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![Western Blot: BDNF Antibody [NB100-98682] - BDNF lowered the mechanical withdrawal threshold further and promoted activation of astrocytes and microglia, and enhanced the p38/JNK pathway to aggravate the release of IL-1beta and TNF-alpha in the SDH of CYP-induced cystitis. Western blots showing the expression of BDNF was further upregulated when compared with the CYP + PBS group. Data of mechanical withdrawal threshold were analyzed using a two-way analysis of variance (ANOVA) followed by the Sidak's multiple comparisons test. All data were calculated as mean +/- SEM (n = 10 per group). **p < 0.01, ***p < 0.001 vs. the control group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the CYP + rBDNF group. $p < 0.05, $$p < 0.01, $$$p < 0.001 vs. the CYP + PBS group.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NB100-98682-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: BDNF Antibody [NB100-98682] - BDNF lowered the mechanical withdrawal threshold further and promoted activation of astrocytes and microglia, and enhanced the p38/JNK pathway to aggravate the release of IL-1beta and TNF-alpha in the SDH of CYP-induced cystitis. Western blots showing the expression of BDNF was further upregulated when compared with the CYP + PBS group. Data of mechanical withdrawal threshold were analyzed using a two-way analysis of variance (ANOVA) followed by the Sidak's multiple comparisons test. All data were calculated as mean +/- SEM (n = 10 per group). **p < 0.01, ***p < 0.001 vs. the control group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the CYP + rBDNF group. $p < 0.05, $$p < 0.01, $$$p < 0.001 vs. the CYP + PBS group.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BDNF Antibody [NB100-98682] - Effects of mBMSC and EA treatment on the expression of mBDNF and NT4 in neurons. Photomicrographs for mBDNF/NeuN or NT4/NeuN double-positive cells in the striatum and hippocampus (n = 5). The number of mBDNF/NeuN double-positive cells in the SVZ + striatum and hippocampus was significantly higher in the EA-treated MCAO group and the combined mBMSC + EA-treated MCAO group, compared to the number in the other groups. The number of NT4/NeuN double-positive cells in the hippocampus was significantly higher in the EA-treated MCAO group than in the vehicle-treated MCAO group. #p < 0.05 and ##p < 0.01 versus control group; *p < 0.05, **p < 0.01, and ***p < 0.001 versus vehicle-treated MCAO group; &&p < 0.01 versus mBMSC-treated MCAO group. Scale bar = 100 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-018-20481-3) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-NB100-98682-img0007.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - Human platelet lysate and recombinant mature BDNF. Blocking: 1% LFDM for 30 min at RT; primary antibody (1:1000) incubated at 4C overnight.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NB100-98682-img0006.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NB100-98682-img0001.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NB100-98682-img0003.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NB100-98682-img0005.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - Effect of treatment with FX extract on CREB/BDNF signaling. (a-c) Differences in the phosphorylation of CREB & expression of BDNF between groups in the PFC & hippocampus (n = 3-4). (d, e) pCREB & BDNF immunofluorescence was assessed in the PFC & hippocampus (n = 3-4). Mean ± SD. #P < 0.05 versus normal group; ∗P < 0.05 & ∗∗P < 0.01 versus control group. Scale bar = 200 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30065945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-98682_rabbit-polyclonal-bdnf-antibody-310202415371951.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - Effect of treatment with FX extract on CREB/BDNF signaling. (a-c) Differences in the phosphorylation of CREB & expression of BDNF between groups in the PFC & hippocampus (n = 3-4). (d, e) pCREB & BDNF immunofluorescence was assessed in the PFC & hippocampus (n = 3-4). Mean ± SD. #P < 0.05 versus normal group; ∗P < 0.05 & ∗∗P < 0.01 versus control group. Scale bar = 200 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30065945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-98682_rabbit-polyclonal-bdnf-antibody-310202415175223.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - Antagonized TrkB could restrain the activation of astrocytes & microglia & supress the p38/JNK pathway to alleviate the release of IL-1 beta & TNF-alpha in the SDH of CYP-induced cystitis. Western blots showed that the overexpression of a BDNF, b TrkB, cp-TrkB, d Iba1, e GFAP, fp-p38, gp-JNK, h TNF-alpha , & i IL-1 beta were downregulated in comparison to the CYP + DMSO group after ANA-12 treatment. All data were calculated as mean ± SEM (n = 5 per group). **p < 0.01, ***p < 0.001 vs. control group. ##p < 0.01, ###p < 0.001 vs. CYP + ANA-12 group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31931832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-98682_rabbit-polyclonal-bdnf-antibody-310202415291934.