Mouse Troponin I Type 3 (cardiac) Sandwich ELISA Optimization Kit

ELISA: Mouse Troponin I Type 3 (cardiac) Sandwich ELISA Optimization Kit [NBP3-00456] - Samples were spiked with high concentrations of Mouse Troponin I Type 3 (cardiac) and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Troponin I Type 3 (cardiac) Sandwich ELISA Optimization Kit [NBP3-00456] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 7.81 - 500 pg/mL
• Sensitivity: 4.69 pg/mL

Mouse Troponin I Type 3 (cardiac) Sandwich ELISA Optimization Kit Summary

• Specificity: This kit recognizes Mouse Troponin I Type 3 (cardiac) in samples. No significant cross-reactivity or interference between Mouse Troponin I Type 3 (cardiac) and analogues was observed.
• Standard Curve Range: 7.81 - 500 pg/mL
• Sensitivity: 4.69 pg/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: 5.1%
• Intra-Assay: 4.59%
• Spike Recovery: 88 - 108%
• Sample Volume: 100 ul
• Kit Type: Sandwich ELISA Optimization Kit
• Gene: TNNI3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Troponin I Type 3 (cardiac) Sandwich ELISA Optimization Kit

• Cardiac troponin I
• CMD1FF
• CMD2A
• CMH7MGC116817
• CTnI
• familial hypertrophic cardiomyopathy 7
• RCM1
• TNNC1
• TNNC1troponin I, cardiac
• TNNI3,
• troponin I type 3 (cardiac)
• troponin I, cardiac muscle
• Troponin I/TNNI3

Background

Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Troponin I Type 3 (cardiac) ELISA Kit (NBP3-00456)(5)

Publications using NBP3-00456

• Feliz‐Mosquea Y, Wilson A, Cruz‐Diaz N et al. Dietary influences on chemotherapy sensitivity and cardiotoxicity modulated by IRE1 targeting in triple‐negative breast cancer in female mice Physiological Reports 2025-09-21 [PMID: 40976979]
• Del Nogal Avila M, Das R, Kharlyngdoh JB et al. Cytokine storm based mechanisms for extra-pulmonary manifestations of SARS-CoV-2 infection JCI insight 2023-04-11 [PMID: 37040185]
• Sosnowski DK, Jamieson KL, Gruzdev A et al. Cardiomyocyte-specific disruption of soluble epoxide hydrolase limits inflammation to preserve cardiac function American journal of physiology. Heart and circulatory physiology 2022-08-19 [PMID: 35985007] (ELISA, Mouse)
• Aragon-Sanabria V, Aditya A, Zhang L et al. Ultrasmall Nanoparticle Delivery of Doxorubicin Improves Therapeutic Index for High-Grade Glioma Clinical cancer research : an official journal of the American Association for Cancer Research 2022-05-02 [PMID: 35499557] (ELISA, Mouse)
• Liu y, Chu G, Shen W et al. XMU-MP-1 protects heart from ischemia/reperfusion injury in mice through modulating Mst1/AMPK pathway European journal of pharmacology 2022-03-15 [PMID: 35123978]

FAQs for Troponin I Type 3 (cardiac) ELISA Kit (NBP3-00456). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TNNI3