| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04676SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23610SS | |||
| Azide and BSA Free | NBP2-80919 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73526 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35370-20ul | |||
![HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with PGC1 alpha Antibody [NBP1-04676] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PGC1-alpha-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-04676-img0010.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Effect of butein T on SC adipose tissue browning in the ThermoMouse. SC fat was obtained from ThermoMouse exposed for 4 days to butein (10 or 20 mg/kg; n = 4) or vehicle (n = 2). A BAT lysate of a ThermoMouse was used as a positive control for UCP-1 protein levels. Tissues were homogenized & protein lysates (50 µg for SC fat & 10 µg for BAT) were used in western blotting for PGC1-alpha (a) & UCP-1 (c). Panels (b) & (d) illustrate the densitometric quantitation of the PGC1-alpha & UCP-1 western blot, respectively, normalized for the levels of the loading control alpha -tubulin. Abbreviations: subcutaneous (SC); brown adipose tissue (BAT); peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC1-alpha ) & uncoupling protein-1 (UCP-1). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31094273), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-310202415291971.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Expression of the mitochondrial biogenesis proteins: SIRT1, PGC1 alpha , TFAM & SIRT3 in H1299, H1299r, P31 & P31r cell lysates as determined by immunoblot analysisWhole cell lysates were prepared from confluent cultures of H1299, H1299r, P31 & P31r cells. Proteins (90 μg) were resolved in 10% SDS-PAGE gels & transferred to a PVDF membrane. Blots were probed for SIRT1, PGC1 alpha , TFAM & SIRT3 or the loading control gamma -tubulin. Figure shows representative blots of (A) of SIRT1, (B) PGC1 alpha , (C) SIRT3, (D) TFAM protein expression; each from three independent experiments. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.21885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-31020241534320.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Maternal exercise during pregnancy on mitochondrial biogenesis in the fetal hearts. (A) Levels of relative mRNA expression measured by qRT‐PCR. n = 9–12/group. Maternal exercise during pregnancy did not alter levels of mRNA in Ppargc1a & Tfam, while it significantly upregulated the levels of mRNA in Nrf1 & Nrf2. (B–D) Densitometric analyses of protein expression levels relative to the sedentary group with representative images of western blots were shown. No significant differences in PGC‐1 alpha , NRF1, & NRF2 (P > 0.05). n = 5–6/group. * P < 0.05, significantly different from the sedentary group. Black bar: fetal hearts from sedentary dams; gray bar: fetal hearts from exercised dams. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28292876), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-310202415345322.jpg)
![Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody - BSA Free [NBP1-04676] - DM1-derived fibroblasts have no changes in mitochondria biogenesis. (A, B) Representative images of immunofluorescence of TOMM20, & PGC1-alpha in DM1 & control fibroblasts (n=3). (C) Medium fluorescence intensity of MitoTracker Red FM in control (n=3) & DM1 cells (n=5) & (D) of Rhodamine 123 in DM1 & control fibroblasts (n=3). (E) mRNA levels of TFAM transcription factor (n=3). (F) mRNA levels of OPA1, MFN1, MFN2, DRP1 & PARKIN in DM1 & control fibroblasts (n≥2). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32310829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-3102024153566.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Effect of BGP-15 treatment on the regulation of mitochondrial biogenesis in NRCM cells. Western blot analysis of PGC-1-alpha , CREB, & VDAC proteins as well as densitometric evaluation is shown. GAPDH was used as a loading control. Control group: cells without any treatment; BGP-15 group: cells with only 50 μM BGP-15 for 0.5 hours; H2O2 group: cells with 150 μM H2O2 for 0.5 hours; H2O2+BGP-15 group: cells with 150 μM H2O2 & 50 μM BGP-15 for 0.5 hours. Values are mean ± SEM (n = 4). ∗p < 0.05 vs. Control, ∗∗p < 0.01 vs. Control, §§p < 0.01 vs H2O2 group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33728024), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-310202415334945.