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - BDNF lowered the mechanical withdrawal threshold further & promoted activation of astrocytes & microglia, & enhanced the p38/JNK pathway to aggravate the release of IL-1 beta & TNF-alpha in the SDH of CYP-induced cystitis. a BDNF treated every other day after CYP injection could further lower the mechanical withdrawal threshold & suppress the retrieval of mechanical threshold when compared with the CYP + PBS group. After the exogenous BDNF injection, Western blots showing the expression of b BDNF, c TrkB, dp-TrkB, e Iba1, f GFAP, gp-p38, hp-JNK, i TNF-alpha , & j IL-1 beta were all further upregulated when compared with the CYP + PBS group. Data of mechanical withdrawal threshold were analyzed using a two-way analysis of variance (ANOVA) followed by the Sidak's multiple comparisons test. All data were calculated as mean ± SEM (n = 10 per group). **p < 0.01, ***p < 0.001 vs. the control group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the CYP + rBDNF group. $p < 0.05, $$p < 0.01, $$$p < 0.001 vs. the CYP + PBS group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31931832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-98682_rabbit-polyclonal-bdnf-antibody-310202416284921.jpg)
![Immunohistochemistry: BDNF Antibody [NB100-98682] - Effects of mBMSC & EA treatment on the expression of trophic factors. (A) Photomicrographs & (B‒D) histograms for mBDNF, NT4, & VEGF expression (n = 4–5). mBDNF expression was markedly increased by treatment with mBMSC and/or EA compared to vehicle treatment. mBDNF expression levels were higher in the EA-treated MCAO group than in the mBMSC-treated MCAO group. NT4 expression in the SVZ & hippocampus was significantly higher in the combined mBMSC & EA treatment group than in the other groups. VEGF expression in the hippocampus was markedly higher in the mBMSC treatment group than in the vehicle-treated MCAO group. #p < 0.05, ##p < 0.01, & ###p < 0.001 versus control group; *p < 0.05, **p < 0.01, & ***p < 0.001 versus vehicle-treated MCAO group; &p < 0.05, &&p < 0.01, & &&&p < 0.001 versus mBMSC-treated MCAO group; Scale bar = 1 mm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29391466), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-98682_rabbit-polyclonal-bdnf-antibody-310202416284912.jpg)
![Western Blot: BDNF Antibody [NB100-98682] - The expression of BDNF-TrkB signaling in the SDH of CYP-induced cystitis. a Changes of the mechanical threshold in CYP-induced cystitis model. Compared to that in the control group, the mechanical threshold of the cystitis group decreased significantly after the CYP injection & remained low until day 17, & the minimum threshold value was reached on day 12. The expression of b BDNF, c TrkB, & dp-TrkB were evaluated by western blots. Compared to the control group, they were upregulated on days 8, 12, & 17. e Immunofluorescence double staining assay of BDNF & p-TrkB in the SDH. BDNF & p-TrkB (red), NeuN, GFAP, & OX-42( green), co-localization (yellow). BDNF was mainly colocalized in neurons which mainly located in Laminate II to IV. & TrkB receptors expressed in neurons, microglia, & astrocytes. The white dotted lines in picture “BDNF/NeuN” showed the laminate of the SDH according to Rexed & Steiner. Scale bar = 100 μm. All data were calculated as mean ± SEM (n = 5 per group). *p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31931832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-98682_rabbit-polyclonal-bdnf-antibody-310202416284910.jpg)
![Western Blot: BDNF Antibody [NBP1-46750] - Human platelet lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody incubated at 4C overnight. Click on eLAB BOOK tab to see the details. Mature BDNF: (1:2000 dilution) ; proBDNF: (1:1000 dilution)](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-46750-img0002.jpg "Western Blot: BDNF Antibody [NBP1-46750] - Human platelet lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody incubated at 4C overnight. Click on eLAB BOOK tab to see the details. Mature BDNF: (1:2000 dilution) ; proBDNF: (1:1000 dilution)")
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - IHC-P on paraffin sections of rat hippocampus.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0008.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat spinal cord. Dilution 1: 1000.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0003.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat hippocampus. Dilution 1: 1000.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0004.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of mouse hippocampus. Dilution 1: 1000.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0005.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - IHC-P on paraffin sections of rat hippocampus.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0007.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - ANT-DBS inhibited BDNF-TrkB pathway and its downstream regulator in the hippocampus of epileptic monkeys. Analysis of BDNF (1:1000, NBP1-59304), TrkB (1:500, NPB1-47898), p-ERK, ERK, p-Akt, Akt, p-S6K and S6K by western blotting. Image collected and cropped by CiteAb from the following publication (www.aging-us.com/lookup/doi/10.18632/aging.103026) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0009.jpg "Western Blot: BDNF Antibody [NBP1-59304] - ANT-DBS inhibited BDNF-TrkB pathway and its downstream regulator in the hippocampus of epileptic monkeys. Analysis of BDNF (1:1000, NBP1-59304), TrkB (1:500, NPB1-47898), p-ERK, ERK, p-Akt, Akt, p-S6K and S6K by western blotting. Image collected and cropped by CiteAb from the following publication (www.aging-us.com/lookup/doi/10.18632/aging.103026) licensed under a CC-BY license.")
