| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-57150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-55454SS | |||
| Azide and BSA Free | NBP2-80580 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06277SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23610SS | |||
| Azide and BSA Free | NBP2-80919 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| BSA Free | NBP2-76810 | |||
![Western Blot: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis on different cell lysates using anti-PI3-kinase p85 subunit alpha Mouse mAb. Positive control: Lane 1: U-937 Lane 2: Raji](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Western-Blot-NBP2-67058-img0006.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis on different cell lysates using anti-PI3-kinase p85 subunit alpha Mouse mAb. Positive control: Lane 1: U-937 Lane 2: Raji")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of MCF-7 cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunocytochemistry-Immunofluorescence-NBP2-67058-img0008.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of paraffin- embedded mouse skeletal muscle tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunohistochemistry-Paraffin-NBP2-67058-img0009.jpg)
![Flow Cytometry: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of MCF-7 cells with PI3-kinase p85 subunit alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondar](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Flow-Cytometry-NBP2-67058-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of Hela cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunocytochemistry-Immunofluorescence-NBP2-67058-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of SH-SY-5Y cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunocytochemistry-Immunofluorescence-NBP2-67058-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of PANC-1 cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunocytochemistry-Immunofluorescence-NBP2-67058-img0007.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of paraffin- embedded human breast carcinoma tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunohistochemistry-Paraffin-NBP2-67058-img0004.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of paraffin- embedded human colon cancer tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunohistochemistry-Paraffin-NBP2-67058-img0005.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of PI 3 Kinase p85 alpha on different lysates using anti-PI 3 Kinase p85 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Raji](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Western-Blot-NBP2-67488-img0010.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of PI 3 Kinase p85 alpha on different lysates using anti-PI 3 Kinase p85 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Raji")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0005.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded mouse kidney tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0009.jpg)
![Flow Cytometry: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of HepG2 cells with PI 3 Kinase p85 alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antib](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Flow-Cytometry-NBP2-67488-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0004.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded human kidney tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0006.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded human liver cancer tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0007.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded mouse heart tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0008.jpg)
![Western Blot: PINK1 Antibody [NB600-973] - Lane 1: The primary band with the observed molecular weight of 66 kDa was detected in mouse liver (30ug). Also, a truncated form of the protein at about 33 kDa was detected.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB600-973-img0001.jpg "Western Blot: PINK1 Antibody [NB600-973] - Lane 1: The primary band with the observed molecular weight of 66 kDa was detected in mouse liver (30ug). Also, a truncated form of the protein at about 33 kDa was detected.")
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NB600-973] - LRRK2 accumulates in globules in alphaS tg mice. Double immunofluorescence for alphaS with parkin, PINK1, DJ-1, LRRK2, or negative control (the immunopeptide-preabsorbed anti-LRRK2 antibody) in alphaS tg mice. Scale bar = 5 um for all panels. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-34) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-973-img0003.jpg)
![Immunohistochemistry-Paraffin: PINK1 Antibody [NB600-973] - Staining in human liver tissue using a 1:200 dilution.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunohistochemistry-Paraffin-NB600-973-img0002.jpg)
![Western Blot: LPIN1 Antibody [NB110-57150] - Detection of LPIN1 in 3T3L1 lysate.](http://images.novusbio.com/fullsize/LPIN1-Antibody-Western-Blot-NB110-57150-img0003.jpg "Western Blot: LPIN1 Antibody [NB110-57150] - Detection of LPIN1 in 3T3L1 lysate.")
![Immunohistochemistry: LPIN1 Antibody [NB110-57150] - Staining of LPIN1 in mouse pancreas.](http://images.novusbio.com/fullsize/LPIN1-Antibody-Immunohistochemistry-NB110-57150-img0004.jpg)
![Western Blot: PDX1 Antibody [NBP2-22150] - WB analysis of PDX1 in INS1 cell lysate.](http://images.novusbio.com/fullsize/PDX1-Antibody-Western-Blot-NBP2-22150-img0003.jpg "Western Blot: PDX1 Antibody [NBP2-22150] - WB analysis of PDX1 in INS1 cell lysate.")
![Immunocytochemistry/Immunofluorescence: PDX1 Antibody [NBP2-22150] - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22150-img0001.jpg)
![Immunohistochemistry: PDX1 Antibody [NBP2-22150] - IHC analysis of PDX1 in mouse pancreas.](http://images.novusbio.com/fullsize/PDX1-Antibody-Immunohistochemistry-NBP2-22150-img0002.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Analysis in human serum total protein (lane 2). Lane 1 is a MW standard.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB110-55454-img0009.jpg "Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Analysis in human serum total protein (lane 2). Lane 1 is a MW standard.")
