| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-724SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24464SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13996-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91858-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43982 | |||
| BSA Free | NBP2-61415-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92814-0.02ml | |||
![Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-724-img0004.jpg "Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NB100-724] - Analysis of a FFPE tissue section of human lymph node using 1:200 dilution of CTGF/CCN2 antibody (NB100-724). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunohistochemistry-Paraffin-NB100-724-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - Analysis of astrocyte reactivity, the cytoskeleton & migration rate following treatment with TGF-beta 2 & CCN2/CTGF. (A) GFAP immunoreactivity (green) is increased in murine ON astrocytes following TGF-beta 2 & CCN2/CTGF treatment. Nuclei are stained with Dapi (blue) (B) Western Blot experiments analyses of alpha -actinin protein synthesis in murine ON astrocytes after the treatment with 1 ng/ml TGF-beta 2, 50 ng/ml CCN2/CTGF & 100 ng/ml CCN2/CTGF for 24 h. Densitometric analyses of Western Blot analyses shows the significant increase in alpha -actinin protein synthesis. Mean value of untreated cells (control) was set at 1. GAPDH was used to normalize protein intensity (n = 4; 50 ng CCN2/CTGF: *p = 0.029; 100 ng CCN2/CTGF: *p = 0.015; unpaired two-tailed t-test). (C) Scratch assay analyses show a significant increased migration rate for murine ON astrocytes after the treatment with ng/ml TGF-beta 2, 50 ng/ml CCN2/CTGF & 100 ng/ml CCN2/CTGF for 12 h. Mean value of migration rate of control cells was set to 100% (control: n = 15; TGF-beta 2: n = 12, **p = 0.008; 50 ng/ml CCN2/CTGF: n = 10, *p = 0.001; 100 ng/ml CCN2/CTGF: n = 4, **p = 0.0001). Data represented as mean ± SD. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-724_rabbit-polyclonal-ctgf-ccn2-antibody-31020241683912.jpg)
![Immunohistochemistry: CTGF/CCN2 Antibody [NB100-724] - GFAP expression & synthesis in the ON & ONH in a murine glaucoma model. (A) GFAP immunoreactivity in the glial lamina of 1-month (green, upper panel) & 2 months (green, middle & lower panel) old beta B1-CTGF1 mice compared to WT controls. Immunoreactivity of GFAP not altered in 1-month old TG & WT animals (green, upper panel). 2 month old beta B1-CTGF1 mice showed an increased GFAP immunoreactivity compared to WT control (green, middle panel). Lower panel shows a magnified detail of the middle panel. Nuclei are stained w/ Dapi (blue). (B) Schmatic illustration of the optic nerve. A depicts region in the glial lamina where cross sections obtained. ONH (unmyelinated part) & ON (myelinated part) used for molecular analysis. (C) Quantification of immunohistochemical staining of GFAP in the glial lamina is not altered in 1 month old TG & WT animals (WT: n = 5; TG: n = 4). GFAP immunoreactivity is increased in 2 month old TG animals compared to WT littermates (WT: n = 10, TG: n = 13; *p = 0.039; two-tailed t-test compared to oretical mean of 1 (normalized control)). Mean value of WT animals (control) set at 1. (D) RT-PCR analyses revealed no alteration in the Gfap mRNA expression in the ON of 1-month (WT: n = 7; TG: n = 5) & 2 month old beta B1-CTGF1 mice (WT: n = 15; TG: n = 16) compared to WT controls. In the ONH the Gfap mRNA expression is increased in the 2 month old TG compared to WT animals (WT: n = 6, TG: n = 5; *p = 0.04). The mRNA expression of Gfap is not altered in 1 month old animals (WT: n = 6, TG: n = 5). Mean value of WT animals (control) set at 1. RPL32 used to normalize mRNA expression. Data represented as mean ± SEM. PRL, Prelaminar region; PSL, Postlaminar region; ON, optic nerve; ONH, optic nerve head. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-724_rabbit-polyclonal-ctgf-ccn2-antibody-310202415523931.jpg)
