| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86168-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NB7570 | |||
| Pre-adsorbed | NB7569 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7354APC | |||
| Biotin | NB7354B | |||
| DyLight 350 | NB7354UV | |||
| DyLight 405 | NB7354V | |||
| DyLight 488 | NB7354G | |||
| DyLight 550 | NB7354R | |||
| DyLight 594 | NB7354DL594 | |||
| DyLight 650 | NB7354C | |||
| DyLight 680 | NB7354FR | |||
| DyLight 755 | NB7354IR | |||
| FITC | NB7356 | |||
| HRP | NB7357 | |||
| Janelia Fluor 525 | NB7354JF525 | |||
| Janelia Fluor 669 | NB7354JF669 | |||
| PE | NB7354PE | |||
| PerCP | NB7354PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7558APC | |||
| Biotin | NB7558B | |||
| DyLight 350 | NB7558UV | |||
| DyLight 405 | NB7558V | |||
| DyLight 488 | NB7558G | |||
| DyLight 550 | NB7558R | |||
| DyLight 594 | NB7558DL594 | |||
| DyLight 650 | NB7558C | |||
| DyLight 680 | NB7558FR | |||
| DyLight 755 | NB7558IR | |||
| FITC | NB7560 | |||
| HRP | NB7561 | |||
| Janelia Fluor 525 | NB7558JF525 | |||
| Janelia Fluor 669 | NB7558JF669 | |||
| PE | NB7558PE | |||
| PerCP | NB7558PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-79127-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56542SS | |||
| Azide and BSA Free | NBP2-33224 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-448SS | |||
![Immunocytochemistry/Immunofluorescence: CD14 Antibody [NBP1-86168] - Staining of human cell line SiHa shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86168-img0007.jpg "Immunocytochemistry/Immunofluorescence: CD14 Antibody [NBP1-86168] - Staining of human cell line SiHa shows localization to vesicles. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Analysis in human lymph node and pancreas tissues. Corresponding CD14 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human liver, lymph node, pancreas and rectum using Anti-CD14 antibody NBP1-86168 (A) shows similar protein distribution across tissues to independent antibody NBP1-86166 (B).](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0014.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human tonsil shows moderate membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0021.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human rectum shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0017.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0018.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0019.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human liver shows moderate to strong membranous positivity in hepatic sinusoid cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86168-img0020.jpg)
![Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Hepatic mincle (CLEC4E) in cirrhosis and ACLF. Representative Western blot showing protein levels of mincle in control, cirrhotic induced by bile-duct ligation (BDL), and rats with acute-on-chronic liver failure (ACLF) induced by lipopolysaccharide (LPS) within cirrhosis and quantification of the Western blots. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fimmu.2020.01352/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Western-Blot-NBP1-49311-img0022.jpg "Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Hepatic mincle (CLEC4E) in cirrhosis and ACLF. Representative Western blot showing protein levels of mincle in control, cirrhotic induced by bile-duct ligation (BDL), and rats with acute-on-chronic liver failure (ACLF) induced by lipopolysaccharide (LPS) within cirrhosis and quantification of the Western blots. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fimmu.2020.01352/full) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: CLEC4E Antibody (16E3) [NBP1-49311] - HeLa cells stained with monoclonal anti-human MINCLE antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Immunocytochemistry-Immunofluorescence-NBP1-49311-img0011.jpg)
![Immunohistochemistry-Paraffin: CLEC4E Antibody (16E3) [NBP1-49311] - Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human MINCLE antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Immunohistochemistry-Paraffin-NBP1-49311-img0010.jpg)
![Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - An intracellular stain was performed on U87MG cells with CLEC4E Antibody (16E3) NBP1-49311AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Flow-Cytometry-NBP1-49311-img0020.jpg)
![Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CLEC4E (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Western-Blot-NBP1-49311-img0009.jpg)
![Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - The Cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-human MINCLE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : CLEC4E transfected 293T cell lysate](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Western-Blot-NBP1-49311-img0017.jpg)
![Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Mincle (CLEC4E) in NASH. Representative western blot showing protein levels of mincle in NASH patients and non-NASH controls and quantification of the Western blots. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fimmu.2020.01352/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Western-Blot-NBP1-49311-img0021.jpg)
![Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - Flow cytometry analysis of CLEC4E in LNCap cell line,staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Flow-Cytometry-NBP1-49311-img0016.jpg)
![Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - An intracellular stain was performed on U-937 cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Flow-Cytometry-NBP1-49311-img0018.jpg)
![Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - An intracellular stain was performed on U87MG cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Flow-Cytometry-NBP1-49311-img0019.jpg)
![Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB7567] - Analysis of Jurkat cell lysate using anti-MBD1 antibody (Cat. # NB100-56537) followed by HRP conjugated Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NB7567-img0003.jpg "Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB7567] - Analysis of Jurkat cell lysate using anti-MBD1 antibody (Cat. # NB100-56537) followed by HRP conjugated Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the HRP form of this antibody.")
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - Analysis using Donkey anti-Goat IgG (H+L) Secondary Antibody using Embryo. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Western-Blot-NB7354-img0005.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - Analysis using Donkey anti-Goat IgG (H+L) Secondary Antibody using Embryo. Image using the HRP form of this antibody.")
