| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71706SS | |||
| Azide and BSA Free | NBP2-80560 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-157SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89284-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-117SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77106-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-110SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72550 | |||
![Western Blot: ABCG8 Antibody (1B10A5) [NBP1-71706] - Analysis of ABCG8 expression in ABCG8 lysate using NBP1-71706.](http://images.novusbio.com/fullsize/ABCG8-Antibody-1B10A5-Western-Blot-NBP1-71706-img0006.jpg "Western Blot: ABCG8 Antibody (1B10A5) [NBP1-71706] - Analysis of ABCG8 expression in ABCG8 lysate using NBP1-71706.")
![Immunocytochemistry/Immunofluorescence: ABCG8 Antibody (1B10A5) [NBP1-71706] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were incubated with anti-ABCG8 Antibody (1B10A5) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCG8-Antibody-1B10A5-Immunocytochemistry-Immunofluorescence-NBP1-71706-img0007.jpg)
![Immunohistochemistry: ABCG8 Antibody (1B10A5) [NBP1-71706] - Immunohistochemical analysis of ABCG8 in mouse intestine using NBP1-71706.](http://images.novusbio.com/fullsize/ABCG8-Antibody-1B10A5-Immunohistochemistry-NBP1-71706-img0005.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - LXRs are expressed in peripheral blood cells. LXR alpha/NR1H3 protein levels in protein extracts from PBMCs from these same donors were detected by Western blotting using rabbit anti-human LXR alpha/NR1H3 polyclonal antisera. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-6-59), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NB300-612-img0013.jpg "Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - LXRs are expressed in peripheral blood cells. LXR alpha/NR1H3 protein levels in protein extracts from PBMCs from these same donors were detected by Western blotting using rabbit anti-human LXR alpha/NR1H3 polyclonal antisera. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-6-59), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody [NB300-612] - Analysis of LXR alpha (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-612-img0006.jpg)
![Flow Cytometry: LXR alpha/NR1H3 Antibody [NB300-612] - analysis of LXR alpha was done on HeLa. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton X-100 for 20 minutes, and blocked with 2.5% BSA for 30 minutes at room temperature. Cells were labeled with LXR alpha Rabbit Polyclonal Antibody (PA1330, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Flow-Cytometry-NB300-612-img0012.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - analysis was performed on nuclear enriched extracts (30 ug lysate) of MCF7 (Lane 1), THP-1 (Lane 2), A549 (Lane 3), SH-SY5Y (Lane 4), HL-60 (Lane 5) and KARPAS 299 (Lane 6). The blot was probed with Rabbit Anti-LXR alpha Polyclonal Antibody (2ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.25 ug/ml, 1:4000 dilution). A 50 kDa band corresponding to LXR alpha was observed across the cell lines tested. Known quantity of protein samples was electrophoresed using 4-12 % Bis-Tris gel, Electrophoresis System and Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with Blot 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using ECL Chemiluminescent Substrate Reagent Kit.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NB300-612-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody [NB300-612] - Analysis of LXR alpha (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-612-img0005.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody [NB400-157] - WB detection of LXR in human liver.](http://images.novusbio.com/fullsize/LXR-Antibody-Western-Blot-NB400-157-img0001.jpg "Western Blot: LXR alpha/NR1H3 Antibody [NB400-157] - WB detection of LXR in human liver.")
![Simple Western: LXR alpha/NR1H3 Antibody [NB400-157] - Simple Western lane view shows a ~49 kDa for LXR in human skeletal muscle tissue lysate under reducing conditions. Image from verified customer review.](http://images.novusbio.com/fullsize/LXR-Antibody-Simple-Western-NB400-157-img0002.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0009.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar pattern to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89284] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89284-img0007.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum shows moderate positivity in mitochondria in Purkinje cells.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0014.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89284] - ACAT1 porcine cerebral cortex. Image from verified customer review.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0008.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0011.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0012.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0013.jpg)
![Western Blot: ABCG8 Antibody [NB400-117] - Total protein from Mouse small intestine was separated on a 7.5% gel by SDS-PAGE and transferred to PVDF membrane. The membrane was then probed with anti-ABCG8 antibody at 2 ug/ml and detected with an anti-rabbit HRP conjugated secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCG8-Antibody-Western-Blot-NB400-117-img0003.jpg "Western Blot: ABCG8 Antibody [NB400-117] - Total protein from Mouse small intestine was separated on a 7.5% gel by SDS-PAGE and transferred to PVDF membrane. The membrane was then probed with anti-ABCG8 antibody at 2 ug/ml and detected with an anti-rabbit HRP conjugated secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ABCG8 Antibody [NB400-117] - ABCG8 antibody was tested in HEK293 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ABCG8-Antibody-Immunocytochemistry-Immunofluorescence-NB400-117-img0002.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Effects of FP on the expression of cholesterol metabolism-related genes and ABCA1 promoter activity in Caco-2 cells. After Caco-2 cells were treated with vehicle control or 1 mM FP for 24 h, total RNA and cell lysates were collected. ABCA1, NPC1L1, LXRalpha, and LXRbeta protein levels were analyzed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-56031-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NBP1-77106-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Effects of FP on the expression of cholesterol metabolism-related genes and ABCA1 promoter activity in Caco-2 cells. After Caco-2 cells were treated with vehicle control or 1 mM FP for 24 h, total RNA and cell lysates were collected. ABCA1, NPC1L1, LXRalpha, and LXRbeta protein levels were analyzed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-56031-8) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Rat liver tissue with LXR-A antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunohistochemistry-Paraffin-NBP1-77106-img0002.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Rat liver tissue lysate at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NBP1-77106-img0004.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Rat liver tissue with LXR-A antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunofluorescence-NBP1-77106-img0006.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunohistochemistry of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419541563.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunofluorescence of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419313242.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunohistochemistry of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419553048.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunofluorescence of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419432221.jpg)
![Western Blot: ABCG8 Antibody [NB400-110] - Analysis of ABCG8, using NB400-110. Samples: mouse liver microsomes from G5/G8 knockout mice, wildtype human G5/G8, and transgenic human G5/G8.](http://images.novusbio.com/fullsize/ABCG8-Antibody-Western-Blot-NB400-110-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCG8 Antibody [NB400-110] - Analysis of ABCG8, using NB400-110. Samples: mouse liver microsomes from G5/G8 knockout mice, wildtype human G5/G8, and transgenic human G5/G8.")
