| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54582-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54790SS | |||
| Azide and BSA Free | NBP2-80644 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45346 | |||
| Azide and BSA Free | NBP2-34520-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-152SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-24913 | |||
| BSA Free | NB100-56744SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36491SS | |||
| Azide and BSA Free | NBP2-80662 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80038-50ug | |||
![ELISA: Transglutaminase 2/TGM2 Assay Kit (Colorimetric) [NBP1-37008] - Standard Curve image from a verified customer review.](http://images.novusbio.com/fullsize/nbp1-37008_transglutaminase-2-tgm2-assay-kit-colorimetric-elisa-2152025103730..jpg "ELISA: Transglutaminase 2/TGM2 Assay Kit (Colorimetric) [NBP1-37008] - Standard Curve image from a verified customer review.")
![Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Western-Blot-NBP2-67473-img0010.jpg "Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunocytochemistry-Immunofluorescence-NBP2-67473-img0008.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded mouse liver tissue using anti-TrkA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunohistochemistry-Paraffin-NBP2-67473-img0006.jpg)
![Flow Cytometry: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of SH-SY-5Y cells with TrkA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Flow-Cytometry-NBP2-67473-img0001.jpg)
![Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on different lysates using anti-TrkA antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: SH-SY-5Y](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Western-Blot-NBP2-67473-img0007.jpg)
![Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Western blot analysis of TrkA on N2A cell lysates using anti-TrkA antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Western-Blot-NBP2-67473-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunocytochemistry-Immunofluorescence-NBP2-67473-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunocytochemistry-Immunofluorescence-NBP2-67473-img0003.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded human tonsil tissue using anti-TrkA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunohistochemistry-Paraffin-NBP2-67473-img0004.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded mouse kidney tissue using anti-TrkA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunohistochemistry-Paraffin-NBP2-67473-img0005.jpg)
![Western Blot: Goat anti-Rat IgG (H+L) Secondary Antibody [NB7112] - Detection of DDK in SUSD3 overexpression lysate using anti-DYKDDDDK Epitope Tag antibody (cat.# NBP1-06712) followed by Goat anti-Rat IgG (H+L) Secondary Antibody HRP conjugated secondary antibody. Image from verified customer review. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgG-H+L-Secondary-Antibody-Western-Blot-NB7112-img0003.jpg "Western Blot: Goat anti-Rat IgG (H+L) Secondary Antibody [NB7112] - Detection of DDK in SUSD3 overexpression lysate using anti-DYKDDDDK Epitope Tag antibody (cat.# NBP1-06712) followed by Goat anti-Rat IgG (H+L) Secondary Antibody HRP conjugated secondary antibody. Image from verified customer review. Image using the HRP form of this antibody.")
![Immunocytochemistry/Immunofluorescence: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Immunofluorescence staining of Ramos cells using CD27/TNFRSF7 Antibody (LPFS2/1611) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunocytochemistry-Immunofluorescence-NBP2-53196-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Immunofluorescence staining of Ramos cells using CD27/TNFRSF7 Antibody (LPFS2/1611) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.")
