| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-78039SS | |||
| Azide and BSA Free | NBP2-80921 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | BC100-520SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58345SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76798SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87781-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48028 | |||
| Azide and BSA Free | NBP2-34637-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44740 | |||
| Azide and BSA Free | NBP2-34653-0.1mg | |||
![Western Blot: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Analysis of human tonsil (1), human spleen (2), and human lymph node (3) tissue using CD162 antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/Cutaneous-Lymphocyte-Antigen-CLA-Antibody-HECA-452-Western-Blot-NB100-78039-img0006.jpg "Western Blot: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Analysis of human tonsil (1), human spleen (2), and human lymph node (3) tissue using CD162 antibody at a concentration of 2 ug/ml.")
![Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Human tonsil tissue stained with PSGL-1/CD162 Antibody (HECA-452) at 5ug/mL, and Leica Refine DAB kit. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PSGL-1-CD162-Antibody-HECA-452-Immunohistochemistry-Paraffin-NB100-78039-img0014.jpg)
![Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD3+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a matched isotype control NBP2-31379. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems]. A co-stain was performed using CD3 antibody NBP2-25186APC.](http://images.novusbio.com/fullsize/Cutaneous-Lymphocyte-Antigen-CLA-Antibody-HECA-452-Flow-Cell-Surface-NB100-78039-img0008.jpg)
![Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast adenocarcinoma using CLA antibody (clone HECA-452) at 5 ug/ml concentration. The antibody generated a membrane-cytoplasmic staining in the cancerous cells of breast adenocarcinoma. Note:- Besides lymphocytes, CLA has been shown to be present on cells of normal endometrium as well as breast cancer tissues (PMID 19625313 and 22970241).](http://images.novusbio.com/fullsize/Cutaneous-Lymphocyte-Antigen-CLA-Antibody-HECA-452-Immunohistochemistry-Paraffin-NB100-78039-img0004.jpg)
![Flow Cytometry: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Human peripheral blood lymphocytes stained with purified HECA-452, followed by anti-mouse IgG FITC](http://images.novusbio.com/fullsize/Cutaneous-Lymphocyte-Antigen-CLA-Antibody-HECA-452-Flow-Cytometry-NB100-78039-img0005.jpg)
![Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD14+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a matched isotype control NBP2-31379. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by rat-Dylight 488 conjugated secondary antibody. A co-stain was performed using CD14 antibody NB100-77758AF488.](http://images.novusbio.com/fullsize/Cutaneous-Lymphocyte-Antigen-CLA-Antibody-HECA-452-Flow-Cell-Surface-NB100-78039-img0007.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein. OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE and subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600-667 of isoform 1 of OS-9. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0019151), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OS9-Antibody-Western-Blot-BC100-520-img0004.jpg "Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein. OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE and subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600-667 of isoform 1 of OS-9. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0019151), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: OS9 Antibody [BC100-520] - OS9 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/OS9-Antibody-Immunocytochemistry-Immunofluorescence-BC100-520-img0002.jpg)
![Western Blot: OS9 Antibody [BC100-520] - WB analysis of OS9 in 1. A431 cell lysate, 2. HepG2 cell lysate, 3. MCF7 cell lysate and 4. 3T3 cell lysate.](http://images.novusbio.com/fullsize/OS9-Antibody-Western-Blot-BC100-520-img0001.jpg)
![Simple Western: OS9 Antibody [BC100-520] - Simple Western lane view shows a specific band for OS9 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/OS9-Antibody-Simple-Western-BC100-520-img0003.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482216.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415483621.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482293.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Cellular localization of OS-9 & PHD2.