| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47949-0.1mg | |||
| IHC-Prediluted | NBP2-48190 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52710-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21678 | |||
| DyLight 488 | NBP1-77701 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80786 | |||
| Azide and BSA Free | NBP2-80786SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47740-0.1mg | |||
| IHC-Prediluted | NBP2-48202 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26856-25ul | |||
| Azide and BSA Free | NBP3-44310 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43911 | |||
| BSA Free | NBP2-52952-25ul | |||
![Immunohistochemistry-Paraffin: Vimentin Antibody (LN-6) [NBP2-44832] - Human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).](http://images.novusbio.com/fullsize/Vimentin-Antibody-LN-6-Immunohistochemistry-Paraffin-NBP2-44832-img0002.jpg "Immunohistochemistry-Paraffin: Vimentin Antibody (LN-6) [NBP2-44832] - Human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).")
![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NBP2-16025] - Human gastric cancer, using CTGF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-16025-img0007.jpg "Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NBP2-16025] - Human gastric cancer, using CTGF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: CTGF/CCN2 Antibody [NBP2-16025] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Western-Blot-NBP2-16025-img0011.jpg)
![Immunohistochemistry-Frozen: Substance P Antibody (NC1/34) [NB100-65219] - Opioid R/OPRM1 (green) and Substance P (red) were detected in frozen mouse spinal cord and DRG tissue by fluorescent multiplex IHC. Mu Opioid R/OPRM1 Antibody (NB100-1620), used at 1:100, and Substance P Antibody (NB100-65219), used at 1:50, were applied to the tissue and simultaneously incubated at 4C overnight. Anti-Rabbit Alexa Fluor(R) 488-conjugated secondary antibody and anti-Rat NL557-conjugated secondary antibody (NL013) were applied to the tissue, and simultaneously incubated at room temperature, protected from light for 1 hour. Coverslips were applied to the tissue slide along with mounting media containing DAPI (blue) and imaged using a 4X objective lens.](http://images.novusbio.com/fullsize/Substance-P-Antibody-NC1-34-Immunohistochemistry-Frozen-NB100-65219-img0001.jpg "Immunohistochemistry-Frozen: Substance P Antibody (NC1/34) [NB100-65219] - Opioid R/OPRM1 (green) and Substance P (red) were detected in frozen mouse spinal cord and DRG tissue by fluorescent multiplex IHC. Mu Opioid R/OPRM1 Antibody (NB100-1620), used at 1:100, and Substance P Antibody (NB100-65219), used at 1:50, were applied to the tissue and simultaneously incubated at 4C overnight. Anti-Rabbit Alexa Fluor(R) 488-conjugated secondary antibody and anti-Rat NL557-conjugated secondary antibody (NL013) were applied to the tissue, and simultaneously incubated at room temperature, protected from light for 1 hour. Coverslips were applied to the tissue slide along with mounting media containing DAPI (blue) and imaged using a 4X objective lens.")
![Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis using AKT1 antibody against NIH/3T3 (1), Hela (2),COS7 (3) and Jurkat (4) cell lysate.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Western-Blot-NBP1-51602-img0006.jpg "Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis using AKT1 antibody against NIH/3T3 (1), Hela (2),COS7 (3) and Jurkat (4) cell lysate.")
![Immunohistochemistry-Paraffin: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of human breast carcinoma using AKT1 antibody at 1:50. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Immunohistochemistry-Paraffin-NBP1-51602-img0010.jpg)
![Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of extracts from COS cells using AKT1 antibody at 1:1000. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Western-Blot-NBP1-51602-img0008.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of human endometrial cancer using AKT1 antibody at 1:25. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Immunohistochemistry-Paraffin-NBP1-51602-img0009.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0009.jpg "Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0007.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-NCAM-1/CD56 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0011.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of SH-SY-5Y cells with NCAM antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Flow-Cytometry-NBP2-66968-img0001.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM on different lysates using anti-NCAM antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Human brain](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0006.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on SH-SY5Y cell lysates with Rabbit anti-NCAM-1/CD56 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 120/140 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0003.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human tonsil tissue using anti-NCAM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0004.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded zebrafish kidney tissue using anti-NCAM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0005.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human tonsil tissue using anti-NCAM-1/CD56 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0010.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2/CBFA1 on different lysates with Rabbit anti-RUNX2/CBFA1 antibody at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Western-Blot-NBP2-67777-img0005.jpg "Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2/CBFA1 on different lysates with Rabbit anti-RUNX2/CBFA1 antibody at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Staining RUNX2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunocytochemistry-Immunofluorescence-NBP2-67777-img0002.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded mouse bone tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0009.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse spleen tissue lysateLane 2: U937 cell lysate Lane 3: HL-60 cell lysate](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Western-Blot-NBP2-67777-img0003.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human tonsil tissue using anti-RUNX2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX2/CBFA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human tonsil tissue using anti-RUNX2/CBFA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0006.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0011.jpg "Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.")
