1-97671] - BTIC markers in our CNS-PNET model tumors.A: Ki67, 20X, insert- 5X digital (LCAS-R-12 weeks post-injection) B: Oct3/4, 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) C: Nestin 20x, insert- 5X digital (LCAS-R-12 weeks post-injection) D: Nestin 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) E: Sox2 40X, insert- 5X digital, (LC26-R-12 weeks post-injection) F: Sox2 40X, insert- 5X digital (LCAS-R-12 weeks post-injection) G: Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection) H: Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97671_mouse-monoclonal-vimentin-antibody-rv203-31020241534370.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) - BSA Free [NBP1-97671] - BTIC markers in our CNS-PNET model tumors.A: Ki67, 20X, insert- 5X digital (LCAS-R-12 weeks post-injection) B: Oct3/4, 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) C: Nestin 20x, insert- 5X digital (LCAS-R-12 weeks post-injection) D: Nestin 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) E: Sox2 40X, insert- 5X digital, (LC26-R-12 weeks post-injection) F: Sox2 40X, insert- 5X digital (LCAS-R-12 weeks post-injection) G: Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection) H: Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97671_mouse-monoclonal-vimentin-antibody-rv203-31020241535653.jpg)
![Immunohistochemistry: Vimentin Antibody (RV203) - BSA Free [NBP1-97671] - Analysis of RG, TCL & the TCL derived tumors(A1, A2) LCAS-R 2nd nsphrs/LCAS-RTL(138) 2nd nsphr, (A3) Tubb3 40x LCAS-RTL(138) 2nd nsphr, (A4) GFAP 40x LCAS-RTL(138) 2nd nsphr; (B1, B2) HE 40x LCAS-R/LCAS-RTL(138) - images show undifferentiated small round blue cells with scarce cytoplasm, (B3) HE 40x (LCAS-RTL(138) SVZ 15 weeks post-injection) - extensive proliferation of undifferentiated embryonal tumor cells infiltrating the adjacent brain parenchyma, (B4) Ki67 40x (LCAS-RTL(138) SVZ 15 weeks post-injection) - high proliferative activity of tumor cells is reflected by over 75% of cells expressing Ki-67, (C1) OCT3/4 40x (LC26-RTL(170) SVZ 12 weeks post-injection) - extensive proliferation of tumor cells invading adjacent brain parenchyma show high expression of OCT3/4 (over 80% of cells), (C2) Sox2 40x (LC26-RTL(170) SVZ 12 weeks post-injection) - extensively expressed in the tumor cells, (C3) PRAME 40x (LCAS-R 12 weeks post-injection) - extensively expressed in the tumor cells, (C4) Nestin 40x (LCAS-RTL(138) 15 weeks post-injection) - extensively expressed in the tumor cells, (D1) Vimentin 40x (LCAS-RTL(138) 15 weeks post-injection) - extensively expressed in the tumor cells within the main tumor mass & in the invading areas, (D2) BLBP 40x (LC26-RTL(170) 16 weeks post-injection) - extensively expressed in the tumor cells, (D3, D4) OTX2 20x, 40x (LC26-RTL(170) 16 weeks post-injection) - extensive expression (over 80% of cells) in tumor cells. TU-tumor; P-parenchyma; PI- perivascular invasion. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.24460), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97671_mouse-monoclonal-vimentin-antibody-rv203-310202416114726.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Western-Blot-NBP2-67483-img0013.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of LOVO cells labeling Lactate Dehydrogenase with Rabbit anti-Lactate Dehydrogenase A/LDHA antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lactate Dehydrogenase antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunocytochemistry-Immunofluorescence-NBP2-67483-img0014.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Lactate Dehydrogenase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0015.jpg)
![Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of Hela cells with Lactate Dehydrogenase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Flow-Cytometry-NBP2-67483-img0001.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysate using anti-Lactate Dehydrogenase antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Western-Blot-NBP2-67483-img0011.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Western blot analysis of Lactate Dehydrogenase A/LDHA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Western-Blot-NBP2-67483-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Staining Lactate Dehydrogenase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunocytochemistry-Immunofluorescence-NBP2-67483-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Staining Lactate Dehydrogenase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunocytochemistry-Immunofluorescence-NBP2-67483-img0003.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0004.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human liver cancer tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0005.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded human liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0006.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded mouse liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0007.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0008.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (SU39-06) [NBP2-67483] - Analysis of paraffin-embedded mouse testis tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-SU39-06-Immunohistochemistry-Paraffin-NBP2-67483-img0009.jpg)
![Western Blot: GFAP Antibody (012) [NBP2-89203] - Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight 800) at 1:10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 45 kDa.](http://images.novusbio.com/fullsize/GFAP-Antibody-012-Western-Blot-NBP2-89203-img0001.jpg "Western Blot: GFAP Antibody (012) [NBP2-89203] - Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight 800) at 1:10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 45 kDa.")
![Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20535-img0006.jpg "Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535] - Human breast carcinoma. SOD2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP2-20535-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-20535_rabbit-polyclonal-sod2-mn-sod-antibody-1610202419405789.jpg)
![Western Blot: Cytokeratin 19 Antibody [NBP1-78278] - Analysis of Cytokeratin 19 in MCF7 lysate.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Western-Blot-NBP1-78278-img0006.jpg "Western Blot: Cytokeratin 19 Antibody [NBP1-78278] - Analysis of Cytokeratin 19 in MCF7 lysate.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NBP1-78278] - Antibody at 1:25 in MCF7 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78278-img0004.jpg)
![Immunohistochemistry: Cytokeratin 19 Antibody [NBP1-78278] - Analysis of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunohistochemistry-NBP1-78278-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NBP1-78278] - Confocal immunofluorescent analysis of HepG2 cells using Cytokeratin 19 antibody (NBP1-78278, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78278-img0007.jpg)
![Simple Western: Cytokeratin 19 Antibody [NBP1-78278] - Image shows a specific band for Cytokeratin 19 in 0.05 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Simple-Western-NBP1-78278-img0008.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Hepatocyte protein lysate at 1:1000 4C overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Western-Blot-NBP2-27396-img0034.jpg "Western Blot: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Hepatocyte protein lysate at 1:1000 4C overnight. Image from verified customer review.")
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed, paraffin-embedded human liver tissue stained with HMGB1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0035.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Intracellular analysis using HMGB1 antibody. Human Jurkat cells were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Flow-Cytometry-NBP2-27396-img0021.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Analysis using 2 ug/ml concentration of HMGB1 antibody (clone 19N10B7) on (A) Full-length human HMGB1 protein, (B) Human Jurkat cell lysate and (C) Mouse NIH 3T3 cell lysate. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution was used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Western-Blot-NBP2-27396-img0033.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed, paraffin-embedded human spleen tissue stained with HMGB1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0022.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed, paraffin-embedded human breast stained with HMGB1 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0023.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed paraffin-embedded human prostate tissue stained with HMGB1 antibody at 10 ug/ml concentration. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0024.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Analysis of formalin-fixed, decalcified, paraffin embedded tissue section from the paws of mouse (collagen-induced arthritis model) using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. [Image courtesy of Dr Ulf Andersson, Karolinska Institute].](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0025.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of human stomach using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Distinct nuclear staining of HMGB1 was observed in different cells of the glandular stomach.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0026.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of normal human skin using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. The various cells of the epidermal layer showed intense nuclear staining along with weak cytoplasmic staining. The blood vessels, glandular cells and the other cells in dermal layer also showed nuclear positivity for HMGB1 immunostaining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0027.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of normal human brain using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Representative image shows a distinct nuclear immunostaining of HMGB1 in the various brain cells.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0028.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of normal human colon using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Representative image shows specific HMGB1 nuclear positivity in different mucosal cells of the colon.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0029.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of malignant stromal tumor of small bowel from human using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. HMGB1 immunopositivity of differential intensity was observed in the cells of tested section.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0030.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of human bladder cancer using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Strong nuclear HMGB1 immunopositivity was observed in the bladder cancer cells whereas the staining was weak in cells of tumor stroma.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0031.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of human stomach cancer using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. This representative image shows a distinct nuclear HMGB1 immunopositivity in the cancerous and sub-mucosal cells.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0032.jpg)