(A) A lectin gel-shift assay was conducted to test for glycosylated proteins. Total cell lysates of U2OS cells were incubated in the presence or absence of the endoglycosidases EndoH & PNGaseF for 6 h at 37°C. Digest products were separated on a reducing 10% SDS-PAGE gel which contained concanavalin A co-polymerized in the top layer of the separating gel to retard mobility of glycosylated proteins [56]. Glycosylated OS-9 is indicated as ‘g’, deglycosylated OS-9 as ‘d’. (B) Detection of OS-9 & PHD2 in the nuclear fraction. HEK293 cells with & without transfection of the plasmid pcDNA3-OS-9 were separated into nuclear fraction (N) & postnuclear supernatant (PS), the latter containing cytoplasm & organelles. Western blot analysis included BiP, GAPDH & lamin A as typical marker proteins for the ER, the cytoplasm & the nucleus, respectively. (C) Detection of OS-9 & PHD2 in the cytoplasm. HEK293 cells were co-transfected with pcDNA3-OS-9 & pPHD2-V5. For hypoxia, cells were exposed to 3% O2 for 4 h. Cells were treated with 50 µg/ml digitonin & centrifuged to obtain a cytoplasmic (C) & an organelle fraction (O) & subjected to immunoblotting. (D) Isolation of cellular endomembranes. HEK293 cells were lysed mechanically by several passages through a 30½G needle. The postnuclear supernatant was processed further by ultra-centrifugation to separate the organelles (O) from the cytosol (C). High salt treatment (1 M KCl) of the organelle fraction produced a wash fraction (W) that contained dissociated peripheral membrane proteins. For immunoblot analysis of subcellular fractionations, cell aliquots were normalized for cell number prior to loading (B–D). Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415483637.jpg)
![Western Blot: OS9 Antibody [BC100-520] - OS-9 shows no effect on regulation of HIF-1 alpha .Total cell lysates were used for SDS-PAGE & subsequent Western blotting. To generate nearly anoxic conditions, cells were exposed to an oxygen consuming chemical system or to 1% or 3% O2 for 4 h to generate hypoxia. (A) U2OS, HeLa & Hep3B cells were transiently transfected with the plasmid pOS-9-V5 48 h prior to the experiment. Lamin A & actin were used as loading controls. (B) U2OS cells were subjected to ER stress by incubation either with tunicamycin (1 µg/ml) or thapsigargin (0.5 µg/ml) for 20 h. To detect HIF-1 alpha under normoxia, cells were treated with DMOG (1 mM) for 4 h. A sample of DMSO-only treated cells was loaded to exclude unspecific side effects of the solvent. (C) U2OS cells were transduced with lentiviral construct pLKO.1-shRNA-OS-9 (shOS-9) mediating a stable knockdown of OS-9 expression. Control cells (c) were transduced with plasmid pLKO.1-puro. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482250.jpg)
![Western Blot: OS9 Antibody [NB100-519] - Contribution and interaction of OS-9 for degradation of SHH proteins. Cells stably expressing the N278A-HA precursor were depleted of OS-9 by siRNA, and the fate of N278A was followed after cycloheximide (CHX) addition. All samples were analyzed by SDS-PAGE and immunoblotting with HA antibodies. Controls were treated with a commercial negative control siRNA. The samples were also immunoblotted for OS-9 to show the extent of depletion. Immunoblotting with GAPDH antibodies served as loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092164) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OS9-Antibody-Knockdown-Validated-NB100-519-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: OS9 Antibody [NB100-519] - Contribution and interaction of OS-9 for degradation of SHH proteins. Cells stably expressing the N278A-HA precursor were depleted of OS-9 by siRNA, and the fate of N278A was followed after cycloheximide (CHX) addition. All samples were analyzed by SDS-PAGE and immunoblotting with HA antibodies. Controls were treated with a commercial negative control siRNA. The samples were also immunoblotted for OS-9 to show the extent of depletion. Immunoblotting with GAPDH antibodies served as loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092164) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: OS9 Antibody [NB100-519] - Detection of OS-9.1 and OS-9.2 in total HEK293 cells, HEK293 cells expressing OS-9.1 or OS-9.2 by western analysis.](http://images.novusbio.com/fullsize/OS9-Antibody-Western-Blot-NB100-519-img0005.jpg)
![Immunohistochemistry: OS9 Antibody [NB100-519] - Staining of OS9 in mouse smooth muscle.](http://images.novusbio.com/fullsize/OS9-Antibody-Immunohistochemistry-NB100-519-img0009.jpg)
![Immunoprecipitation: OS9 Antibody [NB100-519] - Immunoprecipitation of OS-9.1 and OS-9.2 in total HEK293 cells, HEK293 cells expressing OS-9.1 or OS-9.2.](http://images.novusbio.com/fullsize/OS9-Antibody-Immunoprecipitation-NB100-519-img0008.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Proteins were extracted from control (Ctrl) and stretched (Stretch) 158N cells and western blot was performed for myelin protein PLP (26 kDa) following 20% of strain. Beta-Actin was used as a loading control. Results represent the mean +/- SEM of three independent experiments. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC6505516/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Western-Blot-NB100-74503-img0003.jpg "Western Blot: Myelin PLP Antibody [NB100-74503] - Proteins were extracted from control (Ctrl) and stretched (Stretch) 158N cells and western blot was performed for myelin protein PLP (26 kDa) following 20% of strain. Beta-Actin was used as a loading control. Results represent the mean +/- SEM of three independent experiments. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC6505516/) licensed under a CC-BY license.")