![Flow Cytometry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the DyLight 488 conjugate of AKT1 phospho Ser473 antibody. Image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Flow-Cytometry-NBP1-69923-img0021.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). A: Lane 1) PDGF stimulated NIH 3T3 cells [10ul]. Lane 2) NIH 3T3 cells [10ul]. Lane 3) Hela whole cell lysate [10ul] (weak signal). B: Lane 4) GST negative control protein [100ng]. Lane 5) GST negative control protein [25ng]. Lane 6) AKT 1 recombinant protein [100ng]. Lane 7) AKT 1 recombinant protein [25ng].Block: 5% BSA overnight at 4C.Primary antibody: Monoclonal anti-AKT antibody used at 1:1000 for overnight at 4C.Secondary antibody: HRP Conjugated goat anti-mouse 1:25K for 45 min at RT.Detection: TMB for 20 minutes, rinsed with deionized water, dried and scanned on conventional flatbed scanner.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0045.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - High resolution STED immunofluorescence nanoscopy of AKT11 [p Ser473] antibody (17F6.B11). Tissue: A431 cells. The merge images (A) and at high magnification (B) show phosphorylated AKT1 colocalized with the distal microtubules. Fixation: 4% paraformaldehyde for 5 min and after washes blocked with 10% NGS/0.2% Triton X-100 for 30 min.Antigen retrieval: serum deprivation for 12 h.Primary antibody: AKT1 pS473 antibody at 10 ug/mL and alpha-tubulin (cyan) at 1.4 ug/mL for 1 h at RT.Secondary antibody: Atto 647N anti-Mouse IgG (ATTO TEC GmbH), and DyLight(TM)488 anti-Rabbit IgG were used at 1.0 ug/mL for 1h at RT for indirect detection.Localization: AKT1 pS473 is in the cytoplasm and also organized at the periphery of the cell.Staining: AKT1 pS473 as red signal with bis-benzimide (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0040.jpg)
![Immunohistochemistry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunohistochemistry of AKT11 [p Ser473] antibody (17F6.B11). Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: AKT1 pS473 antibody at 20 ug/mL for 1 h at RT.Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.Localization: AKT1 pS473 is nuclear and occasionally cytoplasmic.Staining: AKT1 pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-NBP1-69923-img0046.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: untreated NIH/3T3 cell lysates. Lane 2: detects phosphorylated AKT1 (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 ug per lane.Primary antibody: AKT1 pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4 C.Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4 C with FemtoMax(TM) enhanced chemiluminescent reagent.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0042.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane.Primary antibody: AKT1pS473 antibody at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 pS473 Monoclonal Antibody at 1:10,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C.Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0043.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated AKT11, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated AKT11 (red band). Load: 10 ug per lane.Primary antibody: rabbit anti-AKT1 (pan) and mouse anti-AKT1 pS473 specific antibodies at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM) 549 conjugated anti-rabbit IgG (green) and DyLight(TM) 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0044.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of DyLight 488 conjugate of NBP1-69923. This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0035.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923]](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunofluorescence Microscopy of AKT11 [p Ser473] antibody (17F6.B11) using STED nanoscopy to evaluate AKT1 activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins.A massive increase in AKT1-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin.Staining: Panel A shows STED data (AKT1-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT1 is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT1pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0039.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of Biotin conjugate of NBP1-69923. 20 ug/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear count](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0034.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of FFPE prostate tissue using AKT1 phospho Ser473 antibody (left panel). Negative control (right panel). Antigen retrieval with heat and pressure in citrate buffer pH 6.2. AKT1 phospho Ser473 antibody at 20 ug/mL. Secondary antibody Streptavidin-HRP at 10 ug/mL. Hematoxylin nuclear counterstain (purple). Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0036.jpg)
![Western Blot of Mouse Anti-AKTpS473 antibody. Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane. Primary antibody: AKTpS473 antibody at 1:400 for overnight at 4C. Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 [p Ser473] Antibody (17F6.B11) at 1:10,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C. Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/nbp1-69923_mouse-monoclonal-akt1-p-ser473-antibody-17f6-b11-255202313136.jpg)
![Western Blot: SynCAM/CADM1 Antibody (41N3D3) [NBP2-14841] - Analysis of CADM1 recombinant protein using CADM1 antibody at 1 ug/ml. Due to glycosylation, CADM1 can be visualized between 40-80 kD in western blot.](http://images.novusbio.com/fullsize/SynCAM-CADM1-Antibody-41N3D3-Western-Blot-NBP2-14841-img0003.jpg "Western Blot: SynCAM/CADM1 Antibody (41N3D3) [NBP2-14841] - Analysis of CADM1 recombinant protein using CADM1 antibody at 1 ug/ml. Due to glycosylation, CADM1 can be visualized between 40-80 kD in western blot.")
![Immunohistochemistry-Paraffin: EGFR Antibody (31G7) [NBP2-45148] - Human Placenta stained with EGFR Monoclonal Antibody (31G7).](http://images.novusbio.com/fullsize/EGFR-Antibody-31G7-Immunohistochemistry-Paraffin-NBP2-45148-img0001.jpg "Immunohistochemistry-Paraffin: EGFR Antibody (31G7) [NBP2-45148] - Human Placenta stained with EGFR Monoclonal Antibody (31G7).")
![Protein Array: EGFR Antibody (31G7) [NBP2-45148] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (31G7) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EGFR-Antibody-31G7-Protein-Array-NBP2-45148-img0003.jpg)
![Western Blot: EGFR Antibody (CL0815) [NBP2-52952] - Lane 1: Marker [kDa], Lane 2: Human cell line A-431](http://images.novusbio.com/fullsize/EGFR-Antibody-CL0815-Western-Blot-NBP2-52952-img0004.jpg "Western Blot: EGFR Antibody (CL0815) [NBP2-52952] - Lane 1: Marker [kDa], Lane 2: Human cell line A-431")
![Immunohistochemistry-Paraffin: EGFR Antibody (CL0815) [NBP2-52952] - Staining of human ventricular cancer shows moderate membranous immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-CL0815-Immunohistochemistry-Paraffin-NBP2-52952-img0003.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody (CL0815) [NBP2-52952] - Staining of human placenta shows strong membranous positivity in trophoblast.](http://images.novusbio.com/fullsize/EGFR-Antibody-CL0815-Immunohistochemistry-Paraffin-NBP2-52952-img0001.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody (CL0815) [NBP2-52952] - Staining of human tonsil shows moderate membranous immunoreactivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-CL0815-Immunohistochemistry-Paraffin-NBP2-52952-img0002.jpg)