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Formalin-fixed, paraffin-embedded human Colon stained with CD27/TNFRSF7 Antibody (LPFS2/1611).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunohistochemistry-Paraffin-NBP2-53196-img0006.jpg)
![Flow Cytometry: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Flow Cytometric Analysis of Ramos cells using CD27/TNFRSF7 Mouse Monoclonal Antibody (LPFS2/1611) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Flow-Cytometry-NBP2-53196-img0008.jpg)
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD27 Monoclonal Antibody (LPFS2/1611).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunohistochemistry-Paraffin-NBP2-53196-img0001.jpg)
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD27/TNFRSF7 Antibody (LPFS2/1611).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunohistochemistry-Paraffin-NBP2-53196-img0004.jpg)
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Formalin-fixed, paraffin-embedded human Spleen stained with CD27/TNFRSF7 Antibody (LPFS2/1611).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunohistochemistry-Paraffin-NBP2-53196-img0005.jpg)
![Protein Array: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CD27/TNFRSF7 Antibody (LPFS2/1611) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Protein-Array-NBP2-53196-img0007.jpg)
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0023.jpg "Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: CD36 Antibody (1283D) [NBP2-54790] - CD36 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human CD36/SR-B3 Monoclonal Antibody for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocyte membranes.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunohistochemistry-Paraffin-NBP2-54790-img0002.jpg)
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Total protein from human skin and adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-CD36 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD36 Antibody (1283D) [NBP2-54790] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-CD36 (1283D) at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective..](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunocytochemistry-Immunofluorescence-NBP2-54790-img0003.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-3102024161365.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114783.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416121990.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-31020241612242.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114740.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114741.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - CD36-mediated caveolar endocytosis transports FAs into cells.a–c 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated PacFA (50 μM) for 20 min, followed by UV crosslinking on ice for 30 min. a, b Cells were subjected to click chemistry using an N3-Alexa Fluro 488, & immunostained with anti-CD36 antibody. Colocalization of PacFA & CD36 was quantified from 24 cells over three independent experiments & plotted in (b). The value represents mean ± SEM. c Cells were lysed & subjected to click chemistry assay using N3-biotin. PacFA-labeled proteins were captured with streptavidin beads & subjected to western blot using anti-CD36 & anti-FABP4 antibodies. d WT, Cav1−/−, Cd36−/−, & Cav1−/−;Cd36−/− SVFs were isolated & differentiated into adipocytes & treated with 100 μM 3H-oleate (specific activity, 2268 dpm/nmol) for 1 h. Lipid fractions were extracted from the cells & subjected to scintillation counting. The radioactive counting was normalized to protein content. Each value represents mean ± SEM obtained from three samples. Two-sided Student’s t test was performed between WT & each of the knockout cells. These experiments were repeated twice. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416113520.jpg)
![Western Blot: CD44 Antibody (156-3C11) [NBP2-34327] - Western Blot Analysis of HeLa cell lysate using CD44 Antibody (156-3C11).](http://images.novusbio.com/fullsize/CD44-Antibody-156-3C11-Western-Blot-NBP2-34327-img0007.jpg "Western Blot: CD44 Antibody (156-3C11) [NBP2-34327] - Western Blot Analysis of HeLa cell lysate using CD44 Antibody (156-3C11).")
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (156-3C11) [NBP2-34327] - Confocal immunofluorescence image of HeLa cells using CD44 Antibody (156-3C11) Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/CD44-Antibody-156-3C11-Immunocytochemistry-Immunofluorescence-NBP2-34327-img0006.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) [NBP2-34327] - Formalin-paraffin human breast carcinoma stained with CD44 MAb (156-3C11)](http://images.novusbio.com/fullsize/CD44-Antibody-156-3C11-Immunohistochemistry-Paraffin-NBP2-34327-img0003.jpg)
![Flow Cytometry: CD44 Antibody (156-3C11) [NBP2-34327] - A surface stain was performed on HeLa cells with CD44 Antibody (156-3C11) NBP2-34520F and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to FITC. Image using the FITC format of this antibody.](http://images.novusbio.com/fullsize/CD44-Antibody-156-3C11-Flow-Cytometry-NBP2-34327-img0008.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) [NBP2-34327] - Formalin-fixed, paraffin-embedded human tonsil stained with CD44 MAb (156-3C11)](http://images.novusbio.com/fullsize/CD44-Antibody-156-3C11-Immunohistochemistry-Paraffin-NBP2-34327-img0001.jpg)
![Western Blot: Cathepsin E Antibody [NB400-152] - Detection of Cathepsin E in 10 ug of murine reticulocyte lysate using NB400-152.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Western-Blot-NB400-152-img0005.jpg "Western Blot: Cathepsin E Antibody [NB400-152] - Detection of Cathepsin E in 10 ug of murine reticulocyte lysate using NB400-152.")
