| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB3664-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-166 | |||
| BSA Free | NB400-166SS | |||
| BSA Free | NB400-163SS | |||
| Azide and BSA Free | NBP2-80557 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58749SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 488 | NB110-37253G | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27345SS | |||
| Azide and BSA Free | NBP2-80793 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-00589SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91705-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - Analysis of HepG2 cell lysate with 0.1 ug/ml of hApoA1 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hApoA1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-2083D-Western-Blot-MAB3664-img0002.jpg "Western Blot: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - Analysis of HepG2 cell lysate with 0.1 ug/ml of hApoA1 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hApoA1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Immunocytochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - ApoA1 was detected in immersion fixed HepG2 cell using ApoA1 Monoclonal Antibody at 1 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-2083D-Immunocytochemistry-MAB3664-img0001.jpg)
![Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - ApoA1 was detected in immersion fixed paraffin-embedded sections of human lung cancer using ApoA1 Monoclonal Antibody at 1 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-2083D-Immunohistochemistry-MAB3664-img0003.jpg)
![Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - An intracellular stain was performed on HepG2 cells with Apolipoprotein A-I/ApoA1 Antibody (2083D) MAB3664AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-2083D-Flow-Cytometry-MAB3664-img0006.jpg)
![Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB3664, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-2083D-Flow-Cytometry-MAB3664-img0005.jpg)
![Western Blot: Importin-13 Antibody [NBP1-31508] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 7.5 % SDS-PAGE Importin 13 antibody [C3], C-term dilution: 1:1000](http://images.novusbio.com/fullsize/Importin-13-Antibody-Western-Blot-NBP1-31508-img0012.jpg "Western Blot: Importin-13 Antibody [NBP1-31508] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 7.5 % SDS-PAGE Importin 13 antibody [C3], C-term dilution: 1:1000")
![Immunohistochemistry-Paraffin: Importin-13 Antibody [NBP1-31508] - CL1-5 xenograft, using Importin 13 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Importin-13-Antibody-Immunohistochemistry-Paraffin-NBP1-31508-img0010.jpg)
![Western Blot: Importin-13 Antibody [NBP1-31508] - IPO13 was immunoprecipitated from 1mg of Hela whole cell lysate using 10 ug of anti-IPO13 antibody. The precipitated IPO13 was detected by antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Importin-13-Antibody-Western-Blot-NBP1-31508-img0007.jpg)
![Western Blot: Importin-13 Antibody [NBP1-31508] - Analysis of IPO13 expression in transfected 293T cell line by IPO13 polyclonal antibody. A: Non-transfected lysate. B: IPO13 transfected lysate. 7.5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Importin-13-Antibody-Western-Blot-NBP1-31508-img0008.jpg)
![Western Blot: CBS Antibody (519) [NBP2-43604] - Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CBS-Antibody-519-Western-Blot-NBP2-43604-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CBS Antibody (519) [NBP2-43604] - Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: CBS Antibody (519) [NBP2-43604] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (519) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CBS-Antibody-519-Western-Blot-NBP2-43604-img0008.jpg)
![Immunohistochemistry-Paraffin: CBS Antibody (519) [NBP2-43604] - Paraffin-embedded mouse kidney. CBS antibody [519] diluted at 1:250.](http://images.novusbio.com/fullsize/CBS-Antibody-519-Immunohistochemistry-Paraffin-NBP2-43604-img0006.jpg)
![Western Blot: CBS Antibody (519) [NBP2-43604] - Analysis of A. 30 ug Raji whole cell lysate/extract B. 30 ug K562 whole cell lysate/extract 7.5 % SDS-PAGE CBS antibody [519] dilution: 1:1000](http://images.novusbio.com/fullsize/CBS-Antibody-519-Western-Blot-NBP2-43604-img0001.jpg)
