| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-157SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-42827SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05423-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25170SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2216SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32932-0.02mg | |||
| Azide and BSA Free | NBP2-34713-0.2mg | |||
| Azide and BSA Free | NBP2-34713-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-675SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-488SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-676SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56351SS | |||
![Immunocytochemistry/Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 1:50 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Somatostatin-R2-SSTR2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-157-img0005.jpg "Immunocytochemistry/Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 1:50 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157] - Staining of Somatostatin Receptor 2 in mouse pancreas using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Somatostatin-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NB300-157-img0004.jpg)
![Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157] - Staining of paraffin embedded rat pancreas tissue.](http://images.novusbio.com/fullsize/Somatostatin-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NB300-157-img0003.jpg)
![Western Blot: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Analysis of phosphorylated MAP1B in chick embryos (lane 1) and rat neonatal brain (lane 2).](http://images.novusbio.com/fullsize/MAP1B-[p-Thr1265]-Antibody---SuperBUGS-Western-Blot-NBP1-42827-img0003.jpg "Western Blot: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Analysis of phosphorylated MAP1B in chick embryos (lane 1) and rat neonatal brain (lane 2).")
![Immunohistochemistry: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Immunohistochemical analysis of rat spinal cord transverse section from an E12 embryo.](http://images.novusbio.com/fullsize/MAP1B-[p-Thr1265]-Antibody---SuperBUGS-Immunohistochemistry-NBP1-42827-img0004.jpg)
![MAP1B-[p-Thr1265]-Antibody-SuperBUGS-Western-Blot-NBP1-42827-img0005.jpg](http://images.novusbio.com/fullsize/MAP1B-[p-Thr1265]-Antibody-SuperBUGS-Western-Blot-NBP1-42827-img0005.jpg)
![Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of Parkin on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Western-Blot-NBP2-67017-img0007.jpg "Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of Parkin on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded rat brain tissue using anti-Parkin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunohistochemistry-Paraffin-NBP2-67017-img0004.jpg)
![Flow Cytometry: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of SH-SY-5Y cells with Parkin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Flow-Cytometry-NBP2-67017-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0006.jpg)
![Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded mouse testis tissue using anti-Parkin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunohistochemistry-Paraffin-NBP2-67017-img0003.jpg)
![Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] -Analysis of Parkin on different lysates with Rabbit anti-Parkin antibody at 1/1,000 dilution.Lane 1: 293 cell lysateLane 2: Jurkat cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 52 kDaObserved band size: 52 kDaExposure time: 1 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67017_rabbit-monoclonal-parkin-antibody-jf82-09-western-blot-1962024104114..jpg)
![Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Western-Blot-NBP1-05423-img0003.jpg "Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - Analysis of mixed fibroblast and PC12 pheochromocytoma cell culture stained with chicken pAb to peripherin, NBP1-05423, dilution 1:5,000 in green, and costained with rabbit pAb to vimentin, RPCA-Vim, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. NBP1-05423 antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05423-img0005.jpg)
![Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (red) in nerve bundles in the human larynx. Image from verified customer review.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Frozen-NBP1-05423-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - shows rat mixed neuron/glial cultures stained with NBP1-05423, our chicken polyclonal antibody to peripherin (green channel) and rabbit antibody to the neurofilament subunit alpha-internexin NBP1-05207 (green channel). These cultures contain mostly neurons which are rich in alpha-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of peripherin and alpha-internexin, the green and red signals superimpose to produce a golden cell. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05423-img0001.jpg)
![Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (green) in the mouse brainstem including the spinal trigeminal tract (right side of image) and arching fiber bundle to the solitary nuclear tract (center). Nissl Counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Frozen-NBP1-05423-img0006.jpg)
![Western Blot: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in (A) human brain lysate and (B) mouse brain lysate.](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-Western-Blot-NBP2-25170-img0001.jpg "Western Blot: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in (A) human brain lysate and (B) mouse brain lysate.")