![Western Blot: Myelin PLP Antibody [NB100-74503] - Figure 1 shows a Western blot of PLP in adult mouse brain samples using NB100-74503. Figure 2 shows an immunocytochemical staining of PLP in mouse brain thick section using NB100-74503. Staining (m) is limited to myelin tracts and oligodendrocytes.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Western-Blot-NB100-74503-img0001.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Analysis of adult mouse brain samples.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Western-Blot-NB100-74503-img0002.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Alteration in myelin gene expression after in vitro mechanical tensile strain of 20% & 30%. Total RNA was extracted & RT-qPCR was performed in control (Ctrl) & stretched (Stretch) cells for myelin genes Mag (a, d), Cnp (b, e), & Plp (c, f, g, i) after strain of 20% (a, b, c, g) & 30% (d, e, f, i). Results represent the mean ± SEM of three independent experiments performed in triplicate. *p < 0.05; **p < 0.01. (h, j) Proteins were extracted from control (Ctrl) & stretched (Stretch) 158N cells & western blot was performed for myelin protein PLP (26 kDa) following 20% (h) & 30% (j) of strain. beta -Actin was used as a loading control. Results represent the mean ± SEM of three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30298339), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74503_rabbit-polyclonal-myelin-plp-antibody-310202415392571.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Alteration in myelin gene expression after in vitro mechanical tensile strain of 20% & 30%. Total RNA was extracted & RT-qPCR was performed in control (Ctrl) & stretched (Stretch) cells for myelin genes Mag (a, d), Cnp (b, e), & Plp (c, f, g, i) after strain of 20% (a, b, c, g) & 30% (d, e, f, i). Results represent the mean ± SEM of three independent experiments performed in triplicate. *p < 0.05; **p < 0.01. (h, j) Proteins were extracted from control (Ctrl) & stretched (Stretch) 158N cells & western blot was performed for myelin protein PLP (26 kDa) following 20% (h) & 30% (j) of strain. beta -Actin was used as a loading control. Results represent the mean ± SEM of three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30298339), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74503_rabbit-polyclonal-myelin-plp-antibody-310202415334915.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Mechanical tensile strain of 30% induces an elongation of paranodal junction & alteration in MAPK signaling in organotypic cerebellar slice culture. a Double immunostaining of organotypic cerebellar slices for CASPR in red & MAG in green. The response was evaluated at time 0 h post-stretch. Altered paranodal junctions are highlighted within white circles in high-magnification images. Scale bar, 50 μm; inset scale bar, 5 μm. b Sketch of CASPR & MAG labeling in the paranodal junction. Quantitative analysis is characterized by three length measurements: c nodal length called (A); d paranodal length called (B); & e (B-A) corresponding to the total length of the CASPR labeling. f–h Proteins were extracted following strain of 30% from control (Ctrl) & stretched (Stretch) slices & western blot was performed for the myelin protein MBP (f), ERK1/2 (g), & p38 (h). The ratio P-ERK on total ERK & the ratio P-p38 on total p38 are presented. beta -Actin was used as a loading control. Results represent the mean ± SEM (n = 3). ***p < 0.001. CASPR contactin-associated protein, MAG myelin-associated glycoprotein Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30298339), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74503_rabbit-polyclonal-myelin-plp-antibody-3102024168216.