| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92687SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-147SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22122SS | |||
| Azide and BSA Free | NBP2-80719 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27293SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30440SS | |||
| Azide and BSA Free | NBP2-80715 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80671 | |||
| BSA Free | NB100-136SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87102-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-2525SS | |||
![Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Knockdown-Validated-NBP1-92687-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) [NBP1-92687] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NBP1-92687-img0003.jpg)
![Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Analysis of different cell lysates using mouse mAb to vimentin, NBP1-92687, dilution 1:10,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, and [6] C6 cells. The band at about 50kDa mark corresponds to the vimentin protein.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Western-Blot-NBP1-92687-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) [NBP1-92687] - Analysis of cortical neuron-glial cell cultures from E20 rat stained with mouse mAb to vimentin, NBP1-92687, dilution 1:2,000 in red, and costained with chicken pAb to glial fibrillary acidic protein (GFAP), dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Fibroblastic and other developing cells express only vimentin and appear red. Astrocytes that express GFAP only are green while those that express both GFAP and vimentin appear golden yellow.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NBP1-92687-img0010.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (2D1) [NBP1-92687] - Human breast cancer tissue stained for Vimentin (white) and countersatined with DAPI (blue). Alexa Fluor 750 version of antibody used (NBP1-92687AF750). Image from verified customer review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Immunohistochemistry-Paraffin-NBP1-92687-img0012.jpg)
![Immunohistochemistry: Vimentin Antibody (2D1) [NBP1-92687] - Mouse cortex microslice section (from postnatal day 3) was immunostained with (1:500 dil.). This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Immunohistochemistry-NBP1-92687-img0005.jpg)
![Simple Western: Vimentin Antibody (2D1) [NBP1-92687] - Simple Western lane view shows a specific band for Vimentin in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Simple-Western-NBP1-92687-img0004.jpg)
![Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - High glucose (HG) concentrations induced epithelial-to-mesenchymal transition protein expression & enhanced migration activity in colorectal cancer (CRC) cells. SW480 (low metastatic potential) & SW620 (high metastatic potential) cells were cultured in different concentrations of glucose (normal: NG; HG; & osmotic control: NG + l-glucose). (A) Morphological change occurred from epithelial to mesenchymal type in the HG-concentration group. (B) HG concentration caused downregulation of E-cadherin & upregulation of N-cadherin, beta CTN, & vimentin, but c-myc was unchanged, as detected using Western blotting. beta -actin was evaluated as an internal control. (C,D) Wound healing assay showed that HG concentration promoted cell motility in SW480 & SW620 CRC cells after 48 & 72 h of culture, compared with the NG & NG + l-glucose groups. (E) In a Transwell migration assay, 3.5 × 105 SW480 & SW620 CRC cells were plated onto a 24-well plate & cultured in NG & HG-concentration medium for 96 h. HG concentration promoted cell motility in SW480 & SW620 cells. NG + l-glucose cells were evaluated as ostomic controls. (F) These data show that HG concentration caused upregulation of p-IGF1R in CRC. In addition, HG concentration promoted IGF1R downstream signaling, including p-Src & p-ERK; these proteins were increased when CRC cells were cultured in HG-concentration medium. Levels of beta -actin were evaluated as loading controls. Statistically significant differences between the two groups were judged using Student’s t-tests; * p < 0.05, ** p < 0.005, *** p < 0.001; n.s. = nonsignificant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30965609), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-92687_mouse-monoclonal-vimentin-antibody-2d1-310202416113533.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Western-Blot-NB300-147-img0005.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.")
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Sodium Potassium ATPase Beta 1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Immunocytochemistry-Immunofluorescence-NB300-147-img0008.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Detection of Na, K-ATPase (beta) in rat kidney homogenates (20 ug)using NB 300-147. Lane 1: 1:2,500 primary. Lane 2: 1:5,000 primary.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Western-Blot-NB300-147-img0007.jpg)
![Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Western-Blot-NBP2-22122-img0006.jpg "Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Human endometrial stromal cells stained with Endoglin/CD105 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Immunocytochemistry-Immunofluorescence-NBP2-22122-img0014.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Immunohistochemical staining of endoglin (CD105) in B16F10 murine melanoma tumor model. IHC-P image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Immunohistochemistry-Paraffin-NBP2-22122-img0013.jpg)
![Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysates.](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-Western-Blot-NBP2-22122-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of HepG2 cells using CD105 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red).](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-Immunocytochemistry-Immunofluorescence-NBP2-22122-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - HMSC cells stained with anti-CD105 antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Immunocytochemistry-NBP2-22122-img0007.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-Immunohistochemistry-Paraffin-NBP2-22122-img0005.jpg)
![ELISA: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Red: Control Antigen (100 ng), Purple: Antigen (10 ng), Green: Antigen (50 ng), Blue: Antigen (100 ng).](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-ELISA-NBP2-22122-img0001.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Liver tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.](http://images.novusbio.com/fullsize/iNOS-Antibody-K13-A-Immunohistochemistry-Paraffin-NBP1-33780-img0011.jpg "Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Liver tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.")
![Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Lung adenocarcinoma tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.](http://images.novusbio.com/fullsize/iNOS-Antibody-K13-A-Immunohistochemistry-Paraffin-NBP1-33780-img0012.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Inflamed porcine intestine. Antibody dilution 1:200, overnight 4C. Secondary anti-rabbit Alexa 488 (1:1000) 2 hr incubation. Image from verified customer review.](http://images.novusbio.com/fullsize/iNOS-Antibody-K13-A-Immunohistochemistry-Paraffin-NBP1-33780-img0010.jpg)
![Western Blot: iNOS Antibody (K13-A) [NBP1-33780] - Effects of omega-3 supplementation on the immunocontent of macrophages M1 markers: nitric oxide synthase 2 (NOS-2) (A) & CD86 (B), & M2 marker: Arginase-1 (C) on the mouse paw muscle 48 h after the model induction in Saline/Sham, Saline/CPIP, Omega-3/CPIP, & Corn oil/CPIP groups. Data are expressed as mean ± SD of 6 animals per group, statistically assessed by the one-way ANOVA followed by Tukey’s test. ##p < 0.01 vs. the Saline/Sham group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35391753), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33780_rabbit-monoclonal-inos-antibody-k13-a-31020241535639.jpg)
![Immunohistochemistry-Paraffin: Snail Antibody [NBP2-27293] - Analysis of a FFPE tissue section of mouse placenta using 1:200 dilution of SNAIL antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Snail-Antibody-Immunohistochemistry-Paraffin-NBP2-27293-img0007.jpg)
![Immunohistochemistry-Paraffin: Snail Antibody [NBP2-27293] - Analysis of in FFPE human kidney tissue using this antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Snail-Antibody-Immunohistochemistry-Paraffin-NBP2-27293-img0004.jpg)
![Simple Western: Snail Antibody [NBP2-27293] - Image shows a specific band for SNAIL in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Snail-Antibody-Simple-Western-NBP2-27293-img0002.jpg)
![Western Blot: Snail Antibody - BSA Free [NBP2-27293] - SiRNAs against HER2 could abrogate the EMT phenotype & cell invasiveness in cisplatin-resistant gastric cancer cells.(A) Validation of silencing efficiency of HER2. Cells were transiently transfected with 50 nM HER2-specific siRNAs #1-#3 or a control siRNA for 48 h. (B) Knock-down of HER2 abrogated EMT morphology. Cells were transiently transfected with 50 nM specific siRNAs targeting HER2 for 48 h, Representative images of cellular morphology were shown. (C) Knock-down of HER2 abolished EMT phenotype. Cells were treated as in Fig. 4B. FITC-phalloidin staining of F-actin were performed & representative images were shown. (D) Effects of HER2 knock-down on the levels of ZO1 & Snail. (E) Effects of HER2 knock-down on cell migration. Representative images of transwell cell migration assay were shown. (F) Summary of Fig. 4E. Values represented the mean ± SD from three independent experiments with triplicate samples. *P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26846307), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27293_rabbit-polyclonal-snail-antibody-310202416114759.jpg)
![Western Blot: Snail Antibody - BSA Free [NBP2-27293] - HER2 is overexpressed & both Herceptin incubation & siRNAs against HER2 reversed the EMT in cisplatin-resistant gastric cancer cells.(A) Western blot of HER2 in MGC803/DDP & AGS/DDP cell lines, & parental MGC803 & AGS cells. GAPDH was used as loading control. (B) Relative amplification of HER2 in parental & cisplatin-resistant cells. Results were presented as the mean ± SD of two independent experiments, *P < 0.05 compared with parental cells. (C) Herceptin incubation abrogated EMT morphology in MGC803/DDP cells. Representative images of cellular morphology of MGC803/DDP cells, plus parental MGC803 cells treated with Herceptin for 24 h were shown. Hercep represents Herceptin. (D) Herceptin incubation reversed EMT morphology in MGC803/DDP cells. Representative images of FITC-phalloidin staining of F-actin after 100 μg/ml Herceptin treatment for 24 h were shown. (E) Effects of Herceptin on levels of ZO1 & Snail. (F) CP724714 treatment abolished EMT-like cell morphology. Cells were incubated with 10 μM CP724714 for 24 h. Representative images of cellular morphology of were captured. (G) CP724714 treatment abrogated EMT-like cell morphology. Cells were treated as in Fig. 4F, FITC-phalloidin staining of F-actin was performed & typical images were shown. (H) Effects of CP724714 on ZO1 & Snail. (I) CP724714 inhibited the increased cell migration in cisplatin-resistant gastric cancer cells. Representative images were presented here. (J) CP724714 inhibited the increased cell migration in cisplatin-resistant gastric cancer cells. Values represented the mean ± SD from three independent experiments with triplicate samples. *P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26846307), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27293_rabbit-polyclonal-snail-antibody-310202416113529.jpg)
![Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Analysis of HIF-2 alpha in human endometrium using. Donkey anti-mouse Alexa Fluor 488 secondary antibody was used. Image from verified customer review. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0010.jpg "Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Analysis of HIF-2 alpha in human endometrium using. Donkey anti-mouse Alexa Fluor 488 secondary antibody was used. Image from verified customer review. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]")
![<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - WNT11 is induced by hypoxia or hypoxic mimetics in different cell types. Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody [NB100-122]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Western-Blot-NB100-902HIF2-img0012.jpg)
