| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-892SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-78486SS | |||
| Azide and BSA Free | NBP2-80884 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-178SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78292SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71134 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73042 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72948 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95105-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73129 | |||
![Western Blot: DLL4 Antibody [NB600-892] - Western Blot of DLL4 antibody. Lane 1: Mouse Pancreas Whole Cell Lysate. Lane 2: HUVEK Whole Cell Lysate.Lane 3: Human rDLL4.Lane 4: HEK293 Whole Cell Lysate + Human rDLL4. Lane 5: HEK293 Whole Cell Lysate.Load: 10 ug per lane for Whole Cell Lysates or 50 ng for recombinant protein.Primary antibody: DLL4 antibody at 1:1,000 overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 74 kDa, 74 kDa for DLL4. Other band(s): Not Identified.](http://images.novusbio.com/fullsize/DLL4-Antibody-Western-Blot-NB600-892-img0006.jpg "Western Blot: DLL4 Antibody [NB600-892] - Western Blot of DLL4 antibody. Lane 1: Mouse Pancreas Whole Cell Lysate. Lane 2: HUVEK Whole Cell Lysate.Lane 3: Human rDLL4.Lane 4: HEK293 Whole Cell Lysate + Human rDLL4. Lane 5: HEK293 Whole Cell Lysate.Load: 10 ug per lane for Whole Cell Lysates or 50 ng for recombinant protein.Primary antibody: DLL4 antibody at 1:1,000 overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 74 kDa, 74 kDa for DLL4. Other band(s): Not Identified.")
![Immunohistochemistry: DLL4 Antibody [NB600-892] - Affinity Purified DLL4 antibody was used at 20 ug/ml to detect DLL4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows DLL4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/DLL4-Antibody-Immunohistochemistry-NB600-892-img0007.jpg)
![Western Blot: DLL4 Antibody [NB600-892] - Western Blot of DLL4 antibody. Lane 1: mouse pancreatic tissue. Load: 20 ug per lane.Primary antibody: DLL4 antibody at 1:500 for overnight at 4C.Secondary antibody: HRP-labeled Goat anti-Rabbit IgG secondary antibody at 1:5,000 for 1 hour at RT. A chemiluminescence system was used for signal detection using a 3 min exposure time.Block: 5% Goat Serum overnight at 4C.Predicted/Observed size: 74 kDa, 70 kDa for Delta 4. Other band(s): none.](http://images.novusbio.com/fullsize/DLL4-Antibody-Western-Blot-NB600-892-img0005.jpg)
![ELISA: DLL4 Antibody [NB600-892] - ELISA results of purified DLL4 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/DLL4-Antibody-ELISA-NB600-892-img0008.jpg)
![Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Knockout-Validated-NB100-78486-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human 270 kDa full-length NOTCH1 and 110-120 kDa cleaved NOTCH 1 (NICD).](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Western-Blot-NB100-78486-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (mN1A) [NB100-78486] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution with DyLight 488 (Green). Actin was counterstained with Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Immunocytochemistry-Immunofluorescence-NB100-78486-img0008.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (mN1A) [NB100-78486] - Analysis of FFPE human pancreatic cancer using 1:10 dilution of Notch-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in epithelial cells was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Immunohistochemistry-Paraffin-NB100-78486-img0010.jpg)
![Flow Cytometry: Notch-1 Antibody (mN1A) [NB100-78486] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Flow-Cytometry-NB100-78486-img0007.jpg)
![Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.](http://images.novusbio.com/fullsize/NUMB-Antibody-Western-Blot-NB500-178-img0005.jpg "Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.")
![Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: NUMB Antibody [NB500-178] - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Numb Antibody (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0007.jpg)
![Simple Western: Numb Antibody [NB500-178] - Simple Western lane view shows a specific band for NUMB in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Numb-Antibody-Simple-Western-NB500-178-img0006.jpg)
![Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17beta-estradiol. Lane 3: MCF-7 + 10 uM gamma secretase inhibitor. Load: 35 ug per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for Notch1.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Western-Blot-NB300-251-img0010.jpg "Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17beta-estradiol. Lane 3: MCF-7 + 10 uM gamma secretase inhibitor. Load: 35 ug per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for Notch1.")
![Immunohistochemistry: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Analysis of Tissue: Exocrine glands of human pancreas Fixation: FFPE Primary antibody: Notch1 antibody at 1:200 Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Immunohistochemistry-NB300-251-img0007.jpg)
![Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Notch 1 Antibody [NB 300-251]- at 1:500, against myc-tagged transiently transfected mouse Notch constructs in 293 cells.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Western-Blot-NB300-251-img0003.jpg)
![Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Western-Blot-NB300-251-img0009.jpg)
![Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Western-Blot-NB300-251-img0011.jpg)
![Western Blot: Notch-1 Antibody [NBP1-78292] - Mouse total protein lysates (50 ug) were separated on 8% SDS-PAGE. Notch-1 (NBP1-78292SS) antibody was used at 1:500 dilution. Actin (1:10,000) was used as normalizer. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP1-78292-img0005.jpg "Western Blot: Notch-1 Antibody [NBP1-78292] - Mouse total protein lysates (50 ug) were separated on 8% SDS-PAGE. Notch-1 (NBP1-78292SS) antibody was used at 1:500 dilution. Actin (1:10,000) was used as normalizer. WB image submitted by a verified customer review.")