![Immunohistochemistry: Cathepsin E Antibody [NB400-152] - Analysis of Cathepsin E in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Immunohistochemistry-NB400-152-img0006.jpg)
![Western Blot: Cathepsin E Antibody [NB400-152] - Analysis of Cathepsin E on mouse stomach lysate.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Western-Blot-NB400-152-img0001.jpg)
![Western Blot: Cathepsin E Antibody [NB400-152] - The expression & methylation status of Ctse gene in CD4+ T cells isolated from MRL/lpr lupus-prone (MRL) & C57BL/6 (B6) mice. (A) mRNA expression of Ctse in CD4+, CD8+, B cells & macrophages in B6 & MRL mice. ***p < 0.001. (B) Western blot analyses of CTSE in CD4+, CD8+, B cells & macrophages in B6, MRL & mice. (C) Methylated region expanding 583 bp in Ctse intron 1 in B6 mice demonstrated by UCSC Genome Browser. In MRL mice, 583 bp region is hypomethylated. Duplicate reads are indicated by arrows. (D) Nucleotide sequence within 583 bp flanking region of Ctse intron 1 in B6 mice. Among 13 CpGs (red & underlined), 11th CpG (boxed) includes mCGCG binding motif for Kaiso. After genomic DNAs were bisulphite converted, amplified by PCR using Primer F1 & R1, subcloned, & isolated clones sequenced. 98.6% of CpGs (71 out of 72 colonies) in B6 mice & 76.9% CpGs (50 of 65) in MRL mice were methylated. (E) The structure of Kaiso, SMRT (Silencing Mediator of Retinoic acid & Thyroid hormone receptors)/N-CoR (Nuclear hormone receptor Co-Repressor), & HDAC3 (Histone deacetylase 3) complex. BTB (for BR-C, ttk & bab), POZ (for Pox virus & Zinc finger) & C2H2 zinc-finger domains are shown. (F) DNA sequencing of bisulphite converted DNAs in Ctse intron 1 in B6 & MRL mice. Percentage of methylation of 11th CpG is shown (n = 5). ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30816218), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-152_rabbit-polyclonal-cathepsin-e-antibody-310202416161819.jpg)
![Western Blot: West Nile Virus Envelope Antibody [NB100-56744] - Analysis using the Azide Free version of NB100-56744. Detection of West Nile Virus Envelope in (A) untransfected and (B) transfected cells using this antibody.](http://images.novusbio.com/fullsize/West-Nile-Virus-Envelope-Antibody-Western-Blot-NB100-56744-img0011.jpg "Western Blot: West Nile Virus Envelope Antibody [NB100-56744] - Analysis using the Azide Free version of NB100-56744. Detection of West Nile Virus Envelope in (A) untransfected and (B) transfected cells using this antibody.")
![Immunohistochemistry-Paraffin: West Nile Virus Envelope Antibody [NB100-56744] - Analysis using the Azide Free version of NB100-56744. Staining of West Nile Virus Envelope in bird liver using this antibody at 1 ug/ml, incubated for 30 minutes. A Ventana brand second step antibody conjugated to biotin was applied followed with streptavidin-AKP. The substrate was Fast Red chromagen. The antigen retrieval method used to obtain staining: Ventana Protease 2 for three minutes at room temperature with a Parafilm coverslip. (using an autostainer which keeps residual fluid on the slides during every step, so the final concentration is approximately 2.5 times more dilute). Image and protocol used to generate this image are courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine.](http://images.novusbio.com/fullsize/West-Nile-Virus-Envelope-Antibody-Immunohistochemistry-Paraffin-NB100-56744-img0010.jpg)
![Western Blot: CD98 Antibody (1C11.7E3) [NBP2-36491] - Western blot analysis of human CD98 in recombinant protein, A431, Jurkat, HeLa and HepG2 lysates at 2.0 ug/ml.](http://images.novusbio.com/fullsize/CD98-Antibody-1C11-7E3-Western-Blot-NBP2-36491-img0001.jpg "Western Blot: CD98 Antibody (1C11.7E3) [NBP2-36491] - Western blot analysis of human CD98 in recombinant protein, A431, Jurkat, HeLa and HepG2 lysates at 2.0 ug/ml.")