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Mechanical tensile strain of 30% induces an elongation of paranodal junction & alteration in MAPK signaling in organotypic cerebellar slice culture. a Double immunostaining of organotypic cerebellar slices for CASPR in red & MAG in green. The response was evaluated at time 0 h post-stretch. Altered paranodal junctions are highlighted within white circles in high-magnification images. Scale bar, 50 μm; inset scale bar, 5 μm. b Sketch of CASPR & MAG labeling in the paranodal junction. Quantitative analysis is characterized by three length measurements: c nodal length called (A); d paranodal length called (B); & e (B-A) corresponding to the total length of the CASPR labeling. f–h Proteins were extracted following strain of 30% from control (Ctrl) & stretched (Stretch) slices & western blot was performed for the myelin protein MBP (f), ERK1/2 (g), & p38 (h). The ratio P-ERK on total ERK & the ratio P-p38 on total p38 are presented. beta -Actin was used as a loading control. Results represent the mean ± SEM (n = 3). ***p < 0.001. CASPR contactin-associated protein, MAG myelin-associated glycoprotein Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30298339), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74503_rabbit-polyclonal-myelin-plp-antibody-31020241555233.jpg)
![Western Blot: Myelin PLP Antibody [NB100-74503] - Mechanical tensile strain of 30% induces an elongation of paranodal junction & alteration in MAPK signaling in organotypic cerebellar slice culture. a Double immunostaining of organotypic cerebellar slices for CASPR in red & MAG in green. The response was evaluated at time 0 h post-stretch. Altered paranodal junctions are highlighted within white circles in high-magnification images. Scale bar, 50 μm; inset scale bar, 5 μm. b Sketch of CASPR & MAG labeling in the paranodal junction. Quantitative analysis is characterized by three length measurements: c nodal length called (A); d paranodal length called (B); & e (B-A) corresponding to the total length of the CASPR labeling. f–h Proteins were extracted following strain of 30% from control (Ctrl) & stretched (Stretch) slices & western blot was performed for the myelin protein MBP (f), ERK1/2 (g), & p38 (h). The ratio P-ERK on total ERK & the ratio P-p38 on total p38 are presented. beta -Actin was used as a loading control. Results represent the mean ± SEM (n = 3). ***p < 0.001. CASPR contactin-associated protein, MAG myelin-associated glycoprotein Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30298339), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74503_rabbit-polyclonal-myelin-plp-antibody-3102024165743.jpg)
![Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NB110-58345-img0009.jpg "Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.")
