| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33154 | |||
| IHC-Prediluted | NBP2-44334 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77666SS | |||
| Azide and BSA Free | NBP2-80718 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| Azide and BSA Free | NBP2-80765 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| BSA Free | NBP2-76810 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33136 | |||
| IHC-Prediluted | NBP2-44333 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| Azide and BSA Free | NBP2-80713 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65900-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-229-Immunofluorescence-NB110-58718-img0001.jpg "Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).")
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0006.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Analysis of the APC conjugate of this antibody. A cell surface stain was performed on THP-1 cells with Endoglin antibody NB110-58718APC (blue) along with a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0002.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 antibody (MEM-229) NB110-58718PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0003.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PE.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0004.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) biotin.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0005.jpg)
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Analysis using Azide/BSA FREE version of CD31/PECAM-1 Antibody (C31.3). Formalin-paraffin human Angiosarcoma.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-Immunohistochemistry-Frozen-NBP2-15202-img0013.jpg "Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Analysis using Azide/BSA FREE version of CD31/PECAM-1 Antibody (C31.3). Formalin-paraffin human Angiosarcoma.")
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Analysis using the Azide and BSA Free version of CD31/PECAM-1 Antibody (C31.3). Staining of human tonsil.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-Immunohistochemistry-Paraffin-NBP2-15202-img0012.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.3).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-Western-Blot-NBP2-15202-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with CD31/PECAM-1 Antibody (C31.3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-Immunocytochemistry-Immunofluorescence-NBP2-15202-img0015.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Flow Cytometric analysis of human CD31 on Jurkat cells. Black: cells alone; grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.3).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-Flow-Cytometry-NBP2-15202-img0014.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Staining with CD31/PECAM-1 Antibody (C31.3) [Alexa Fluor® 647] (NBP2-33154AF647) using GE Cell DIVE (20x) on Human Rheumatoid Arthritis synovium (10 ug/ml). Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-15202_mouse-monoclonal-cd31-pecam-1-antibody-c31-3-17720241044649.jpg)
![Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD105 (NB100-77666) in 1% milk/TBST and detected with an anti-rat HRP conjugated secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Western-Blot-NB100-77666-img0001.jpg "Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD105 (NB100-77666) in 1% milk/TBST and detected with an anti-rat HRP conjugated secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Using the DyLight 550 direct conjugate Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and nuclei (blue).](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Immunocytochemistry-Immunofluorescence-NB100-77666-img0004.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Analysis of a FFPE mouse ductus deference using a 1:50 dilution of CD105 antibody (clone MJ7/18) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Staining was observed in the pseudostratified columnar epithelial cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Immunohistochemistry-Paraffin-NB100-77666-img0005.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Analysis of Biotin conjugate of NB100-77666. Surface staining of bEND.3 cell line with 0.25 ug of Anti-Mouse CD105 (Endoglin) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Flow-Cytometry-NB100-77666-img0002.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - A surface stain was performed on MS-1 Cells with Endoglin/CD105 (MJ7/18) antibody NB100-77666 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Flow-Cell-Surface-NB100-77666-img0003.jpg)
![Western Blot: LYVE-1 Antibody Pack [NB100-939] - Total protein from human stomach, lymph node and placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-LYVE1 (NB100-725) in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Pack-Western-Blot-NB100-939-img0017.jpg "Western Blot: LYVE-1 Antibody Pack [NB100-939] - Total protein from human stomach, lymph node and placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-LYVE1 (NB100-725) in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry: LYVE-1 Antibody Pack [NB100-939] - Detection of LYVE-1 in endothelial cells of human lung blood vessels using NB100-725. Note presence of RBCs within vessel lumen.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Pack-Immunohistochemistry-NB100-939-img0004.jpg)
![Western Blot: LYVE-1 Antibody Pack [NB100-939] - Detection of LYVE-1 in mouse spleen lysate using NB 100-726. ECL detection overnight.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Pack-Western-Blot-NB100-939-img0018.jpg)
![Flow Cytometry: LYVE-1 Antibody Pack [NB100-939] - Analysis using the DyLight 488 conjugate of NB100-725. Unlabeled control with labeled samples of cells dissociated mechanically and enzymatically from human skin. Image provided via product review by Patricia Redondo.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Pack-Flow-Cytometry-NB100-939-img0019.jpg)
![Immunohistochemistry: LYVE-1 Antibody Pack [NB100-939] - Detection of LYVE-1 in endothelial cells of human lung blood vessels using NB100-725.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Pack-Immunohistochemistry-NB100-939-img0014.jpg)
![Immunohistochemistry: LYVE-1 Antibody Pack [NB100-939] - Detection of LYVE-1 in endothelial cells of human bladder vasculature using NB100-725.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Pack-Immunohistochemistry-NB100-939-img0002.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Western-Blot-NBP2-75978-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Knockout-Validated-NBP2-75978-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Immunocytochemistry-Immunofluorescence-NBP2-75978-img0002.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (2443C) [NBP2-75978] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte (TM) HRP Polymer Antibody (Catalog # VC002). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Immunohistochemistry-Paraffin-NBP2-75978-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Simple-Western-NBP2-75978-img0007.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75978 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Simple-Western-NBP2-75978-img0008.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with NB500-452F (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0009.jpg "Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with NB500-452F (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to FITC.")
