Goat anti-Mouse IgG Fc Secondary Antibody

Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image from the DyLight 488 version of this antibody.

Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Analysis of Vero cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature. Image from verified customer review. Image using the DyLight 488 form of this antibody.

Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image using the DyLight 405 form of this antibody.

Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image using the DyLight 488 form of this antibody.

Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image using the DyLight 680 form of this antibody.

Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image using the DyLight 800 form of this antibody.

ELISA: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - ELISA results of Goat anti-Mouse IgG Fc Secondary antibodyy tested against purified MouseIgG Fc protein. Each well was coated in duplicate with 1.0 ug of MouseIgG Fc. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Donkey anti-Goat IgG Antibody Peroxidase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) and TMB substrate.

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Goat anti-Mouse IgG Fc Secondary Antibody Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum
  
  Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Goat anti-Mouse IgG Fc fragment Secondary Antibody was produced by repeated immunization with Mouse IgG F(c) fragment in goat.
• Specificity: No reaction was observed against Mouse IgG F(ab).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000 - 1:50000
  • Fluorophore-linked immunosorbent assay
  • Immunocytochemistry/ Immunofluorescence 1:5000
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:10000
• Application Notes: This product has been tested by ELISA, dot blot, and wester blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Fc Antibody (NBP2-60664)(2)

Publications using NBP2-60664

• Personius KE, Siebert D, Koch DW, Udin SB. Blockage of neuromuscular glutamate receptors impairs reinnervation following nerve crush in adult mice Frontiers in Cellular Neuroscience 2022-09-22 [PMID: 36212695] (Immunohistochemistry)
• Yacov N, Kafri P, Salem Y et al. MOSPD2 is a therapeutic target for the treatment of CNS inflammation Clin. Exp. Immunol. 2020-04-30 [PMID: 32353176]
  
  Details:
  Citation using the DyLight 680 format of this antibody.