| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-11427SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-147SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13249-0.2mg | |||
| BSA Free | NB120-13249-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50036SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43808 | |||
| BSA Free | NBP2-46617-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80993-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43809 | |||
| BSA Free | NBP2-46635-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50038SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43810 | |||
| BSA Free | NBP2-46636-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-43649-25ul | |||
| Azide and BSA Free | NBP3-44164 | |||
![Western Blot: Parvalbumin Antibody [NB120-11427] - WB analysis of rat cerebellum extract.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NB120-11427-img0002.jpg "Western Blot: Parvalbumin Antibody [NB120-11427] - WB analysis of rat cerebellum extract.")
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in U251 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0008.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin and parvalbumin antibodies shown in layer 2/3 at 63x magnification (scale = 10 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2013.00156/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in C6 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in Hela Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0007.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human cerebellum tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0003.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human skeletal muscle tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0004.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human tonsil tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427] - Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin & parvalbumin antibodies shown in layer 2/3 at 63× magnification (scale = 10 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24065886), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11427_rabbit-polyclonal-parvalbumin-antibody-310202415284515.jpg)
![Immunohistochemistry: Parvalbumin Antibody - BSA Free [NB120-11427] - Female Ambra1+/− mice show reduced numbers of hippocampal PV interneurons. a Nissl staining of dorsal hippocampus coronal sections from WT & Ambra1+/− female mice (scale bar: 500 μm). b Subdivision of hippocampal layers (stratum oriens, CA1 pyramidal layer, stratum radiatum, stratum lacunosum moleculare, stratum moleculare, dentate gyrus upper blade, hilus, dentate gyrus lower blade), corresponding to the area depicted in a (scale bar: 100 μm). Ambra1+/− females show normal hippocampal layering (n = 3 mice per genotype). c Double labeling for Ambra1 (green) & PV (red) in the CA1 from a WT female; sections were DAPI-counterstained (blue) for neuronal nuclei (scale bar: 50 μm). The higher magnification image (scale bar, 5 μm) from the stratum pyramidale shows a PV neuron (right; merge in orange) & an unidentified neuron (left), both expressing Ambra1. The DAPI signal is omitted for clarity & neuron borders are indicated by a dash line (n = 3 mice; three sections per animal). d, e Immunohistochemical labeling of PV in a coronal brain section of dorsal hippocampus obtained from female (d) & male (e) mice (scale bars: 500 μm). On the right are higher magnification images (scale bars: 50 μm). The plots show stereological quantification of PV interneurons (n = 6 mice per genotype & per gender; 14 sections per animal). The reduction in PV interneuron numbers is selective for female Ambra1+/− (two-tailed unpaired t test: *P = 0.043) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29488136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11427_rabbit-polyclonal-parvalbumin-antibody-310202416205149.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Western-Blot-NB300-147-img0005.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.")
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Sodium Potassium ATPase Beta 1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Immunocytochemistry-Immunofluorescence-NB300-147-img0008.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Detection of Na, K-ATPase (beta) in rat kidney homogenates (20 ug)using NB 300-147. Lane 1: 1:2,500 primary. Lane 2: 1:5,000 primary.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Western-Blot-NB300-147-img0007.jpg)
![Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat brain tissue lysate.](http://images.novusbio.com/fullsize/Bassoon-Antibody-SAP7F407-Western-Blot-NB120-13249-img0001.jpg "Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat brain tissue lysate.")
