| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43956 | |||
| BSA Free | NBP2-34488-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56878SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36567SS | |||
| Azide and BSA Free | NBP2-80653 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44740 | |||
| Azide and BSA Free | NBP2-34653-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33088 | |||
| IHC-Prediluted | NBP2-44988 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47672-0.1mg | |||
| IHC-Prediluted | NBP2-48136 | |||
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining in human tonsil and cerebral cortex tissues. Corresponding CD40 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-CL1673-Immunohistochemistry-Paraffin-NBP2-34488-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining in human tonsil and cerebral cortex tissues. Corresponding CD40 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Lane 1: Marker [kDa] Lane 2: Human spleen](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-CL1673-Western-Blot-NBP2-34488-img0014.jpg)
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Human stomach cancer FFPE block stained with CD40 antibody at 1:6000 overnight. Antigen retrieval at pH 9. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-CL1673-Immunohistochemistry-Paraffin-NBP2-34488-img0020.jpg)
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining of human tonsil shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-CL1673-Immunohistochemistry-Paraffin-NBP2-34488-img0016.jpg)
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining of human colon shows moderate membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-CL1673-Immunohistochemistry-Paraffin-NBP2-34488-img0017.jpg)
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining of human cerebral cortex shows no positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-CL1673-Immunohistochemistry-Paraffin-NBP2-34488-img0018.jpg)
![Western Blot: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of CCR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysateLane 2: THP-1 cell lysate](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Western-Blot-NBP2-67700-img0004.jpg "Western Blot: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of CCR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysateLane 2: THP-1 cell lysate")
![Immunohistochemistry-Paraffin: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of paraffin-embedded human esophagus tissue with Rabbit anti-CCR2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Immunohistochemistry-Paraffin-NBP2-67700-img0005.jpg)
![Flow Cytometry: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of K562 cells with CCR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Flow-Cytometry-NBP2-67700-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-CCR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Immunohistochemistry-Paraffin-NBP2-67700-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of paraffin-embedded human tonsil tissue using anti-CCR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Immunohistochemistry-Paraffin-NBP2-67700-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32945-img0002.jpg "Immunocytochemistry/Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded rat spleen dilution: 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0010.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - Sample (30 ug of whole cell lysate) A: BMDM (bone marrow-derived macrophage) cell B: Syk null cell 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0004.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody at a dilution of 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0011.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human cervical cancer diluted at 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0008.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human endometrium diluted at 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0009.jpg)
![Immunoprecipitation: SYK Antibody [NBP1-32945] - Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 ug of SYK antibody. Western blot analysis was performed using SYK antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunoprecipitation-NBP1-32945-img0013.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Syk antibody [N2C2], Internal (NBP1-32945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32945_rabbit-polyclonal-syk-antibody-western-blot-261120251820234.jpg)
![Western Blot: NOD1 Antibody [NB100-56878] - analysis of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using NOD1 antibody at 1:2500.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NB100-56878-img0003.jpg "Western Blot: NOD1 Antibody [NB100-56878] - analysis of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using NOD1 antibody at 1:2500.")
![Immunohistochemistry-Paraffin: NOD1 Antibody [NB100-56878] - analysis of human pancreas using NOD1 antibody at 1:100.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunohistochemistry-Paraffin-NB100-56878-img0002.jpg)
![Western Blot: CD63 Antibody (7G4.2E8) [NBP2-36567] - Western blot analysis of mouse CD63 on recombinant mouse CD63 and mouse kidney lysate.](http://images.novusbio.com/fullsize/CD63-Antibody-7G4-2E8-Western-Blot-NBP2-36567-img0002.jpg "Western Blot: CD63 Antibody (7G4.2E8) [NBP2-36567] - Western blot analysis of mouse CD63 on recombinant mouse CD63 and mouse kidney lysate.")
![Flow Cytometry: CD63 Antibody (7G4.2E8) [NBP2-36567] - Intracellular staining of mouse CD63 in Flow cytometry using 2.0 ug of antibody per 1 million cells. Isotype control was rat IgG2a lambda.](http://images.novusbio.com/fullsize/CD63-Antibody-7G4-2E8-Flow-Cytometry-NBP2-36567-img0003.jpg)
![Western Blot: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Western Blot Analysis of human Raji cell lysate using CD79A Antibody (JCB117 + HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Western-Blot-NBP2-34330-img0005.jpg "Western Blot: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Western Blot Analysis of human Raji cell lysate using CD79A Antibody (JCB117 + HM47/A9).")