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Feeding the diabetes mellitus (DM) mice with the RSV‐enriched diet improved mitochondrial biogenesis in the quadriceps muscle. A) Representative western blots using antibodies against nuclear respiratory factor (NRF‐1), ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), cytochrome c oxidase subunit IV (Cox IV), peroxisome proliferator‐activated receptor‐ gamma coactivator 1‐ alpha (PGC‐1 alpha ), & GAPDH. After quantification, the B) NRF‐1/GAPDH, C) ATP5B/GAPDH, D) Cox IV/GAPDH, & E) PGC‐1 alpha /GAPDH ratios were calculated in mice fed either the control (CTL) or RSV‐enriched diet. GAPDH was used as a loading control. F) Representative western blots using antibodies against mitochondrial transcription factor A (mtTFA) & voltage‐dependent anion channel (VDAC), & the mtTFA/VDAC ratio was determined. The mitochondrial protein VDAC was used as a loading control. Data are expressed as fold change versus CTL & reported as mean ± SD, n = 4–6 per group; *p < 0.05; **p < 0.01 between CTL & DM groups. #p < 0.05 between DM & DM+RSV groups; ††p < 0.05 between CTL+RSV & DM+RSV groups. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29578301), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-310202416171473.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Effects of SRTAW04 on expression of markers of mitochondrial & anti-oxidant function. (a) Western blot of protein extracts from optic nerve & retina of control (lanes 1–4), MHV-A59 infected (lanes 5–8), & MHV-A59 infected + SRTAW04-treated (lanes 9–12) mice. Average levels of SDHb measured by Western blotting (n = 4/group) showed a significant (*p < 0.05) decrease in protein extracts from optic nerves (a,b) & retinas (a,d) of MHV-A59 infected mice 7 days post-inoculation, compared to control mice. MHV-A59 infected mice treated with SRTAW04 (100 mg/kg/day) showed a significant increase (@p < 0.05) of SDHb protein expression compared to untreated MHV-A59 infected mice. There is a significant decrease (*p < 0.05) in expression of SOD2 (n = 4/group) in optic nerves (a,c) & retinas (a,e) of MHV-A59 infected mice compared to control mice, & treatment with SRTAW04 significantly (@p < 0.05) attenuates that change. PGC1-alpha expression shows a significant (*p < 0.05) decrease in retinas (a,f) (n = 4/group) during MHV-A59 infection & treatment with SRTAW04 for 7 days significantly (@p < 0.05) increases the PGC1-alpha protein levels. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24383546), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-31020241616036.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - RSV treatment increases markers of mitochondrial function in stressed RGC-5 cells. Staurosporine-differentiated RGC-5 cells were cultured with or without 500 μM H2O2, & with or without 0.25 μM RSV for 24 h. (A) Western blot analysis shows a significant decrease in SDH expression (**p < 0.01) during H2O2 treatment which is attenuated by treatment with RSV (*p < 0.05). (B) Western blot analysis shows similar effects on SOD2 expression. The significant decrease (**p < 0.01) during H2O2 treatment, compared to controls, is not found in cells treated with RSV (*p < 0.05). (C) Protein extracts were immunoprecipitated with anti-PGC-1 alpha antibodies, blotted, & hybridized with anti-PGC-1 alpha & anti-acetylated lysine antibodies to assess the acetylation state of PGC-1 alpha . H2O2 treatment significantly increases the proportion of acetylated PGC-1 alpha (*p < 0.05) compared to controls, & RSV & SRTAW04 treatment each prevent this acetylation (*p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23293585), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-310202416154646.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Pequi extract increases expression of antioxidant enzymes in HCAEC. Cells were cultured, pre-treated with pequi extract (25 μg/mL) for 24 h under normoxia conditions, & total protein content was extracted for Western blots analysis for Superoxide dismutase (SOD)-1 (A), SOD-2 (B), catalase (C), glutathione peroxidase—GPx (D), sirtuin 1—SIRT-1 (E), peroxisome-proliferator-activated receptor gamma coactivator 1-alpha (PCG1-alpha ) (F), glycogen synthase kinase 3 beta (GSK3 beta ) (G), & microtubule-associated protein 1A/1B-light chain 3 (LC3) (H). Western blots were normalized using alpha -tubulin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as loading control. Five independent Western blot experiments were carried for each condition. Representative images are shown here. Statistical analysis was performed using Student’s t-test (p < 0.05). OD: Optical Density. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35326129), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-31020241618267.jpg)
![Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Role of PGC1 alpha in NIX-dependent mitophagy.a–e Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 12 h, respectively. a, b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha ) expression was detected in western blot where beta -actin was used as a loading control in both cell types. n = 5. c Colocalization of PGC1 alpha (red) & DAPI (blue) in hippocampal neurons was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. d Colocalization of PGC1 alpha (green) & DAPI (blue) in SH-SY5Y was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. e PGC1 alpha protein expressions in subcellular fraction samples were detected by western blotting. Lamin A/C & alpha -tubulin were used as a nuclear & cytosolic loading control, respectively. n = 5. f, g SH-SY5Y cells were transfected with pcDNA3.1/c-eGFP or pcDNA3.1/PPARGC1A-c-eGFP vector for 24 h prior to cortisol treatment for 24 h. f NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. g TOMM20 levels were detected by western blot. Loading control for western blot is beta -actin. n = 5. All blots & immunofluorescence images are representative. n = 5 from independent experiments with two technical replicates each. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted. ** indicates p < 0.01 versus control. #, ## indicates p < 0.05, p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-310202416154631.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Role of PGC1 alpha in NIX-dependent mitophagy.a–e Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 12 h, respectively. a, b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha ) expression was detected in western blot where beta -actin was used as a loading control in both cell types. n = 5. c Colocalization of PGC1 alpha (red) & DAPI (blue) in hippocampal neurons was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. d Colocalization of PGC1 alpha (green) & DAPI (blue) in SH-SY5Y was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. e PGC1 alpha protein expressions in subcellular fraction samples were detected by western blotting. Lamin A/C & alpha -tubulin were used as a nuclear & cytosolic loading control, respectively. n = 5. f, g SH-SY5Y cells were transfected with pcDNA3.1/c-eGFP or pcDNA3.1/PPARGC1A-c-eGFP vector for 24 h prior to cortisol treatment for 24 h. f NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. g TOMM20 levels were detected by western blot. Loading control for western blot is beta -actin. n = 5. All blots & immunofluorescence images are representative. n = 5 from independent experiments with two technical replicates each. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted. ** indicates p < 0.01 versus control. #, ## indicates p < 0.05, p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-31020241616560.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - EMPA treatment increased mitochondrial biogenesis. (A) Left, Transmission electron microscopy showing the morphology of mitochondria in sham, sham + EMPA, TAC & TAC + EMPA groups & Right, quantitative analysis of mitochondrial size & counts. Results are expressed as mean ± SEM, n = 3–5, *p < 0.05 vs. corresponding sham, †p < 0.05 vs. corresponding TAC. (B) Left, Representative blots of mitochondrial biogenesis-related proteins & Right, quantitative results. (C) Relative mRNA levels of PGC1 alpha , NRF1, TFAM & COX1. Results are expressed as mean ± SEM, n = 5–7, *p < 0.05 vs. corresponding sham group, †p < 0.05 vs. corresponding TAC vehicle group. One-way ANOVA & Tukey post hoc test. EMPA, empagliflozin; SEM, standard error of the mean; TAC, transverse aortic constriction; PGC1-alpha , peroxisome proliferator-activated receptor gamma coactivator 1-alpha; NRF-1, nuclear respiratory factor 1; TFAM, mitochondrial transcription factor A; COX1, cyclooxygenase1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35647080), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-310202416182622.jpg)
![Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Role of PGC1 alpha in NIX-dependent mitophagy.a–e Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 12 h, respectively. a, b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha ) expression was detected in western blot where beta -actin was used as a loading control in both cell types. n = 5. c Colocalization of PGC1 alpha (red) & DAPI (blue) in hippocampal neurons was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. d Colocalization of PGC1 alpha (green) & DAPI (blue) in SH-SY5Y was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. e PGC1 alpha protein expressions in subcellular fraction samples were detected by western blotting. Lamin A/C & alpha -tubulin were used as a nuclear & cytosolic loading control, respectively. n = 5. f, g SH-SY5Y cells were transfected with pcDNA3.1/c-eGFP or pcDNA3.1/PPARGC1A-c-eGFP vector for 24 h prior to cortisol treatment for 24 h. f NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. g TOMM20 levels were detected by western blot. Loading control for western blot is beta -actin. n = 5. All blots & immunofluorescence images are representative. n = 5 from independent experiments with two technical replicates each. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted. ** indicates p < 0.01 versus control. #, ## indicates p < 0.05, p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-310202416173517.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Role of PGC1 alpha in NIX-dependent mitophagy.a–e Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 12 h, respectively. a, b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha ) expression was detected in western blot where beta -actin was used as a loading control in both cell types. n = 5. c Colocalization of PGC1 alpha (red) & DAPI (blue) in hippocampal neurons was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. d Colocalization of PGC1 alpha (green) & DAPI (blue) in SH-SY5Y was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. e PGC1 alpha protein expressions in subcellular fraction samples were detected by western blotting. Lamin A/C & alpha -tubulin were used as a nuclear & cytosolic loading control, respectively. n = 5. f, g SH-SY5Y cells were transfected with pcDNA3.1/c-eGFP or pcDNA3.1/PPARGC1A-c-eGFP vector for 24 h prior to cortisol treatment for 24 h. f NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. g TOMM20 levels were detected by western blot. Loading control for western blot is beta -actin. n = 5. All blots & immunofluorescence images are representative. n = 5 from independent experiments with two technical replicates each. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted. ** indicates p < 0.01 versus control. #, ## indicates p < 0.05, p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-31020241615460.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Mitochondrial content & PGC‐1 alpha levels are increased in cells with greater TFEB protein expression. (a) Mitochondrial content was measured in SH‐SY5Y cells & TFEB‐DDK cells under basal conditions by assaying citrate synthase (CS) activity. **p < 0.01 versus SH‐SY5Y; n = 6. (b) Western blotting for the mitochondrial proteins prohibitin 1 & cytochrome oxidase (COX) subunit IV also indicated that mitochondrial content was increased in TFEB‐DDK cells. (c) PGC‐1 alpha mRNA levels were measured in SH‐SY5Y cells & TFEB‐DDK cells under basal conditions by qPCR. Data normalised against beta ‐actin mRNA levels. **p < 0.01 versus SH‐SY5Y cells; n = 3. (d) SH‐SY5Y cells were treated with scrambled (scram) or TFEB siRNA for 72 h & PGC‐1 alpha mRNA levels measured. *p < 0.05 versus scram; n = 4. (e) PGC‐1 alpha protein levels were detected by western blotting in total cell lysates of SH‐SY5Y cells, TFEB‐DDK cells & a SH‐SY5Y cell line over‐expressing human PGC‐1 alpha . The fold increase in PGC‐1 alpha protein density for this blot & PGC‐1 alpha mRNA levels of the respective cell lines are reported underneath the blot. (f) TFEB‐DDK cells were treated with 10 μM carbonyl cyanide m‐chlorophenylhydrazone (CCCP) for 18 h & cytosolic & nuclear fractions prepared. PGC‐1 alpha protein was detected in cytosolic (3.5% of total volume) & nuclear fractions (35% of total volume) by western blotting. The purity of the nuclear & cytosolic fractions was assessed by western blotting using lamin A & beta ‐actin antibodies respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26509433), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-31020241617358.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - PGC1 alpha & HNF4 alpha interacts & binds to Vnn1 promoter in IUGR asthmatic mice. Asthma was induced with OVA in IUGR mice. PBS induction was used as the control. Nuclear protein was extracted from lung tissues. (A) Immunoblot assay was performed for expressions of PGC1 alpha & HNF4 alpha . (B) IP was performed using mouse anti-HNF4 alpha antibody & Protein G-coupled agarose beads, followed by immunoblot with rabbit-anti-PGC1 alpha antibody. The normal mouse IgG was used as the IP control. (C) In primary cultured bronchial epithelia cells isolated from IUGR mice injected with OVA or PBS, ChIP assay was performed using mouse anti-HNF4 alpha or rabbit anti-PGC1 alpha antibodies. The normal mouse or rabbit IgG was used as the control. Graphics show the percentage of total DNA immunoprecipitated by each indicated antibody. Data are shown as mean±s.d. n=4. *P<0.01 OVA versus PBS. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32139393), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-31020241616088.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Role of PGC1 alpha in NIX-dependent mitophagy.a–e Nontargeting (NT) or GR siRNA was transfected to hippocampal neurons & SH-SY5Y cells for 24 h prior to corticosterone & cortisol for 12 h, respectively. a, b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha ) expression was detected in western blot where beta -actin was used as a loading control in both cell types. n = 5. c Colocalization of PGC1 alpha (red) & DAPI (blue) in hippocampal neurons was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. d Colocalization of PGC1 alpha (green) & DAPI (blue) in SH-SY5Y was visualized with SRRF imaging system. Scale bars, 20 μm (magnification, ×1000). n = 5. e PGC1 alpha protein expressions in subcellular fraction samples were detected by western blotting. Lamin A/C & alpha -tubulin were used as a nuclear & cytosolic loading control, respectively. n = 5. f, g SH-SY5Y cells were transfected with pcDNA3.1/c-eGFP or pcDNA3.1/PPARGC1A-c-eGFP vector for 24 h prior to cortisol treatment for 24 h. f NIX expression was detected in western blot where beta -actin was used as a loading control. n = 5. g TOMM20 levels were detected by western blot. Loading control for western blot is beta -actin. n = 5. All blots & immunofluorescence images are representative. n = 5 from independent experiments with two technical replicates each. Quantitative data are presented as a mean ± S.E.M. Two-sided two-way ANOVA was conducted. ** indicates p < 0.01 versus control. #, ## indicates p < 0.05, p < 0.01 versus corticosterone in hippocampal neurons & cortisol in SH-SY5Y, respectively. Data are provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-310202416175288.jpg)
![Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Effects of SRTAW04 on expression of markers of mitochondrial & anti-oxidant function. (a) Western blot of protein extracts from optic nerve & retina of control (lanes 1–4), MHV-A59 infected (lanes 5–8), & MHV-A59 infected + SRTAW04-treated (lanes 9–12) mice. Average levels of SDHb measured by Western blotting (n = 4/group) showed a significant (*p < 0.05) decrease in protein extracts from optic nerves (a,b) & retinas (a,d) of MHV-A59 infected mice 7 days post-inoculation, compared to control mice. MHV-A59 infected mice treated with SRTAW04 (100 mg/kg/day) showed a significant increase (@p < 0.05) of SDHb protein expression compared to untreated MHV-A59 infected mice. There is a significant decrease (*p < 0.05) in expression of SOD2 (n = 4/group) in optic nerves (a,c) & retinas (a,e) of MHV-A59 infected mice compared to control mice, & treatment with SRTAW04 significantly (@p < 0.05) attenuates that change. PGC1-alpha expression shows a significant (*p < 0.05) decrease in retinas (a,f) (n = 4/group) during MHV-A59 infection & treatment with SRTAW04 for 7 days significantly (@p < 0.05) increases the PGC1-alpha protein levels. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24383546), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04676_rabbit-polyclonal-pgc1-alpha-antibody-310202416163780.jpg)
![Flow Cytometry: PGC1 alpha Antibody [NB100-60955] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-16520239183346.jpg "Flow Cytometry: PGC1 alpha Antibody [NB100-60955] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-60955] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-1652023918336.jpg)
![Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NB100-60955] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-16520239183311.jpg)
![Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NB100-60955] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-1652023918331.jpg)
![Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-60955] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-60955_goat-polyclonal-pgc1-alpha-antibody-imgenex-img-30341-16520239183335.jpg)
![Western Blot: CPT1A Antibody [NB100-53791] - Semiquantitative immunoblotting of CPT1A. Protein expression was investigated in the HB of WT EAE (n=6) and Cpt1a P479L EAE mice (n=6). The data were normalized to the density of the beta actin control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31527712/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NB100-53791-img0002.jpg "Western Blot: CPT1A Antibody [NB100-53791] - Semiquantitative immunoblotting of CPT1A. Protein expression was investigated in the HB of WT EAE (n=6) and Cpt1a P479L EAE mice (n=6). The data were normalized to the density of the beta actin control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31527712/) licensed under a CC-BY license.")