![Immunohistochemistry: Notch-1 Antibody [NBP1-78292] - Staining of Notch1 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunohistochemistry-NBP1-78292-img0003.jpg)
![Western Blot: Notch-1 Antibody [NBP1-78292] - Analysis of Cleaved Notch1 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP1-78292-img0002.jpg)
![Simple Western: Notch-1 Antibody [NBP1-78292] - Lane view shows a specific band for Notch-1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Simple-Western-NBP1-78292-img0004.jpg)
![Western Blot: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Western-Blot-NBP2-01926-img0022.jpg "Western Blot: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.")
![Immunocytochemistry/Immunofluorescence: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM1.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunocytochemistry-NBP2-01926-img0024.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human prostate tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0021.jpg)
![Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - Analysis of Jurkat cells, using anti-LHX1 antibody (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Flow-Cytometry-NBP2-01926-img0025.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0012.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0019.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human Kidney tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0014.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human liver tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0015.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human pancreas tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0020.jpg)
![Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIM1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Flow-Cytometry-NBP2-01926-img0023.jpg)
![Western Blot: Notch-1 Antibody (3E12) [NBP1-48289] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Western-Blot-NBP1-48289-img0030.jpg "Western Blot: Notch-1 Antibody (3E12) [NBP1-48289] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1.")
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of HepG2 cells using anti Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunocytochemistry-Immunofluorescence-NBP1-48289-img0032.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded thyroid using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunocytochemistry-Immunofluorescence-NBP1-48289-img0016.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0017.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0018.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded breast using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0019.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0020.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of lung using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0021.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0022.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0023.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded colon using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0024.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded endometrium using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0025.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded kidney using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0026.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded lung using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0027.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded pancreas using anti-Notch1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Notch-1-Antibody-3E12-Immunohistochemistry-Paraffin-NBP1-48289-img0028.jpg)
![Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Neurogenin 1 monoclonal antibody.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Western-Blot-NBP1-47871-img0020.jpg "Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Neurogenin 1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - COS7 cells transiently transfected by pCMV6-ENTRY Neurogenin-1.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunocytochemistry-Immunofluorescence-NBP1-47871-img0011.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human prostate tissue.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunohistochemistry-Paraffin-NBP1-47871-img0017.jpg)
![Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neurogenin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Neurogenin 1.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Western-Blot-NBP1-47871-img0019.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human colon tissue.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunohistochemistry-Paraffin-NBP1-47871-img0012.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human Kidney tissue.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunohistochemistry-Paraffin-NBP1-47871-img0013.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human lung tissue.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunohistochemistry-Paraffin-NBP1-47871-img0014.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human Ovary tissue.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunohistochemistry-Paraffin-NBP1-47871-img0015.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human pancreas tissue.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunohistochemistry-Paraffin-NBP1-47871-img0016.jpg)
![Immunoprecipitation: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Immunoprecipitation (IP) of NEUROG1 by using TrueMab monoclonal anti-NEUROG1 antibodies (Negative control: IP without adding anti-NEUROG1 antibody.). For each experiment, 500ul of DDK tagged NEUROG1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/Neurogenin-1-Antibody-OTI3F9-Immunoprecipitation-NBP1-47871-img0021.jpg)
![Western Blot: NUMB Antibody [NBP2-27181] - Analysis of mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NUMB antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution were used for this test](http://images.novusbio.com/fullsize/NUMB-Antibody-Western-Blot-NBP2-27181-img0004.jpg "Western Blot: NUMB Antibody [NBP2-27181] - Analysis of mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NUMB antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution were used for this test")
![Immunohistochemistry-Paraffin: NUMB Antibody [NBP2-27181] - Formalin-fixed, paraffin-embedded human adrenal tissue stained with NUMB antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunohistochemistry-Paraffin-NBP2-27181-img0006.jpg)
![Immunohistochemistry-Paraffin: NUMB Antibody [NBP2-27181] - Formalin-fixed, paraffin-embedded human spleen stained with NUMB antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunohistochemistry-Paraffin-NBP2-27181-img0005.jpg)
![Western Blot: Notch-1 Antibody [NBP2-95105] - Analysis of extracts of U-251MG cells, using Notch-1 .Exposure time: 300s.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP2-95105-img0006.jpg "Western Blot: Notch-1 Antibody [NBP2-95105] - Analysis of extracts of U-251MG cells, using Notch-1 .Exposure time: 300s.")
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251638038.jpg)
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-522025163964.jpg)
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251638034.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of U2OS cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251639698.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of HeLa cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251636390.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of A-549 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-52202516283218.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of PC-12 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251638067.jpg)
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-522025163633.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of NIH/3T3 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-52202516345270.jpg)
![Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Western-Blot-H00004760-M01-img0011.jpg "Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Immunocytochemistry-Immunofluorescence-H00004760-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Immunohistochemistry-Paraffin-H00004760-M01-img0009.jpg)
![Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Western Blot analysis of NEUROD1 expression in IMR-32 ( Cat # L008V1 ).](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Western-Blot-H00004760-M01-img0001.jpg)
![ELISA: NeuroD1 Antibody (3H8) [H00004760-M01] - Detection limit for recombinant GST tagged NEUROD1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-ELISA-H00004760-M01-img0012.jpg)
![Western Blot: Numb Antibody (4F3) [NBP2-45877] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB .](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Western-Blot-NBP2-45877-img0017.jpg "Western Blot: Numb Antibody (4F3) [NBP2-45877] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB .")
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0016.jpg)
![Western Blot: Numb Antibody (4F3) [NBP2-45877] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Western-Blot-NBP2-45877-img0018.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0001.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human lung tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0002.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0003.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0004.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0005.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0006.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0007.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0008.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0009.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0010.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human prostate tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0011.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0012.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0013.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0014.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human bladder tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0015.jpg)