![Immunocytochemistry/Immunofluorescence: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Immunofluorescent staining of paraformaldehyde-fixed Raji cells. CD79A Antibody (JCB117 + HM47/A9) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Immunocytochemistry-Immunofluorescence-NBP2-34330-img0004.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD79a MAb (JCB117 + HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Immunohistochemistry-Paraffin-NBP2-34330-img0001.jpg)
![Flow Cytometry: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Flow Cytometric Analysis of Raji cells. CD79A Antibody (JCB117 + HM47/A9) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Flow-Cytometry-NBP2-34330-img0006.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (JCB117 + HM47/A9) [NBP2-34330] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD79a MAb (JCB117 + HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-JCB117-+-HM47-A9-Immunohistochemistry-Paraffin-NBP2-34330-img0002.jpg)
![Western Blot: CD74 Antibody (LN-2) [NBP2-29465] - Western blot analysis of Raji cell lysate using CD74 antibody (LN-2).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Western-Blot-NBP2-29465-img0007.jpg "Western Blot: CD74 Antibody (LN-2) [NBP2-29465] - Western blot analysis of Raji cell lysate using CD74 antibody (LN-2).")
![Immunocytochemistry/Immunofluorescence: CD74 Antibody (LN-2) [NBP2-29465] - Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Immunocytochemistry-Immunofluorescence-NBP2-29465-img0003.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465] - Formalin-fixed, paraffin-embedded human Spleen stained with CD74 Antibody (LN-2).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Immunohistochemistry-Paraffin-NBP2-29465-img0005.jpg)
![Flow Cytometry: CD74 Antibody (LN-2) [NBP2-29465] - Flow Cytometric Analysis of Human Raji cells using CD74 Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Flow-Cytometry-NBP2-29465-img0006.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Antibody (LN-2).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Immunohistochemistry-Paraffin-NBP2-29465-img0004.jpg)
![Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: Human C/EBPbeta. Lane 2: Mouse C/EBPdelta. Lane 3: untransfected. Lane 4: Human C/EBPdelta. 35 ug per lane. Primary: Anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, 35 kDa.](http://images.novusbio.com/fullsize/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0006.jpg "Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: Human C/EBPbeta. Lane 2: Mouse C/EBPdelta. Lane 3: untransfected. Lane 4: Human C/EBPdelta. 35 ug per lane. Primary: Anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, 35 kDa.")
![<b>Genetic Strategies Validation. </b>Western Blot: CEBP Delta Antibody [NB110-85519] - Detection of endogenous protein in whole cell extracts from mouse macrophage cells. Untreated WT cells (Lane 1), WT cells treated 3 hrs with LPS (Lane 2) and knock-out cells treated 3 hrs with LPS (Lane 3). The band at ~35 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of endogenous C/EBP delta. Primary antibody at 1:15,000. ECL was used for detection.](http://images.novusbio.com/fullsize/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: untreated wild-type (WT) cells. Lane 2: WT cells treated 3 hrs with LPS. Lane 3: C/EBPd-knock-out cells treated 3 hrs with LPS. 35 ug per lane. Primary: anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, 35 kDa.](http://images.novusbio.com/fullsize/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0007.jpg)
![Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Western-Blot-NBP2-45316-img0006.jpg "Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).")
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Immunohistochemistry-Paraffin-NBP2-45316-img0002.jpg)
![Flow Cytometry: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Flow Cytometric Analysis of Human Raji cells using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). conjugated with APC (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Flow-Cytometry-NBP2-45316-img0003.jpg)
![Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Human Spleen tissue lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Western-Blot-NBP2-45316-img0005.jpg)
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Histiocytoma stained with HLA-DR Monoclonal Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Immunohistochemistry-Paraffin-NBP2-45316-img0001.jpg)
![Immunofluorescence: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - ICC/IF analysis of Raji cells labeling HLA-DR with conjugated with CF594 (Cyan).The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Immunofluorescence-NBP2-45316-img0004.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-42772-img0011.jpg "Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.")
![Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the Biotin conjugate of NBP1-42772. Detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-42772-img0006.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the HRP conjugate of NBP1-42772. Detection of Lane 1: Recombinant Rat IL-17A. Load: 50 ng per lane. Used at a dilution of 1:1,000 o/n at 4C.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-42772-img0007.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of mouse recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-42772-img0010.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain.TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0023.jpg "Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain.TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - 59T xenograft, using TRAF6 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0012.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Mouse lung. TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0020.jpg)
![Immunoprecipitation: TRAF-6 Antibody [NBP1-33357] - IP samples: K562 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of TRAF6 protein by 4 ug TRAF6 antibody [N2C2], Internal 7.5 % SDS-PAGE. The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunoprecipitation-NBP1-33357-img0016.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody [N2C2], Internal (NBP1-33357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33357_rabbit-polyclonal-traf-6-antibody-western-blot-26112025193619.jpg)
![TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal (NBP1-33357) diluted at 1:100.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-33357_rabbit-polyclonal-traf-6-antibody-immunocytochemistry-immunofluorescence-2611202518503015.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser276] Antibody-Western Blot-NBP1-77807-img0010.jpg "Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.")
![Immunohistochemistry: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - Diluted to1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-NBP1-77807-img0007.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS529 shows phospho p65 staining in carcinoma cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0005.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS276 shows phospho p65 staining in carcinoma cells. Western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes and does not show significant levels of phosphorylated p65. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0006.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0008.jpg)