![Simple Western: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Simple Western lane view shows a specific band for HMGB1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Simple-Western-NBP2-27396-img0020.jpg)
![Knockout Validated: UCH-L1/PGP9.5 Antibody [NB300-676] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and UCH-L1/PGP9.5 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human UCH-L1/PGP9.5 Polyclonal Antibody (Catalog # NB300-676) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UCH-L1/PGP9.5 at approximately 28 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Knockout-Validated-NB300-676-img0013.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: UCH-L1/PGP9.5 Antibody [NB300-676] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and UCH-L1/PGP9.5 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human UCH-L1/PGP9.5 Polyclonal Antibody (Catalog # NB300-676) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UCH-L1/PGP9.5 at approximately 28 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: UCH-L1/PGP9.5 Antibody [NB300-676] - Analysis of UCHL1 in mouse brain lysate ECL detection, 60 seconds.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB300-676-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-676] - Neuro2a cells stained with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-676-img0007.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NB300-676] - Staining of neurons and cell processes at 2.5ug/mL. Human brain, Substantia Nigra, Pigmented Neurons, 4X.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunohistochemistry-Paraffin-NB300-676-img0009.jpg)
![Flow Cytometry: UCH-L1/PGP9.5 Antibody [NB300-676] - An intracellular stain was performed on U-87 cells with UCH-L1/PGP9.5 Antibody NB300-676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Flow-Cytometry-NB300-676-img0012.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody [NB300-676] - Image shows a specific band for PGP9.5/UCHL-1 in 0.05 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Simple-Western-NB300-676-img0011.jpg)
![Western Blot: BMAL1 Antibody (OTI1C11) [NBP2-02544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMAL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMAL1.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Western-Blot-NBP2-02544-img0013.jpg "Western Blot: BMAL1 Antibody (OTI1C11) [NBP2-02544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMAL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMAL1.")
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNTL .](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunocytochemistry-Immunofluorescence-NBP2-02544-img0014.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0012.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue N93ing anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0003.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded colon tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0006.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded lung tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0007.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded pancreas tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0008.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded prostate tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0009.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0010.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded bladder tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0011.jpg)
![Western Blot: SG2NA Antibody (S68) [NB110-74572] - Detection of SG2NA in NIH3T3 cell lysates using NB110-74572.](http://images.novusbio.com/fullsize/SG2NA-Antibody-S68-Western-Blot-NB110-74572-img0005.jpg "Western Blot: SG2NA Antibody (S68) [NB110-74572] - Detection of SG2NA in NIH3T3 cell lysates using NB110-74572.")
![Immunocytochemistry/Immunofluorescence: SG2NA Antibody (S68) [NB110-74572] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SG2NA (S68) [NB110-74572] at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SG2NA-Antibody-S68-Immunocytochemistry-Immunofluorescence-NB110-74572-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SG2NA Antibody (S68) [NB110-74572] - Immunofluorescent staining of NIH3T3 cells using NB110-74572.](http://images.novusbio.com/fullsize/SG2NA-Antibody-S68-Immunocytochemistry-Immunofluorescence-NB110-74572-img0002.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0023.jpg "Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract")
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - ARPE-19 monolayer on glass substrate. Fixative Methanol Blocking Goat Serum. Dilution 1:100. Incubation 1h @ RT. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-NB300-750-img0024.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0017.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of various human, mouse and non-human primate whole cell lysates.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0016.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0019.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of Pax6 in skate (Leucoraja erinacea) brain lysate using anti-Pax6 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NB300-750-img0011.jpg)
