| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85814-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70653 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70342 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64792SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Atto594 | NBP3-14765-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22125SS | |||
| Azide and BSA Free | NBP2-80752 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25148SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92686SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89388-25ul | |||
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Analysis in human ovary and skeletal muscle tissues. Corresponding VIM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Analysis in human ovary and skeletal muscle tissues. Corresponding VIM RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Simple-Western-NBP1-85814-img0006.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human cervix, uterine shows moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0027.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0024.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0025.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human ovary shows moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0026.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0028.jpg)
![Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Simple-Western-NBP1-85814-img0007.jpg)
![Simple Western: Vimentin Antibody [NBP1-85814] - Electropherogram image(s) of corresponding Simple Western lane view. Vimentin antibody was used at 1:20 dilution on NIH-3T3 lysates(s).](http://images.novusbio.com/fullsize/Vimentin-Antibody-Simple-Western-NBP1-85814-img0008.jpg)
![Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Analysis of extracts (35ug) from 2 different Human cell lines, dilution (1:500).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-OTI4F10-Western-Blot-NBP2-01152-img0010.jpg "Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Analysis of extracts (35ug) from 2 different Human cell lines, dilution (1:500).")
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ErbB2/HER2.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-4F10-Immunocytochemistry-Immunofluorescence-NBP2-01152-img0008.jpg)
![Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Human Kidney tissue using anti-ErbB2/HER2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Immunohistochemistry-Paraffin-NBP2-01152-img0005.jpg)
![Flow Cytometry: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ErbB2/HER2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Flow-Cytometry-NBP2-01152-img0001.jpg)
![Western Blot: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ErbB2/HER2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ErbB2/HER2.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Western-Blot-NBP2-01152-img0002.jpg)
![Western Blot: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - 1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8:Colon.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Western-Blot-NBP2-01152-img0007.jpg)
![Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ErbB2/HER2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Immunohistochemistry-Paraffin-NBP2-01152-img0003.jpg)
![Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ErbB2/HER2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Immunohistochemistry-Paraffin-NBP2-01152-img0004.jpg)
![Western Blot: Catalase Antibody (1B8) [NBP2-00492] Analysis of extracts (35ug) from 9 different cell lines by using anti-Catalase monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Western-Blot-NBP2-00492-img0015.jpg "Western Blot: Catalase Antibody (1B8) [NBP2-00492] Analysis of extracts (35ug) from 9 different cell lines by using anti-Catalase monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Catalase Antibody (OTI1B8) [NBP2-00492] - Mouse hepatocytes stained with catalase antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Catalase-Antibody-OTI1B8-Immunocytochemistry-Immunofluorescence-NBP2-00492-img0019.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human lung tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0010.jpg)
![Flow Cytometry: Catalase Antibody (1B8) [NBP2-00492] - Analysis of Jurkat cells, using anti-Catalase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Flow-Cytometry-NBP2-00492-img0017.jpg)
![Western Blot: Catalase Antibody (1B8) [NBP2-00492] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Catalase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Catalase.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Western-Blot-NBP2-00492-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Catalase Antibody (1B8) [NBP2-00492] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Catalase.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunocytochemistry-Immunofluorescence-NBP2-00492-img0018.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0004.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0006.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human endometrium tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0007.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human liver tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0009.jpg)
![Flow Cytometry: Catalase Antibody (1B8) [NBP2-00492] - Analysis of Hela cells, using anti-Catalase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Flow-Cytometry-NBP2-00492-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.](http://images.novusbio.com/fullsize/F-Actin-Antibody-NH3-Immunocytochemistry-Immunofluorescence-NB100-64792-img0001.jpg "Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Actin Gamma 1 Antibody (NH3) [NB100-64792] - Human inner ear tissue section stained with Actin Gamma 1 antibody at 1:100 overnight at 4C and detected with Alexa Fluor 488 Donkey anti-mouse at 1:200. Nuclei stained with DAPI. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-NH3-Immunohistochemistry-Paraffin-NB100-64792-img0002.jpg)
![Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of Mouse brain, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Western-Blot-NBP3-15842-img0008.jpg "Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of Mouse brain, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of HeLa cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Western-Blot-NBP3-15842-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts from normal (control) and beta-Catenin knockout (KO) 293T cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Knockout-Validated-NBP3-15842-img0001.jpg)
![Immunoprecipitation: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug beta-Catenin antibody (NBP3-15842). Western blot was performed from the immunoprecipitate using beta-Catenin (NBP3-15842) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Immunoprecipitation-NBP3-15842-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517284937.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517184515.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517162378.jpg)
![Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Western blot analysis of lysates from NIH/3T3 cells, using [KO Validated] beta-Catenin Rabbit mAb at 1:1000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517223995.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Western blot analysis of lysates from wild type(WT) and beta-Catenin knockout (KO) 293T(KO) cells, using [KO Validated] beta-Catenin Rabbit mAb at 1:1000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517202968.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-522025171893.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517223986.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human solitary fibrous tumor tissue using [KO Validated] beta-Catenin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517172874.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Human prostate cancer tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-5220251718979.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517273553.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517284993.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Western-Blot-NBP2-54789-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54789-img0004.jpg)
![Immunohistochemistry: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Immunohistochemistry analysis of rat brain injected with Type 1 human alpha synuclein PFFs (NBP2-54789). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 human alpha synuclein PFFs (NBP2-54789) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30-days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the striatum close to an injection site.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-54789-img0007.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to active alpha Synuclein aggregate (NBP2-54789) and active alpha Synuclein monomer (NBP2-54788) alone. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-54789-img0006.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0008.jpg)
![<b>Biological Strategies Validation. </b>Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1) (Catalog No. NBP2-54789). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Product-Image-NBP2-54789-img0010.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0009.jpg)
![Western Blot: Cytokeratin 18 Antibody (C-04) [NB500-306] - Analysis of cytokeratin 18 in HeLa cells with anti-cytokeratin 18 (C-04) biotin.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-C-04-Western-Blot-NB500-306-img0005.jpg "Western Blot: Cytokeratin 18 Antibody (C-04) [NB500-306] - Analysis of cytokeratin 18 in HeLa cells with anti-cytokeratin 18 (C-04) biotin.")
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [NB500-306] - Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-cytokeratin 18 (C-04). Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-C-04-Immunohistochemistry-Paraffin-NB500-306-img0008.jpg)
![Flow Cytometry: Cytokeratin 18 Antibody (C-04) [NB500-306] - Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-cytokeratin 18 (C-04) FITC.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-C-04-Flow-Cytometry-NB500-306-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [NB500-306] - Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-cytokeratin 18 (C-04). Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-C-04-Immunohistochemistry-Paraffin-NB500-306-img0007.jpg)
![Western Blot: VDAC1 Antibody [NB100-695] - Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead).](http://images.novusbio.com/fullsize/VDAC1-Antibody-Western-Blot-NB100-695-img0004.jpg)
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysates.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-2H7-Western-Blot-NBP2-22125-img0003.jpg "Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysates.")
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of paraffin-embedded kidney cancer tissues using Glucose 6 Phosphate Dehydrogenase mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-2H7-Immunohistochemistry-Paraffin-NBP2-22125-img0006.jpg)
![Flow Cytometry: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of Jurkat cells using Glucose 6 Phosphate Dehydrogenase mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-2H7-Flow-Cytometry-NBP2-22125-img0004.jpg)
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mAb against human Glucose 6 Phosphate Dehydrogenase (AA: 275-515) recombinant protein. (Expected MW is 53.1 kDa)](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-2H7-Western-Blot-NBP2-22125-img0002.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of paraffin-embedded breast cancer tissues using Glucose 6 Phosphate Dehydrogenase mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-2H7-Immunohistochemistry-Paraffin-NBP2-22125-img0005.jpg)