![Immunohistochemistry: CTGF/CCN2 Antibody [NB100-724] - GFAP expression & synthesis in the ON & ONH in a murine glaucoma model. (A) GFAP immunoreactivity in the glial lamina of 1-month (green, upper panel) & 2 months (green, middle & lower panel) old beta B1-CTGF1 mice compared to WT controls. Immunoreactivity of GFAP not altered in 1-month old TG & WT animals (green, upper panel). 2 month old beta B1-CTGF1 mice showed an increased GFAP immunoreactivity compared to WT control (green, middle panel). Lower panel shows a magnified detail of the middle panel. Nuclei are stained w/ Dapi (blue). (B) Schmatic illustration of the optic nerve. A depicts region in the glial lamina where cross sections obtained. ONH (unmyelinated part) & ON (myelinated part) used for molecular analysis. (C) Quantification of immunohistochemical staining of GFAP in the glial lamina is not altered in 1 month old TG & WT animals (WT: n = 5; TG: n = 4). GFAP immunoreactivity is increased in 2 month old TG animals compared to WT littermates (WT: n = 10, TG: n = 13; *p = 0.039; two-tailed t-test compared to oretical mean of 1 (normalized control)). Mean value of WT animals (control) set at 1. (D) RT-PCR analyses revealed no alteration in the Gfap mRNA expression in the ON of 1-month (WT: n = 7; TG: n = 5) & 2 month old beta B1-CTGF1 mice (WT: n = 15; TG: n = 16) compared to WT controls. In the ONH the Gfap mRNA expression is increased in the 2 month old TG compared to WT animals (WT: n = 6, TG: n = 5; *p = 0.04). The mRNA expression of Gfap is not altered in 1 month old animals (WT: n = 6, TG: n = 5). Mean value of WT animals (control) set at 1. RPL32 used to normalize mRNA expression. Data represented as mean ± SEM. PRL, Prelaminar region; PSL, Postlaminar region; ON, optic nerve; ONH, optic nerve head. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-724_rabbit-polyclonal-ctgf-ccn2-antibody-310202415535150.jpg)
![Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - Analyses of ECM components in murine ON astrocytes following treatment w/ TGF-beta 2 & CCN2/CTGF. (A) Immunocytochemical staining against fibronectin (green, upper panel) showed a markedly increase following the treatment w/ 1 ng/ml TGF-beta 2 or 50 ng/ml CCN2/CTGF. Immunocytochemical staining against tropoelastin (green, lower panel) showed a pronounced increase after treatment w/ 1 ng/ml TGF-beta 2 or 50 ng/ml CCN2/CTGF. Nuclei stained w/ Dapi (blue). (B) Real-time RT-PCR analyses shows an intense upregulation of collagen 3 alpha 1, tropoelastin & fibronectin mRNA in murine ON astrocytes after treatment w/ 1 ng/ml TGF-beta 2, 50 ng/ml CCN2/CTGF or 100 ng/ml CCN2/CTGF for 24 h compared to untreated control cells (collagen 3 alpha 1: n = 3; TGF-beta 2 ***p = 0.0000004, 50 ngCTGF **p = 0.005, 100ngCTGF **p = 0.008; fibronectin: n = 6, TGF-beta 2 *p = 0.00004, 50 ng CTGF *p = 0.016, 100 ngCTGF *p = 0.05; elastin: n = 5, TGF-beta 2 *p = 0.026, 50 ng CTGF *0.04, 100 ng CTGF *p = 0.03). mRNA expression normalized to Gnb2l & mean value of control cells set at 1. (C) Western Blot analysis show an increase in protein synthesis of collagen 3 alpha 1, elastin & fibronectin in murine ON astrocytes after treatment w/ 1 ng/ml TGF-beta 2, 50 ng/ml CCN2/CTGF or 100 ng/ml CCN2/CTGF for 24 h compared to untreated control cells (collagen 3 alpha 1: n = 3, TGF-beta 2 *p = 0.03, 100 ngCTGF **p = 0.009; fibronectin: n = 5, **p = 0.005, 50 ngCTGF **p = 0.006, 100 ng CTGF *p = 0.02; tropoelastin: n = 5, TGF-beta 2 ***p = 0.0004, 50 ngCTGF **p = 0.002, 100 ngCTGF ***p = 0.00001). GAPDH & alpha -tubulin used to normalize protein synthesis & mean value of control cells set at 1. Right panel shows representative Western Blots for all three proteins. Data represented as mean ± SD. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-724_rabbit-polyclonal-ctgf-ccn2-antibody-310202415532092.jpg)
![Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - Increasing substratum stiffness causes alterations in murine ON astrocyte reactivity, actin cytoskeleton & CCN2/CTGF level. (A) Filamentous actin labeled by phalloidin (red, upper panel) is increased when murine ON astrocytes were cultured on substrates with higher stiffness (30 kPa, 60 kPa) compared to softer control (10 kPa). Increasing substratum stiffness leads to an enhanced formation of actin stress fibers. Immunoreactivity of CCN2/CTGF (green, middle panel) is markedly increased when murine ON astrocytes were grown on substrates with higher stiffness (30, 60 kPa) compared to softer control (10 kPa). Murine ON astrocytes show an enhanced GFAP protein synthesis (green, lower panel) when cultured on 30 or 60 kPa compared to 10 kPa. Nuclei were stained with Dapi (blue). (B) CCN2/CTGF protein synthesis is significantly increased in murine ON astrocytes grown on 60 kPa compared to 10 kPa (10 kPa: n = 5; 30 kPa: n = 5; 60 kPa: n = 5; *p = 0.04; unpaired two-tailed t-test). (C) Protein synthesis of GFAP (10 kPa: n = 6; 30 kPa: n = 6, **p = 0.005; 60 kPa: n = 4, *p = 0.04; unpaired two-tailed t-test) show an enhanced reactivity of murine ON astrocytes cultured on 30 kPa or 60 kPa compared to 10 kPa control. (D) Vimentin protein synthesis is significantly increased in murine ON astrocytes cultured on 60 kPa compared to 10 kPa (10 kPa: n = 4; 30 kPa: n = 4; 60 kPa: n = 3 ***p = 0.00002). Protein synthesis was normalized to alpha -tubulin & mean value of control (10 kPa) was set at 1. Data represented as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-724_rabbit-polyclonal-ctgf-ccn2-antibody-31020241612698.jpg)
![Western Blot: FAM3C Antibody [NBP2-24464] - Total protein from human testis tissue and mouse testis tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FAM3C in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/FAM3C-Antibody-NBP2-24464-img0012.jpg "Western Blot: FAM3C Antibody [NBP2-24464] - Total protein from human testis tissue and mouse testis tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FAM3C in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-24464] - Analysis of tissue section of mouse brain using FAM3C antibody at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-24464-img0010.jpg)
![Western Blot: FAM3C Antibody [NBP2-24464] - Analysis of FAM3C in human brain lysate (0.5 ug/ml) 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate (0.5 ug/ml), and 4) in rat brain lysate (0.5 ug/ml) using FAM3C antibody.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Western-Blot-NBP2-24464-img0008.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-24464] - Staining of FAM3C using FAM3C antibody in normal human brain at 5 ug/ml (using control rabbit Ig for figure B).](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-24464-img0006.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-24464] - Analysis of FAM3C in human skin using FAM3C antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-24464-img0007.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-24464] - Analysis tissue section of mouse brain using FAM3C antibody Lot A1 at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-24464-img0011.jpg)
![Simple Western: FAM3C Antibody [NBP2-24464] - Simple Western lane view shows a specific band for FAM3C in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Simple-Western-NBP2-24464-img0005.jpg)
![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NBP2-16025] - Human gastric cancer, using CTGF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-16025-img0007.jpg "Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NBP2-16025] - Human gastric cancer, using CTGF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: CTGF/CCN2 Antibody [NBP2-16025] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Western-Blot-NBP2-16025-img0011.jpg)
