| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64803-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35822-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37271-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06565SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-26393SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89534-25ul | |||
![Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Mouse anti Human Myeloperoxidase.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-2C7-Immunohistochemistry-Paraffin-NB100-64803-img0002.jpg "Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Mouse anti Human Myeloperoxidase.")
![Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - An intracellular stain was performed on Jurkat cells with Myeloperoxidase antibody (2C7) NB100-64803PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-2C7-Flow-Cytometry-NB100-64803-img0005.jpg)
![Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Staining of human peripheral blood granulocytes with Mouse anti Human myeloperoxidase following permeabilisation with Leucoperm.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-2C7-Flow-Cytometry-NB100-64803-img0003.jpg)
![Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Analysis using the FITC conjugate of NB100-64803. Staining of permeabilised human peripheral blood granulocytes.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-2C7-Flow-Cytometry-NB100-64803-img0004.jpg)
![Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Western-Blot-NBP1-97762-img0004.jpg "Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1 Antibody [NBP1-97762] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97762-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1 Antibody [NBP1-97762] - Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-97762-img0006.jpg)
![Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Rat Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Western-Blot-NBP1-97762-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with Tris-EDTA pH 9, HRP-Staining. Antibody at 1:500.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-97762-img0005.jpg)
![Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of Mouse liver, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PON1-Antibody-4X7X2-Western-Blot-NBP3-16187-img0005.jpg "Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of Mouse liver, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of HepG2 cells, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/PON1-Antibody-4X7X2-Western-Blot-NBP3-16187-img0006.jpg)
![Immunohistochemistry-Paraffin: PON1 Antibody (4X7X2) [NBP3-16187] - Immunohistochemistry of paraffin-embedded human liver using PON1 Rabbit mAb (NBP3-16187) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PON1-Antibody-4X7X2-Immunohistochemistry-Paraffin-NBP3-16187-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Rat liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16187_rabbit-pon1-mab-4x7x2-52202511392230.jpg)
![Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Mouse liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16187_rabbit-pon1-mab-4x7x2-5220251255594.jpg)
![Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Human liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16187_rabbit-pon1-mab-4x7x2-5220251242555.jpg)
![Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-6Y10M1-Western-Blot-NBP3-15667-img0003.jpg "Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-6Y10M1-Western-Blot-NBP3-15667-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Confocal imaging of HeLa cells using RXR alpha/NR2B1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-5220251731103.jpg)
![Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR alpha/NR2B1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of RXR alpha/NR2B1 across chromosome 19 (upper panel) and the genomic region encompassing ECH1, a representative gene enriched in RXR alpha/NR2B1 (lower panel).](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-52202517344269.jpg)
![Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR alpha/NR2B1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of RXR alpha/NR2B1 in the representative genomic region surrounding ECH1 gene.](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-52202517315534.jpg)
![Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using RXR alpha/NR2B1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-52202517352787.jpg)
![Immunohistochemistry: PTRF Antibody [NBP3-35822] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PTRF Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202415152875.jpg "Immunohistochemistry: PTRF Antibody [NBP3-35822] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PTRF Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: PTRF Antibody [NBP3-35822] - Immunofluorescence analysis of C6 cells using PTRF Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202415171113.jpg)
![Western Blot: PTRF Antibody [NBP3-35822] - Western blot analysis of various lysates using PTRF Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202415175297.jpg)
![Immunocytochemistry/ Immunofluorescence: PTRF Antibody [NBP3-35822] - Immunofluorescence analysis of U-2 OS cells using PTRF Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202414163015.jpg)
![Immunocytochemistry/ Immunofluorescence: PTRF Antibody [NBP3-35822] - Immunofluorescence analysis of L929 cells using PTRF Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202415152835.jpg)
![Immunohistochemistry: PTRF Antibody [NBP3-35822] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using PTRF Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202414123822.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of esophageal cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0007.jpg)
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Flow-Cytometry-NBP2-37271-img0005.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against human NT5E (AA: 30-250) recombinant protein. (Expected MW is 26.6 kDa)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0002.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0003.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of endometrial cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0006.jpg)
![ELISA: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-ELISA-NBP2-37271-img0001.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Western-Blot-NBP2-74332-img0002.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1.")
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74332-img0004.jpg)
![Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74332-img0005.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Western-Blot-NBP2-74332-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Sphingosine Kinase 1/SPHK1.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74332-img0003.jpg)
![Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74332-img0006.jpg)
![Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2682024158262.jpg "Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds")
![Western Blot: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: Huh7 cell lysate Lane 5: Hela cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32722) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-27820241631640.jpg)
![Immunohistochemistry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32722) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29820241492649.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunocytochemistry analysis of HepG2 cells labeling Pyruvate Carboxylase with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2920241023429.jpg)
![Flow Cytometry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Flow cytometric analysis of PC-12 cells labeling Pyruvate Carboxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32722, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29202416372196.jpg)
![Western Blot: GLUT9 Antibody [NBP1-06565] - Detection of GLUT9a in human kidney membrane prep.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Western-Blot-NBP1-06565-img0002.jpg "Western Blot: GLUT9 Antibody [NBP1-06565] - Detection of GLUT9a in human kidney membrane prep.")
![Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-06565] - IHC-P analysis mouse kidney section with GLUT9 antibody at 1:200 dilution. This antibody gave the expected staining of SLC2A9-L isoform in the tubular epithelial cells and the blood vessel endothelium.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-Paraffin-NBP1-06565-img0003.jpg)
![Western Blot: SCD Antibody [NBP1-26393] - Normal human liver.](http://images.novusbio.com/fullsize/SCD-Antibody-Western-Blot-NBP1-26393-img0001.jpg "Western Blot: SCD Antibody [NBP1-26393] - Normal human liver.")
![Immunocytochemistry/Immunofluorescence: SCD Antibody [NBP1-26393] - SCD staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://images.novusbio.com/fullsize/SCD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-26393-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: EPHX2 Antibody [NBP1-89534] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/EPHX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89534-img0007.jpg "Immunocytochemistry/Immunofluorescence: EPHX2 Antibody [NBP1-89534] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.")
![Immunohistochemistry-Paraffin: EPHX2 Antibody [NBP1-89534] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EPHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-89534-img0011.jpg)
![Immunohistochemistry-Paraffin: EPHX2 Antibody [NBP1-89534] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/EPHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-89534-img0009.jpg)
![Immunohistochemistry-Paraffin: EPHX2 Antibody [NBP1-89534] - Staining of human prostate shows no positivity in smooth muscle cells as expected.](http://images.novusbio.com/fullsize/EPHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-89534-img0010.jpg)
![P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunohistochemistry-paraffin-1411202518431319.jpg "<b>Orthogonal Strategies Validation. </b>P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Orthogonal Strategies Validation. </b>P2Y2 antibody [HL2801] detects P2Y2 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunocytochemistry-immunofluorescence-141120251830212.jpg)
![P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat small intestine.P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunohistochemistry-paraffin-141120251839418.jpg)
![P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunohistochemistry-paraffin-141120251839412.jpg)
![P2Y2 antibody [HL2801] detects P2Y2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunohistochemistry-paraffin-141120251839317.jpg)
![P2Y2 antibody [HL2801] detects P2Y2 protein by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:100.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunocytochemistry-immunofluorescence-141120251824331.jpg)
![P2Y2 antibody [HL2801] detects P2Y2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48734_rabbit-p2y2-mab-hl2801-immunohistochemistry-paraffin-1411202518334522.jpg)