| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-147SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31376SS | |||
| Azide and BSA Free | NBP2-80741 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13249-0.2mg | |||
| BSA Free | NB120-13249-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74229 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58771SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78162SS | |||
| Azide and BSA Free | NBP2-81010 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-137-0.025ml | |||
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Western-Blot-NB300-147-img0005.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.")
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Sodium Potassium ATPase Beta 1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Immunocytochemistry-Immunofluorescence-NB300-147-img0008.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Detection of Na, K-ATPase (beta) in rat kidney homogenates (20 ug)using NB 300-147. Lane 1: 1:2,500 primary. Lane 2: 1:5,000 primary.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Western-Blot-NB300-147-img0007.jpg)
![Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Western-Blot-NBP2-31376-img0007.jpg "Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Immunocytochemistry-Immunofluorescence-NBP2-31376-img0009.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as nuclei, whereas the stromal cells showed mild cytoplasmic staining only.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Immunohistochemistry-Paraffin-NBP2-31376-img0011.jpg)
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Flow-Cytometry-NBP2-31376-img0016.jpg)
![Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Western-Blot-NBP2-31376-img0004.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and nuclear positivity was observed in the cells of various tubules/ducts of renal medulla.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Immunohistochemistry-Paraffin-NBP2-31376-img0010.jpg)
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary CD4+ T-cells isolated from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Flow-Cytometry-NBP2-31376-img0013.jpg)
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Flow-Cytometry-NBP2-31376-img0014.jpg)
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Flow-Cytometry-NBP2-31376-img0015.jpg)
![Simple Western: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Simple-Western-NBP2-31376-img0012.jpg)
![Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat brain tissue lysate.](http://images.novusbio.com/fullsize/Bassoon-Antibody-SAP7F407-Western-Blot-NB120-13249-img0001.jpg "Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat brain tissue lysate.")
![Immunocytochemistry/Immunofluorescence: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat Hippocampal Neuron, image courtesy of customer.](http://images.novusbio.com/fullsize/Bassoon-Antibody-SAP7F407-Immunocytochemistry-Immunofluorescence-NB120-13249-img0003.jpg)
![Immunohistochemistry: Bassoon Antibody (SAP7F407) [NB120-13249] - Bassoon localization in the rat brain cerebellar molecular layer using Bassoon Monoclonal Antibody.](http://images.novusbio.com/fullsize/Bassoon-Antibody-SAP7F407-Immunohistochemistry-NB120-13249-img0002.jpg)
![Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.](http://images.novusbio.com/fullsize/NF-M-Antibody-NB300-222-img0004.jpg "Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-222] - 160kDa Neurofilament Medium Antibody [NB300-222] - View of mixed neuron/glial cultures stained with NB300-222 (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.](http://images.novusbio.com/fullsize/160kDa-Neurofilament-Medium-Antibody-Immunocytochemistry-Immunofluorescence-NB300-222-img0002.jpg)
![Immunohistochemistry Free-Floating: NF-M Antibody [NB300-222] - Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, NB300-222, dilution 1:1,000 in red, and costained with mouse mAb to CNPase, dilution 1:500, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. The NF-M antibody labels the axons of basket cells and other neurons, while the CNP antibody stains oligodendrocytes, cells that form the myelin sheathes around axons.](http://images.novusbio.com/fullsize/NF-M-Antibody-NB300-222-img0003.jpg)
![Western Blot: Ubiquitin Antibody [NB300-129] - Analysis of HEK293 cell lysates using rabbit Ubiquitin antibody, dilution 1:5000 (Green), and mouse beta-Tubulin antibody, dilution 1:10000 (Red) used as a loading control. [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. Lysed cells were lysed and the lysate subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8kDa corresponds to monoubiquitin.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Western-Blot-NB300-129-img0009.jpg "Western Blot: Ubiquitin Antibody [NB300-129] - Analysis of HEK293 cell lysates using rabbit Ubiquitin antibody, dilution 1:5000 (Green), and mouse beta-Tubulin antibody, dilution 1:10000 (Red) used as a loading control. [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. Lysed cells were lysed and the lysate subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8kDa corresponds to monoubiquitin.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NB300-129] - Analysis of HeLa cells stained with rabbit pAb to ubiquitin, NB300-129, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. [A] Control HeLa cells maintained in normal medium, [B] HeLa cells treated with 10uM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions. Note the diffuse cytoplasmic ubiquitin staining in control cells and well defined ubiquitin positive inclusions in the Lc treated cells.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-129-img0008.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody [NB300-129] - IHC staining of Ubiquitin in human rectal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunohistochemistry-Paraffin-NB300-129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NB300-129] - Immunofluorescent staining of aggresomes of mouse L-cells treated with lactacysteine, using NB300-129.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-129-img0001.jpg)
![Western Blot: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLUG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLUG.](http://images.novusbio.com/fullsize/SLUG-Antibody-1A6-Western-Blot-NBP2-03886-img0002.jpg "Western Blot: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLUG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLUG.")
