| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75478SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70207 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22166SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86893-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56368SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52983SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89284-25ul | |||
![Western Blot: PDZK1 Antibody [NB400-149] - WB analysis of PDZK1 in fetal human liver lysate.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Western-Blot-NB400-149-img0003.jpg "Western Blot: PDZK1 Antibody [NB400-149] - WB analysis of PDZK1 in fetal human liver lysate.")
![Immunocytochemistry/Immunofluorescence: PDZK1 Antibody [NB400-149] - PDZK1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-149-img0004.jpg)
![Immunohistochemistry: PDZK1 Antibody [NB400-149] - IHC analysis of PDZK1 in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunohistochemistry-NB400-149-img0005.jpg)
![Simple Western: PDZK1 Antibody [NB400-149] - Simple Western lane view shows a specific band for PDZK1 in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Simple-Western-NB400-149-img0006.jpg)
![Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-75478-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.")
![<b>Biological Strategies Validation. </b>Simple Western: ATF6 Antibody [NBP1-75478] - Simple Western lane view shows a specific band for ATF6 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/ATF6-Antibody-Simple-Western-NBP1-75478-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-ATF6 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75478-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody - BSA Free [NBP1-75478] - AICAR inhibits ER stress responses. Western analysis of cell lysates of nuclear extracts. AMPK-independent inhibition of human macrophage ER stress response by AICAR. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27562249/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-75478-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - Antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75478-img0002.jpg)
![Western Blot: BMAL1 Antibody (OTI1C11) [NBP2-02544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMAL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMAL1.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Western-Blot-NBP2-02544-img0013.jpg "Western Blot: BMAL1 Antibody (OTI1C11) [NBP2-02544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMAL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMAL1.")
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNTL .](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunocytochemistry-Immunofluorescence-NBP2-02544-img0014.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0012.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue N93ing anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0003.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded colon tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0006.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded lung tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0007.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded pancreas tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0008.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded prostate tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0009.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0010.jpg)
![Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded bladder tissue using anti-BMAL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BMAL1-Antibody-OTI1C11-Immunohistochemistry-Paraffin-NBP2-02544-img0011.jpg)
![Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP2-22166-img0006.jpg "Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - 293T cells transfected with IDH2-flag expression construct were fixed and stained with anti-IDH2 antibody followed by Alexa Fluor 488 anti-rabbit secondary antibody. DAPI staining shows nuclei. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22166-img0007.jpg)
![Immunohistochemistry: IDH2 Antibody [NBP2-22166] - Staining of IDH2 in mouse intestine.](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunohistochemistry-NBP2-22166-img0003.jpg)
![Western Blot: IDH2 Antibody [NBP2-22166] - Analysis of Caki-1 cell lysate (A), human skeletal muscle tissue (B), mouse skeletal muscle tissue (C), and rat skeletal muscle tissue (D) using IDH2 antibody at a concentration of 2 ug/mL.](http://images.novusbio.com/fullsize/IDH2-Antibody-Western-Blot-NBP2-22166-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - IDH2 antibody was tested in U2OS cells with DyLight (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/IDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22166-img0001.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0011.jpg "Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.")
![Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 2 ug/ml.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Immunohistochemistry-Paraffin-H00055743-M01-img0008.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Western Blot analysis of CHFR expression in A-431 ( Cat # L015V1 ).](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0002.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( Cat # H00055743-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (Cat # H00055743-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0009.jpg)
![Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12.Lane 1: CHFR transfected lysate (Predicted MW: 72.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Western-Blot-H00055743-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Immunohistochemistry-Paraffin-H00055743-M01-img0003.jpg)
![Sandwich ELISA: CHFR Antibody (1H3-A12) [H00055743-M01] - Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CHFR-Antibody-1H3-A12-Sandwich-ELISA-H00055743-M01-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: TRP2 Antibody [NBP1-86893] - Mouse melanoblasts were incubated for 1h at 37C with anti-Trp2 rabbit 1:200, followed by incubation with Donkey anti-rabbit AF488. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TRP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86893-img0016.jpg "Immunocytochemistry/Immunofluorescence: TRP2 Antibody [NBP1-86893] - Mouse melanoblasts were incubated for 1h at 37C with anti-Trp2 rabbit 1:200, followed by incubation with Donkey anti-rabbit AF488. ICC/IF image submitted by a verified customer review.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86893] - Staining of human cerebral cortex, lymph node, skin and testis using Anti-TRP2 antibody NBP1-86893 (A) shows similar protein distribution across tissues to independent antibody NBP1-86892 (B).](http://images.novusbio.com/fullsize/TRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86893-img0015.jpg)
![Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86893] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/TRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86893-img0018.jpg)
![Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86893] - Staining of human skin shows cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/TRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86893-img0011.jpg)
![Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86893] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/TRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86893-img0013.jpg)
![Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86893] - Staining of human lymph node shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/TRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86893-img0017.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: TRP2 Antibody [NBP1-86893] - Analysis in human cell line SK-MEL-30 and human cell line U-2 OS.](http://images.novusbio.com/fullsize/TRP2-Antibody-Western-Blot-NBP1-86893-img0012.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - mouse kidney. FACL4 stained by FACL4 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16401-img0012.jpg "Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - mouse kidney. FACL4 stained by FACL4 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: FACL4 Antibody [NBP2-16401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16401-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16401] - analysis of FACL4 in MCF10A cells using anti-FACL4 antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunofluorescence-NBP2-16401-img0005.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Rat middle brain. FACL4 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16401-img0008.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Mouse lymph node. FACL4 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16401-img0009.jpg)
![Immunoprecipitation: FACL4 Antibody [NBP2-16401] - Immunoprecipitation of FACL4 protein from HeLa whole cell extracts using 5 ug of FACL4 antibody [C3], C-term Western blot analysis was performed using FACL4 antibody [C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunoprecipitation-NBP2-16401-img0007.jpg)
![FACL4 antibody [C3], C-term detects FACL4 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-immunohistochemistry-paraffin-261120251821100.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-261120251832454.jpg)
![FACL4 antibody [C3], C-term detects FACL4 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-immunocytochemistry-immunofluorescence-2611202518263624.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-26112025193395.jpg)
![FACL4 antibody [C3], C-term detects FACL4 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-immunohistochemistry-paraffin-261120251841135.jpg)
![HG causes changes in the expression of ferroptosis-related proteins in 661W cells. (A) Western blot analysis of ferroptosis-related protein expression levels in HG-induced 661W cells. GAPDH was used as a control. (B) Expression of GPX4 and SLC7A11 proteins was significantly downregulated in HG-stimulated 661W cells after 12, 18, and 24 h. HG induced obvious upregulation in the expression of ACSL4, FTH1, and NCOA4 in 661W cells compared with the Ctrl group. (C) Immunofluorescence staining of localization of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after 18 h. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01, *** p < 0.001 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-13220261739389.jpg)
![Fer-1 treatment attenuated changes in ferroptosis-related proteins’ expression in HG-stimulated 661W cells after 18 h. (A) Western blot analysis of ferroptosis-related proteins’ expression levels in HG-induced 661W cells after Fer-1 treatment. GAPDH was used as a control. (B) The downregulation in GPX4 and SLC7A11 protein expression in HG-stimulated 661W cells was significantly attenuated after Fer-1 treatment. The upregulation in ACSL4, FTH1, and NCOA4 protein expression in HG-stimulated 661W cells was effectively abrogated after Fer-1 treatment. (C) Immunofluorescence staining of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after Fer-1 administration. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-132202617595722.jpg)