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Surface staining (mass cytometry) of hTERT cell line with anti-human CD105 (MEM-226) 143Nd. Gated on singlets.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0001.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Analysis using the FITC conjugate of NB500-452. Staining of HUVEC (human umbilical vein endothelial cells) with anti-human CD105 (MEM-226) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cell-Surface-NB500-452-img0003.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Surface staining of HUVEC (human umbilical vein endothelial cells) with anti-CD105 (MEM-226) PE. HEK-293 cells used as a negative control.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0004.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cell-Surface-NB500-452-img0005.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452AF647 (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0006.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452PCP (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0007.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Western-Blot-NBP2-80713-img0001.jpg "Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80713-img0002.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80713-img0003.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit Anti- EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80713-img0004.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.7).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-7-Western-Blot-NBP2-15188-img0018.jpg "Western Blot: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.7).")
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Analysis using Azide/BSA FREE version of CD31/PECAM-1 Antibody (C31.7). Formalin-paraffin human Angiosarcoma.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-7-Immunohistochemistry-Paraffin-NBP2-15188-img0012.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Flow Cytometric Analysis of paraformaldehyde-fixed Jurat cells using CD31/PECAM-1 Antibody (C31.7)followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-7-Flow-Cytometry-NBP2-15188-img0017.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Analysis using the Azide and BSA Free version of CD31/PECAM-1 Antibody (C31.7). Detection of CD31 in THP-1 lysate.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-7-Western-Blot-NBP2-15188-img0014.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Flow Cytometric Analysis of human CD31 on Jurkat cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD31/PECAM-1 Antibody (C31.7).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-7-Flow-Cytometry-NBP2-15188-img0015.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Flow Cytometry of human CD31 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.7).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-7-Flow-Cytometry-NBP2-15188-img0016.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunohistochemistry-Paraffin-NBP2-76810-img0003.jpg "Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.")
![Western Blot: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 antibody in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Western-Blot-NBP2-76810-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLight(TM) 557-conjugated Anti-Rabbit IgG secondary antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunocytochemistry-Immunofluorescence-NBP2-76810-img0002.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Flow-Cytometry-NBP2-76810-img0001.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Valvular endothelial cell (VEC) markers. Sections of the sheep aortic valve leaflet stained with antibodies against the endothelial cell markers CD31/PECAM-1, CD34, VE-Cadherin (CD144), or von Willebrand Factor (vWF). Represented are the aortic wall and a section of the valvular leaflet (near the free edge), with](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-CO-3E-1D4-Immunohistochemistry-Paraffin-NB100-65900-img0007.jpg "Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Valvular endothelial cell (VEC) markers. Sections of the sheep aortic valve leaflet stained with antibodies against the endothelial cell markers CD31/PECAM-1, CD34, VE-Cadherin (CD144), or von Willebrand Factor (vWF). Represented are the aortic wall and a section of the valvular leaflet (near the free edge), with")
![Flow Cytometry: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Staining of sheep peripheral blood platelets with Mouse anti Bovine CD31/PECAM-1 Antibody (CO.3E-1D4).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-CO-3E-1D4-Flow-Cytometry-NB100-65900-img0005.jpg)
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Bovine Lung stained with CD31 (green) and DAPI counterstain. Image provided courtesy of Hala Nijmeh, PhD and the PI, Evgenia V. Gerasimovskaya, PhD. Section of Critical Care Medicine, Department of Pediatrics, University of Colorado Anschutz Medical Center.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-CO-3E-1D4-Immunohistochemistry-Frozen-NB100-65900-img0006.jpg)