![Simple Western: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Lane view shows a specific band for HIF-2 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Simple-Western-NB100-902HIF2-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - HIF-1alpha is the predominant transcriptional regulator of WNT11 expression during hypoxia. EMSCs isolated from the indicated mouse genotypes were infected with lentivirus expressing GFP or Cre recombinase. Non-infected cells and GFP infected cells served as controls. Immunoblot analyses of EMSCs derived from the indicated genotypes treated with 0.1 mM DMOG for 24 hrs. Attenuated WNT11 expression in Hif-1alpha KO EMSCs (lenti-Cre infected Hif-1af/f). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody [NB100-122]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Knockout-Validated-NB100-902HIF2-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Formalin-fixed paraffin-embedded tissue sections of human placenta were probed for HIF-2 alpha/EPAS1 mRNA (ACD RNAScope Probe, catalog #410598; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog # NB100-122) at 1:100 dilution with one-hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to trophoblastic cells. HIF-2 alpha/EPAS1 Antibody [NB100-122]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0014.jpg)
![Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Normal ganglia 1 (NG1), normal ganglia 2 (NG2) and IDH-mutant (IDH). Sporadic PGL (Spo. PGL). HIF2 alpha staining. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0127471) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0011.jpg)
![Western Blot: EGLN3/PHD3 Antibody (EG188e/d5) [NBP1-30440] - PHD3 expression in SK-N-BE(2) whole cell lysates. Cells were treated in normoxia (left) and in hypoxia (right). Image from verified customer review.](http://images.novusbio.com/fullsize/EGLN3-PHD3-Antibody-EG188e-d5-Western-Blot-NBP1-30440-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGLN3/PHD3 Antibody (EG188e/d5) [NBP1-30440] - PHD3 expression in SK-N-BE(2) whole cell lysates. Cells were treated in normoxia (left) and in hypoxia (right). Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: EGLN3/PHD3 Antibody (EG188e/d5) [NBP1-30440] - Analysis of HIF Prolyl Hydroxylase 3 in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/EGLN3-PHD3-Antibody-EG188e-d5-Immunohistochemistry-NBP1-30440-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: EGLN3/PHD3 Antibody (EG188e/d5) [NBP1-30440] - Analysis of HIF Prolyl Hydroxylase 3 in (1) Cobalt chloride treated COS-7 nuclear extracts and (2) Untreated COS-7 nuclear extracts.](http://images.novusbio.com/fullsize/EGLN3-PHD3-Antibody-EG188e-d5-Western-Blot-NBP1-30440-img0004.jpg)
![Simple Western: EGLN3/PHD3 Antibody (EG188e/d5) [NBP1-30440] - Lane view shows a specific band for PHD3/HIF Prolyl Hydroxylase 3 in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGLN3-PHD3-Antibody-EG188e-d5-Simple-Western-NBP1-30440-img0008.jpg)
![Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Western-Blot-NBP1-20136-img0002.jpg "Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0003.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0004.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0005.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0006.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0007.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0008.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0009.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0010.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0011.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0012.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0013.jpg)
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0014.jpg)
![Western Blot: Cited-2 Antibody (JA22) [NB100-136] - Total protein from human HeLa and MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cited in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Cited-2-Antibody-JA22-Western-Blot-NB100-136-img0005.jpg "Western Blot: Cited-2 Antibody (JA22) [NB100-136] - Total protein from human HeLa and MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cited in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunocytochemistry/Immunofluorescence: Cited-2 Antibody (JA22) [NB100-136] - The Cited-2 (JA22) antibody was tested at a 1:250 dilution in HeLa cells against Dylight 488 (Green). Beta-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/Cited-2-Antibody-JA22-Immunocytochemistry-Immunofluorescence-NB100-136-img0003.jpg)
![Immunohistochemistry-Paraffin: Cited-2 Antibody (JA22) [NB100-136] - IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of human breast cancer using Cited-2 antibody at 1:500 dilution. The primary antibody bound to CAIX antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Staining was localized to the nucleus.](http://images.novusbio.com/fullsize/Cited-2-Antibody-JA22-Immunohistochemistry-Paraffin-NB100-136-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Analysis in human cerebral cortex and liver tissues. Corresponding S100B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Analysis in human cerebral cortex and liver tissues. Corresponding S100B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: S100B Antibody [NBP1-87102] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-Western-Blot-NBP1-87102-img0010.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0025.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in purkinje cell layer.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0022.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0023.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0024.jpg)
![Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in 293 cell lysates.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Western-Blot-NB100-2525-img0007.jpg "Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in 293 cell lysates.")