![Immunohistochemistry-Paraffin: CD98 Antibody (1C11.7E3) [NBP2-36491] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human kidney using CD98 antibody (clone 1C11.7E3) at 1:300 dilution. The antibody generated a distinct/intense membranous staining with relatively weaker cytoplasmic signal, and the immunostaining was exclusively observed in tubular epithelial cells with no signal in the Bowman's capsules.](http://images.novusbio.com/fullsize/CD98-Antibody-1C11-7E3-Immunohistochemistry-Paraffin-NBP2-36491-img0003.jpg)
![Flow (Cell Surface): CD98 Antibody (1C11.7E3) [NBP2-36491] - Flow cytometry analysis of human CD98 in hPBMC. Primary antibody was used at 2.5 ug / million cells. Goat anti-mouse IgG (PE) was used at 0.5 ug / million cells. The red shift line represents the primary antibody, the blue line represents the cells alone and the green line represents the isotype control.](http://images.novusbio.com/fullsize/CD98-Antibody-1C11-7E3-Flow-Cell-Surface-NBP2-36491-img0002.jpg)
![Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Flow-Cytometry-NBP1-60140-img0006.jpg "Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: F4/80 Antibody (BM8) [NBP1-60140] - Red: F4/80, Green: CD31/PECAM-1, Blue: DAPI+ nuclei. Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with PE-conjugated F4/80 and Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) for 2 hours at the room temperature. This image was submitted via a customer review. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Immunohistochemistry-Frozen-NBP1-60140-img0007.jpg)
![Immunohistochemistry: F4/80 Antibody (BM8) [NBP1-60140] - Expression on macrophages in mouse liver. Staining of frozen tissue section with antibody BM8.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Immunohistochemistry-NBP1-60140-img0003.jpg)
![Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Analysis using the FITC conjugate of NBP1-60140. Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 FITC.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Flow-Cytometry-NBP1-60140-img0005.jpg)
![Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 1ug/mL Fc-tagged recombinant human Spike S1 subunit RBD is shown to bind Ace-2 (black dotted line) detected by Human IgG Fc-APC Secondary Antibody (FAB110A). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse Anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0003.jpg "Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 1ug/mL Fc-tagged recombinant human Spike S1 subunit RBD is shown to bind Ace-2 (black dotted line) detected by Human IgG Fc-APC Secondary Antibody (FAB110A). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse Anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).")
![Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 0.5 ug/mL sheep Fc-tagged recombinant human Spike S1 is shown to bind ACE-2 (black dotted line) detected by Donkey anti-Sheep APC Secondary Antibody (Catalog # F0127). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0004.jpg)
![Western Blot: ACE-2 Antibody (AC384) [NBP2-80038] - 1: Human ACE2 Fc-fusion protein. 2: Human ACE2 (Ecto domain) (FLAG®-tagged). 3: HepG2 cell lysate. 4: Other Fc-fusion (hGITR-Fc) proteins (negative control). 1:2'000 dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Western-Blot-NBP2-80038-img0005.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC384) [NBP2-80038] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Mouse Anti-Human ACE-2 (AC384) Monoclonal Antibody (Catalog # NBP2-80038) at 10 ug/ml overnight at 4 degrees C. Detection kit: VC001. Counterstain: Hematoxylin. Localization: respiratory bronchioles.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Immunohistochemistry-Paraffin-NBP2-80038-img0007.jpg)
![Flow Cytometry: ACE-2 Antibody (AC384) [NBP2-80038] - Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # NBP2-80038) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 control antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Flow-Cytometry-NBP2-80038-img0002.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC384) [NBP2-80038] - ACE-2 was detected in immersion fixed paraffin-embedded sections of human lung normal (left) and lung cancer tissue (right) using Mouse Anti-HumanACE-2 (AC18F) Monoclonal Antibody (Catalog # NBP2-80038) at 15ug/mL for 1 hour at room temperature followed by incubation with the Goat anti-Mouse IgG Secondary Antibody.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Immunohistochemistry-Paraffin-NBP2-80038-img0006.jpg)
![Functional: ACE-2 Antibody (AC384) [NBP2-80038] - Binding of ACE2 (human) to the Spike protein of SARS-CoV-2 is inhibited by the antibody anti-ACE2 (human), mAb (blocking) (AC384). Spike (SARS-CoV-2):Fc (human) (RBD) (rec.) is coated on an ELISA plate at 1ug/ml. ACE2 (human), mAb (blocking) (AC384) or an unrelated mAb Control are added (starting at 40 ug/mL with a two-fold serial dilution) together with 500 ng/uL of ACE2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0001.jpg)
![ELISA: Mouse Complement C3a ELISA Kit (Colorimetric) [NBP2-70037] - Samples were spiked with high concentrations of Mouse Complement C3a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70037_mouse-complement-c3a-elisa-kit-colorimetric-13220241626433.jpg "ELISA: Mouse Complement C3a ELISA Kit (Colorimetric) [NBP2-70037] - Samples were spiked with high concentrations of Mouse Complement C3a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Complement C3a ELISA Kit (Colorimetric) [NBP2-70037] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70037_mouse-complement-c3a-elisa-kit-colorimetric-432024941599.jpg)