| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22217SS | |||
| Azide and BSA Free | NBP2-80825 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | BC100-520SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97279-50ul | |||
![Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and LAMP2 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human LAMP-2/CD107b (H4B4) Monoclonal Antibody (Catalog # NBP2-22217) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for LAMP-2/CD107b at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Knockout-Validated-NBP2-22217-img0016.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and LAMP2 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human LAMP-2/CD107b (H4B4) Monoclonal Antibody (Catalog # NBP2-22217) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for LAMP-2/CD107b at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Immunofluorescence: [Alexa Fluor® 647] [NBP2-22217AF647] - T98G glioblastoma cells grown on #1.5 coverglass stained with a conjugated LAMP-2/CD107b (H4B4)AF647 antibody, counterstained with DAPI. Cathepsin D (green) staining also visible. This image was submitted via customer Review. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Immunocytochemistry-Immunofluorescence-NBP2-22217-img0015.jpg)
![Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis of LAMP-2/CD107b expression in Whole Cell Lysates: 2) HeLa, 3) Jurkat, 4) U937, 5) HepG2, Tissue Extracts: 6) Human kidney, 7) Human lung, 8) Human liver, and 9) Human placenta.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Western-Blot-NBP2-22217-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Staining of LAMP-2/CD107b in human liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Immunohistochemistry-Paraffin-NBP2-22217-img0001.jpg)
![Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - An intracellular stain was performed on U937 cells with HGF LAMP-2/CD107b [H4B4] Antibody NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Flow-Cytometry-NBP2-22217-img0017.jpg)
![Simple Western: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lane view shows a specific band for LAMP-2/CD107b in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Simple-Western-NBP2-22217-img0005.jpg)
![Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Localization of TMEM135 to the mitochondria. The mitochondrial fraction isolated from the WT mouse brain show TMEM135 signals by immunoblotting. Following proteins were used as organelle markers: MFN2-mitochondria; LAMP2-lysosome; Lamin B1- nucleus; PDI- endoplasmic reticulum (ER). Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19264), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Western-Blot-NBP2-22217-img0019.jpg)
![Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Representative pictures of effect of I/R on DNA damage, cell survival, and inflammation. Western blot analysis expression level of Cathepsin B (*P < 0.05) in total protein extract from RVA of I/R treated vs. control group. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphar.2018.01034/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Western-Blot-NBP2-22217-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LAMP-2/CD107b [NBP2-22217] at a 1:100 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Immunocytochemistry-Immunofluorescence-NBP2-22217-img0008.jpg)
![Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using anti-LAMP-2/CD107b (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Flow-Cytometry-NBP2-22217-img0004.jpg)
![ELISA: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Anti-LAMP-2/CD107b was used to assess lysates from human SVG-A cells via ELISA, by loading the indicated mass of total protein per well. Antibodies tested were diluted 1:250, and used in conjunction with an HRP-conjugated secondary antibody. Signal was detected by luminescence. Image from verified customer review.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-ELISA-NBP2-22217-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Analysis of LAMP-2/CD107b in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP-2/CD107b polyclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. LAMP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunocytochemistry-Immunofluorescence-NB300-591-img0012.jpg "Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Analysis of LAMP-2/CD107b in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP-2/CD107b polyclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. LAMP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.")
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Normal deparaffinized Human placenta tissue tissues stained with anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunohistochemistry-Paraffin-NB300-591-img0015.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues stained with anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunohistochemistry-Paraffin-NB300-591-img0016.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Analysis of 25ug of NRK cell lysates using anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Western-Blot-NB300-591-img0017.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Validation of subcellular fractionation and immunocytochemistry. RLC proteins are present at ER microsomes. Lysates from 7 DIV cortical neurons were subjected to differential centrifugation to isolate nuclear pellet (P1), postnuclear supernatant (PNS), mitochondrial pellet (P2), and ER microsomal pellet (P3), as depicted in the graphic on the right. Image collected and cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/embr.201744853) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Western-Blot-NB300-591-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP-2/CD107b polyclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunocytochemistry-Immunofluorescence-NB300-591-img0019.