| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70871 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92879-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92734-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52471-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24754SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06277SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-166 | |||
| BSA Free | NB400-166SS | |||
| BSA Free | NB400-163SS | |||
| Azide and BSA Free | NBP2-80557 | |||
![Western Blot: ERAB Antibody (10B4) [NBP2-02118] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERAB monoclonal antibody.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Western-Blot-NBP2-02118-img0009.jpg "Western Blot: ERAB Antibody (10B4) [NBP2-02118] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERAB monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: ERAB Antibody (10B4) [NBP2-02118] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ERAB.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Immunocytochemistry-Immunofluorescence-NBP2-02118-img0008.jpg)
![Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded pancreas tissue using anti-ERABmouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Immunohistochemistry-Paraffin-NBP2-02118-img0007.jpg)
![Flow Cytometry: ERAB Antibody (10B4) [NBP2-02118] - Analysis of Hela cells, using anti-ERAB antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Flow-Cytometry-NBP2-02118-img0001.jpg)
![Western Blot: ERAB Antibody (10B4) [NBP2-02118] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERAB.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Western-Blot-NBP2-02118-img0002.jpg)
![Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERAB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Immunohistochemistry-Paraffin-NBP2-02118-img0003.jpg)
![Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-ERAB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Immunohistochemistry-Paraffin-NBP2-02118-img0004.jpg)
![Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded Kidney tissue using anti-ERAB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Immunohistochemistry-Paraffin-NBP2-02118-img0005.jpg)
![Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded liver tissue using anti-ERABmouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Immunohistochemistry-Paraffin-NBP2-02118-img0006.jpg)
![Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Western blot analysis of Mouse testes lysate (A) + peptide (B) with antibody at 1 ug/mL and Rat testes lysate (C) + peptide (D) with antibody at 0.3 ug/mL, 35ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Western-Blot-NBP1-00154-img0006.jpg "Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Western blot analysis of Mouse testes lysate (A) + peptide (B) with antibody at 1 ug/mL and Rat testes lysate (C) + peptide (D) with antibody at 0.3 ug/mL, 35ug protein in RIPA buffer. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00154-img0007.jpg)
![Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunohistochemistry-Paraffin-NBP1-00154-img0005.jpg)
![Flow Cytometry: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Flow-Cytometry-NBP1-00154-img0008.jpg)
![Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunohistochemistry-Paraffin-NBP1-00154-img0004.jpg)
![Western Blot: CYP11A1 Antibody [NBP2-92879] - Analysis of extracts of HeLa cells, using CYP11A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP2-92879-img0010.jpg "Western Blot: CYP11A1 Antibody [NBP2-92879] - Analysis of extracts of HeLa cells, using CYP11A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: CYP11A1 Antibody [NBP2-92879] - Analysis of extracts of various cell lines, using CYP11A1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP2-92879-img0008.jpg)
![Western Blot: CYP11A1 Antibody [NBP2-92879] - Analysis of extracts of various cell lines, using CYP11A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP2-92879-img0009.jpg)
![Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP2-92879] - Human colon using CYP11A1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Immunohistochemistry-Paraffin-NBP2-92879-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: CYP11A1 Antibody - Azide and BSA Free [NBP2-92879] - Immunofluorescence analysis of paraffin-embedded human placenta using CYP11A1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92879_rabbit-polyclonal-cyp11a1-antibody-42202514251825.jpg)
![Western Blot: Apolipoprotein CIII Antibody [NBP2-92734] - Analysis of extracts of various cell lines, using Apolipoprotein C3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Apolipoprotein-CIII-Antibody-Western-Blot-NBP2-92734-img0004.jpg "Western Blot: Apolipoprotein CIII Antibody [NBP2-92734] - Analysis of extracts of various cell lines, using Apolipoprotein C3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody [NBP2-92734] - Rat spleen using Apolipoprotein C3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Apolipoprotein-CIII-Antibody-Immunohistochemistry-Paraffin-NBP2-92734-img0003.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody [NBP2-92734] - Human liver using Apolipoprotein C3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Apolipoprotein-CIII-Antibody-Immunohistochemistry-Paraffin-NBP2-92734-img0001.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody [NBP2-92734] - Mouse spleen using Apolipoprotein C3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Apolipoprotein-CIII-Antibody-Immunohistochemistry-Paraffin-NBP2-92734-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein CIII Antibody [NBP2-92734] - Analysis of HepG2 cells using Apolipoprotein C3 Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Apolipoprotein-CIII-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92734-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Apolipoprotein CIII Antibody - Azide and BSA Free [NBP2-92734] - Immunofluorescence analysis of paraffin-embedded rat liver using Apolipoprotein CIII Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92734_rabbit-polyclonal-apolipoprotein-ciii-antibody-42202514251895.jpg)
![Immunocytochemistry/ Immunofluorescence: Apolipoprotein CIII Antibody - Azide and BSA Free [NBP2-92734] - Immunofluorescence analysis of paraffin-embedded mouse liver using Apolipoprotein CIII Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92734_rabbit-polyclonal-apolipoprotein-ciii-antibody-42202514251875.jpg)
![Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-Western-Blot-NBP2-52471-img0003.jpg "Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis of Hela cells using GAD2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-Flow-Cytometry-NBP2-52471-img0001.jpg)
![Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against human GAD2 (AA: 1-100) recombinant protein. (Expected MW is 36.3 kDa)](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-Western-Blot-NBP2-52471-img0002.jpg)
![ELISA: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-5D9G12-ELISA-NBP2-52471-img0004.jpg)
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Western-Blot-NBP2-22171-img0004.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Immunocytochemistry-Immunofluorescence-NBP2-22171-img0002.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Immunohistochemistry-Paraffin-NBP2-22171-img0003.jpg)
![Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Flow-Cytometry-NBP2-22171-img0001.jpg)
![Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Western-Blot-NBP2-67129-img0011.jpg "Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0009.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse heart tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0008.jpg)
![Flow Cytometry: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Flow-Cytometry-NBP2-67129-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0003.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0004.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0005.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0006.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0007.jpg)
![Western Blot: TIGAR/C12orf5 Antibody [NBP2-24754] - Analysis of TIGAR in human liver in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver and 4) rat liver lysate using TIGAR antibody at 1, 4 and 2 ug/ml, respectively.](http://images.novusbio.com/fullsize/TIGAR-C12orf5-Antibody-Western-Blot-NBP2-24754-img0004.jpg "Western Blot: TIGAR/C12orf5 Antibody [NBP2-24754] - Analysis of TIGAR in human liver in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver and 4) rat liver lysate using TIGAR antibody at 1, 4 and 2 ug/ml, respectively.")
![Immunohistochemistry-Paraffin: TIGAR/C12orf5 Antibody [NBP2-24754] - Analysis of human brain, cerebellum, using TIGAR antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/TIGAR-C12orf5-Antibody-Immunohistochemistry-Paraffin-NBP2-24754-img0003.jpg)
![Western Blot: ESCO2 Antibody [NB100-87021] - Detection of Human ESCO2 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ESCO2 antibody NB100-87021 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ESCO2-Antibody-Western-Blot-NB100-87021-img0007.jpg "Western Blot: ESCO2 Antibody [NB100-87021] - Detection of Human ESCO2 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ESCO2 antibody NB100-87021 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ESCO2 Antibody [NB100-87021] - Detection of human ESCO2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ESCO2 antibody NB100-87021 (lot 2) used for IP at 6 ug per reaction. ESCO2 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-ESCO2 antibody from Company B. For blotting immunoprecipitated ESCO2, NB100-87021 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ESCO2-Antibody-Immunoprecipitation-NB100-87021-img0008.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-06277] - Detection of Bip/Grp78 in rat liver.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06277-img0005.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP1-06277] - Detection of Bip/Grp78 in rat liver.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06277] - BIP/Grp78 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06277-img0006.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06277] - Lane view shows a specific band for GRP78 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06277-img0008.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06277] - Lane view shows a specific band detected for GRP78 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06277-img0007.jpg)
![Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Total protein from mouse platelets was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA7 in 5% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Western-Blot-NB400-163-img0010.jpg "Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Total protein from mouse platelets was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA7 in 5% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence.")
![Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Analysis using Azide/BSA FREE version of NB400-163. Detection of ABCA7 using NB 400-166 at various dilutions in ABCA7 transfected HeLa cell lysates.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Western-Blot-NB400-163-img0008.jpg)
![Flow Cytometry: ABCA7 Antibody (7A1-144) [NB400-163] - Analysis using Azide/BSA FREE version of NB400-163. Analysis of 6C (red) (negative control HeLa cells) and A7(blue) (ABCA7 expressing HeLa cells) using NB 400-166 at a dilution of 1:3,000.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Flow-Cytometry-NB400-163-img0007.jpg)
![Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Detection of ABCA7 using NB 400-163 at various dilutions in ABCA7 transfected HeLa lysates.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Western-Blot-NB400-163-img0006.jpg)
![Flow Cytometry: ABCA7 Antibody (7A1-144) [NB400-163] - FACS analysis of 6C (red) (negative control HeLa cells) and A7 (blue) (ABCA7 expressing HeLa cells) using NB 400-163 at a 1:2,000 dilution.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Flow-Cytometry-NB400-163-img0005.jpg)
![Western Blot: GRP78 Antibody [NBP2-16749] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/GRP78-Antibody-Western-Blot-NBP2-16749-img0002.jpg "Western Blot: GRP78 Antibody [NBP2-16749] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0005.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse intestine. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0015.jpg)
![Grp78 antibody detects Grp78 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Grp78 stained by Grp78 antibody (NBP2-16749) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16749_rabbit-polyclonal-grp78-hspa5-antibody-immunocytochemistry-immunofluorescence-261120251823305.jpg)
