| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70871 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37300-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89152-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87871-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 488 | NB110-37253G | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-00589SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44465 | |||
![Western Blot: ERAB Antibody (10B4) [NBP2-02118] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERAB monoclonal antibody.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Western-Blot-NBP2-02118-img0009.jpg "Western Blot: ERAB Antibody (10B4) [NBP2-02118] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERAB monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: ERAB Antibody (10B4) [NBP2-02118] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ERAB.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Immunocytochemistry-Immunofluorescence-NBP2-02118-img0008.jpg)
![Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded pancreas tissue using anti-ERABmouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Immunohistochemistry-Paraffin-NBP2-02118-img0007.jpg)
![Flow Cytometry: ERAB Antibody (10B4) [NBP2-02118] - Analysis of Hela cells, using anti-ERAB antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Flow-Cytometry-NBP2-02118-img0001.jpg)
![Western Blot: ERAB Antibody (10B4) [NBP2-02118] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERAB.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Western-Blot-NBP2-02118-img0002.jpg)
![Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERAB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Immunohistochemistry-Paraffin-NBP2-02118-img0003.jpg)
![Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-ERAB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Immunohistochemistry-Paraffin-NBP2-02118-img0004.jpg)
![Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded Kidney tissue using anti-ERAB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Immunohistochemistry-Paraffin-NBP2-02118-img0005.jpg)
![Immunohistochemistry-Paraffin: ERAB Antibody (10B4) [NBP2-02118] - Staining of paraffin-embedded liver tissue using anti-ERABmouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERAB-Antibody-10B4-Immunohistochemistry-Paraffin-NBP2-02118-img0006.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0004.jpg "Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.")
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Immunofluorescence analysis of HepG2 cells using PLIN2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunocytochemistry-Immunofluorescence-NBP2-37300-img0005.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of bladder cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0008.jpg)
![Flow Cytometry: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of HepG2 cells using PLIN2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Flow-Cytometry-NBP2-37300-img0006.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against human PLIN2 (AA: 286-437) recombinant protein. (Expected MW is 42.6 kDa)](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0002.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against HEK293 (1) and PLIN2 (AA: 286-437)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0003.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of rectum cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0007.jpg)
![ELISA: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-ELISA-NBP2-37300-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - All lanes: Western blot analysis of GBA with anti-GBA antibody [JM10-76 at 1:1,000 dilution.Lane 1: Wild-type HEK293T whole cell lysate (20 ug).Lane 2: GBA knockout HEK293T whole cell lysate (20 ug). NBP2-66871was shown to specifically react with GBA in wild-type HEK293T cells. No band was observed when GBA knockout sample was tested. Wild-type and GBA knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH , 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Western-Blot-NBP2-66871-img0011.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded mouse pancreas tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0015.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Western blot analysis of Glucosylceramidase/GBA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: A549 cell lysate Lane 3: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Western-Blot-NBP2-66871-img0009.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-GBA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0001.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human liver tissue using anti-GBA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0003.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human pancreas tissue using anti-GBA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human kidney tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded rat liver tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0013.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded rat kidney tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0014.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Analysis in human cell lines A-549 and A-431 using anti-ALDH1A1 antibody. Corresponding ALDH1A1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP1-89152-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Analysis in human cell lines A-549 and A-431 using anti-ALDH1A1 antibody. Corresponding ALDH1A1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human cell line A549 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89152-img0006.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human tonsil shows very weak positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89152-img0015.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89152-img0012.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89152-img0013.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89152-img0014.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0013.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human cervix shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0010.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0011.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Analysis of methanol-fixed A431, using LDHA antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19320-img0015.jpg "Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Analysis of methanol-fixed A431, using LDHA antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Mouse liver. LDHA stained by LDHA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-Immunohistochemistry-Paraffin-NBP2-19320-img0017.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Human lung papillory adenocarcinoma, using LDHA antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-19320-img0002.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 L](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70169-img0004.jpg "Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 L")
![Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining in COS7 cells](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70169-img0005.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Human breast tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0011.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70169-img0001.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Analysis of extracts (35ug) from 9 different cell lines by using anti-15 Lipoxygenase 1 monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70169-img0002.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0006.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0007.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0008.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0009.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0010.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0012.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of Cytochrome P450 17A1 on rat testis tissue lysates with Rabbit anti-Cytochrome P450 17A1 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Western-Blot-NBP2-75461-img0008.jpg "Western Blot: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of Cytochrome P450 17A1 on rat testis tissue lysates with Rabbit anti-Cytochrome P450 17A1 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Staining Cytochrome P450 17A1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunocytochemistry-Immunofluorescence-NBP2-75461-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of paraffin-embedded mouse kidney tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunohistochemistry-Paraffin-NBP2-75461-img0007.jpg)
![Flow Cytometry: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of SH-SY-5Y cells with Cytochrome P450 17A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Flow-Cytometry-NBP2-75461-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Staining Cytochrome P450 17A1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunocytochemistry-Immunofluorescence-NBP2-75461-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Staining Cytochrome P450 17A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunocytochemistry-Immunofluorescence-NBP2-75461-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of paraffin-embedded human breast tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunohistochemistry-Paraffin-NBP2-75461-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunohistochemistry-Paraffin-NBP2-75461-img0006.jpg)
![Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).](http://images.novusbio.com/fullsize/HSL-Antibody-Western-Blot-NB110-37253-img0002.jpg "Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).")