![Immunocytochemistry/Immunofluorescence: Synaptophysin Antibody [NBP2-25170] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Synaptophysin at a 1:50 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25170-img0003.jpg)
![Immunohistochemistry: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in mouse brain.](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-Immunohistochemistry-NBP2-25170-img0002.jpg)
![Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Analysis of ApoER2 on mouse brain membrane.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Western-Blot-NB100-2216-img0005.jpg "Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Analysis of ApoER2 on mouse brain membrane.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2216-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) [NBP2-32932] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD34 MAb (QBEnd/10)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Immunohistochemistry-Paraffin-NBP2-32932-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) [NBP2-32932] - Formalin-paraffin human tonsil (20X) stained with CD34 MAb (QBEnd/10)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Immunohistochemistry-Paraffin-NBP2-32932-img0002.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NB300-675] - Analysis of extracts from C6 cells using PGP9.5 / UCHL-1 antibody (NB300-675, 1:200). Image from verified customer review.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB300-675-img0012.jpg "Western Blot: UCH-L1/PGP9.5 Antibody [NB300-675] - Analysis of extracts from C6 cells using PGP9.5 / UCHL-1 antibody (NB300-675, 1:200). Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-675] - Analysis of acetone-fixed, frozen mouse ear skin section using anti-PGP9.5 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunofluorescence-NB300-675-img0014.jpg)
![Immunohistochemistry: PGP9.5 / UCHL-1 Antibody [NB300-675] - Human brain, Substantia Nigra, Pigmented Neuron, 40X.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunohistochemistry-NB300-675-img0010.jpg)
![Western Blot: PGP9.5 / UCHL-1 Antibody [NB300-675] - Detection of UCHL1 in mouse brain lysate.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB300-675-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-675] - In Neuro2a cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-675-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-675] - Confocal analysis of C6 cells using PGP9.5 / UCHL-1 antibody (NB300-675, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-675-img0013.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Western-Blot-NB110-58870-img0007.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.")
![Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of mixed cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:500 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. the NSE antibody labels protein expressed in neuronal cells, while the GFAP antibody stains intermediate filaments in astrocytic and certain other glial cells.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58870-img0006.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Western-Blot-NB110-58870-img0005.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Western blot analysis of extracts from mouse brain using NSE antibody (NB110-58870, 1:100).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Western-Blot-NB110-58870-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Shows the human embryonic kidney cells line 293, which express many neuronal proteins (1). The red channel shows staining which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker to UCHL1 using NB110-58869. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58870-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Confocal immunofluorescent analysis of 3T3 cells using NSE antibody (NB110-58870, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58870-img0003.jpg)
![Simple Western: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Simple Western lane view shows a specific band for Enolase 2/ Neuron specific enolase in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Simple-Western-NB110-58870-img0004.jpg)
![Western Blot: ZIC1 Antibody [NB600-488] - WB analysis of Zic1 in overexpression lysate.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Western-Blot-NB600-488-img0003.jpg "Western Blot: ZIC1 Antibody [NB600-488] - WB analysis of Zic1 in overexpression lysate.")
![Western Blot: ZIC1 Antibody [NB600-488] - Western blot analysis of Zic1 in mouse cerebellum extract. Protein extracts were prepared from mouse cerebellum between postnatal day 5 (P5) and P18, as indicated above the lanes.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Western-Blot-NB600-488-img0001.jpg)
![Immunohistochemistry: ZIC1 Antibody [NB600-488] - IHC analysis of Zic1 in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Immunohistochemistry-NB600-488-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Altered brain pericyte proliferation in Foxc1 mutants.(A–C) PDGFr beta (green) & IB4 (red) immunofluorescence highlight pericyte cell bodies in the cortices of E14.5 WT, Foxc1h/h & Foxc1l/l. (D–F) Triple immunofluorescence for Zic1 (pericyte nuclei; green), BrdU (red) & IB4 (blue) highlight proliferating (Zic1+/BrdU+; arrows) pericytes in the E14.5 cerebral vasculature of WT, Foxc1h/h & Foxc1l/l embryos. (G) Graph depicting quantification of Zic1+ pericyte nuclei/vessel length in all three genotypes at E14.5. (H) Graph depicting quantification of pericyte proliferation (labeling index) at E14.5. Scale bars: 100 µm. Means represent analysis of three independent litters for each mutant genotype (n = 3). Asterisks indicate a statistically significance difference from WT (P<0.05). Error bars depict ±SEM. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416163796.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Altered brain pericyte proliferation in Foxc1 mutants.