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Analysis of 10 ug of HeLa (left panel), NIH/3T3 (middle panel) and NRK (right panel) whole cell lysates using anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Western-Blot-NB300-591-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Analysis of LAMP-2/CD107b (green) in NIH/3T3 cells.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunocytochemistry-Immunofluorescence-NB300-591-img0013.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Both normal and cancer biopsies of deparaffinized Human tonsils were stained with anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunohistochemistry-Paraffin-NB300-591-img0014.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Exposure of rat primary microglial cells (rPMs) to combined antiretroviral therapy (cART) cocktail resulted in impaired lysosomal function. rPMs were seeded into six-well plates & treated with cART (5 µM each of tenofovir disoproxil fumarate (TDF), emtricitabine (FTC), & dolutegravir (DTG)) for the indicated time periods. (A,B) Exposure of microglia to cART resulted in a significant decrease in expression of lysosomal-associated membrane protein 2 (LAMP2) at 6 to 24 h post-treatment. (C) Representative bar graph showing cART-mediated significantly increased lysosomal membrane permeabilization (LMP) (24 h). (D,E) Microglia exposed to cART demonstrated a significant decrease in levels of mature cathepsin D (mCTSD) at 24 h post-treatment. (F) Representative bar graph showing exposure of cART significantly reduced the CTSD activity in rPMs (24 h). (G) Representative bar graph showing cART-mediated increased lysosomal pH in rPMs. (H,I) Acridine orange staining showing increased green color & reduced red color in cART-treated rPMs. H2O2 was used as a positive control for lysosome damage. Data is from three independent experiments. Actin beta (ACTB) served as a protein loading control for western blots. Data are expressed as means ± SEM & were analyzed using student t-test or one-way ANOVA. *, p < 0.05 vs. control; N.S., non-significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31569373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-591_rabbit-polyclonal-lamp-2-cd107b-antibody-310202416175213.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - HSPA overexpression abrogated cART-mediated impairment of lysosomal function. Control rPMs & heat shock protein family A (HSPA) overexpressing rPMs were seeded into six-well plates subjected to various treatments for 24 h. Protein homogenates were prepared for the detection of the indicated molecules. (A,B) Representative western blots showing overexpressing HSPA in rPMs reversed cART-mediated downregulation of LAMP2 & mCTSD expression levels. (C) Representative bar graph showing overexpression of HSPA in rPMs protected lysosomal pH. (D,E) Representative bar graphs showing HSPA protected LMP (D), & CTSD activity (E) in cART-treated rPMs. For all western blots, ACTB served as a protein loading control. Data is from three independent experiments & is expressed as means ± SEM & were analyzed using student t-test or one-way ANOVA. *, p < 0.05 vs. control; #, p < 0.05 vs. cART. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31569373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-591_rabbit-polyclonal-lamp-2-cd107b-antibody-31020241618933.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein. OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE and subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600-667 of isoform 1 of OS-9. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0019151), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OS9-Antibody-Western-Blot-BC100-520-img0004.jpg "Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein. OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE and subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600-667 of isoform 1 of OS-9. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0019151), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: OS9 Antibody [BC100-520] - OS9 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/OS9-Antibody-Immunocytochemistry-Immunofluorescence-BC100-520-img0002.jpg)
![Western Blot: OS9 Antibody [BC100-520] - WB analysis of OS9 in 1. A431 cell lysate, 2. HepG2 cell lysate, 3. MCF7 cell lysate and 4. 3T3 cell lysate.](http://images.novusbio.com/fullsize/OS9-Antibody-Western-Blot-BC100-520-img0001.jpg)
![Simple Western: OS9 Antibody [BC100-520] - Simple Western lane view shows a specific band for OS9 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/OS9-Antibody-Simple-Western-BC100-520-img0003.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482216.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415483621.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482293.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Cellular localization of OS-9 & PHD2.