![ELISA: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-2H7-ELISA-NBP2-22125-img0001.jpg)
![Simple Western: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125] - B[a]P-Induced Changes in Cell Cycle. (a) Beas-2B cells treated with 1 µM or 10 µM B[a]P (24 h), 3 µg/mL aphidicolin (18 h), & 100 ng/mL nocodazole (18 h) were prepared for cell imaging post-treatment & immunofluorescent staining. A reference cell cycle diagram is shown. The categorization schematic applied to immunofluorescent images is presented as well as a reference diagram of mammalian cell cycle. Scale bar = 25 μm. (b) Beas-2B cells treated with B[a]P were assessed for WST1 metabolism. n = 5. (c) Quantitative EdU uptake among EdU + Beas-2B cells treated with B[a]P. n = 4. (d) Cumulative cell cycle phase distributions of Beas-2B cells treated with B[a]P or cell cycle controls are presented. n = 4, except nocodazole-treated Beas-2B for which n = 2. (e) Magnitude changes compared to untreated controls among each categorical cell cycle phase are presented; bar colors coincide with phase designations presented in (d). n = 4, except nocodazole-treated Beas-2B for which n = 2. Positive changes denote increased percentage of cells in a specific phase among treated cells, whilst negative values denote decreased percentage. Endpoints are the arithmetic mean of listed independent experiments with error bars denoting one standard error of the mean. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37217524), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22125_mouse-monoclonal-glucose-6-phosphate-dehydrogenase-antibody-2h7-310202416114769.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Analysis of lysates from different cell lines probed with mouse mAb to high mobility group protein B1, (HMGB1), NBP2-25148, dilution 1:2,000: [1] protein standard, [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y. The 25kDa band revealed by MCA-1F3 antibody, corresponds to HMGB1 protein.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Western-Blot-NBP2-25148-img0009.jpg "Western Blot: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Analysis of lysates from different cell lines probed with mouse mAb to high mobility group protein B1, (HMGB1), NBP2-25148, dilution 1:2,000: [1] protein standard, [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y. The 25kDa band revealed by MCA-1F3 antibody, corresponds to HMGB1 protein.")
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Analysis of of HeLa cells stained with mouse mAb to HMGB1,NBP2-25148, dilution 1:2,000 in red and costained with rabbit pAb to GAPDH, dilution 1:2,000 in green. MCA-1F3 antibody stains the chromatin binding protein HMGB1, which is localized in the nuclei.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP2-25148-img0010.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HMGB1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Flow-Cytometry-NBP2-25148-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - HeLa cells grown in tissue culture and stained with NBP2-25148 (red), chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25148 reveals strong nuclear staining which overlaps with the DNA stain.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP2-25148-img0008.jpg)
![Simple Western: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Simple Western lane view shows a specific band for HMGB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Simple-Western-NBP2-25148-img0005.jpg)
![Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T and huh-7 lysate.](http://images.novusbio.com/fullsize/EWSR1-Antibody-5H7-Western-Blot-NBP1-92686-img0005.jpg "Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T and huh-7 lysate.")
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (5H7) [NBP1-92686] - HeLa cell cultures stained with antibody NBP1-92686 (green) and chicken antibody to vimentin (NB300-223, red). Blue is a DNA stain, and it is clear that in these cells EWSR1 is localized along with the DNA in the nucleus.](http://images.novusbio.com/fullsize/EWSR1-Antibody-5H7-Immunocytochemistry-Immunofluorescence-NBP1-92686-img0003.jpg)
![Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Analysis of different cell line lysates using EWSR1 antibody, dilution 1:1000 (Green): [1] protein standard (Red), [2] HeLa, [3] HEK293, [4] mouse NIH-3T3, [5] rat PC12, [6] equine NBL6 and [7] canine A72 cells. The strong band at ~80kDa corresponds to the EWS protein seen in all species tested.](http://images.novusbio.com/fullsize/EWSR1-Antibody-5H7-Western-Blot-NBP1-92686-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: S100A6 Antibody [NBP1-89388] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89388-img0018.jpg "Immunocytochemistry/Immunofluorescence: S100A6 Antibody [NBP1-89388] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: S100A6 Antibody [NBP1-89388] - Staining of human urinary bladder shows moderate cytoplasmic and nuclear positivity in urothelial cells.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89388-img0022.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody [NBP1-89388] - Staining of human gastrointestinal shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89388-img0019.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody [NBP1-89388] - Staining of human kidney shows moderate cytoplasmic positivity in cells in distal tubules and in glomeruli.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89388-img0020.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody [NBP1-89388] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89388-img0021.jpg)