(A–C) PDGFr beta (green) & IB4 (red) immunofluorescence highlight pericyte cell bodies in the cortices of E14.5 WT, Foxc1h/h & Foxc1l/l. (D–F) Triple immunofluorescence for Zic1 (pericyte nuclei; green), BrdU (red) & IB4 (blue) highlight proliferating (Zic1+/BrdU+; arrows) pericytes in the E14.5 cerebral vasculature of WT, Foxc1h/h & Foxc1l/l embryos. (G) Graph depicting quantification of Zic1+ pericyte nuclei/vessel length in all three genotypes at E14.5. (H) Graph depicting quantification of pericyte proliferation (labeling index) at E14.5. Scale bars: 100 µm. Means represent analysis of three independent litters for each mutant genotype (n = 3). Asterisks indicate a statistically significance difference from WT (P<0.05). Error bars depict ±SEM. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416175243.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation & density in the cerebral vasculature.(A,C) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. (B,D) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. Arrowheads indicate PDGFr beta + pericytes. (E,F) Zic1 (green), BrdU (red), & IB4 (blue) triple immunofluorescence of E14.5 PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. (G) Graph depicts quantification of Zic1+ pericyte proliferation (labeling index) in the cerebral vasculature at E14.5. (H,J) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. (I,K) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. Arrowheads indicate PDGFr beta + pericytes. (L) Graph depicts pericyte density (Zic1+ pericyte nuclei/vessel length) in the cerebral vasculature at E14.5 & E18.5 in PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ animals. Asterisks indicate a statistically significance difference from PDGFr beta -cre; Foxc1fl-+ samples (P<0.05). Analysis of pericyte proliferation & density was performed on control & mutant brains from three separate litters at each time point (n = 3). Error bars depict ±SEM. Scale bars in A,C,E,F,H,J: 100 µm. Scale bars in B,D,I,K: 50 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416171429.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation & density in the cerebral vasculature.(A,C) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. (B,D) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. Arrowheads indicate PDGFr beta + pericytes. (E,F) Zic1 (green), BrdU (red), & IB4 (blue) triple immunofluorescence of E14.5 PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. (G) Graph depicts quantification of Zic1+ pericyte proliferation (labeling index) in the cerebral vasculature at E14.5. (H,J) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. (I,K) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. Arrowheads indicate PDGFr beta + pericytes. (L) Graph depicts pericyte density (Zic1+ pericyte nuclei/vessel length) in the cerebral vasculature at E14.5 & E18.5 in PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ animals. Asterisks indicate a statistically significance difference from PDGFr beta -cre; Foxc1fl-+ samples (P<0.05). Analysis of pericyte proliferation & density was performed on control & mutant brains from three separate litters at each time point (n = 3). Error bars depict ±SEM. Scale bars in A,C,E,F,H,J: 100 µm. Scale bars in B,D,I,K: 50 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416161818.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation & density in the cerebral vasculature.(A,C) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. (B,D) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. Arrowheads indicate PDGFr beta + pericytes. (E,F) Zic1 (green), BrdU (red), & IB4 (blue) triple immunofluorescence of E14.5 PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. (G) Graph depicts quantification of Zic1+ pericyte proliferation (labeling index) in the cerebral vasculature at E14.5. (H,J) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. (I,K) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. Arrowheads indicate PDGFr beta + pericytes. (L) Graph depicts pericyte density (Zic1+ pericyte nuclei/vessel length) in the cerebral vasculature at E14.5 & E18.5 in PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ animals. Asterisks indicate a statistically significance difference from PDGFr beta -cre; Foxc1fl-+ samples (P<0.05). Analysis of pericyte proliferation & density was performed on control & mutant brains from three separate litters at each time point (n = 3). Error bars depict ±SEM. Scale bars in A,C,E,F,H,J: 100 µm. Scale bars in B,D,I,K: 50 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416171415.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation & density in the cerebral vasculature.(A,C) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. (B,D) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. Arrowheads indicate PDGFr beta + pericytes. (E,F) Zic1 (green), BrdU (red), & IB4 (blue) triple immunofluorescence of E14.5 PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. (G) Graph depicts quantification of Zic1+ pericyte proliferation (labeling index) in the cerebral vasculature at E14.5. (H,J) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. (I,K) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. Arrowheads indicate PDGFr beta + pericytes. (L) Graph depicts pericyte density (Zic1+ pericyte nuclei/vessel length) in the cerebral vasculature at E14.5 & E18.5 in PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ animals. Asterisks indicate a statistically significance difference from PDGFr beta -cre; Foxc1fl-+ samples (P<0.05). Analysis of pericyte proliferation & density was performed on control & mutant brains from three separate litters at each time point (n = 3). Error bars depict ±SEM. Scale bars in A,C,E,F,H,J: 100 µm. Scale bars in B,D,I,K: 50 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-31020241616049.