![Immunohistochemistry-Paraffin: Slug Antibody (1A6) [NBP2-03886] - Mouse glioma stained with Slug antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Slug-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0009.jpg)
![Flow Cytometry: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLUG antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SLUG-Antibody-1A6-Flow-Cytometry-NBP2-03886-img0001.jpg)
![Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SLUG mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLUG-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0003.jpg)
![Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SLUG mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLUG-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0004.jpg)
![Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SLUG mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLUG-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0005.jpg)
![Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SLUG mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLUG-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0006.jpg)
![Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Human Kidney tissue using anti-SLUG mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLUG-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0007.jpg)
![Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] Staining of paraffin-embedded Human lymphoma tissue using anti-SLUG mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLUG-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0008.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood lymphocytes with anti-human CD45 antibody (MEM-28) Alexa Fluor 700.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0021.jpg "Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood lymphocytes with anti-human CD45 antibody (MEM-28) Alexa Fluor 700.")
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (MEM-28) [NB500-319] - staining of human peripheral blood mononuclear cell using anti-human CD45 antibody (MEM-28) (green). DNA visualized by DAPI (blue).](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Immunocytochemistry-NB500-319-img0001.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE-CyTM5.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0018.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (MEM-28) [NB500-319] - Staining of human small intestine (paraffin-embedded sections) with CD45 antibody (MEM-28), 10 ug/ml.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Immunohistochemistry-Paraffin-NB500-319-img0022.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0007.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) purified, GAM-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0008.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) APC-CyTM7.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0010.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0016.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0017.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE-CyTM7.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0019.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE-DyLight (R) 594.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0020.jpg)
![Western Blot: LRRK2 Antibody [NB110-58771] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LRRK2 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human LRRK2 Polyclonal Antibody (Catalog # NB110-58771) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for LRRK2 at approximately 275 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Knockout-Validated-NB110-58771-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LRRK2 Antibody [NB110-58771] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LRRK2 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human LRRK2 Polyclonal Antibody (Catalog # NB110-58771) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for LRRK2 at approximately 275 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: LRRK2 Antibody [NB110-58771] - NB110-58771 in increasing amounts of human lymphoblast](http://images.novusbio.com/fullsize/LRRK2-Antibody-Western-Blot-NB110-58771-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB110-58771] - Immunofluorescence of Lrrk2 with NB110-58771 within (A) Substantia nigra neurons (B) Chains of migrating neuroblasts.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58771-img0008.jpg)
![Immunohistochemistry-Frozen: LRRK2 Antibody [NB110-58771] - DAB Immunostaining in frozen sections of mouse brainstem.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunohistochemistry-NB110-58771-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: LRRK2 Antibody [NB110-58771] - LRRK2 RNA levels 5 days after shRNA lentiviral transduction. The empty vector was used as the mock control. Lines represent the mean +/- SEM of three to four independent silencing experiments. A representative immunoblot analysis of LRRK2 protein levels 5 days after transduction is also shown. Beta-III-tubulin was the loading control. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0761-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Knockdown-Validated-NB110-58771-img0010.jpg)
![Western Blot: LRRK2 Antibody [NB110-58771] - Characterization of iPSC-derived DA neurons with LRRK2 mutations. a Diagram showing the DA differentiation protocol used for neural induction of human iPSC lines. b Temporal gene expression analyzed by qRT-PCR at three time points: induction (3 weeks), expansion (4–5 weeks), & maturation (>6 weeks). Each point represents the mean ± SEM of at least two independent differentiation experiments. c Representative images of mature neuronal cultures showing expression of neuronal ( beta III-tubulin, Tau, & alpha -synuclein) & dopaminergic (TH, NURR1) markers. Nuclei were counterstained with Hoechst. Scale bars: 50 μm. d Quantification of immunostainings. Data are represented as mean ± SEM of counts from at least two different lines for each genotype. e Representative western blot analyses of TH, Tau, & GFAP with beta III-tubulin as loading control in iPSC-derived mature neurons. f Representative immunoblots & quantification of LRRK2 expression in mature neuronal cultures. alpha -tubulin was the loading control & data were normalized to control WT neurons. Bars represent the mean ± SEM of at least two different lines per genotype. DIV days in vitro, GEL gelatin, POL poly-ornithine, FBN fibronectin, LMN laminin, N2 N2 supplement, bFGF basic fibroblast growth factor, SAG smoothened agonist, LDN LDN-193189, CHIR CHIR99021, SB SB431542, BDNF brain-derived neurotrophic factor, AA ascorbic acid, B27 B27 supplement, dbcAMP dibutyryl cyclic adenosine monophosphate, TGF beta III transforming growth factor beta III, GDNF glial derived neurotrophic factor. See Additional file 2 for uncropped blots Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0761-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58771_rabbit-polyclonal-lrrk2-antibody-31020241623559.jpg)
![Western Blot: VLDLR Antibody (6A6) [NBP1-78162] - MCF-7 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. VLDLR Antibody (NBP1-78162) antibody at 1:1000, 4C, overnight. WB image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/VLDLR-Antibody-6A6-Western-Blot-NBP1-78162-img0010.jpg "Western Blot: VLDLR Antibody (6A6) [NBP1-78162] - MCF-7 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. VLDLR Antibody (NBP1-78162) antibody at 1:1000, 4C, overnight. WB image submitted by a verifed customer review.")
