| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92846-0.02ml | |||
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.​​" alt="MAP2 was detected in immersion fixed paraffin-embedded sections of mouse Hippocampus using Mouse Anti-Human Mouse MAP2, Monoclonal Antibody (Catalog # NBP2-50032) at 1:12,000 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.​​" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50032SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44979 | |||
| Azide and BSA Free | NBP2-34434-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77396SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50052SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47858-0.1mg | |||
| IHC-Prediluted | NBP2-48223 | |||
![Western Blot: Claudin-5 Antibody [NBP2-92846] -analysis of various lysates, using CLDN5 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-92846_rabbit-polyclonal-claudin-5-antibody-western-blot-28112023132836..jpg "Western Blot: Claudin-5 Antibody [NBP2-92846] -analysis of various lysates, using CLDN5 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody [NBP2-92846] - Analysis of U2OS cells using Claudin-5 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Claudin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92846-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Claudin-5 Antibody - Azide and BSA Free [NBP2-92846] - Immunofluorescence analysis of NIH/3T3 cells using Claudin-5 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92846_rabbit-polyclonal-claudin-5-antibody-4220251429069.jpg%3Ft=1706682212)
![Western Blot: Rab7a Antibody (4X3P6) [NBP3-15444] - Western blot analysis of extracts of various cell lines, using Rab7a Rabbit mAb (NBP3-15444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Rab7a-Antibody-4X3P6-Western-Blot-NBP3-15444-img0002.jpg "Western Blot: Rab7a Antibody (4X3P6) [NBP3-15444] - Western blot analysis of extracts of various cell lines, using Rab7a Rabbit mAb (NBP3-15444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Rab7a Antibody (5A10A4) [Rab7a] - Western blot analysis of various lysates using Rab7a Rabbit mAb at 1:55000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-15444_rabbit-rab7a-mab-5a10a4-52202517152713.jpg)
![Immunocytochemistry/ Immunofluorescence: Rab7a Antibody (5A10A4) [Rab7a] - Confocal imaging of NIH/3T3 cells using Rab7a Rabbit mAb followed by a further incubation with ABflo 488-conjugated Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with Cy3 Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15444_rabbit-rab7a-mab-5a10a4-52202517213545.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-5D5-Immunohistochemistry-Paraffin-NBP1-51612-img0015.jpg "Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 mouse mAb with DAB staining.")
![Western Blot: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis using CDH2 mouse mAb against A431 (1), NIH/3T3 (2), Hela (3), C6 (4) and LNCap (5) cell lysate.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-5D5-Western-Blot-NBP1-51612-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (5D5) [NBP1-51612] - Immunofluorescence analysis of A431 cells using CDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-5D5-Immunocytochemistry-Immunofluorescence-NBP1-51612-img0012.jpg)
![Flow Cytometry: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of PC-2 cells using CDH2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-5D5-Flow-Cytometry-NBP1-51612-img0011.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of human Placenta tissues using CDH2 mouse mAb](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-5D5-Immunohistochemistry-Paraffin-NBP1-51612-img0014.jpg)
![Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Western blot analysis of Cytokeratin 14 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Western-Blot-NBP2-67585-img0008.jpg "Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Western blot analysis of Cytokeratin 14 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (red) at 1/400 dilution and Vimentin (green) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0010.jpg)
![Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of Cytokeratin 14 on different lysates using anti-Cytokeratin 14 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Human skin](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Western-Blot-NBP2-67585-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (red) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0009.jpg)
![Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of STAT3 [p Ser727] on NIH-3T3 cell lysates. Lane 1: NIH-3T3 cells, whole cell lysates, 10 ug/lane. Lane 2: NIH-3T3 cells were starved 6h, whole cell lysates, 10 ug/lane. Lane 3/4: NIH-3T3 cells were starved 6h, then treated with 200 ng/ml EGF for 10 min, whole cell lysates, 10 ug/lane. Lane 5: NIH-3T3 cells were starved 6h, then treated with 200 ng/ml EGF for 10 min, and then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10 ug/lane. The primary antibody Anti-STAT3 [p Ser727] (1/500) , Anti-STAT3 antibody ( 1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 88 kDa Observed band size: 88 kDa](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Western-Blot-NBP2-67433-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of STAT3 [p Ser727] on NIH-3T3 cell lysates. Lane 1: NIH-3T3 cells, whole cell lysates, 10 ug/lane. Lane 2: NIH-3T3 cells were starved 6h, whole cell lysates, 10 ug/lane. Lane 3/4: NIH-3T3 cells were starved 6h, then treated with 200 ng/ml EGF for 10 min, whole cell lysates, 10 ug/lane. Lane 5: NIH-3T3 cells were starved 6h, then treated with 200 ng/ml EGF for 10 min, and then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10 ug/lane. The primary antibody Anti-STAT3 [p Ser727] (1/500) , Anti-STAT3 antibody ( 1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 88 kDa Observed band size: 88 kDa")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunocytochemistry-Immunofluorescence-NBP2-67433-img0003.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0014.jpg)
![Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of Phospho-STAT3(S727) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lane 1: NIH/3T3 cell lysate Lane 2: Jurkat cell lysate](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Western-Blot-NBP2-67433-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Human U251 glioma cell line, whole cell lysates.Cells were treated with SP600125, DB07268, JNK-IN-8 and SB203580. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Western-Blot-NBP2-67433-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunocytochemistry-Immunofluorescence-NBP2-67433-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunocytochemistry-Immunofluorescence-NBP2-67433-img0002.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded human lung tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0004.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0005.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0006.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat liver tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0007.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3 (S727) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0011.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0012.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0013.jpg)