![Western Blot: CBS Antibody (519) [NBP2-43604] - Analysis of A. 50 ug mouse liver lysate/extract 7.5 % SDS-PAGE CBS antibody [519] dilution: 1:2000.](http://images.novusbio.com/fullsize/CBS-Antibody-519-Western-Blot-NBP2-43604-img0002.jpg)
![Western Blot: CBS Antibody (519) [NBP2-43604] - Analysis of A. 50 ug rat liver lysate/extract 7.5 % SDS-PAGE CBS antibody [519] dilution: 1:1000](http://images.novusbio.com/fullsize/CBS-Antibody-519-Western-Blot-NBP2-43604-img0003.jpg)
![Immunohistochemistry-Paraffin: CBS Antibody (519) [NBP2-43604] - Paraffin-embedded rat liver. CBS antibody [519] diluted at 1:250.](http://images.novusbio.com/fullsize/CBS-Antibody-519-Immunohistochemistry-Paraffin-NBP2-43604-img0005.jpg)
![Various tissue extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (NBP2-43604) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43604_mouse-monoclonal-cbs-antibody-519-western-blot-2611202518425420.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (NBP2-43604) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43604_mouse-monoclonal-cbs-antibody-519-western-blot-261120251832450.jpg)
![Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Total protein from mouse platelets was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA7 in 5% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Western-Blot-NB400-163-img0010.jpg "Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Total protein from mouse platelets was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA7 in 5% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence.")
![Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Analysis using Azide/BSA FREE version of NB400-163. Detection of ABCA7 using NB 400-166 at various dilutions in ABCA7 transfected HeLa cell lysates.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Western-Blot-NB400-163-img0008.jpg)
![Flow Cytometry: ABCA7 Antibody (7A1-144) [NB400-163] - Analysis using Azide/BSA FREE version of NB400-163. Analysis of 6C (red) (negative control HeLa cells) and A7(blue) (ABCA7 expressing HeLa cells) using NB 400-166 at a dilution of 1:3,000.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Flow-Cytometry-NB400-163-img0007.jpg)
![Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Detection of ABCA7 using NB 400-163 at various dilutions in ABCA7 transfected HeLa lysates.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Western-Blot-NB400-163-img0006.jpg)
![Flow Cytometry: ABCA7 Antibody (7A1-144) [NB400-163] - FACS analysis of 6C (red) (negative control HeLa cells) and A7 (blue) (ABCA7 expressing HeLa cells) using NB 400-163 at a 1:2,000 dilution.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Flow-Cytometry-NB400-163-img0005.jpg)
![Western Blot: 5-Lipoxygenase Antibody [NB110-58749] - Western blotting data for TLR4, COX-2, IL-1beta, iNOS, 5-Lipoxygenase. Data are representative of three independent experiment. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2020.00142/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Western-Blot-NB110-58749-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: 5-Lipoxygenase Antibody [NB110-58749] - Western blotting data for TLR4, COX-2, IL-1beta, iNOS, 5-Lipoxygenase. Data are representative of three independent experiment. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2020.00142/full), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: 5-Lipoxygenase Antibody [NB110-58749] - Detection of LOX5 in human peripheral leukocytes using NB110-58749 at 0.5(Lane 1) and 2.0 ug/ml (Lane 2).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Western-Blot-NB110-58749-img0002.jpg)
![Simple Western: 5-Lipoxygenase Antibody [NB110-58749] - Simple Western lane view shows a specific band for 5-Lipoxygenase in 0.5 mg/ml of Human Lung lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Simple-Western-NB110-58749-img0003.jpg)
![Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).](http://images.novusbio.com/fullsize/HSL-Antibody-Western-Blot-NB110-37253-img0002.jpg "Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).")
