| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70146 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37736-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02974-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95252-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB600-862-0.1mg | |||
| HRP | NB600-863-0.1mg | |||
![Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Western-Blot-NBP2-45602-img0008.jpg "Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A.")
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HepG2 cells.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0003.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Carcinoma of Human liver tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0007.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: Human; HeLa: Human; HT29: Human; A549: Human; COS7: monkey; Jurkat: human; MDCK: Canine; PC12: Rat; MCF7: human)](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Western-Blot-NBP2-45602-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CDKN2A.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0004.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0005.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0006.jpg)
![Western Blot: AKT2 Antibody (4H7) [NBP2-02231] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Western-Blot-NBP2-02231-img0003.jpg "Western Blot: AKT2 Antibody (4H7) [NBP2-02231] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.")
![Immunocytochemistry/Immunofluorescence: AKT2 Antibody (4H7) [NBP2-02231] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT2.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-02231-img0007.jpg)
![Immunohistochemistry-Paraffin: AKT2 Antibody (4H7) [NBP2-02231] - Staining of paraffin-embedded Human liver tissue using anti-AKT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunohistochemistry-Paraffin-NBP2-02231-img0006.jpg)
![Flow Cytometry: AKT2 Antibody (4H7) [NBP2-02231] - Analysis of Jurkat cells, using anti-AKT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Flow-Cytometry-NBP2-02231-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AKT2 Antibody (4H7) [NBP2-02231] - Immunofluorescent staining of HepG2 cells using anti-AKT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-02231-img0004.jpg)
![Immunohistochemistry-Paraffin: AKT2 Antibody (4H7) [NBP2-02231] - Staining of paraffin-embedded Human Kidney tissue using anti-AKT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunohistochemistry-Paraffin-NBP2-02231-img0005.jpg)
![Flow Cytometry: AKT2 Antibody (4H7) [NBP2-02231] - Analysis of Hela cells, using anti-AKT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Flow-Cytometry-NBP2-02231-img0001.jpg)
![Western Blot: CREB Antibody [NBP2-22516] - Analysis of GH4 cell extract.](http://images.novusbio.com/fullsize/CREB-Antibody-Western-Blot-NBP2-22516-img0001.jpg "Western Blot: CREB Antibody [NBP2-22516] - Analysis of GH4 cell extract.")
![Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP2-22516] - Analysis of CREB (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22516-img0004.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of human glioma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP2-22516-img0007.jpg)
![Chromatin Immunoprecipitation: CREB Antibody [NBP2-22516] - Analysis performed using cross-linked chromatin from colon carcinoma cells treated with serum. IP was performed using a multiplex microplate Matrix ChIP assay of a CREB polyclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data one in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of Egr1 or exon-1 or exon-2 of Egr1.Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM. A schematic representation of the Egr-1 locus is shown; xes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/CREB-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-22516-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP2-22516] - Analysis of CREB (green) showing staining in the nucleus of SK-N-MC cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22516-img0003.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of mouse brain tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP2-22516-img0005.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of human lung adenocarcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP2-22516-img0006.jpg)
![Western Blot: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK2.](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Western-Blot-NBP2-71259-img0002.jpg "Western Blot: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK2.")
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY JNK2.](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71259-img0007.jpg)
![Immunohistochemistry: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71259-img0006.jpg)
![Western Blot: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Western-Blot-NBP2-71259-img0001.jpg)
![Immunohistochemistry: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71259-img0003.jpg)
![Immunohistochemistry: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71259-img0004.jpg)
![Immunohistochemistry: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/JNK2-Antibody-OTI1A1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71259-img0005.jpg)
![Western Blot: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis using HSP90AB1 mouse mAb against Jurkat (1), A431 (2), Hela (3), A549 (4), HEK293 (5), K562 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-Western-Blot-NBP2-37590-img0002.jpg "Western Blot: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis using HSP90AB1 mouse mAb against Jurkat (1), A431 (2), Hela (3), A549 (4), HEK293 (5), K562 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of Hela cells using HSP90AB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-Immunocytochemistry-Immunofluorescence-NBP2-37590-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of paraffin-embedded kidney cancer tissues (left) and brain tissues (right) using HSP90AB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-Immunohistochemistry-NBP2-37590-img0005.jpg)
![Flow Cytometry: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of Hela cells using HSP90AB1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-Flow-Cytometry-NBP2-37590-img0004.jpg)
![ELISA: HSP90 beta Antibody (5G4) [NBP2-37590] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-ELISA-NBP2-37590-img0001.jpg)
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-449).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0003.jpg "Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-449).")