![Immunocytochemistry/Immunofluorescence: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat Hippocampal Neuron, image courtesy of customer.](http://images.novusbio.com/fullsize/Bassoon-Antibody-SAP7F407-Immunocytochemistry-Immunofluorescence-NB120-13249-img0003.jpg)
![Immunohistochemistry: Bassoon Antibody (SAP7F407) [NB120-13249] - Bassoon localization in the rat brain cerebellar molecular layer using Bassoon Monoclonal Antibody.](http://images.novusbio.com/fullsize/Bassoon-Antibody-SAP7F407-Immunohistochemistry-NB120-13249-img0002.jpg)
![Western Blot: Parvalbumin Antibody [NBP2-50036] - Analysis of skeletal muscle lysates and His-tagged recombinant human proteins using chicken parvalbumin pAb, dilution 1:2,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] recombinant full length human parvalbumin, [4] recombinant full length human calretinin and [5] recombinant full length human calbindin. Band at 12kDa in muscle lysate is native parvalbumin and 18kDa band in the next lane is His-tagged recombinant parvalbumin. The parvalbumin antibody binds parvalbumin strongly but is not cross-reactive with the related calcium binding proteins calretinin and calbindin.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NBP2-50036-img0005.jpg "Western Blot: Parvalbumin Antibody [NBP2-50036] - Analysis of skeletal muscle lysates and His-tagged recombinant human proteins using chicken parvalbumin pAb, dilution 1:2,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] recombinant full length human parvalbumin, [4] recombinant full length human calretinin and [5] recombinant full length human calbindin. Band at 12kDa in muscle lysate is native parvalbumin and 18kDa band in the next lane is His-tagged recombinant parvalbumin. The parvalbumin antibody binds parvalbumin strongly but is not cross-reactive with the related calcium binding proteins calretinin and calbindin.")
![Immunohistochemistry Free-Floating: Parvalbumin Antibody [NBP2-50036] - Analysis of a rat cerebellum section stained with chicken pAb to parvalbumin, NBP2-50036, dilution 1:2,000, in green, and costained with mouse mAb to GFAP, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. NBP2-50036 antibody labels the perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astrocytes.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunofluorescence-NBP2-50036-img0004.jpg)
![Western Blot: CNPase Antibody (CL2887) [NBP2-46617] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2887-Western-Blot-NBP2-46617-img0009.jpg "Western Blot: CNPase Antibody (CL2887) [NBP2-46617] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunocytochemistry/Immunofluorescence: CNPase Antibody (CL2887) [NBP2-46617] - Staining of RH-30 cells using the Anti-CNP monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2887-Immunocytochemistry-Immunofluorescence-NBP2-46617-img0010.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617] - Staining of human pancreas shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2887-Immunohistochemistry-NBP2-46617-img0007.jpg)
![Western Blot: CNPase Antibody (CL2887) [NBP2-46617] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2887-Western-Blot-NBP2-46617-img0008.jpg)
![Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617] - Staining of rat cerebral cortex shows strong immunoreactivity in single oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2887-Immunohistochemistry-NBP2-46617-img0001.jpg)
![Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617] - Staining of mouse cerebral cortex shows strong immunoreactivity in oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2887-Immunohistochemistry-NBP2-46617-img0002.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617] - Staining of human cerebral cortex shows strong immunoreactivity in oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2887-Immunohistochemistry-NBP2-46617-img0003.jpg)
![Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617] - Staining of rat hippocampal formation shows strong immunoreactivity in oligodendrocytes and myelinated fibers.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2887-Immunohistochemistry-NBP2-46617-img0004.jpg)
![Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617] - Staining of mouse amygdala shows strong immunoreactivity in oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2887-Immunohistochemistry-NBP2-46617-img0005.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617] - Staining of human cerebellum shows strong immunoreactivity in oligodendrocytes and myelinated neural fibers.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2887-Immunohistochemistry-NBP2-46617-img0006.