![Immunoprecipitation: Pax6 Antibody [NB300-750] - Analysis of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500ug whole cell lysate with 5ug of PAX6 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:1000 for at least one hour.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunoprecipitation-NB300-750-img0020.jpg)
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs).(a) Schematic description of the two-stage protocol applied to iPSC-renal commitment. (b, c, d, e) Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. (b) The pluripotency markers SSEA4, TRA-1-81, Nanog, ME marker T(Bry) & IM marker Osr1. (c) IM & MM marker expression as Wt1, Pax8, Pax2, Six2 & Sall1. (d) Renal progenitor markers CD133, CD24, NCAM, glomerular epithelial marker Claudin1 & proximal tubular epithelial markers AQP1, GGT1. (e) Markers identifying endodermal AFP, ectodermal Pax6, & cardiac mesodermal Nkx2.5 derivation. Nuclei are stained with DAPI (blue). Scale bars: 20 μm (b, c, d, e). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-750_rabbit-polyclonal-pax6-antibody-3102024161898.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Dynamin Monoclonal Antibody (Catalog # NB110-60491) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Dynamin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Knockout-Validated-NB110-60491-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Dynamin Monoclonal Antibody (Catalog # NB110-60491) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Dynamin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Simple Western: Dynamin Antibody (3G4B6) [NB110-60491] - Simple Western lane view shows a specific band for Dynamin in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Simple-Western-NB110-60491-img0012.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using Dynamin mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Western-Blot-NB110-60491-img0003.jpg)
![Immunoprecipitation: Dynamin Antibody (3G4B6) [NB110-60491] - Antibody-bead conjugates were prepared by adding 1.0 ug of antibody to 500 ul of PBS with 0,01% triton X-100 in a microcentrifuge tube and 30ul of protein G Sepharose beads. Tubes were rocked O/N at 4 degrees C followed by several washes to remove unbound antibodies. U2OS were collected in HEPES buffer (20 mM HEPES, 100 mM sodium chloride, 1 mM EDTA, 1% Triton X-100, pH 7.4) supplemented with protease inhibitor. Lysates are rocked 30 min at 4 degrees C and spun at 110,000xg for 15 min at 4 degrees C. One ml aliquots at 1 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4 degrees C. Following centrifugation, the unbound fractions were collected, and beads were subsequently washed three times with 1 ml of HEPES lysis buffer and processed for SDS-PAGE and immunoblot. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Immunoprecipitation-NB110-60491-img0014.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using anti-Dynamin monoclonal antibody against truncated Dynamin recombinant protein (1), SK-N-SH (2) and NIH3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Western-Blot-NB110-60491-img0002.jpg)
![Immunohistochemistry-Paraffin: Dynamin Antibody (3G4B6) [NB110-60491] - Immunohistochemical analysis of paraffin-embedded human Lymph tissue, glioma tissue and cerebellum tissue, showing membrane localization using Dynamin antibody with DAB staining.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Immunohistochemistry-Paraffin-NB110-60491-img0004.jpg)
![Flow Cytometry: Dynamin Antibody (3G4B6) [NB110-60491] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dynamin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Flow-Cytometry-NB110-60491-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Dynamin Antibody (3G4B6) [NB110-60491] - U2OS WT and DNM1 KO cells were labelled with a green or a far red fluorescence dye, respectively. WT and KO cells were mixed and plated to a 1 :1 ratio on coverslips. Cells were stained with and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (WT), red (antibody staining) and far-red (KO) channels was performed. Representative grayscale image of the red channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Antibody dilution used: 1/1000. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Knockout-Validated-NB110-60491-img0015.jpg)
![Western Blot: BAI1 Antibody [NB110-81586] - analysis of BAI1 in mouse brain tissue using anti-BAI1 antibody. KO mice was used as negative controls (right lane). Image from verified customer review.](http://images.novusbio.com/fullsize/BAI1-Antibody-Western-Blot-NB110-81586-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BAI1 Antibody [NB110-81586] - analysis of BAI1 in mouse brain tissue using anti-BAI1 antibody. KO mice was used as negative controls (right lane). Image from verified customer review.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BAI1 Antibody [NB110-81586] - BAI1 antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/BAI1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-81586-img0001.jpg)
![Immunohistochemistry-Paraffin: BAI1 Antibody [NB110-81586] - A. Staining of mouse brain using NB110-81586. B. Blocking of staining using control peptide, NB110-81586PEP.](http://images.novusbio.com/fullsize/BAI1-Antibody-Immunohistochemistry-Paraffin-NB110-81586-img0003.jpg)