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation & density in the cerebral vasculature.(A,C) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. (B,D) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. Arrowheads indicate PDGFr beta + pericytes. (E,F) Zic1 (green), BrdU (red), & IB4 (blue) triple immunofluorescence of E14.5 PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. (G) Graph depicts quantification of Zic1+ pericyte proliferation (labeling index) in the cerebral vasculature at E14.5. (H,J) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. (I,K) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. Arrowheads indicate PDGFr beta + pericytes. (L) Graph depicts pericyte density (Zic1+ pericyte nuclei/vessel length) in the cerebral vasculature at E14.5 & E18.5 in PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ animals. Asterisks indicate a statistically significance difference from PDGFr beta -cre; Foxc1fl-+ samples (P<0.05). Analysis of pericyte proliferation & density was performed on control & mutant brains from three separate litters at each time point (n = 3). Error bars depict ±SEM. Scale bars in A,C,E,F,H,J: 100 µm. Scale bars in B,D,I,K: 50 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416154640.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation & density in the cerebral vasculature.(A,C) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. (B,D) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. Arrowheads indicate PDGFr beta + pericytes. (E,F) Zic1 (green), BrdU (red), & IB4 (blue) triple immunofluorescence of E14.5 PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. (G) Graph depicts quantification of Zic1+ pericyte proliferation (labeling index) in the cerebral vasculature at E14.5. (H,J) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. (I,K) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. Arrowheads indicate PDGFr beta + pericytes. (L) Graph depicts pericyte density (Zic1+ pericyte nuclei/vessel length) in the cerebral vasculature at E14.5 & E18.5 in PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ animals. Asterisks indicate a statistically significance difference from PDGFr beta -cre; Foxc1fl-+ samples (P<0.05). Analysis of pericyte proliferation & density was performed on control & mutant brains from three separate litters at each time point (n = 3). Error bars depict ±SEM. Scale bars in A,C,E,F,H,J: 100 µm. Scale bars in B,D,I,K: 50 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416182612.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Altered brain pericyte proliferation in Foxc1 mutants.(A–C) PDGFr beta (green) & IB4 (red) immunofluorescence highlight pericyte cell bodies in the cortices of E14.5 WT, Foxc1h/h & Foxc1l/l. (D–F) Triple immunofluorescence for Zic1 (pericyte nuclei; green), BrdU (red) & IB4 (blue) highlight proliferating (Zic1+/BrdU+; arrows) pericytes in the E14.5 cerebral vasculature of WT, Foxc1h/h & Foxc1l/l embryos. (G) Graph depicting quantification of Zic1+ pericyte nuclei/vessel length in all three genotypes at E14.5. (H) Graph depicting quantification of pericyte proliferation (labeling index) at E14.5. Scale bars: 100 µm. Means represent analysis of three independent litters for each mutant genotype (n = 3). Asterisks indicate a statistically significance difference from WT (P<0.05). Error bars depict ±SEM. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-31020241616060.jpg)
![Knockout Validated: UCH-L1/PGP9.5 Antibody [NB300-676] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and UCH-L1/PGP9.5 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human UCH-L1/PGP9.5 Polyclonal Antibody (Catalog # NB300-676) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UCH-L1/PGP9.5 at approximately 28 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Knockout-Validated-NB300-676-img0013.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: UCH-L1/PGP9.5 Antibody [NB300-676] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and UCH-L1/PGP9.5 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human UCH-L1/PGP9.5 Polyclonal Antibody (Catalog # NB300-676) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UCH-L1/PGP9.5 at approximately 28 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: UCH-L1/PGP9.5 Antibody [NB300-676] - Analysis of UCHL1 in mouse brain lysate ECL detection, 60 seconds.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB300-676-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-676] - Neuro2a cells stained with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-676-img0007.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NB300-676] - Staining of neurons and cell processes at 2.5ug/mL. Human brain, Substantia Nigra, Pigmented Neurons, 4X.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunohistochemistry-Paraffin-NB300-676-img0009.jpg)
![Flow Cytometry: UCH-L1/PGP9.5 Antibody [NB300-676] - An intracellular stain was performed on U-87 cells with UCH-L1/PGP9.5 Antibody NB300-676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Flow-Cytometry-NB300-676-img0012.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody [NB300-676] - Image shows a specific band for PGP9.5/UCHL-1 in 0.05 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Simple-Western-NB300-676-img0011.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-56351] - Analysisof 5-HT3A in human testis using 5-HT3A antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Immunohistochemistry-Paraffin-NB100-56351-img0010.jpg)
![Western Blot: 5-HT3A Antibody [NB100-56351] - Analysis of 5-HT3A in A: human, B: mouse and C: rat brain tissue lysate using 5-HT3A antibody at 3 ug/mL.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Western-Blot-NB100-56351-img0012.jpg)
![Simple Western: 5-HT3A Antibody [NB100-56351] - Image shows a specific band for 5HT3A in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Simple-Western-NB100-56351-img0007.jpg)