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Analysis in human kidney and pancreas tissues using NBP1-80993 antibody. Corresponding SLC12A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-80993-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Analysis in human kidney and pancreas tissues using NBP1-80993 antibody. Corresponding SLC12A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human cerebellum, kidney, liver and pancreas using Anti-SLC12A1 antibody NBP1-80993 (A) shows similar protein distribution across tissues to independent antibody NBP1-82559 (B).](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-80993-img0013.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody [NBP1-80993] - Western blot experiments were performed to determine the protein expression in eight corresponding normal (N) and clear cell renal cell carcinoma (T) tissue. NPTX2 was increased in tumor tissue, whereas FXYD4, KNG1, SLC12A1 and SLC6A3 were decreased in tumors. NDUF4AL2 levels were similar, but the bands were of different size in tumor and normal samples Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/s12943-016-0495-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-80993-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human cell line RPTEC TERT1 shows localization to nucleus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80993-img0007.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-80993-img0012.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-80993-img0014.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-80993-img0015.jpg)
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-80993-img0016.jpg)
![Western Blot: CNPase Antibody (CL2871) [NBP2-46635] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CNP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2871-Western-Blot-NBP2-46635-img0022.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CNPase Antibody (CL2871) [NBP2-46635] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CNP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: CNPase Antibody (CL2871) [NBP2-46635] - Western blot analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2871-Western-Blot-NBP2-46635-img0023.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (CL2871) [NBP2-46635] - Staining of human liver shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2871-Immunohistochemistry-Paraffin-NBP2-46635-img0007.jpg)
![Immunohistochemistry: CNPase Antibody (CL2871) [NBP2-46635] - Staining of rat cerebral cortex shows strong immunoreactivity in oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2871-Immunohistochemistry-NBP2-46635-img0001.jpg)
![Immunohistochemistry: CNPase Antibody (CL2871) [NBP2-46635] - Staining of mouse striatum shows strong positivity in oligodendrocytes and myelinated neural fibers.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2871-Immunohistochemistry-NBP2-46635-img0002.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (CL2871) [NBP2-46635] - Staining of human cerebellum shows strong immunoreactivity in myelinated neural processes.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2871-Immunohistochemistry-Paraffin-NBP2-46635-img0003.jpg)
![Immunohistochemistry: CNPase Antibody (CL2871) [NBP2-46635] - Staining of rat hippocampus shows moderate immunoreactivity in single oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2871-Immunohistochemistry-NBP2-46635-img0004.jpg)
![Immunohistochemistry: CNPase Antibody (CL2871) [NBP2-46635] - Staining of mouse hippocampal formation shows moderate immunoreactivity in single oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2871-Immunohistochemistry-NBP2-46635-img0005.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (CL2871) [NBP2-46635] - Staining of human cerebral cortex shows strong immunoreactivity in oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-CL2871-Immunohistochemistry-Paraffin-NBP2-46635-img0006.jpg)
![Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of skeletal muscle lysates and His-tagged recombinant proteins using mouse parvalbumin mAb, dilution 1:1,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] full length human parvalbumin, [4] full length human calretinin, and [5] full length human calbindin. A band at 12kDa is detected in in muscle lysate and one at 18kDa in the His-tagged recombinant parvalbumin protein lane as expected since the His-tag and other vector derived sequence adds about 6kDa to the molecule. Note that the parvalbumin antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-3C9-Western-Blot-NBP2-50038-img0005.jpg "Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of skeletal muscle lysates and His-tagged recombinant proteins using mouse parvalbumin mAb, dilution 1:1,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] full length human parvalbumin, [4] full length human calretinin, and [5] full length human calbindin. A band at 12kDa is detected in in muscle lysate and one at 18kDa in the His-tagged recombinant parvalbumin protein lane as expected since the His-tag and other vector derived sequence adds about 6kDa to the molecule. Note that the parvalbumin antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences.")