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0008.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Intracellular staining of Interleukin-1 beta positive T cells (upper panel) & monocytes (lower panel) from stimulated PBMC (with a cocktail of TLR ligands and PMA/IM for 6h) using Interleukin-1 beta antibody at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Flow-Cytometry-NBP2-27345-img0006.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of full length human IL-1 beta recombinant protein (10ng/well) using IL1 beta Antibody (NBP2-27345) at 1:1000 (left lane) and 1:100 (right lane).](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0009.jpg)
![Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in human kidney tissue using Interleukin-1 beta antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Immunohistochemistry-Paraffin-NBP2-27345-img0007.jpg)
![Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Western-Blot-H00004830-M02-img0014.jpg "Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Immunocytochemistry-Immunofluorescence-H00004830-M02-img0007.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Immunohistochemistry-Paraffin-H00004830-M02-img0009.jpg)
![Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - NME1 monoclonal antibody (M02), clone 1D7. Analysis of NME1 expression in HeLa.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Western-Blot-H00004830-M02-img0012.jpg)
![Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - NME1 monoclonal antibody (M02), clone 1D7. Analysis of NME1 expression in different cell lines.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Western-Blot-H00004830-M02-img0013.jpg)
![Immunoprecipitation: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 transfected lysate using anti-NME1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NME1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Immunoprecipitation-H00004830-M02-img0010.jpg)
![ELISA: NM23-H1 Antibody (1D7) [H00004830-M02] - Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-ELISA-H00004830-M02-img0011.jpg)
![Western Blot: NHE3/SLC9A3 Antibody [NBP3-00589] - Total protein from human Hek293 and HeLa cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NHE3 (NBP3-00589) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Western-Blot-NBP3-00589-img0003.jpg "Western Blot: NHE3/SLC9A3 Antibody [NBP3-00589] - Total protein from human Hek293 and HeLa cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NHE3 (NBP3-00589) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: NHE3/SLC9A3 Antibody [NBP3-00589] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-NHE3/SLC9A3 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-00589-img0002.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP3-00589] - Analysis of a FFPE tissue section of human small intestine using 1:200 dilution of NHE3 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This NHE3 antibody generated nuclear staining.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP3-00589-img0001.jpg)
![Western Blot: ACSL3 Antibody [NBP1-89268] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-ACSL3 antibody. Corresponding ACSL3 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Western-Blot-NBP1-89268-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: ACSL3 Antibody [NBP1-89268] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-ACSL3 antibody. Corresponding ACSL3 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ACSL3 Antibody [NBP1-89268] - Analysis in control (vector only transfected HEK293T lysate) and ACSL3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ACSL3-Antibody-Western-Blot-NBP1-89268-img0013.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP1-89268] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunohistochemistry-Paraffin-NBP1-89268-img0010.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP1-89268] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunohistochemistry-Paraffin-NBP1-89268-img0007.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP1-89268] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunohistochemistry-Paraffin-NBP1-89268-img0008.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP1-89268] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunohistochemistry-Paraffin-NBP1-89268-img0009.jpg)
![Western Blot: BRAP Antibody [NBP1-91705] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/BRAP-Antibody-Western-Blot-NBP1-91705-img0007.jpg "Western Blot: BRAP Antibody [NBP1-91705] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: BRAP Antibody [NBP1-91705] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.](http://images.novusbio.com/fullsize/BRAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91705-img0008.jpg)
![Immunohistochemistry-Paraffin: BRAP Antibody [NBP1-91705] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/BRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91705-img0011.jpg)
![Western Blot: BRAP Antibody [NBP1-91705] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/BRAP-Antibody-Western-Blot-NBP1-91705-img0006.jpg)
![Immunohistochemistry-Paraffin: BRAP Antibody [NBP1-91705] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/BRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91705-img0009.jpg)
![Immunohistochemistry-Paraffin: BRAP Antibody [NBP1-91705] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/BRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91705-img0010.jpg)
![Immunohistochemistry-Paraffin: BRAP Antibody [NBP1-91705] - Staining of human Fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/BRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91705-img0012.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-91705_rabbit-polyclonal-brap-antibody-3052025751303.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)