![Immunohistochemistry-Paraffin: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using 5 ug/ml conc. of Apolipoprotein A5 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Apolipoprotein A5 in hepatocytes was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Immunohistochemistry-Paraffin-NB110-55454-img0011.jpg)
![Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Apolipoprotein A5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Flow-Cytometry-NB110-55454-img0007.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Analysis of ApoA5 in HepG2 lysates](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB110-55454-img0002.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Detection of Apo A5 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB110-55454-img0008.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-06277] - Detection of Bip/Grp78 in rat liver.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06277-img0005.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP1-06277] - Detection of Bip/Grp78 in rat liver.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06277] - BIP/Grp78 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06277-img0006.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06277] - Lane view shows a specific band for GRP78 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06277-img0008.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06277] - Lane view shows a specific band detected for GRP78 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06277-img0007.jpg)
![Western Blot: GRP78 Antibody [NBP2-16749] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/GRP78-Antibody-Western-Blot-NBP2-16749-img0002.jpg "Western Blot: GRP78 Antibody [NBP2-16749] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0005.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse intestine. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0015.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - GRP78/HSPA5 proteins were measured by immunoblotting in epithelioid (EPI BAP1+Â UPN7), biphasic (BIP BAP1-Â UPN16) and sarcomatous (BAP1+Â UPN22) MPM cells. Tubulin was used as a loading control. The figure is representative of 1 out of 3 experiments with similar results. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35197103/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-16749_rabbit-polyclonal-grp78-hspa5-antibody-25520231539516.jpg)
![Grp78 antibody detects Grp78 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Grp78 stained by Grp78 antibody (NBP2-16749) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16749_rabbit-polyclonal-grp78-hspa5-antibody-immunocytochemistry-immunofluorescence-261120251823305.jpg)
![Western Blot: PON1 Antibody (4G8D3) [NBP2-23610] - Western blot analysis of PON1 in (1) HEK293 and (2) PON1 hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/PON1-Antibody-4G8D3-Western-Blot-NBP2-23610-img0007.jpg "Western Blot: PON1 Antibody (4G8D3) [NBP2-23610] - Western blot analysis of PON1 in (1) HEK293 and (2) PON1 hIgGFc transfected HEK293 cell lysate.")
![Immunohistochemistry: PON1 Antibody (4G8D3) [NBP2-23610] - Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues with DAB staining.](http://images.novusbio.com/fullsize/PON1-Antibody-4G8D3-Immunohistochemistry-NBP2-23610-img0006.jpg)
![Flow Cytometry: PON1 Antibody (4G8D3) [NBP2-23610] - Flow cytometric analysis of Hela cells using PON1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PON1-Antibody-4G8D3-Flow-Cytometry-NBP2-23610-img0004.jpg)
![Western Blot: PON1 Antibody (4G8D3) [NBP2-23610] - Western blot analysis of PON1 in human PON1 recombinant protein. (Expected MW is 40.6 kDa).](http://images.novusbio.com/fullsize/PON1-Antibody-4G8D3-Western-Blot-NBP2-23610-img0002.jpg)
![Western Blot: PON1 Antibody (4G8D3) [NBP2-23610] - Western blot analysis of PON1 in human plasma cell lysate.](http://images.novusbio.com/fullsize/PON1-Antibody-4G8D3-Western-Blot-NBP2-23610-img0003.jpg)
![Immunohistochemistry: PON1 Antibody (4G8D3) [NBP2-23610] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues with DAB staining.](http://images.novusbio.com/fullsize/PON1-Antibody-4G8D3-Immunohistochemistry-NBP2-23610-img0005.jpg)
![Western Blot: Islet-1 Antibody (2E7) [H00003670-M05] - Analysis of ISL1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Islet-1-Antibody-2E7-Western-Blot-H00003670-M05-img0004.jpg "Western Blot: Islet-1 Antibody (2E7) [H00003670-M05] - Analysis of ISL1 expression in PC-12 (Cat # L012V1).")
![Immunocytochemistry/Immunofluorescence: Islet-1 Antibody (2E7) [H00003670-M05] - Analysis of monoclonal antibody to ISL1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Islet-1-Antibody-2E7-Immunocytochemistry-Immunofluorescence-H00003670-M05-img0003.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Western-Blot-NBP2-80713-img0001.jpg "Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80713-img0002.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80713-img0003.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit Anti- EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80713-img0004.jpg)