| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Unpurified | NB100-185SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89443-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52671-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89065-25ul | |||
![Western Blot: Myosin heavy chain 11 Antibody (8A8J4) [NBP3-16323] - Western blot analysis of extracts of Mouse lung, using SMMHC/Myosin heavy chain 11 Rabbit mAb (NBP3-16323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-8A8J4-Western-Blot-NBP3-16323-img0004.jpg "Western Blot: Myosin heavy chain 11 Antibody (8A8J4) [NBP3-16323] - Western blot analysis of extracts of Mouse lung, using SMMHC/Myosin heavy chain 11 Rabbit mAb (NBP3-16323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Myosin heavy chain 11 Antibody (8A8J4) [NBP3-16323] - Western blot analysis of extracts of Rat lung, using SMMHC/Myosin heavy chain 11 Rabbit mAb (NBP3-16323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-8A8J4-Western-Blot-NBP3-16323-img0005.jpg)
![Immunohistochemistry: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Myosin heavy chain 11 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-5220251215495.jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Confocal imaging of paraffin-embedded Rat small intestine using Myosin heavy chain 11 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-52202511361230.jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Confocal imaging of paraffin-embedded Human colon using Myosin heavy chain 11 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-52202512541.jpg)
![Immunohistochemistry: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Myosin heavy chain 11 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-522025125499.jpg)
![Immunohistochemistry: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Immunohistochemistry analysis of paraffin-embedded Human lung tissue using Myosin heavy chain 11 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-5220251255579.jpg)
![Immunohistochemistry: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Myosin heavy chain 11 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-5220251242576.jpg)
![Immunohistochemistry: Myosin heavy chain 11 Antibody (0O4O4) [NBP3-16323] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Myosin heavy chain 11 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16323_rabbit-myosin-heavy-chain-11-mab-0o4o4-52202511365199.jpg)
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Western-Blot-NBP2-22171-img0004.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Immunocytochemistry-Immunofluorescence-NBP2-22171-img0002.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Immunohistochemistry-Paraffin-NBP2-22171-img0003.jpg)
![Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Flow-Cytometry-NBP2-22171-img0001.jpg)
![Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250](http://images.novusbio.com/fullsize/PARP2-Antibody-Western-Blot-NB100-185-img0001.jpg "Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250")
![Western Blot: PARP2 Antibody [NB100-185] - Western blot of analysis of PARP2 in 3T3 cell lysate using NB100-185 at 1:250 dilution.](http://images.novusbio.com/fullsize/PARP2-Antibody-Western-Blot-NB100-185-img0002.jpg)
![Western Blot: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of COX-1 on different lysates with Rabbit anti-COX-1 antibody at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 69 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Western-Blot-NBP2-67643-img0011.jpg "Western Blot: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of COX-1 on different lysates with Rabbit anti-COX-1 antibody at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 69 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunocytochemistry-Immunofluorescence-NBP2-67643-img0010.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-COX-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0012.jpg)
![Flow Cytometry: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of Hela cells with COX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Flow-Cytometry-NBP2-67643-img0001.jpg)
![Western Blot: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of COX1 on different lysates using anti-COX1 antibody at 1/1,000 dilution. Positive control: Lane 1: C2C12 Lane 2: A431](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Western-Blot-NBP2-67643-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunocytochemistry-Immunofluorescence-NBP2-67643-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunocytochemistry-Immunofluorescence-NBP2-67643-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunocytochemistry-Immunofluorescence-NBP2-67643-img0009.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded human breast cancer tissue using anti-COX1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0004.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded mouse brain tissue using anti-COX1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0005.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded mouse skin tissue using anti-COX1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0006.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded mouse stomach tissue using anti-COX1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0007.jpg)
![Western Blot: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of BNIP3 on mouse kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Western-Blot-NBP2-67192-img0009.jpg "Western Blot: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of BNIP3 on mouse kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (JA71-10) [NBP2-67192] - Staining BNIP3 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunocytochemistry-Immunofluorescence-NBP2-67192-img0008.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded mouse brain tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (JA71-10) [NBP2-67192] - Staining BNIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunocytochemistry-Immunofluorescence-NBP2-67192-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (JA71-10) [NBP2-67192] - Staining BNIP3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunocytochemistry-Immunofluorescence-NBP2-67192-img0007.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0002.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded human colon cancer tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0003.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded human kidney tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0004.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of GSK-3 alpha/beta [p Tyr216, p Tyr279] on mouse brain tissue lysates with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279] antibody at 1:500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 46/51 kDa Observed band size: 46/51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0013.jpg "Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of GSK-3 alpha/beta [p Tyr216, p Tyr279] on mouse brain tissue lysates with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279] antibody at 1:500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 46/51 kDa Observed band size: 46/51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0003.