![Fer-1 administration attenuated changes of ferroptosis-related proteins expression in diabetic mice retinas. (A) Western blot analysis of ferroptosis-related proteins expression levels in diabetic mice retinas after Fer-1 treatment. GAPDH was used as a control. (B) The decrease in GPX4 and SLC7A11 protein expression in diabetic mice retinas was significantly attenuated after Fer-1 treatment. The increase in ACSL4, FTH1, and NCOA4 protein expression in diabetic mice retinas was effectively abrogated after Fer-1 treatment. (C) Immunofluorescence staining of ferroptosis-related proteins (red) and nuclear (blue) in diabetic mice retinas after Fer-1 treatment: ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL), inner segment (IS), outer segment (OS). Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-13220261755394.jpg)
![HG causes changes in the expression of ferroptosis-related proteins in 661W cells. (A) Western blot analysis of ferroptosis-related protein expression levels in HG-induced 661W cells. GAPDH was used as a control. (B) Expression of GPX4 and SLC7A11 proteins was significantly downregulated in HG-stimulated 661W cells after 12, 18, and 24 h. HG induced obvious upregulation in the expression of ACSL4, FTH1, and NCOA4 in 661W cells compared with the Ctrl group. (C) Immunofluorescence staining of localization of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after 18 h. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01, *** p < 0.001 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-immunocytochemistry-immunofluorescence-132202619213323.jpg)
![Western Blot: Noxa Antibody [NB100-56368] - Noxa expression was stably reduced in HCT116 cells by shRNA. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Noxa-Antibody-Western-Blot-NB100-56368-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Noxa Antibody [NB100-56368] - Noxa expression was stably reduced in HCT116 cells by shRNA. Western blot image submitted by a verified customer review.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Noxa Antibody [NB100-56368] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using Noxa antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Noxa-Antibody-Immunohistochemistry-Paraffin-NB100-56368-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Noxa Antibody [NB100-56368] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Noxa (NB100-56368) antibody at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Noxa-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56368-img0003.jpg)
![Western Blot: Noxa Antibody [NB100-56368] - Western blot analysis of Noxa in A) human, B) mouse, and C) rat thymus tissue using Noxa antibody NB100-56368 at 2 ug/mL.](http://images.novusbio.com/fullsize/Noxa-Antibody-Western-Blot-NB100-56368-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0001.jpg "Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0006.jpg)
![Western Blot: Thioredoxin Antibody [NBP2-52983] - Total protein from human HeLa cells, mouse 3T3 cells and rat PC12 cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um pore size PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Thioredoxin in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Western-Blot-NBP2-52983-img0003.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-52983] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse intestine using rabbit polyclonal antibody against Thioredoxin (1:300 dilution). The signal was developed with HRP-secondary -DAB method and the sections were counter-stained using hematoxylin. The staining was found primarily in the epithelial cells of the intestinal lumen with weak to negligible signal in the basal or muscular layers. Isolated epithelial cells depicted nuclear positivity also.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-52983-img0002.jpg)
![Flow Cytometry: Thioredoxin-1 Antibody [NBP2-52983] - An intracellular stain was performed on HeLa cells with Thioredoxin-1 Antibody NBP2-52983AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-Flow-Cytometry-NBP2-52983-img0007.jpg)
![Flow Cytometry: Thioredoxin-1 Antibody [NBP2-52983] - Thioredoxin Antibody [NBP2-52983] - An intracellular stain was performed on U-87 MG cells with Thioredoxin Antibody NBP2-52983 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Flow-Cytometry-NBP2-52983-img0004.jpg)
![Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-8F4-Immunohistochemistry-Frozen-NBP1-51148-img0001.jpg "Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0009.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar pattern to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89284] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89284-img0007.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum shows moderate positivity in mitochondria in Purkinje cells.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0014.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89284] - ACAT1 porcine cerebral cortex. Image from verified customer review.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0008.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0011.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0012.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0013.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0011.jpg "Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F9NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0008.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B in human prostate](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0010.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0007.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis of S100B (15F4BN) in human brain tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in human prostate tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B (15F9NB) in human brain.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0009.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) [Azide Free] [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0002.jpg)