![Western Blot: FAM3C Antibody [NBP2-13996] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/FAM3C-Antibody-Western-Blot-NBP2-13996-img0009.jpg "Western Blot: FAM3C Antibody [NBP2-13996] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: FAM3C Antibody [NBP2-13996] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13996-img0005.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-13996] - Staining of human hippocampus shows strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-13996-img0001.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-13996] - Staining of human duodenum shows high expression.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-13996-img0006.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-13996] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-13996-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-13996] - Staining in human duodenum and skeletal muscle tissues using anti-FAM3C antibody. Corresponding FAM3C RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-13996-img0008.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-13996] - Staining of human cerebral cortex shows positivity in neurons.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-13996-img0010.jpg)
![Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-13996] - Staining of human placenta shows positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/FAM3C-Antibody-Immunohistochemistry-Paraffin-NBP2-13996-img0011.jpg)
![Immunohistochemistry-Paraffin: DDR2 Antibody [NBP2-14926] - Human breast cancer, using DDR2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/DDR2-Antibody-Immunohistochemistry-Paraffin-NBP2-14926-img0001.jpg "Immunohistochemistry-Paraffin: DDR2 Antibody [NBP2-14926] - Human breast cancer, using DDR2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Western Blot: DDR2 Antibody [NBP2-14926] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/DDR2-Antibody-Western-Blot-NBP2-14926-img0004.jpg)
![Western Blot: DDR2 Antibody [NBP2-14926] - U87-MG whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDR2-Antibody-Western-Blot-NBP2-14926-img0005.jpg)
![Western Blot: DDR2 Antibody [NBP2-14926] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-14926_rabbit-polyclonal-ddr2-antibody-91020242063328.jpg)
![DDR2 antibody [C2C3], C-term detects DDR2 protein at cell membrane by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DDR2 stained by DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:250.Red: Tau, an Axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-14926_rabbit-polyclonal-ddr2-antibody-immunocytochemistry-immunofluorescence-2611202518261315.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and DDR2 (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-14926_rabbit-polyclonal-ddr2-antibody-western-blot-2611202518384019.jpg)
![Western Blot: ECSIT Antibody [NBP1-91858] - Analysis in human cell lines PC-3 and HeLa using Anti-ECSIT antibody. Corresponding ECSIT RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/ECSIT-Antibody-Western-Blot-NBP1-91858-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: ECSIT Antibody [NBP1-91858] - Analysis in human cell lines PC-3 and HeLa using Anti-ECSIT antibody. Corresponding ECSIT RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ECSIT Antibody [NBP1-91858] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ECSIT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91858-img0010.jpg)
![Immunohistochemistry-Paraffin: ECSIT Antibody [NBP1-91858] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ECSIT-Antibody-Immunohistochemistry-Paraffin-NBP1-91858-img0008.jpg)
![Western Blot: ECSIT Antibody [NBP1-91858] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ECSIT-Antibody-Western-Blot-NBP1-91858-img0011.jpg)
![Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-CL5339-Western-Blot-NBP2-61415-img0007.jpg "Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Analysis in human cell line U-251 MG.")
