Goat anti-Rabbit IgG Fc Secondary Antibody [DyLight 405] (Pre-absorbed)

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Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG, Fc Antibody [DyLight 405] [NBP1-72958]

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, FLISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 405
Format
Pre-absorbed
Concentration
LYOPH

Goat anti-Rabbit IgG Fc Secondary Antibody [DyLight 405] (Pre-absorbed) Summary

Immunogen
Rabbit IgG Fc fragment
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 100 ul of deionized water.

Notes

Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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