(A) A lectin gel-shift assay was conducted to test for glycosylated proteins. Total cell lysates of U2OS cells were incubated in the presence or absence of the endoglycosidases EndoH & PNGaseF for 6 h at 37°C. Digest products were separated on a reducing 10% SDS-PAGE gel which contained concanavalin A co-polymerized in the top layer of the separating gel to retard mobility of glycosylated proteins [56]. Glycosylated OS-9 is indicated as ‘g’, deglycosylated OS-9 as ‘d’. (B) Detection of OS-9 & PHD2 in the nuclear fraction. HEK293 cells with & without transfection of the plasmid pcDNA3-OS-9 were separated into nuclear fraction (N) & postnuclear supernatant (PS), the latter containing cytoplasm & organelles. Western blot analysis included BiP, GAPDH & lamin A as typical marker proteins for the ER, the cytoplasm & the nucleus, respectively. (C) Detection of OS-9 & PHD2 in the cytoplasm. HEK293 cells were co-transfected with pcDNA3-OS-9 & pPHD2-V5. For hypoxia, cells were exposed to 3% O2 for 4 h. Cells were treated with 50 µg/ml digitonin & centrifuged to obtain a cytoplasmic (C) & an organelle fraction (O) & subjected to immunoblotting. (D) Isolation of cellular endomembranes. HEK293 cells were lysed mechanically by several passages through a 30½G needle. The postnuclear supernatant was processed further by ultra-centrifugation to separate the organelles (O) from the cytosol (C). High salt treatment (1 M KCl) of the organelle fraction produced a wash fraction (W) that contained dissociated peripheral membrane proteins. For immunoblot analysis of subcellular fractionations, cell aliquots were normalized for cell number prior to loading (B–D). Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415483637.jpg)
![Western Blot: OS9 Antibody [BC100-520] - OS-9 shows no effect on regulation of HIF-1 alpha .Total cell lysates were used for SDS-PAGE & subsequent Western blotting. To generate nearly anoxic conditions, cells were exposed to an oxygen consuming chemical system or to 1% or 3% O2 for 4 h to generate hypoxia. (A) U2OS, HeLa & Hep3B cells were transiently transfected with the plasmid pOS-9-V5 48 h prior to the experiment. Lamin A & actin were used as loading controls. (B) U2OS cells were subjected to ER stress by incubation either with tunicamycin (1 µg/ml) or thapsigargin (0.5 µg/ml) for 20 h. To detect HIF-1 alpha under normoxia, cells were treated with DMOG (1 mM) for 4 h. A sample of DMSO-only treated cells was loaded to exclude unspecific side effects of the solvent. (C) U2OS cells were transduced with lentiviral construct pLKO.1-shRNA-OS-9 (shOS-9) mediating a stable knockdown of OS-9 expression. Control cells (c) were transduced with plasmid pLKO.1-puro. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482250.jpg)
![Western Blot: OS9 Antibody [NB100-519] - Contribution and interaction of OS-9 for degradation of SHH proteins. Cells stably expressing the N278A-HA precursor were depleted of OS-9 by siRNA, and the fate of N278A was followed after cycloheximide (CHX) addition. All samples were analyzed by SDS-PAGE and immunoblotting with HA antibodies. Controls were treated with a commercial negative control siRNA. The samples were also immunoblotted for OS-9 to show the extent of depletion. Immunoblotting with GAPDH antibodies served as loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092164) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OS9-Antibody-Knockdown-Validated-NB100-519-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: OS9 Antibody [NB100-519] - Contribution and interaction of OS-9 for degradation of SHH proteins. Cells stably expressing the N278A-HA precursor were depleted of OS-9 by siRNA, and the fate of N278A was followed after cycloheximide (CHX) addition. All samples were analyzed by SDS-PAGE and immunoblotting with HA antibodies. Controls were treated with a commercial negative control siRNA. The samples were also immunoblotted for OS-9 to show the extent of depletion. Immunoblotting with GAPDH antibodies served as loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092164) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: OS9 Antibody [NB100-519] - Detection of OS-9.1 and OS-9.2 in total HEK293 cells, HEK293 cells expressing OS-9.1 or OS-9.2 by western analysis.](http://images.novusbio.com/fullsize/OS9-Antibody-Western-Blot-NB100-519-img0005.jpg)
![Immunohistochemistry: OS9 Antibody [NB100-519] - Staining of OS9 in mouse smooth muscle.](http://images.novusbio.com/fullsize/OS9-Antibody-Immunohistochemistry-NB100-519-img0009.jpg)
![Immunoprecipitation: OS9 Antibody [NB100-519] - Immunoprecipitation of OS-9.1 and OS-9.2 in total HEK293 cells, HEK293 cells expressing OS-9.1 or OS-9.2.](http://images.novusbio.com/fullsize/OS9-Antibody-Immunoprecipitation-NB100-519-img0008.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0006.jpg "Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.")
![Western Blot: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of LAMP2a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human liver tissue lysateLane 2: JAR cell lysate Lane 3: human placenta tissue lysate Lane 4: human lung tissue lysate](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Western-Blot-NBP2-67298-img0007.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-LAMP-2/CD107b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0008.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human liver tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human pancreas tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse brain tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0005.jpg)
![Western Blot: LAMP-2/CD107b Antibody (5N8J3) [NBP3-15282] - Western blot analysis of extracts of various cell lines, using LAMP-2/CD107b Rabbit mAb (NBP3-15282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-5N8J3-Western-Blot-NBP3-15282-img0004.jpg "Western Blot: LAMP-2/CD107b Antibody (5N8J3) [NBP3-15282] - Western blot analysis of extracts of various cell lines, using LAMP-2/CD107b Rabbit mAb (NBP3-15282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (5N8J3) [NBP3-15282] - Immunohistochemistry of paraffin-embedded mouse kidney using LAMP-2/CD107b Rabbit mAb (NBP3-15282) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-5N8J3-Immunohistochemistry-Paraffin-NBP3-15282-img0002.jpg)