![Immunocytochemistry/Immunofluorescence: VLDLR Antibody (6A6) [NBP1-78162] - VLDL R Antibody (6A6) [NBP1-78162] - VLDL Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/VLDL-R-Antibody-6A6-Immunocytochemistry-Immunofluorescence-NBP1-78162-img0006.jpg)
![Immunohistochemistry: VLDL R Antibody (6A6) [NBP1-78162] - Analysis of VLDL Receptor in mouse kidney.](http://images.novusbio.com/fullsize/VLDL-R-Antibody-6A6-Immunohistochemistry-NBP1-78162-img0007.jpg)
![Western Blot: VLDL R Antibody (6A6) [NBP1-78162] - Analysis of VLDL Receptor expression in mouse kidney.](http://images.novusbio.com/fullsize/VLDL-R-Antibody-6A6-Western-Blot-NBP1-78162-img0008.jpg)
![Western Blot: Peripherin Antibody [NB300-137] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:10000 in green: [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at ~57kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Western-Blot-NB300-137-img0004.jpg "Western Blot: Peripherin Antibody [NB300-137] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:10000 in green: [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at ~57kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NB300-137] - Analysis of the widely used rat pheochromocytoma PC12 cell line stained with rabbit polyclonal Periphren antibody, dilution 1:2000 (Green). Hoechst staining of nuclear DNA (Blue). Peripherin, one of the Class III family of intermediate filament subunit proteins, is a major component of the PC12 cell forming a perinuclear cap, with some filaments visible in the cytoplasm.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-137-img0003.jpg)
![Western Blot: Peripherin Antibody [NB300-137] Detection of the 57 kDa peripherin protein in whole rat brain stem homogenate using NB 300-137 (1:20,000). The faint 48 kDa band is a peripherin molecule.](http://images.novusbio.com/fullsize/p_4356adfdef9d7.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NB300-137] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-137 antibody to peripherin (green) and chicken polyclonal antibody to phosphorylated NF-H (red channel). A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain NF-H and not peripherin. The blue channel reveals the localization of DNA.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-137-img0001.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Mouse whole brain lysate showing specific immunolabeling of the ~46 kDa gamma-2-subunit of GABAa -R.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Western-Blot-NB300-190-img0002.jpg "Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Mouse whole brain lysate showing specific immunolabeling of the ~46 kDa gamma-2-subunit of GABAa -R.")
![Immunohistochemistry: GABA-A R gamma 2 Antibody [NB300-190] - Immunostaining of mouse retina showing specific labeling of the GABAa gamma-2 subunit in green, calbindin in red and DNA in blue. Photo courtesy of Dr. Arlene Hirano, UCLA.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-190-img0001.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Pattern of YFP gamma 2 expression in retinas of Thy1-YFP gamma 2 transgenic mice.(A) Maximum intensity projections (MIP) of confocal image stacks (2 µm total thickness) of vertical slices of Thy1-YFP gamma 2 retinas at postnatal day (P)12 & P21. Diffuse fluorescence expression in cell bodies within the ganglion cell layer (GCL) at both ages, & punctate expression throughout both OFF & ON layers of the inner plexiform layer (IPL) are apparent. There was no expression in the outer plexiform layer (OPL) or the outer nuclear layer (ONL) at either age. (B) Images of MIPs (∼20 µm thick) at various depths of a P21 flat mount Thy1-YFP gamma 2 retina. Inserts are 3× magnification of the images. (C) Western blot of whole brain lysates from Thy1-YFP gamma 2 (Tg) & wildtype (WT) mice. YFP gamma 2 (arrowhead) was detected with anti-GABAAR gamma 2 (upper) & anti-GFP (lower). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-31020241620510.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212325.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212373.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-31020241621817.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212319.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416205172.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416205153.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212384.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212327.jpg)
![Immunocytochemistry/Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (Green) P0 concentration was 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). Red is actin. Blue is DAPI nuclear counterstain.](http://images.novusbio.com/fullsize/Myelin-Protein-Zero-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1607-img0001.jpg "Immunocytochemistry/Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (Green) P0 concentration was 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). Red is actin. Blue is DAPI nuclear counterstain.")
![Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607] - In the image (a lower power tissue section through an adult sciatic nerve), Myelin Protein Zero (brown staining) can be seen in all of the myelinating Schwann cells.](http://images.novusbio.com/fullsize/Myelin-Protein-Zero-Antibody-Immunohistochemistry-NB100-1607-img0002.jpg)