![Immunohistochemistry: BDNF Antibody [NBP1-59304] - Ventral horn region of mouse spinal cord. Concentration 1:200](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-NBP1-59304-img0004.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - ACHN Whole Cell lysates.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0005.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - Human Liver cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0002.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - ACHN Whole Cell lysates, Antibody Dilution: 1 ug/ml.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0006.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - Fetal Liver lysates, Antibody Dilution: 0.5 ug/ml.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0007.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - Sample Tissue: Human ACHN Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0008.jpg)
![Immunohistochemistry: BDNF Antibody [NBP1-59304] - Rhesus macaque spinal cord. Concentration1:300.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-NBP1-59304-img0003.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - Simvastatin treatment increases BDNF expression in primary cortical neurons of AS mice. (A) Primary cultured cortical neurons prepared from wild type & AS embryos were treated with 5 μM simvastatin at DIV14 for 12 h. Neurons were then fixed & processed for double immunofluorescence staining using Ube3a & BDNF antibodies. Representative images were shown. About 12 immunostained neurons in each group were checked for BDNF expression, fluorescence intensity in each cell was quantified & compared. Scale bar, 50 μm. (B) Immunoblot analysis of matured BDNF levels in simvastatin treated primary cortical neurons as described above. (C) Band intensity of the mature BDNF was quantified & normalized to Tuj1 (BDNF/Tuj1). Values are mean ± SD; n = 3. The ‘a’ point P < 0.05 compared to vehicle treated wild type group & “b” denote P < 0.05 with respect to vehicle treated AS group (one way ANOVA with Holm–Sidak post hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31849603), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-59304_rabbit-polyclonal-bdnf-antibody-310202416182644.jpg)
![Western Blot: Syntenin 1 Antibody [NBP1-33661] - HeLa whole cell extracts and HeLa exosome extract (3.5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Western-Blot-NBP1-33661-img0011.jpg "Western Blot: Syntenin 1 Antibody [NBP1-33661] - HeLa whole cell extracts and HeLa exosome extract (3.5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody [NBP1-33661] - Analysis of methanol-fixed HeLa, using Syntenin 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33661-img0002.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Mouse kidney. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33661-img0005.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Mouse kidney. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33661-img0006.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Rat fore brain. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33661-img0007.jpg)
![Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein by immunohistochemical analysis. Samples: Frozen Sectioned adult mouse brain.Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term (NBP1-33661) diluted at 1:250.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/nbp1-33661_rabbit-polyclonal-syntenin-1-antibody-immunohistochemistry-frozen-261120251818924.jpg)
![Zebrafish tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [C2C3], C-term (NBP1-33661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33661_rabbit-polyclonal-syntenin-1-antibody-western-blot-261120251836599.jpg)
![Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein by immunohistochemical analysis. Samples: Frozen Sectioned adult mouse brain.Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term (NBP1-33661) diluted at 1:250.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/nbp1-33661_rabbit-polyclonal-syntenin-1-antibody-immunohistochemistry-frozen-26112025182708.jpg)
![Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - WB on brain lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:5000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Western-Blot-NBP1-20180-img0006.jpg "Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - WB on brain lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:5000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen.Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0022.jpg)
![Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - Rat brain lysate using Rabbit antibody to P2RX7 at 1:500 dilution. Incubated 30 min at RT with shake. Blocking: 0.5% LFDM in 1x PBS containing 0.1% Tween-20](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Western-Blot-NBP1-20180-img0005.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0010.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0011.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0012.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0013.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0014.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0015.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0016.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0017.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0018.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0019.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0020.