![Western blot analysis of Hela cell lysate with NBP2-67433 at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 30 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-67433, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 88.1 kDa Observed band size: 88 kDa](http://images.novusbio.com/fullsize/nbp2-67433_-western-blot-639204586668891819.jpg)
![Western blot analysis of Hela cell lysate with NBP2-67433 at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: C6 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-67433, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 88.1 kDa Observed band size: 88 kDa](http://images.novusbio.com/fullsize/nbp2-67433_-western-blot-639204597490422236.jpg)
![Western blot analysis on different lysates with NBP2-67433 at 1/1,000 dilution. Lane 1: PC-12 cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: C6 treated with 200ng/mL TPA for 35 minutes cell lysate (20 µg/Lane) Lane 4: Rat cerebellum tissue lysate (40 µg/Lane) Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67433 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67433_-western-blot-639204598086887785.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032] - Mixed neuron and glia cultures stained with NBP2-50032 (red) and chicken antibody to Vimentin (green) and DNA (blue). NBP2-50032 reveals strong cytoplasmic staining of dendrites and perikarya of neuronal cells, while Vimentin was visualized in astrocytes and fibroblasts.](http://images.novusbio.com/fullsize/MAP2-Antibody-2C4-Immunocytochemistry-Immunofluorescence-NBP2-50032-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032] - Analysis of adult rat hippocampus section stained with mouse mAb to MAP2A/B/C/D, NBP2-50032, dilution 1:5,000 in green, and chicken pAb to FOX2, dilution 1:2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. NBP2-50032 antibody labels all isotypes of the MAP2 protein which are found in the perikarya and dendrites of neurons. The FOX2 antibody stains the nuclei of a subset of neuronal cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-2C4-Immunocytochemistry-Immunofluorescence-NBP2-50032-img0006.jpg)
![Western Blot: MAP2 Antibody (2C4) [NBP2-50032] - Analysis of tissue and cell lysates using mouse mAb to MAP2C/D, NBP2-50032, dilution 1:5,000 in green, and chicken pAb to Vimentin, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH/3T3 cell lysates. A band at about 280 kDa corresponds to full length intact MAP2A/2B isotypes. The bands that appear at about 70kDa represent MAP2C/D isotypes. Multiple bands in between are likely to be in vivo fragments of MAP2A/B. Only the SH-SY5Y cells, which have neuronal properties express MAP2 protein. Vimentin antibody binds to vimentin protein showing a single band at ~50 kDa, which is present in all preparations.](http://images.novusbio.com/fullsize/MAP2-Antibody-2C4-Western-Blot-NBP2-50032-img0005.jpg)
![Western Blot: p53 Antibody (SPM590) [NBP2-34387] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (SPM590).](http://images.novusbio.com/fullsize/p53-Antibody-SPM590-Western-Blot-NBP2-34387-img0003.jpg "Western Blot: p53 Antibody (SPM590) [NBP2-34387] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (SPM590).")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM590) [NBP2-34387] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with P53 Monoclonal antibody (SPM590)](http://images.novusbio.com/fullsize/p53-Antibody-SPM590-Immunohistochemistry-Paraffin-NBP2-34387-img0002.jpg)
![Western Blot: HSP60 Antibody [NBP1-77396] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-77396-img0007.jpg "Western Blot: HSP60 Antibody [NBP1-77396] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77396] - Hsp60 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77396-img0004.jpg)
![Immunohistochemistry: HSP60 Antibody [NBP1-77396] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunohistochemistry-NBP1-77396-img0006.jpg)
![Flow (Intracellular): HSP60 Antibody [NBP1-77396] - An intracellular stain was performed on HeLa cells with HSP60 Antibody NBP1-77396AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/HSP60-Antibody-Flow-Intracellular-NBP1-77396-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77396] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77396-img0005.jpg)
![Simple Western: HSP60 Antibody [NBP1-77396] - Image shows a specific band for Hsp60 in 0.05 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-Simple-Western-NBP1-77396-img0009.jpg)
![Western Blot: GAP-43 Antibody (5E8) [NBP2-50052] - Analysis of different tissue and cell lysates using mouse mAb to GAP43, NBP2-50052, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at 43 kDa mark correspond to GAP43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.](http://images.novusbio.com/fullsize/GAP-43-Antibody-5E8-Western-Blot-NBP2-50052-img0005.jpg "Western Blot: GAP-43 Antibody (5E8) [NBP2-50052] - Analysis of different tissue and cell lysates using mouse mAb to GAP43, NBP2-50052, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at 43 kDa mark correspond to GAP43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody (5E8) [NBP2-50052] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to GAP43, NBP2-50052, dilution 1:1,000, in red, and costained with chicken pAb to GFAP, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of the neuronal cells, and GFAP antibody stains astroglia.](http://images.novusbio.com/fullsize/GAP-43-Antibody-5E8-Immunocytochemistry-Immunofluorescence-NBP2-50052-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody (5E8) [NBP2-50052] - Mixed rat neuronal cultures stained with NBP2-50052 (green). The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.](http://images.novusbio.com/fullsize/GAP-43-Antibody-5E8-Immunocytochemistry-Immunofluorescence-NBP2-50052-img0003.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0011.jpg "Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F9NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0008.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B in human prostate](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0010.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0007.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis of S100B (15F4BN) in human brain tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in human prostate tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B (15F9NB) in human brain.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0009.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) [Azide Free] [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0002.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-+-6F-H2-Immunohistochemistry-Paraffin-NBP2-44607-img0006.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).")
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded Rat kidney stained with WT1 monoclonal antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/nbp2-44607_mouse-monoclonal-wt1-antibody-wt1-857-6f-h2-1772024100320.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Fetal kidney stained with WT1 Monoclonal Antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/nbp2-44607_mouse-monoclonal-wt1-antibody-wt1-857-6f-h2-177202495943.jpg)