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0017.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on 293T cell lysates.Lane 1: 293T cells, whole cell lysate, 10ug/lane Lane 2: 293T cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes :Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0011.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: A431 cell lysate Lane 2: Hela cell lysate](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0002.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0004.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0005.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-GSK-3 alpha/beta (p Tyr216+Y279) antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0014.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0015.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279] antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0016.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human hepatoma with rabbit monoclonal antibody (1:2000)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89443-img0003.jpg "Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human hepatoma with rabbit monoclonal antibody (1:2000)")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Osteopontin/OPN Antibody (001) [NBP2-89443] - Analysis of purified anti-human Spp1 on U937 cells. U937 cells were treated and stained with Purified Rabbit anti-Spp1 (Bold line hisgram), To demonstrate specificity of staining the binding of anti-human Spp1 was blocked by the preincubation of the purified antibody with molar excess of recombinant human Spp1 (5 ug,) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-001-Flow-Cytometry-NBP2-89443-img0001.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human kidney with rabbit monoclonal antibody (1:2000)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89443-img0002.jpg)
![Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibodydiluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Western-Blot-NBP1-32140-img0011.jpg "Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibodydiluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32140-img0015.jpg)
![Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Western-Blot-NBP1-32140-img0008.jpg)
![Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Rat tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Western-Blot-NBP1-32140-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32140-img0005.jpg)
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Paraffin-embedded CL1-0 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP1-32140-img0006.jpg)
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Paraffin-embedded mouse fore brain. ORP150 antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP1-32140-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - Meta-analysis of significant metastatic upregulated proteins in acid exosomes through Prognoscan. a Kaplan-Meyer curves depicting the Overall Survival of metastatic melanoma patients with high (red lines) or low (green lines) expression of the genes listed in Table 1 that resulted statistically significant. Mentel-Cox p-values for each plot are showed. b Western blotting analysis of ctr & pH 6.0 exosomes (20 μg). Cell lysate was used as positive control. Expression of CFL, GSN & HSP90 alpha beta is shown. Ponceau red is shown for equal exosome protein amount. c confocal immunofluorescence analysis with the indicated antibodies on primary (apical & mid region), & metastatic melanoma biopsies from the same patient. Ki67 labeling was used as internal control. Red, CFL, GSN, HYOU1; blue, DAPI; green, Ki67. Bar 50 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30290833), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32140_rabbit-polyclonal-orp150-hsp12a-antibody-31020241615469.jpg)
![ORP150/HSP12A antibody [C2C3], C-term detects ORP150/HSP12A protein at endoplasmic reticulum by immunofluorescent analysis.<br>Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: ORP150/HSP12A protein stained by ORP150/HSP12A antibody [C2C3], C-term (NBP1-32140) diluted at 1:200.<br>Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.<br>Blue: Hoechst 33342 staining.<br>](http://images.novusbio.com/fullsize/nbp1-32140_rabbit-polyclonal-orp150-hsp12a-antibody-immunocytochemistry-immunofluorescence-141120251841517.jpg)
![ORP150/HSP12A antibody [C2C3], C-term detects ORP150/HSP12A protein at cytoplasm by immunofluorescent analysis.<br>Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: ORP150/HSP12A protein stained by ORP150/HSP12A antibody [C2C3], C-term (NBP1-32140) diluted at 1:1000.<br>Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.<br>Blue: Hoechst 33342 staining.<br>](http://images.novusbio.com/fullsize/nbp1-32140_rabbit-polyclonal-orp150-hsp12a-antibody-immunocytochemistry-immunofluorescence-141120251839103.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP1-89065] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89065-img0011.jpg "Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP1-89065] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human stomach shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NBP1-89065-img0019.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NBP1-89065-img0016.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NBP1-89065-img0017.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human pancreas shows very weak and few nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NBP1-89065-img0018.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M10), clone 1F1. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-1F1-Western-Blot-H00000759-M10-img0005.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M10), clone 1F1. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in human lung cancer.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-1F1-Western-Blot-H00000759-M10-img0004.jpg)
![Sandwich ELISA: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Detection limit for recombinant GST tagged CA1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-1F1-Sandwich-ELISA-H00000759-M10-img0003.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (7G12) [H00000759-M07] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M07), clone 7G12. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-7G12-Western-Blot-H00000759-M07-img0004.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody (7G12) [H00000759-M07] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M07), clone 7G12. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (7G12) [H00000759-M07] - Analysis of CA1 expression in human pancreas.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-7G12-Western-Blot-H00000759-M07-img0003.jpg)
![ELISA: Carbonic Anhydrase I/CA1 Antibody (7G12) [H00000759-M07] - Detection limit for recombinant GST tagged CA1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-7G12-ELISA-H00000759-M07-img0002.jpg)