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (35928.11) [NB120-10505] - Human thyroid tissue was incubated with anti-BDNF antibody at a dilution of 1:100 overnight at 4C and detected with Alexa Fluor 594 Donkey anti-mouse 1:500. Nuclei stained with DAPI. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/BDNF-Antibody-35928-11-Immunocytochemistry-Immunofluorescence-NB120-10505-img0001.jpg "Immunocytochemistry/Immunofluorescence: BDNF Antibody (35928.11) [NB120-10505] - Human thyroid tissue was incubated with anti-BDNF antibody at a dilution of 1:100 overnight at 4C and detected with Alexa Fluor 594 Donkey anti-mouse 1:500. Nuclei stained with DAPI. ICC/IF image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46629 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46629 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Western-Blot-NBP2-46629-img0009.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse midbrain shows moderate positivity in GABergic neural fibers in the substantia nigra.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0014.jpg)
![Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Western-Blot-NBP2-46629-img0008.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of rat basal forebrain shows moderate immunoreactivity in GABAergic fibers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0001.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse substantia nigra shows moderate positivity in GABAergic fibers in pars reticulata.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0002.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of rat globus pallidus shows moderate positivity in GABAergic neural fibers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0004.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human cerebellum shows moderate immunoreactivity in GABAergic fibers in granular and molecular layers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0005.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human cerebral cortex shows moderate to strong positivity in GABAergic neuronal processes.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0011.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human pancreas shows moderate cytoplasmic positivity in endocrine glandular cells.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0012.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0013.jpg)
![Western Blot: P2X7/P2RX7 Antibody [NBP1-37775] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Western-Blot-NBP1-37775-img0005.jpg "Western Blot: P2X7/P2RX7 Antibody [NBP1-37775] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-37775] - Staining of paraffin embedded Human Kidney. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-37775-img0007.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-37775] - Staining of paraffin embedded Human Brain Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-37775-img0006.jpg)
![Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of BDNF on different lysates using anti-BDNF antibody at 1/1,000 dilution. Positive control: Lane 1: SHG-44 Lane 2: A172 Lane 3: Mouse brain](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Western-Blot-NBP2-67410-img0005.jpg "Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of BDNF on different lysates using anti-BDNF antibody at 1/1,000 dilution. Positive control: Lane 1: SHG-44 Lane 2: A172 Lane 3: Mouse brain")
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (SJ12-09) [NBP2-67410] - ICC staining of BDNF in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary ant](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunocytochemistry-Immunofluorescence-NBP2-67410-img0006.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse testis tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0004.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded human lung tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0001.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse brain tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0002.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse heart tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0003.jpg)
![Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Western-Blot-NBP1-33284-img0012.jpg "Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-33284] - RT2 xenograft. GAD65 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-Paraffin-NBP1-33284-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - Paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2500.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33284-img0008.jpg)
![GAD65 antibody detects GAD65 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAD65 stained by GAD65 antibody (NBP1-33284) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33284_rabbit-polyclonal-gad2-gad65-antibody-immunocytochemistry-immunofluorescence-2611202518365910.jpg)
![Immunocytochemistry/Immunofluorescence: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP1-19788] - Antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/beta-1-3-Glucuronyltransferase-1-B3GAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19788-img0005.jpg "Immunocytochemistry/Immunofluorescence: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP1-19788] - Antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP1-19788] - Analysis in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/beta-1-3-Glucuronyltransferase-1-B3GAT1-Antibody-Immunohistochemistry-NBP1-19788-img0006.jpg)