![Western Blot: NHE3/SLC9A3 Antibody [NBP3-00589] - Total protein from human Hek293 and HeLa cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NHE3 (NBP3-00589) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Western-Blot-NBP3-00589-img0003.jpg "Western Blot: NHE3/SLC9A3 Antibody [NBP3-00589] - Total protein from human Hek293 and HeLa cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NHE3 (NBP3-00589) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: NHE3/SLC9A3 Antibody [NBP3-00589] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-NHE3/SLC9A3 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-00589-img0002.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP3-00589] - Analysis of a FFPE tissue section of human small intestine using 1:200 dilution of NHE3 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This NHE3 antibody generated nuclear staining.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP3-00589-img0001.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining in human liver and pancreas tissues. Corresponding ABCD3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0025.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining in human liver and pancreas tissues. Corresponding ABCD3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PMP70 Antibody (CL2524) [NBP2-36770] - Staining in U251 cell line showing specific staining of peroxisomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunocytochemistry-Immunofluorescence-NBP2-36770-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (CL2524) [NBP2-36770] - Staining in HeLa cell line showing specific staining of peroxisomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunocytochemistry-Immunofluorescence-NBP2-36770-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human cell line A431 showing specific staining of peroxisomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunocytochemistry-Immunofluorescence-NBP2-36770-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (CL2524) [NBP2-36770] - Staining in MCF7 cell line showing specific staining of peroxisomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunocytochemistry-Immunofluorescence-NBP2-36770-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (CL2524) [NBP2-36770] - Staining in U2OS cell line showing specific staining of peroxisomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunocytochemistry-Immunofluorescence-NBP2-36770-img0010.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human rectum shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0015.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0019.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0020.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0021.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human fallopian tube shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0023.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human pancreas shows weak granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0024.jpg)
![Immunohistochemistry-Paraffin: Sorbitol Dehydrogenase Antibody [NBP1-87416] - Analysis in human prostate and skeletal muscle tissues using NBP1-87416 antibody. Corresponding SORD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Sorbitol-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-87416-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Sorbitol Dehydrogenase Antibody [NBP1-87416] - Analysis in human prostate and skeletal muscle tissues using NBP1-87416 antibody. Corresponding SORD RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Sorbitol Dehydrogenase Antibody [NBP1-87416] - Staining of human kidney, liver, prostate and skeletal muscle using Anti-SORD antibody NBP1-87416 (A) shows similar protein distribution across tissues to independent antibody NBP1-87415 (B).](http://images.novusbio.com/fullsize/Sorbitol-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-87416-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Sorbitol Dehydrogenase Antibody [NBP1-87416] - Analysis using Anti-SORD antibody NBP1-87416 (A) shows similar pattern to independent antibody NBP1-87415 (B).](http://images.novusbio.com/fullsize/Sorbitol-Dehydrogenase-Antibody-Western-Blot-NBP1-87416-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Sorbitol Dehydrogenase Antibody [NBP1-87416] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Sorbitol-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87416-img0005.jpg)
![Immunohistochemistry-Paraffin: Sorbitol Dehydrogenase Antibody [NBP1-87416] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Sorbitol-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-87416-img0020.jpg)
![Immunohistochemistry-Paraffin: Sorbitol Dehydrogenase Antibody [NBP1-87416] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Sorbitol-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-87416-img0018.jpg)
![Immunohistochemistry-Paraffin: Sorbitol Dehydrogenase Antibody [NBP1-87416] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Sorbitol-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-87416-img0019.jpg)
![Immunohistochemistry-Paraffin: Sorbitol Dehydrogenase Antibody [NBP1-87416] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Sorbitol-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-87416-img0021.jpg)