| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92688SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33842-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89159-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90208-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89702-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86833-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21678-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88103-25ul | |||
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 4. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-811202420592116.jpg "Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 4. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.")
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 3. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-811202420594212.jpg)
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 2. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-81120242059212.jpg)
![Western Blot: MyoD Antibody (5.2F) [NBP3-30815] - Left: Immunohistochemical staining using MyoD antibody on formalin fixed, paraffin embedded human rhabdomyosarcoma. Right: Western blot using 60 ng of purified MyoD protein with MyoD antibody at 2 ug/ml and detected using goat anti-mouse HRP and visualized with Pierce West-Femto substrate.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-811202420592184.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Knockout-Validated-NBP1-92688-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2A52) [NBP1-92688] - Immunocytochemistry/Immunofluorescence: [NBP1-92688] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Immunocytochemistry-Immunofluorescence-NBP1-92688-img0005.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Analysis of cell and whole brain tissue lysates using mouse mAb to vimentin, NBP1-92688, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] C6, and [7] rat brain. The band at about 50kDa mark corresponds to vimentin protein. The antibody does not recognize mouse vimentin (not shown).](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Western-Blot-NBP1-92688-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Frizzled-2 Antibody [NBP2-33842] - Immunofluorescent staining of human cell line CACO-2 shows localization to nuclear bodies & cell junctions.](http://images.novusbio.com/fullsize/Frizzled-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33842-img0005.jpg "Immunocytochemistry/Immunofluorescence: Frizzled-2 Antibody [NBP2-33842] - Immunofluorescent staining of human cell line CACO-2 shows localization to nuclear bodies & cell junctions.")
![Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP2-33842] - Staining of human testis shows weak to moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Frizzled-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33842-img0008.jpg)
![Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP2-33842] - Staining of human cerebral cortex shows no positivity in neurons.](http://images.novusbio.com/fullsize/Frizzled-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33842-img0006.jpg)
![Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP2-33842] - Staining of human heart muscle shows moderate to strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Frizzled-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33842-img0007.jpg)
![Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP2-33842] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Frizzled-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33842-img0009.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody [NBP1-89159] - IHC analysis of human seminal vesicle and skeletal muscle tissues using anti-AKT1 antibody. Corresponding AKT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AKT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89159-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AKT1 Antibody [NBP1-89159] - IHC analysis of human seminal vesicle and skeletal muscle tissues using anti-AKT1 antibody. Corresponding AKT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AKT1 Antibody [NBP1-89159] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & microtubules.](http://images.novusbio.com/fullsize/AKT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89159-img0007.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody [NBP1-89159] - IHC staining of human seminal vesicle shows high expression.](http://images.novusbio.com/fullsize/AKT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89159-img0008.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody [NBP1-89159] - IHC staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/AKT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89159-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Jagged 1 Antibody [NBP1-90208] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90208-img0012.jpg)
![Immunohistochemistry-Paraffin: Jagged 1 Antibody [NBP1-90208] - Staining of human lymph node shows no cytoplasmic positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90208-img0011.jpg)
![Immunohistochemistry-Paraffin: Jagged 1 Antibody [NBP1-90208] - Staining of human duodenum shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90208-img0010.jpg)
![Immunohistochemistry-Paraffin: Jagged 1 Antibody [NBP1-90208] - Staining of human skin shows weak cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90208-img0013.jpg)
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NBP1-89702] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NBP1-89702-img0010.jpg "Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NBP1-89702] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NBP1-89702] - Staining of human placenta shows moderate cytoplasmic and membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunohistochemistry-Paraffin-NBP1-89702-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: ZIC1 Antibody [NBP1-86833] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86833-img0011.jpg "Immunocytochemistry/Immunofluorescence: ZIC1 Antibody [NBP1-86833] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Immunohistochemistry-Paraffin-NBP1-86833-img0015.jpg)
![Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in molecular and granular layer.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Immunohistochemistry-Paraffin-NBP1-86833-img0012.jpg)
![Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Immunohistochemistry-Paraffin-NBP1-86833-img0013.jpg)
![Immunohistochemistry-Paraffin: ZIC1 Antibody [NBP1-86833] - Staining of human lymph node shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Immunohistochemistry-Paraffin-NBP1-86833-img0014.jpg)
![Immunohistochemistry: SPRY2 Antibody [NBP2-13158] - Tissue: liver. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-sprouty 2 at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/SPRY2-Antibody-Immunohistochemistry-NBP2-13158-img0003.jpg "Immunohistochemistry: SPRY2 Antibody [NBP2-13158] - Tissue: liver. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-sprouty 2 at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.")