![Immunohistochemistry: CDR2 Antibody [NB110-58345] - Purkinje neurons of the cerebellum.](http://images.novusbio.com/fullsize/CDR2-Antibody-Immunohistochemistry-NB110-58345-img0004.jpg)
![Flow Cytometry: CDR2 Antibody [NB110-58345] - Analysis using the DyLight 488 conjugate of NB110-58345. Thymus epithelial cells CDR2. Image from verified customer review.](http://images.novusbio.com/fullsize/CDR2-Antibody-Flow-Cytometry-NB110-58345-img0008.jpg)
![Western Blot: CDR2 Antibody [NB110-58345] - Analysis of CDR2 antibody on HeLa whole cell.](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NB110-58345-img0006.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-76798) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Knockout-Validated-NBP1-76798-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-76798) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-76798] - EL4 cell lysate with op18 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-76798] - Stathmin 1 antibody was tested in PC12 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76798-img0004.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP1-76798] - Human brain tissue with op18 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-76798-img0002.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining in human cerebral cortex and pancreas tissues using anti-PLP1 antibody. Corresponding PLP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining in human cerebral cortex and pancreas tissues using anti-PLP1 antibody. Corresponding PLP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Border of a demyelinated lesion. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0013.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0010.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0011.jpg)
![Western Blot: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Western-Blot-NBP2-76420-img0001.jpg "Western Blot: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of mouse CTCF by Simple Western™. Samples: Whole cell lysate (2.0 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (NBP2-76420) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-simple-western-303202311310.jpg)
![Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (NBP2-76420) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-simple-western-303202311923.jpg)
![Immunocytochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF in FFPE MCF-7 cells by ICC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunocytochemistry-NBP2-76420-img0003.jpg)
![Immunohistochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of mouse CTCF in FFPE renal cell carcinoma by IHC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunohistochemistry-NBP2-76420-img0004.jpg)
![Immunohistochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunohistochemistry-NBP2-76420-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) and rabbit anti-CTCF (NB500-177).](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunoprecipitation-NBP2-76420-img0005.jpg)
![Flow Cytometry-CTCF Antibody (BLR041F) [NBP2-76420] -Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] or isotype control (unshaded). Secondary: DyLight® 650-conjugatedgoat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-flow-cytometry-287202312246..jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. CRISPR knockout cell lines generated by Edigene](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-30883] - Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. CRISPR knockout cell lines generated by Edigene")
![<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-30883] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody (NBP1-30883). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-30883] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 protein stained by Stathmin 1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30883-img0007.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:H129912% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0003.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE Stathmin 1 antibody dilution: 1:10000](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0009.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 12 % SDS-PAGE Stathmin 1 antibody dilution: 1:2000](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-30883] - Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 protein stained by Stathmin 1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30883-img0006.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP1-30883] - Sample: Paraffin-embedded human breast carcinoma. Stathmin 1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-30883-img0005.jpg)
![Western Blot: CD79A Antibody (HM47/A9) [NBP2-44738] - Western Blot Analysis of Raji cell lysate using CD79A Antibody (HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Western-Blot-NBP2-44738-img0005.jpg "Western Blot: CD79A Antibody (HM47/A9) [NBP2-44738] - Western Blot Analysis of Raji cell lysate using CD79A Antibody (HM47/A9).")
![Immunocytochemistry/Immunofluorescence: CD79A Antibody (HM47/A9) [NBP2-44738] - Immunofluorescence Analysis of Raji cells labeling CD79a with CD79A Antibody (HM47/A9). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Immunocytochemistry-Immunofluorescence-NBP2-44738-img0003.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (HM47/A9) [NBP2-44738] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD79A Antibody (HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Immunohistochemistry-Paraffin-NBP2-44738-img0004.jpg)
![Flow Cytometry: CD79A Antibody (HM47/A9) [NBP2-44738] - Flow Cytometric Analysis of Raji cells. CD79A Antibody (HM47/A9) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Flow-Cytometry-NBP2-44738-img0006.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (HM47/A9) [NBP2-44738] - Formalin-fixed, paraffin-embedded human tonsil stained with CD79a antibody (HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Immunohistochemistry-Paraffin-NBP2-44738-img0001.jpg)
![Western Blot: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Western Blot Analysis of human Raji cell lysate using CD79A Antibody (JCB117 + HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Western-Blot-NBP2-34330-img0005.jpg "Western Blot: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Western Blot Analysis of human Raji cell lysate using CD79A Antibody (JCB117 + HM47/A9).")
![Immunocytochemistry/Immunofluorescence: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Immunofluorescent staining of paraformaldehyde-fixed Raji cells. CD79A Antibody (JCB117 + HM47/A9) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Immunocytochemistry-Immunofluorescence-NBP2-34330-img0004.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD79a MAb (JCB117 + HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Immunohistochemistry-Paraffin-NBP2-34330-img0001.jpg)
![Flow Cytometry: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Flow Cytometric Analysis of Raji cells. CD79A Antibody (JCB117 + HM47/A9) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Flow-Cytometry-NBP2-34330-img0006.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD79a MAb (JCB117 + HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Immunohistochemistry-Paraffin-NBP2-34330-img0002.jpg)