![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of rat cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-CL5339-Immunohistochemistry-Paraffin-NBP2-61415-img0013.jpg)
![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of human cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-CL5339-Immunohistochemistry-Paraffin-NBP2-61415-img0008.jpg)
![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-CL5339-Immunohistochemistry-Paraffin-NBP2-61415-img0009.jpg)
![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of mouse cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-CL5339-Immunohistochemistry-Paraffin-NBP2-61415-img0010.jpg)
![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of mouse cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-CL5339-Immunohistochemistry-Paraffin-NBP2-61415-img0011.jpg)
![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of rat cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-CL5339-Immunohistochemistry-Paraffin-NBP2-61415-img0012.jpg)
![Western Blot: DDR2 Antibody (HL1107) [NBP3-13724] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DDR2-Antibody-HL1107-Western-Blot-NBP3-13724-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DDR2 Antibody (HL1107) [NBP3-13724] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: DDR2 Antibody (HL1107) [NBP3-13724] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DDR2-Antibody-HL1107-Western-Blot-NBP3-13724-img0002.jpg)
![Western Blot: DDR2 Antibody (HL1107) [NBP3-13724] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-91020242011494.jpg)
![<b>Orthogonal Strategies Validation. </b> Mouse tissues.DDR2 stained by DDR2 antibody [HL1107] diluted at 1:16000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-immunohistochemistry-paraffin-317202591535..png)
![DDR2 antibody [HL1107] detects DDR2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.DDR2 stained by DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:16000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-immunohistochemistry-paraffin-2611202518404824.jpg)
![U87-MG whole cell and membrane extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-western-blot-2611202518475722.jpg)
![DDR2 antibody [HL1107] detects DDR2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.DDR2 stained by DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:16000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-immunohistochemistry-paraffin-261120251843459.jpg)
![Western Blot: TCR gamma/delta Antibody (7A5) [NBP2-22510] - Analysis of 25 ug of CEM cell lysates.](http://images.novusbio.com/fullsize/TCR-gamma-delta-Antibody-7A5-Western-Blot-NBP2-22510-img0003.jpg "Western Blot: TCR gamma/delta Antibody (7A5) [NBP2-22510] - Analysis of 25 ug of CEM cell lysates.")
![Flow Cytometry: TCR gamma/delta Antibody (7A5) [NBP2-22510] - Analysis of TCR V gamma 9 in Jurkat cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/TCR-gamma-delta-Antibody-7A5-Flow-Cytometry-NBP2-22510-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NBP2-69990] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69990-img0004.jpg "Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NBP2-69990] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry: CTGF/CCN2 Antibody [NBP2-69990] - (6ug/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunohistochemistry-NBP2-69990-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NBP2-69990] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69990-img0003.jpg)
![Control: CTGF/CCN2 Antibody [NBP2-69990] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Control-NBP2-69990-img0001.jpg)
![Western Blot: CTGF/CCN2 Antibody [NBP2-16026] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Western-Blot-NBP2-16026-img0007.jpg "Western Blot: CTGF/CCN2 Antibody [NBP2-16026] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CTGF Antibody [NBP2-16026] - Sample (30 ug of whole cell lysate) A: HUVEC 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CTGF-Antibody-Western-Blot-NBP2-16026-img0001.jpg)
![Western Blot: ECSIT Antibody - BSA Free [NBP2-92814] - Western blot analysis of extracts of HepG2 cells, using ECSIT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92814_rabbit-polyclonal-ecsit-antibody-42202514251823.jpg%3Ft=1690982332 "Western Blot: ECSIT Antibody - BSA Free [NBP2-92814] - Western blot analysis of extracts of HepG2 cells, using ECSIT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] - Immunofluorescence analysis of L929 cells using ECSIT Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92814_rabbit-polyclonal-ecsit-antibody-42202514275299.jpg%3Ft=1690982383)
![Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] - Immunofluorescence analysis of U2OS cells using ECSIT Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92814_rabbit-polyclonal-ecsit-antibody-422025143047.jpg%3Ft=1690982390)
![Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] - Immunofluorescence analysis of H9C2 cells using ECSIT Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92814_rabbit-polyclonal-ecsit-antibody-422025143045.jpg%3Ft=1690982372)