![Western Blot: CPT1A Antibody [NB100-53791] - Staining of MCF7 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NB100-53791-img0001.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-53791_goat-polyclonal-cpt1a-antibody-16520239183313.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-53791_goat-polyclonal-cpt1a-antibody-16520239183326.jpg)
![Western Blot: UCP3 Antibody [NBP2-24608] - Analysis of UCP3 in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart](http://images.novusbio.com/fullsize/UCP3-Antibody-Western-Blot-NBP2-24608-img0001.jpg "Western Blot: UCP3 Antibody [NBP2-24608] - Analysis of UCP3 in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart")
![Western Blot: PON1 Antibody (4G8D3) [NBP2-23610] - Western blot analysis of PON1 in (1) HEK293 and (2) PON1 hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/PON1-Antibody-4G8D3-Western-Blot-NBP2-23610-img0007.jpg "Western Blot: PON1 Antibody (4G8D3) [NBP2-23610] - Western blot analysis of PON1 in (1) HEK293 and (2) PON1 hIgGFc transfected HEK293 cell lysate.")
![Immunohistochemistry: PON1 Antibody (4G8D3) [NBP2-23610] - Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues with DAB staining.](http://images.novusbio.com/fullsize/PON1-Antibody-4G8D3-Immunohistochemistry-NBP2-23610-img0006.jpg)
![Flow Cytometry: PON1 Antibody (4G8D3) [NBP2-23610] - Flow cytometric analysis of Hela cells using PON1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PON1-Antibody-4G8D3-Flow-Cytometry-NBP2-23610-img0004.jpg)
![Western Blot: PON1 Antibody (4G8D3) [NBP2-23610] - Western blot analysis of PON1 in human PON1 recombinant protein. (Expected MW is 40.6 kDa).](http://images.novusbio.com/fullsize/PON1-Antibody-4G8D3-Western-Blot-NBP2-23610-img0002.jpg)
![Western Blot: PON1 Antibody (4G8D3) [NBP2-23610] - Western blot analysis of PON1 in human plasma cell lysate.](http://images.novusbio.com/fullsize/PON1-Antibody-4G8D3-Western-Blot-NBP2-23610-img0003.jpg)
![Immunohistochemistry: PON1 Antibody (4G8D3) [NBP2-23610] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues with DAB staining.](http://images.novusbio.com/fullsize/PON1-Antibody-4G8D3-Immunohistochemistry-NBP2-23610-img0005.jpg)
![Western Blot: PON1 Antibody (OTI2D4) [NBP2-03706] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Western-Blot-NBP2-03706-img0004.jpg "Western Blot: PON1 Antibody (OTI2D4) [NBP2-03706] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1.")
![Immunocytochemistry/Immunofluorescence: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PON1.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Immunocytochemistry-Immunofluorescence-NBP2-03706-img0008.jpg)
![Immunohistochemistry-Paraffin: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of paraffin-embedded Human prostate tissue using anti-PON1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Immunohistochemistry-Paraffin-NBP2-03706-img0007.jpg)
![Flow Cytometry: PON1 Antibody (OTI2D4) [NBP2-03706] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PON1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Flow-Cytometry-NBP2-03706-img0003.jpg)
![Immunohistochemistry-Paraffin: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PON1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Immunohistochemistry-Paraffin-NBP2-03706-img0005.jpg)
![Immunohistochemistry-Paraffin: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of paraffin-embedded Human endometrium tissue using anti-PON1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Immunohistochemistry-Paraffin-NBP2-03706-img0006.jpg)
![Flow Cytometry: PON1 Antibody (OTI2D4) [NBP2-03706] - Analysis of Hela cells, using anti-PON1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Flow-Cytometry-NBP2-03706-img0001.jpg)
![Flow Cytometry: PON1 Antibody (OTI2D4) [NBP2-03706] - Analysis of Jurkat cells, using anti-PON1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Flow-Cytometry-NBP2-03706-img0002.jpg)
![Western Blot: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Western-Blot-NBP2-73526-img0001.jpg "Western Blot: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1.")