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were left untreated (C) or were treated with Cobalt Chloride / CoCl2 (H) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (clone H1alpha67; catalog# NB100-105).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0001.jpg)
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-479).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0002.jpg)
![Western Blot: Myosin heavy chain 11 Antibody (8A8J4) [NBP3-16323] - Western blot analysis of extracts of Mouse lung, using SMMHC/Myosin heavy chain 11 Rabbit mAb (NBP3-16323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-8A8J4-Western-Blot-NBP3-16323-img0004.jpg "Western Blot: Myosin heavy chain 11 Antibody (8A8J4) [NBP3-16323] - Western blot analysis of extracts of Mouse lung, using SMMHC/Myosin heavy chain 11 Rabbit mAb (NBP3-16323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Myosin heavy chain 11 Antibody (8A8J4) [NBP3-16323] - Western blot analysis of extracts of Rat lung, using SMMHC/Myosin heavy chain 11 Rabbit mAb (NBP3-16323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-8A8J4-Western-Blot-NBP3-16323-img0005.jpg)
![Immunohistochemistry: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Myosin heavy chain 11 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-5220251215495.jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Confocal imaging of paraffin-embedded Rat small intestine using Myosin heavy chain 11 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-52202511361230.jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Confocal imaging of paraffin-embedded Human colon using Myosin heavy chain 11 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-52202512541.jpg)
![Immunohistochemistry: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Myosin heavy chain 11 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-522025125499.jpg)
![Immunohistochemistry: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Immunohistochemistry analysis of paraffin-embedded Human lung tissue using Myosin heavy chain 11 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-5220251255579.jpg)
![Immunohistochemistry: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Myosin heavy chain 11 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-5220251242576.jpg)
![Immunohistochemistry: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Myosin heavy chain 11 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-52202511365199.jpg)
![Western Blot: ERK2 Antibody [NBP3-02974] - Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (NBP3-02974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ERK2-Antibody-Western-Blot-NBP3-02974-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ERK2 Antibody [NBP3-02974] - Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (NBP3-02974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ERK2 Antibody [NBP3-02974] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] ERK2 Rabbit pAb (NBP3-02974) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-02974-img0001.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody [NBP3-02974] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] ERK2 Rabbit pAb (NBP3-02974) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP3-02974-img0003.jpg)
![Western Blot: ERK2 Antibody [NBP3-02974] - Western blot analysis of extracts of various cell lines, using ERK2 antibody (NBP3-02974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ERK2 Antibody-Western Blot-NBP3-02974-img0005.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody [NBP3-02974] - Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] ERK2 Rabbit pAb (NBP3-02974) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP3-02974-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ERK2 Antibody - Azide and BSA Free [ERK2] - Western blot analysis of lysates from wild type (WT) and ERK2 knockout (KO) HeLa cells using [KO Validated] ERK2 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 90 s.](http://images.novusbio.com/fullsize/nbp3-02974_rabbit-polyclonal-erk2-antibody-5220251636384.jpg)
![Western Blot: ERK2 Antibody - Azide and BSA Free [ERK2] - Western blot analysis of various lysates using [KO Validated] ERK2 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 30 s.](http://images.novusbio.com/fullsize/nbp3-02974_rabbit-polyclonal-erk2-antibody-5220251632146.jpg)
![Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of various cell lines, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Western-Blot-NBP2-95252-img0008.jpg "Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of various cell lines, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunofluorescence analysis of U2OS cells using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-5220251639688.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Rat pancreas using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0007.jpg)
![Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of 293T cells, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/IRE1 alpha Antibody-Western Blot-NBP2-95252-img0009.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Human breast cancer using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0004.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Mouse kidney using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0005.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Rat kidney using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunofluorescence analysis of PC-12 cells using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-52202516334127.jpg)
![Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunofluorescence analysis of NIH/3T3 cells using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-5220251640267.jpg)
![Immunohistochemistry: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-5220251633413.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0012.jpg "Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase Antibody [NB600-861] - Analysis of HRP conjugate of NB600-861. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NB600-861-img0015.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0009.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0010.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4C. Secondary antibody: n/a. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0011.jpg)
