| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-454SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21679 | |||
| DyLight 488 | NBP1-77702 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1925SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54426-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11437 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB600-862-0.1mg | |||
| HRP | NB600-863-0.1mg | |||
![Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on mouse liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Western-Blot-NBP2-67572-img0007.jpg "Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on mouse liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of paraffin-embedded mouse brain tissue using anti-S100 beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunohistochemistry-Paraffin-NBP2-67572-img0003.jpg)
![Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Western-Blot-NBP2-67572-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0002.jpg)
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-449).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0003.jpg "Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-449).")
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were left untreated (C) or were treated with Cobalt Chloride / CoCl2 (H) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (clone H1alpha67; catalog# NB100-105).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0001.jpg)
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-479).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0002.jpg)
![Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD4 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PHD4-HIF-Prolyl-Hydroxylase-4-Antibody-Western-Blot-NB100-295-img0007.jpg "Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD4 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Analysis of human PHD4, using NB100-295. Samples: whole cell lysate from HeLa cells, normoxic and hypoxic, respectively.](http://images.novusbio.com/fullsize/PHD4-HIF-Prolyl-Hydroxylase-4-Antibody-Western-Blot-NB100-295-img0004.jpg)
![Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Whole cell lysate (25 mcg/lane) from human glioblastoma tumor cell lines or in vitro translated PHD4. Antibody: Affinity purified rabbit anti-PHD4 (Cat. No. NB100-295) used at 2 mcg/ml. Detection: Chemiluminescence](http://images.novusbio.com/fullsize/PHD4-HIF-Prolyl-Hydroxylase-4-Antibody-Western-Blot-NB100-295-img0006.jpg)
![Western Blot: VG5Q Antibody [NB100-454] - Human VG5Q detected in HT1080 non-transfected cell lysate (NTC) and HT1080 transfected cell lysate (TC) using NB 100-454.](http://images.novusbio.com/fullsize/VG5Q-Antibody-Western-Blot-NB100-454-img0003.jpg "Western Blot: VG5Q Antibody [NB100-454] - Human VG5Q detected in HT1080 non-transfected cell lysate (NTC) and HT1080 transfected cell lysate (TC) using NB 100-454.")
![Immunohistochemistry-Paraffin: VG5Q Antibody [NB100-454] - VG5Q was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human VG5Q polyclonal Antibody (Catalog # NB100-454) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in hepatocytes.](http://images.novusbio.com/fullsize/VG5Q-Antibody-Immunohistochemistry-Paraffin-NB100-454-img0004.jpg)
![Western Blot: TRIB3 Antibody [NB100-56398] - Analysis of TRIB3 in human liver lysate.](http://images.novusbio.com/fullsize/TRIB3-Antibody-Western-Blot-NB100-56398-img0002.jpg "Western Blot: TRIB3 Antibody [NB100-56398] - Analysis of TRIB3 in human liver lysate.")
![Western Blot: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates. Load: 15 ug per lane. Primary antibody: AKT phospho Thr308 antibody at a 1:4000 dilution in TBS with 3% BSA, for 3 h at 4 C. Secondary antibody: peroxidase conjugated Gt-a-Mouse IgG (Fc) at a 1:40,000 dilution for 1 h at 4 C. Block: 3% BSA in TBS for 30 min at RT. Predicted/Observed size: (indicated by arrowhead at 56 kDa). Other band(s): unspecific.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Western-Blot-NBP1-69924-img0011.jpg "Western Blot: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates. Load: 15 ug per lane. Primary antibody: AKT phospho Thr308 antibody at a 1:4000 dilution in TBS with 3% BSA, for 3 h at 4 C. Secondary antibody: peroxidase conjugated Gt-a-Mouse IgG (Fc) at a 1:40,000 dilution for 1 h at 4 C. Block: 3% BSA in TBS for 30 min at RT. Predicted/Observed size: (indicated by arrowhead at 56 kDa). Other band(s): unspecific.")