![Immunocytochemistry/Immunofluorescence: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PON1.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73526-img0005.jpg)
![Immunohistochemistry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Staining of paraffin-embedded Human endometrium tissue using anti-PON1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73526-img0007.jpg)
![Flow Cytometry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PON1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73526-img0004.jpg)
![Immunohistochemistry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PON1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73526-img0006.jpg)
![Immunohistochemistry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Staining of paraffin-embedded Human prostate tissue using anti-PON1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73526-img0008.jpg)
![Flow Cytometry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Analysis of Hela cells, using anti-PON1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73526-img0002.jpg)
![Flow Cytometry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Analysis of Jurkat cells, using anti-PON1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73526-img0003.jpg)
![Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of Mouse liver, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PON1-Antibody-4X7X2-Western-Blot-NBP3-16187-img0005.jpg "Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of Mouse liver, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of HepG2 cells, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/PON1-Antibody-4X7X2-Western-Blot-NBP3-16187-img0006.jpg)
![Immunohistochemistry-Paraffin: PON1 Antibody (4X7X2) [NBP3-16187] - Immunohistochemistry of paraffin-embedded human liver using PON1 Rabbit mAb (NBP3-16187) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PON1-Antibody-4X7X2-Immunohistochemistry-Paraffin-NBP3-16187-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Rat liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16187_rabbit-pon1-mab-4x7x2-52202511392230.jpg)
![Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Mouse liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16187_rabbit-pon1-mab-4x7x2-5220251255594.jpg)
![Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Human liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16187_rabbit-pon1-mab-4x7x2-5220251242555.jpg)
![Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Wild-type (WT) and CPT1A knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249422686.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Wild-type (WT) and CPT1A knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded dog intestine. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249393596.jpg)
![Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-21202494084.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded cat tissues. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249383128.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249351354.jpg)
![Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25423_rabbit-cpt1a-mab-hl1164-azide-and-bsa-free-2120249393541.jpg)
![Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-1750] - Section of human colon adenocarcinoma. Antibody: Affinity purified rabbit PGC1 alpha Antibody used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/PGC1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-1750-img0006.jpg "Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-1750] - Section of human colon adenocarcinoma. Antibody: Affinity purified rabbit PGC1 alpha Antibody used at a dilution of 1:250. Detection: DAB")
![Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-1750] - Section of human stomach adenocarcinoma. Antibody: Affinity purified rabbit PGC1 alpha Antibody used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/PGC1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-1750-img0004.jpg)
![Immunohistochemistry: CPT1A Antibody [NBP3-35370] - Immunohistochemistry analysis of paraffin-embedded Human colon using CPT1A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35370_rabbit-cpt1a-pab-101220241153586.jpg "Immunohistochemistry: CPT1A Antibody [NBP3-35370] - Immunohistochemistry analysis of paraffin-embedded Human colon using CPT1A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: CPT1A Antibody [NBP3-35370] - Western blot analysis of lysates from HeLa cells using CPT1A Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35370_rabbit-cpt1a-pab-1012202411552896.jpg)
![Immunocytochemistry/ Immunofluorescence: CPT1A Antibody [NBP3-35370] - Immunofluorescence analysis of Hep G2 cells using CPT1A Rabbit pAbat a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35370_rabbit-cpt1a-pab-31120251704717.jpg)
![Western Blot: PON1 Antibody (JE30-90) [NBP3-32843] - Western blot analysis of PON1 on different lysates with Rabbit anti-PON1 antibody (NBP3-32843) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32843) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32843_rabbit-pon1-mab-je30-90-288202412461639.jpg "Western Blot: PON1 Antibody (JE30-90) [NBP3-32843] - Western blot analysis of PON1 on different lysates with Rabbit anti-PON1 antibody (NBP3-32843) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32843) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PON1 Antibody (JE30-90) [NBP3-32843] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-PON1 antibody (NBP3-32843) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32843) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32843_rabbit-pon1-mab-je30-90-298202414304136.jpg)
![Flow Cytometry: PON1 Antibody (JE30-90) [NBP3-32843] - Flow cytometric analysis of HepG2 cells labeling PON1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32843, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32843_rabbit-pon1-mab-je30-90-29202416453293.jpg)