Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG, Fc Antibody [DyLight 488] [NBP1-72872] - analysis of Vero cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature. Image from ...read more
Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 488] Summary
Mouse IgG F(c) fragment
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab')2. This antibody will react with heavy chains of Mouse IgG. Minimal reactivity is expected against other Mouse immunoglobulins.
Immunogen affinity purified
Western Blot 1:10000
Fluorophore-linked immunosorbent assay 1:20000
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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