![Immunohistochemistry-Paraffin: SPRY2 Antibody [NBP2-13158] - Immunohistochemistry-Paraffin: Sprouty 2 - Tissue: human kidney clear cell tumor. Fixation: formalin fixed, paraffin embedded. Antigen retrieval: yes. Primary antibody: Sprouty2 antibody at 1:250. Secondary antibody: alexafluor 488 secondary at 1:500 overnight at 4C. Localization: nucleus and cytoplasm. Staining: green = sprouty2, Blue = dapi, red= vhl.](http://images.novusbio.com/fullsize/SPRY2-Antibody-Immunohistochemistry-Paraffin-NBP2-13158-img0001.jpg)
![Immunohistochemistry: SPRY2 Antibody [NBP2-13158] - Tissue: kidney. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-sprouty 2 at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/SPRY2-Antibody-Immunohistochemistry-NBP2-13158-img0002.jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-29820241420955.jpg "Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33143) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-298202413585572.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunocytochemistry analysis of HeLa cells labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 Alpha 1 antibody (NBP3-33143) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-29202410234287.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Western blot analysis of Sodium Potassium ATPase Alpha 1 on different lysates with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/100,000 dilution. Lane 1: HeLa cell lysate (no heat) (20 µg/Lane) Lane 2: HT-29 cell lysate (no heat) (20 µg/Lane) Lane 3: HepG2 cell lysate (no heat) (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (no heat) (20 µg/Lane) Lane 5: L-929 cell lysate (no heat) (20 µg/Lane) Lane 6: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 7: Rat brain tissue lysate (no heat) (20 µg/Lane) Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-392024848504.jpg)
![Immunohistochemistry: EGFR Antibody (PD00-75) [NBP3-32255] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-EGFR antibody (NBP3-32255) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32255) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-29820241420932.jpg "Immunohistochemistry: EGFR Antibody (PD00-75) [NBP3-32255] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-EGFR antibody (NBP3-32255) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32255) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: EGFR Antibody (PD00-75) [NBP3-32255] - Immunocytochemistry analysis of A431 cells labeling EGFR with Rabbit anti-EGFR antibody (NBP3-32255) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-2920241192377.jpg)
![Flow Cytometry: EGFR Antibody (PD00-75) [NBP3-32255] - Flow cytometric analysis of A431 cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32255, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-29202416372141.jpg)
![Western Blot: EGFR Antibody (PD00-75) [NBP3-32255] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32255) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: MDA-MB-468 cell lysate Lane 4: MCF7 cell lysate (low expression) Lysates/proteins at 15 µg/Lane. Predicted band size: 134 kDa Observed band size: 150 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-3920248485012.jpg)
![Immunohistochemistry: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21678.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-5720231526123.jpg "Immunohistochemistry: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21678.")
![Immunocytochemistry/Immunofluorescence: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunofluorescent analysis of MCF-7 cells, using NBP3-21678.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-57202317161873.jpg)
![Western Blot: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Western blot analysis using NBP3-21678 in (1) A549 cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate; (4) Rat heart lysate; (5) Rat kidney lysate.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-5720231558486.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody [NBP1-88103] - Staining in human tonsil and pancreas tissues using CD45 antibody [NBP1-88103]. Corresponding CD45 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-Paraffin-NBP1-88103-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD45 Antibody [NBP1-88103] - Staining in human tonsil and pancreas tissues using CD45 antibody [NBP1-88103]. Corresponding CD45 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD45 Antibody [NBP1-88103] - Staining of human pancreas with CD45 antibody [NBP1-88103] shows low expression as expected.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-Paraffin-NBP1-88103-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody [NBP1-88103] - Staining of human tonsil with CD45 antibody [NBP1-88103] shows high expression.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-Paraffin-NBP1-88103-img0006.jpg)