![Western Blot: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Western-Blot-NBP2-46220-img0017.jpg "Western Blot: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.")
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0016.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0001.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0002.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0003.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0004.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0005.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0006.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0007.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0008.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0009.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0010.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0011.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0012.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0013.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0014.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0015.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0009.jpg "Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Staining LXR alpha/NR1H3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunocytochemistry-Immunofluorescence-NBP2-66938-img0002.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded mouse testes tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0005.jpg)
![Flow Cytometry: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of HepG2 cells with LXR alpha/NR1H3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Flow-Cytometry-NBP2-66938-img0001.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha/NR1H3 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0006.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0007.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on mouse colon tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0008.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human placenta tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0003.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human spleen tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0004.jpg)
![Western Blot: LXR beta/NR1H2 Antibody [NB100-74457] - 25 ug of rat liver (Lane 1), mouse liver (Lane 2) and Hela (Lane 3) cell lysates.](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-Western-Blot-NB100-74457-img0004.jpg "Western Blot: LXR beta/NR1H2 Antibody [NB100-74457] - 25 ug of rat liver (Lane 1), mouse liver (Lane 2) and Hela (Lane 3) cell lysates.")
![Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody [NB100-74457] - Analysis of LXR beta (green) showing positive staining in the nuclear envelope of C2C12 cells (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74457-img0003.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Western blot analysis of extracts of various cell lines, using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Western-Blot-NBP3-16304-img0007.jpg "Western Blot: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Western blot analysis of extracts of various cell lines, using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunofluorescence analysis of NIH-3T3 cells using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Immunocytochemistry-Immunofluorescence-NBP3-16304-img0006.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunohistochemistry of paraffin-embedded mouse testis using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Immunohistochemistry-Paraffin-NBP3-16304-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunofluorescence analysis of C6 cells using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Immunocytochemistry-Immunofluorescence-NBP3-16304-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunofluorescence analysis of Hep G2 cells using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Immunocytochemistry-Immunofluorescence-NBP3-16304-img0005.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunohistochemistry of paraffin-embedded rat ovary using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Immunohistochemistry-Paraffin-NBP3-16304-img0001.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunohistochemistry of paraffin-embedded human colon using LXR alpha/NR1H3 Rabbit mAb (NBP3-16304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-7Z4H10-Immunohistochemistry-Paraffin-NBP3-16304-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunofluorescence analysis of C6 cells using LXR alpha/NR1H3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16304_rabbit-lxr-alpha-nr1h3-mab-7z4h10-5220251215493.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Confocal imaging of C2C12 cells using LXR alpha/NR1H3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16304_rabbit-lxr-alpha-nr1h3-mab-7z4h10-522025121547.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [NBP3-16304] - Immunofluorescence analysis of NIH-3T3 cells using LXR alpha/NR1H3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16304_rabbit-lxr-alpha-nr1h3-mab-7z4h10-52202511383726.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [LXR alpha/NR1H3] - Immunofluorescence analysis of NIH-3T3 cells using LXR alpha/NR1H3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16304_rabbit-lxr-alpha-nr1h3-mab-7z4h10-52202517304916.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody (7Z4H10) [LXR alpha/NR1H3] - Immunofluorescence analysis of C6 cells using LXR alpha/NR1H3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16304_rabbit-lxr-alpha-nr1h3-mab-7z4h10-5220251734586.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Western-Blot-NBP2-72550-img0001.jpg "Western Blot: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.")
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0016.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0002.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0003.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0004.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0005.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0006.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0007.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0008.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0009.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0010.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0011.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0012.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0013.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0014.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0015.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72550-img0017.jpg)