![Western Blot: LAMP-2/CD107b Antibody (5N8J3) [NBP3-15282] - Western blot analysis of extracts of A-549 cells, using LAMP-2/CD107b Rabbit mAb (NBP3-15282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-5N8J3-Western-Blot-NBP3-15282-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (5N8J3) [NBP3-15282] - Immunohistochemistry of paraffin-embedded rat kidney using LAMP-2/CD107b Rabbit mAb (NBP3-15282) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-5N8J3-Immunohistochemistry-Paraffin-NBP3-15282-img0001.jpg)
![Immunoprecipitation: LAMP-2/CD107b Antibody (5N8J3) [NBP3-15282] - Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug LAMP-2/CD107b antibody (NBP3-15282). Western blot was performed from the immunoprecipitate using LAMP-2/CD107b antibody (NBP3-15282) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-5N8J3-Immunoprecipitation-NBP3-15282-img0005.jpg)
![Immunohistochemistry: LAMP-2/CD107b Antibody (5N8J3) [LAMP-2/CD107b] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using LAMP-2/CD107b Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15282_rabbit-lamp-2-cd107b-mab-5n8j3-52202517235736.jpg)
![Immunohistochemistry: LAMP-2/CD107b Antibody (5N8J3) [LAMP-2/CD107b] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using LAMP-2/CD107b Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15282_rabbit-lamp-2-cd107b-mab-5n8j3-52202517251982.jpg)
![Immunohistochemistry: LAMP-2/CD107b Antibody (5N8J3) [LAMP-2/CD107b] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using LAMP-2/CD107b Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15282_rabbit-lamp-2-cd107b-mab-5n8j3-52202517251957.jpg)
![Western Blot: LAMP-2/CD107b Antibody (C9-9) [NBP2-66929] - Western blot analysis of LAMP-2/CD107b on Hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-C9-9-Western-Blot-NBP2-66929-img0006.jpg "Western Blot: LAMP-2/CD107b Antibody (C9-9) [NBP2-66929] - Western blot analysis of LAMP-2/CD107b on Hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (C9-9) [NBP2-66929] - ICC staining of LAMP2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-C9-9-Immunocytochemistry-Immunofluorescence-NBP2-66929-img0007.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (C9-9) [NBP2-66929] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-LAMP-2/CD107b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-C9-9-Immunohistochemistry-Paraffin-NBP2-66929-img0005.jpg)
![Flow Cytometry: LAMP-2/CD107b Antibody (C9-9) [NBP2-66929] - Flow cytometric analysis of LAMP-2/CD107b was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-C9-9-Flow-Cytometry-NBP2-66929-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (C9-9) [NBP2-66929] - ICC staining of LAMP-2/CD107b in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 Goat anti-mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-C9-9-Immunocytochemistry-Immunofluorescence-NBP2-66929-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (C9-9) [NBP2-66929] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LAMP-2/CD107b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-C9-9-Immunohistochemistry-Paraffin-NBP2-66929-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (C9-9) [NBP2-66929] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LAMP-2/CD107b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-C9-9-Immunohistochemistry-Paraffin-NBP2-66929-img0004.jpg)
![ELISA: Mouse Troponin I Type 3 (cardiac) Sandwich ELISA Optimization Kit [NBP3-00456] - Samples were spiked with high concentrations of Mouse Troponin I Type 3 (cardiac) and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00456_mouse-troponin-i-type-3-sandwich-elisa-optimization-kit-13220241612134.jpg "ELISA: Mouse Troponin I Type 3 (cardiac) Sandwich ELISA Optimization Kit [NBP3-00456] - Samples were spiked with high concentrations of Mouse Troponin I Type 3 (cardiac) and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Troponin I Type 3 (cardiac) Sandwich ELISA Optimization Kit [NBP3-00456] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00456_mouse-troponin-i-type-3-sandwich-elisa-optimization-kit-4320248574368.jpg)
![Immunohistochemistry-Paraffin: OS9 Antibody [NBP2-97279] - Staining of human OS9 in human pancreas with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/OS9-Antibody-Immunohistochemistry-Paraffin-NBP2-97279-img0003.jpg "Immunohistochemistry-Paraffin: OS9 Antibody [NBP2-97279] - Staining of human OS9 in human pancreas with rabbit polyclonal antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: OS9 Antibody [NBP2-97279] - Staining of human OS9 in human placenta with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/OS9-Antibody-Immunohistochemistry-Paraffin-NBP2-97279-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: OS9 Antibody [NBP2-97279] - Immunofluorescence staining of OS9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97279 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97279_rabbit-polyclonal-os9-antibody-235202521451795.png)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (GL2A7) [NBP1-97499] - Mouse astrocytes stained with Anti-LAMP-2/CD107b. Cells were fixed using paraformaldehyde-lysine-periodate (including 4% paraformaldehyde) and permeabilized using 0.01% saponin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-GL2A7-Immunocytochemistry-Immunofluorescence-NBP1-97499-img0008.jpg "Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (GL2A7) [NBP1-97499] - Mouse astrocytes stained with Anti-LAMP-2/CD107b. Cells were fixed using paraformaldehyde-lysine-periodate (including 4% paraformaldehyde) and permeabilized using 0.01% saponin.")