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of rat cerebellum section stained with mouse mAb to parvalbumin, NBP2-50038, dilution 1:1,000, in green, and costained with chicken pAb to calbindin, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone and so appear are green.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-3C9-Immunocytochemistry-Immunofluorescence-NBP2-50038-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038] - Adult rat cerebellum floating section was stained with NBP2-50038 at 1:1,000 (green). Parvalbumin is prominently expressed in the dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-3C9-Immunocytochemistry-Immunofluorescence-NBP2-50038-img0003.jpg)
![Western Blot: CNPase Antibody (JF10-25) [NBP2-66986] - Western blot analysis of CNPase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse brain tissue lysate Lane 2: mouse cerebrum tissue lysate](http://images.novusbio.com/fullsize/CNPase-Antibody-JF10-25-Western-Blot-NBP2-66986-img0008.jpg "Western Blot: CNPase Antibody (JF10-25) [NBP2-66986] - Western blot analysis of CNPase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse brain tissue lysate Lane 2: mouse cerebrum tissue lysate")
![Immunocytochemistry/Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986] - Staining CNPase in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CNPase-Antibody-JF10-25-Immunocytochemistry-Immunofluorescence-NBP2-66986-img0007.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (JF10-25) [NBP2-66986] - Analysis of paraffin-embedded rat brain tissue using anti-CNPase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CNPase-Antibody-JF10-25-Immunohistochemistry-Paraffin-NBP2-66986-img0005.jpg)
![Flow Cytometry: CNPase Antibody (JF10-25) [NBP2-66986] - Analysis of SH-SY-5Y cells with CNPase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CNPase-Antibody-JF10-25-Flow-Cytometry-NBP2-66986-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986] - Staining CNPase in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CNPase-Antibody-JF10-25-Immunocytochemistry-Immunofluorescence-NBP2-66986-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986] - Staining CNPase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CNPase-Antibody-JF10-25-Immunocytochemistry-Immunofluorescence-NBP2-66986-img0003.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (JF10-25) [NBP2-66986] - Analysis of paraffin-embedded mouse brain tissue using anti-CNPase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CNPase-Antibody-JF10-25-Immunohistochemistry-Paraffin-NBP2-66986-img0004.jpg)
![Western Blot: CNPase Antibody (2872) [NBP2-46636] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Western-Blot-NBP2-46636-img0009.jpg "Western Blot: CNPase Antibody (2872) [NBP2-46636] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry-Paraffin: CNPase Antibody (CL2872) [NBP2-46636] - CNPase Antibody (2872) [NBP2-46636] - Staining of human pancreas shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0007.jpg)
![Western Blot: CNPase Antibody (2872) [NBP2-46636] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Western-Blot-NBP2-46636-img0008.jpg)
![Immunohistochemistry: CNPase Antibody (2872) [NBP2-46636] - Staining of rat cerebral cortex shows moderate immunoreactivity in oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0001.jpg)
![Immunohistochemistry: CNPase Antibody (2872) [NBP2-46636] - Staining of mouse striatum shows strong positivity in oligodendrocytes and myelinated neural fibers.](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0002.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (CL2872) [NBP2-46636] - CNPase Antibody (2872) [NBP2-46636] - Staining of human cerebral cortex shows strong immunoreactivity in oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0003.jpg)
![Immunohistochemistry: CNPase Antibody (2872) [NBP2-46636] - Staining of rat cerebral cortex shows strong immunoreactivity in a single oligodendrocyte.](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0004.jpg)
![Immunohistochemistry: CNPase Antibody (2872) [NBP2-46636] - Staining of mouse cerebral cortex shows strong immunoreactivity in oligodendrocytes.](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0005.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (CL2872) [NBP2-46636] - CNPase Antibody (2872) [NBP2-46636] - Staining of human cerebellum shows strong immunoreactivity in oligodendrocytes and myelinated neural processes.](http://images.novusbio.com/fullsize/CNPase-Antibody-2872-Immunohistochemistry-NBP2-46636-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP1-85999] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is show in green.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85999-img0009.jpg "Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP1-85999] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is show in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85999] - Staining in human cerebral cortex and pancreas tissues using NBP1-85999 antibody. Corresponding CNP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-Paraffin-NBP1-85999-img0015.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85999] - Staining of human cerebral cortex shows strong positivity in neuronal processes.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-Paraffin-NBP1-85999-img0011.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85999] - Staining of human duodenum shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-Paraffin-NBP1-85999-img0012.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85999] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-Paraffin-NBP1-85999-img0013.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85999] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-Paraffin-NBP1-85999-img0014.jpg)