![Immunocytochemistry/Immunofluorescence: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - IHC-P analysis of FFPE human prostate tissue using AKT1 phospho Thr308 antibody at 20 ug/mL. Left panel: 20X magnification. Right panel: 40X magnification. Heat antigen retrieval in citrate buffer pH 6.2. Secondary antibody: streptavidin-HRP at 10 ug/mL. Antibody as precipitated red signal with hemotoxylin purple nuclear counterstain. Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Immunocytochemistry-Immunofluorescence-NBP1-69924-img0014.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - IHC analysis. Detection of phosphorylated AKT phospho Thr308 in human FFPE brain cerebellum tissue (40X). AKT1 phospho Thr308 antibody was used at 20 ug/mL. The image shows strong staining of Purkinje neurons. Red: AKT1 pT308. Purple: Hematoxylin nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Immunohistochemistry-Paraffin-NBP1-69924-img0003.jpg)
![Western Blot: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - WB analysis using the Biotin conjugate of AKT1 pT308 antibody. Detection of Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: GST tagged AKT1 inactive recombinant protein. Load: 25 ng per lane. AKT pT308 Biotin conjugated antibody at 1:1000 overnight at 4C. Secondary antibody: HRP Streptavidin.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Western-Blot-NBP1-69924-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - ICC/IF analysis using the DyLight 488 conjugate of AKT1 pT308, NBP1-69924. Histone detection using DyLight 488 conjugate (green). Anti-Tubulin was detected using DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Immunocytochemistry-Immunofluorescence-NBP1-69924-img0006.jpg)
![ELISA: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - ELISA of AKT1 phospho Thr308 Biotin conjugated antibody. Antigen: Unconjugated AKT1 phospho Thr308 and AKT1 non-phospho Thr308. Coating amount: 0.1 ug per well. Primary antibody: AKT1 phospho Thr308 Biotin conjugated antibody at 5 ug/mL. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL AKT1 phospho Thr308 Biotin conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10,000. Substrate: TMB](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-ELISA-NBP1-69924-img0013.jpg)
![Western Blot: JNK1 Antibody [NB100-1925] - Detection of JNK-1 using NB100-1925.](http://images.novusbio.com/fullsize/JNK1-Antibody-Western-Blot-NB100-1925-img0004.jpg "Western Blot: JNK1 Antibody [NB100-1925] - Detection of JNK-1 using NB100-1925.")
![Western Blot: S100B Antibody (S100B/1706R) [NBP2-53188] - Western blot analysis of human brain tissue lysate using S100B antibody (S100B/1706R).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Western-Blot-NBP2-53188-img0003.jpg "Western Blot: S100B Antibody (S100B/1706R) [NBP2-53188] - Western blot analysis of human brain tissue lysate using S100B antibody (S100B/1706R).")
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1706R) [NBP2-53188] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Rabbit Monoclonal Antibody (S100B/1706R).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Immunohistochemistry-Paraffin-NBP2-53188-img0001.jpg)
![Protein Array: S100B Antibody (S100B/1706R) [NBP2-53188] - Analysis of Protein Array containing more than 19,000 full-length human proteins using S100B Antibody (S100B/1706R) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Protein-Array-NBP2-53188-img0002.jpg)
![Immunohistochemistry-Paraffin: VEGF Antibody (VEGF/1063) [NBP2-45235] - Human Tonsil stained with VEGF Monoclonal Antibody (VEGF/1063).](http://images.novusbio.com/fullsize/VEGF-Antibody-VEGF-1063-Immunohistochemistry-Paraffin-NBP2-45235-img0003.jpg "Immunohistochemistry-Paraffin: VEGF Antibody (VEGF/1063) [NBP2-45235] - Human Tonsil stained with VEGF Monoclonal Antibody (VEGF/1063).")
![Immunohistochemistry-Paraffin: VEGF Antibody (VEGF/1063) [NBP2-45235] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with VEGF Antibody (VEGF/1063).](http://images.novusbio.com/fullsize/VEGF-Antibody-VEGF-1063-Immunohistochemistry-Paraffin-NBP2-45235-img0006.jpg)
![Immunohistochemistry-Paraffin: VEGF Antibody (VEGF/1063) [NBP2-45235] - Formalin-fixed, paraffin-embedded human Tonsil stained with VEGF Antibody (VEGF/1063).](http://images.novusbio.com/fullsize/VEGF-Antibody-VEGF-1063-Immunohistochemistry-Paraffin-NBP2-45235-img0005.jpg)
![Immunohistochemistry-Paraffin: VEGF-C Antibody [NBP2-20853] - Human hepatoma, using VEGFC antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/VEGFC-Antibody-Immunohistochemistry-Paraffin-NBP2-20853-img0003.jpg "Immunohistochemistry-Paraffin: VEGF-C Antibody [NBP2-20853] - Human hepatoma, using VEGFC antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![<b>Genetic Strategies Validation. </b>Western Blot: VEGF-C Antibody [NBP2-20853] - Sample (20 ug of whole cell lysate) A: TL1 whole cell lysate B: TL1 knock down VEGF-C whole cell lysate 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/VEGFC-Antibody-Western-Blot-NBP2-20853-img0001.jpg)
![Western Blot: VEGF-C Antibody [NBP2-20853] - 50 ug mouse lung lysate/extract 12% SDS-PAGE VEGFC antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/VEGFC-Antibody-Western-Blot-NBP2-20853-img0002.jpg)
![Western Blot: VEGF-C Antibody [NBP2-20853] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VEGF-C antibody (NBP2-20853) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20853_rabbit-polyclonal-vegf-c-antibody-910202420104436.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0012.jpg "Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase Antibody [NB600-861] - Analysis of HRP conjugate of NB600-861. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NB600-861-img0015.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0009.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0010.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4C. Secondary antibody: n/a. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0011.jpg)
![SDS-Page: CREB Recombinant Protein [H00001385-P01]](http://images.novusbio.com/fullsize/CREB-Recombinant-Protein-SDS-Page-H00001385-P01-img0001.jpg "SDS-Page: CREB Recombinant Protein [H00001385-P01]")