![Immunocytochemistry/Immunofluorescence: ERO1L Antibody [NB100-2525] - Detection of ERO1L in Hela cells.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2525-img0006.jpg)
![Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in mouse white adipose tissue using anti-ERO1L antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Western-Blot-NB100-2525-img0008.jpg)
![Western Blot: ERO1L Antibody [NB100-2525] - Detection of ERO1 alpha in an endogenous 293T cell lysate.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Western-Blot-NB100-2525-img0005.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-39681] - Analysis of anti-nNOS / NOS1 antibody with human brain, cerebellum at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunohistochemistry-Paraffin-NBP1-39681-img0004.jpg)
![Western Blot: nNOS Antibody [NBP1-39681]](http://images.novusbio.com/fullsize/nNOS-Antibody-Western-Blot-NBP1-39681-img0006.jpg)
![Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-39681] - Analysis of anti-nNOS / NOS1 antibody with human brain, cortex at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunohistochemistry-Paraffin-NBP1-39681-img0003.jpg)
![Western Blot: nNOS Antibody [NBP1-39681] - Laser-Catapult Microdissection of Immunolabeled ventromedial hypothalamic nucleus (VMN) GABA or Nitrergic Neurons: Western Blot Confirmation of Accuracy of Immunocytochemical Identification of Neurotransmitter Phenotype. VMN neurons were identified in situ for glutamate decarboxylase65/67 (GAD65/67)- (top row, Panel 1A) or neuronal nitric oxide (nNOS)-immunoreactivity (-ir) (bottom row, Panel 2A); representative GAD65/67- or nNOS-ir-positive neurons are indicated by blue arrows. Areas shown in Panels 1A & 2A were rephotographed after positioning of a continuous laser track (depicted in green) around a single GAD65/67-ir (Panel 1B, blue arrow) or nNOS-ir neuron (Panel 2B, blue arrow) & subsequent ejection of the encircled cell by laser pulse (Panels 1C & 2C). Note that this microdissection technique causes negligible destruction of surrounding tissue & minimal inclusion of adjacent tissue. Western blot analysis of triplicate cell lysate pools produced from immunolabeled GAD65/67 (Panel 1D) or nNOS (Panel 2D) neurons from Veh/Veh animals showed that these proteins are expressed in VMN neuron samples obtained by combinatory immunocytochemistry/laser-catapult microdissection. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-39681_rabbit-nnos-pab-310202416154636.jpg)
![Western Blot: nNOS Antibody [NBP1-39681] - Effects of ER alpha & ER beta Antagonists on NE Regulation of VMN GAD & nNOS Protein Expression. Micropunch-dissected VMN tissue was obtained from groups of ovariectomized, estradiol-replaced female rats pretreated by intra-VMN delivery of MPP, PHTPP, or vehicle (Veh) prior to NE administration for Western blot analysis of neuronal nitric oxide synthase (nNOS)—Panel 2A, F(5, 12) = 12.67, p < .0001—or glutamate decarboxylase65/67 (GAD)—Panel 2B, F(5, 12) = 7.13, p = .0003—protein expression. Data depict mean normalized protein optical density (O.D.) values ± SEM for groups of rats (n = 6 per group) infused with Veh (white bars) or NE (gray bars) after delivery of Veh (solid bars), MPP (diagonal-striped bars), or PHTPP (cross-hatched bars) administration. *p < .05; **p < .01; ***p < .001. MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-39681_rabbit-nnos-pab-310202416175279.jpg)