Goat anti-Mouse IgG Fc Secondary Antibody [Texas Red]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Texas Red
Concentration
LYOPH

Goat anti-Mouse IgG Fc Secondary Antibody [Texas Red] Summary

Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum
Immunogen
Goat anti-Mouse IgG Fc fragment Secondary Antibody was produced by repeated immunization with Mouse IgG F(c) fragment in goat.
Specificity
No reaction was observed against Mouse IgG F(ab).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Multi-step

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-73434 in the following applications:

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Mouse IgG, Fc Antibody (NBP1-73434) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Canine.

Reviews using NBP1-73434:
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ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry IgG Fc NBP1-73434
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5
reviewed by:
Verified Customer
ICC Canine 09/11/2017
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Summary

ApplicationImmunocytochemistry
Sample Testedblood slides
SpeciesCanine
Lot2881

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Video Protocols

ICC/IF Video Protocol

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Verified Customer
09/11/2017
Application: ICC
Species: Canine