![Immunohistochemistry-Paraffin: Donkey anti-Goat IgG (H+L) Secondary Antibody [NB7354] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:5,000 (0.2ug/ml). Secondary Antibody: Donkey anti-Goat IgG (H+L) Secondary Antibody HRP Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Immunohistochemistry-Paraffin-NB7354-img0004.jpg)
![Western Blot: Goat anti-Mouse IgG Fc Secondary Antibody [NB7558] - Analysis of Cadherin in Huh7 cell lysate. Image from verified customer review. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-Fc-Secondary-Antibody-Western-Blot-NB7558-img0001.jpg "Western Blot: Goat anti-Mouse IgG Fc Secondary Antibody [NB7558] - Analysis of Cadherin in Huh7 cell lysate. Image from verified customer review. Image using the HRP form of this antibody.")
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific BlueTM.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0007.jpg "Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific BlueTM.")
![Flow (Cell Surface): CD45 Antibody (HI30) [NBP1-79127] - hPBMC cells were stained with CD45 antibody (HI30) [NBP1-79127] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Flour 488.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cell-Surface-NBP1-79127-img0005.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific OrangeTM.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0008.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] purified, GAM-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0009.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Analysis of PE/Cy7 conjugate of CD45 antibody (HI30) [NBP1-79127]. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cy7 (blue histogram) or Anti-Human CD45 PE-Cy7 (purple histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0010.jpg)
![Flow (Cell Surface): CD45 Antibody (HI30) [NBP1-79127] - Flow (Cell Surface): Cell surface staining of PBMC's (Lymphocyte gate at 1 x 10^6 cells/ml) with CD45 antibody (HI30) [Alexa Fluor (R) 647] [NBP1-79127AF647] (orange) stained at a dilution of 1:500. Shown with mIgG1 (AF647 conjugated) Isotype control (blue). Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cell-Surface-NBP1-79127-img0011.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with NB500-452F (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0009.jpg "Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with NB500-452F (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to FITC.")
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Surface staining (mass cytometry) of hTERT cell line with anti-human CD105 (MEM-226) 143Nd. Gated on singlets.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0001.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Analysis using the FITC conjugate of NB500-452. Staining of HUVEC (human umbilical vein endothelial cells) with anti-human CD105 (MEM-226) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cell-Surface-NB500-452-img0003.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Surface staining of HUVEC (human umbilical vein endothelial cells) with anti-CD105 (MEM-226) PE. HEK-293 cells used as a negative control.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0004.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cell-Surface-NB500-452-img0005.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452AF647 (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0006.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452PCP (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0007.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Western-Blot-NB500-383-img0003.jpg "Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Staining of human peripheral blood with anti-human CD29 (MEM-101A) PE.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Flow-Cytometry-NB500-383-img0004.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) APC conjugated.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Flow-Cytometry-NB500-383-img0002.jpg)
![Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72793-img0005.jpg "Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.")
![Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - DyLight (R) dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-72793-img0011.jpg)
![Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4C. Block: incubated with blocking buffer for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72793-img0001.jpg)
![Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Mouse IgG1. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72793-img0002.jpg)
![Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Mouse IgG1. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72793-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Fluorescence absorption and emission spectra of Dylight 800 in PBS, pH 7.2.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP1-72793-img0006.jpg)
![Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72793-img0008.jpg)
![ELISA: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - ELISA results of purified Rabbit anti-Mouse IgG1 Heavy Chain Secondary antibody tested against purified Mouse IgG1. Each well was coated in duplicate with 1.0 ug of Mouse IgG1. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Goat anti-Rabbit HRP, and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-ELISA-NBP1-72793-img0012.jpg)
![Western Blot: IKK gamma Antibody (72C627) [NB100-56542] - Analysis using IKK gamma (NEMO) antibody. Lysate from 1) human Jurkat and 2) mouse NIH 3T3 cells probed with IKK gamma antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-72C627-Western-Blot-NB100-56542-img0005.jpg "Western Blot: IKK gamma Antibody (72C627) [NB100-56542] - Analysis using IKK gamma (NEMO) antibody. Lysate from 1) human Jurkat and 2) mouse NIH 3T3 cells probed with IKK gamma antibody at 2 ug/ml.")
![Simple Western: IKK gamma Antibody (72C627) [NB100-56542] - Simple Western lane view shows a specific band for IKK gamma in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-72C627-Simple-Western-NB100-56542-img0004.jpg)
![Western Blot: Derlin 1 Antibody [NB100-448] - Detection of Derlin protein in canine KRM microsomes.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Western-Blot-NB100-448-img0005.jpg "Western Blot: Derlin 1 Antibody [NB100-448] - Detection of Derlin protein in canine KRM microsomes.")
![Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NB100-448] - Derlin-1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-448-img0001.jpg)
![Immunohistochemistry: Derlin 1 Antibody [NB100-448] - Staining of human liver.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-NB100-448-img0006.jpg)
![Western Blot: TNF-alpha Antibody [NBP2-44062] - Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4C, DyLight 649 Goat anti-Rabbit added at 1:20,000 in blocking buffer for 30 min RT.](http://images.novusbio.com/fullsize/TNF-alpha Antibody-Western Blot-NBP2-44062-img0011.jpg "Western Blot: TNF-alpha Antibody [NBP2-44062] - Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4C, DyLight 649 Goat anti-Rabbit added at 1:20,000 in blocking buffer for 30 min RT.")
![Immunohistochemistry: TNF-alpha Antibody [NBP2-44062] - Human prostate tissue was stained using TNFa antibody. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Immunohistochemistry-NBP2-44062-img0010.jpg)
![Immunohistochemistry: TNF-alpha Antibody [NBP2-44062] - Fluorescent immunohistochemistry showing staining of human colon.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Immunohistochemistry-NBP2-44062-img0006.jpg)
![Immunohistochemistry: TNF-alpha Antibody [NBP2-44062] - Staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Immunohistochemistry-NBP2-44062-img0009.jpg)
