| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-21690-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22191-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56411SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP2-46876 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33076 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34671-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54792SS | |||
| Azide and BSA Free | NBP2-80556 | |||
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Western-Blot-NB600-1164-img0031.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of Protein Disulfide Isomerase/P4HB using monoclonal antibody shows staining in A549 Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunocytochemistry-Immunofluorescence-NB600-1164-img0027.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunohistochemistry-Paraffin-NB600-1164-img0030.jpg)
![Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of 3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Flow-Cytometry-NB600-1164-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Staining of rat epithelial cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunocytochemistry-Immunofluorescence-NB600-1164-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of NS-1 Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunocytochemistry-Immunofluorescence-NB600-1164-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis shows staining in HMVEC Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunocytochemistry-Immunofluorescence-NB600-1164-img0026.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Normal deparaffinized Human tonsil tissue tissues.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunohistochemistry-Paraffin-NB600-1164-img0028.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Both normal and cancer biopsies of deparaffinized Human liver tissues.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunohistochemistry-Paraffin-NB600-1164-img0029.jpg)
![Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of K562 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Flow-Cytometry-NB600-1164-img0022.jpg)
![Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of Hela cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Flow-Cytometry-NB600-1164-img0023.jpg)
![Western Blot: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - analysis of Rat skeletal muscle tissue lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 5A5 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5A5-Western-Blot-NBP1-21690-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - analysis of Rat skeletal muscle tissue lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 5A5 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5A5-Immunocytochemistry-Immunofluorescence-NBP1-21690-img0005.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mouse mAb against Hela (1), HepG2 (2), A549 (3), Jurkat(4), L1210 (5) and Cos7 (6) cell lysate.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Western-Blot-NBP2-22191-img0006.jpg "Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mouse mAb against Hela (1), HepG2 (2), A549 (3), Jurkat(4), L1210 (5) and Cos7 (6) cell lysate.")
![Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using Integrin beta 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-22191-img0003.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Flow cytometric analysis of Hela cells using Integrin beta 1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Flow-Cytometry-NBP2-22191-img0001.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mAb against human ITGB1 (AA: 50-270) recombinant protein. (Expected MW is 50.6 kDa)](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Western-Blot-NBP2-22191-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Integrin beta 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-22191-img0002.jpg)
![ELISA: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-ELISA-NBP2-22191-img0004.jpg)
![CyTOF-ready: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Analysis of Integrin beta 1/CD29 antibody on human tissue. Integrin beta 1/CD29 (green). Scale, 50um. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-CyTOF-ready-NBP2-22191-img0007.jpg)
![Western Blot: pan Actin Antibody (AC-40) [NB120-11003] - Cell line lysates lanes: 1. HeLa, 2. JURKAT, 3. COS7, 4. NIH-3T3, 5. PC-12, 6. RAT2, 7. CHO, 8. MDBK, 9. MDCK.](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Western-Blot-NB120-11003-img0004.jpg "Western Blot: pan Actin Antibody (AC-40) [NB120-11003] - Cell line lysates lanes: 1. HeLa, 2. JURKAT, 3. COS7, 4. NIH-3T3, 5. PC-12, 6. RAT2, 7. CHO, 8. MDBK, 9. MDCK.")
![Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal Anti-Actin produced in mouse clone No. AC-40. The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Immunocytochemistry-Immunofluorescence-NB120-11003-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - conserved C-terminus specific Monoclonal staining of human tongue frozen section with Monoclonal Anti-Actin](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Immunocytochemistry-Immunofluorescence-NB120-11003-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - Rat2 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:250 Monoclonal Anti-Actin Clone: AC-40. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Immunocytochemistry-Immunofluorescence-NB120-11003-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody [NB600-579] - Human skin stained with Cytokeratin antibody.](http://images.novusbio.com/fullsize/pan-Cytokeratin-Antibody-Immunohistochemistry-Paraffin-NB600-579-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody [NB600-579] - Human skin stained with Cytokeratin antibody.")
![Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing somites in stage 46 Xenopus laevis embryo using Actin Alpha 1 Cardiac Muscle Antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Actin-Alpha-Skeletal-Cardiac-Antibody-22D3-Immunofluorescence-NBP1-97725-img0004.jpg "Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing somites in stage 46 Xenopus laevis embryo using Actin Alpha 1 Cardiac Muscle Antibody. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Paraffin section of human cardiac muscle immunostained with 22D3 (1:500)](http://images.novusbio.com/fullsize/Actin-Alpha-Skeletal-Cardiac-Antibody-22D3-Immunohistochemistry-Paraffin-NBP1-97725-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo](http://images.novusbio.com/fullsize/Actin-Alpha-Skeletal-Cardiac-Antibody-22D3-Immunocytochemistry-Immunofluorescence-NBP1-97725-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing myocardium in 1 month old zebrafish embryo](http://images.novusbio.com/fullsize/Actin-Alpha-Skeletal-Cardiac-Antibody-22D3-Immunocytochemistry-Immunofluorescence-NBP1-97725-img0003.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in A) HeLa, B) human liver, C) mouse liver, and D) rat liver lysate using NB100-56411 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were use for this test.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NB100-56411-img0004.jpg "Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in A) HeLa, B) human liver, C) mouse liver, and D) rat liver lysate using NB100-56411 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were use for this test.")
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in formalin-fixed, paraffin-embedded mouse liver tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NB100-56411-img0002.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in mouse liver tissue enriched using Endoplasmic Reticulum Enrichment Kit and 1 ug/ml of NB100-56411 and 0.25 ug/ml of anti-GAPDH loading control. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NB100-56411-img0003.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NB100-56411] - Simple Western lane view shows a specific band for GRP78/HSPA5 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NB100-56411-img0005.jpg)
![Immunohistochemistry: Collagen II Antibody [NBP1-77795] - Staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.](http://images.novusbio.com/fullsize/Collagen-II-Antibody-Immunohistochemistry-NBP1-77795-img0005.jpg "Immunohistochemistry: Collagen II Antibody [NBP1-77795] - Staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.")
![Immunohistochemistry: Collagen II Antibody [NBP1-77795] - Histological analysis and the expression of PIEZO1 (NBP1-78537) and collage II (NBP1-77795) in human nucleus pulposus tissues. A, B Hematoxylin and eosin (H&E) staining in human NP tissues of the control or the IVDD group (200×). C, D PIEZO1 immunostaining in human NP tissues of the control or the IVDD group (100×). E The percentage of PIEZO1 positive cells to all cells in the field (%). F, G Collagen II immunostaining in human NP tissues of the control or the IVDD group (100×). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35606793/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-77795_rabbit-polyclonal-collagen-ii-antibody-2552023153957.jpg)
![Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 cells. PPI negative cell population was gated for analysis.](http://images.novusbio.com/fullsize/CD34-Antibody-ICO-115-Flow-Cytometry-NBP2-29455-img0002.jpg "Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 cells. PPI negative cell population was gated for analysis.")
![Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/CD34-Antibody-ICO-115-Flow-Cytometry-NBP2-29455-img0001.jpg)
![Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Simple-Western-NBP2-32867-img0002.jpg "Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: HSP60 Antibody (LK2) [NBP2-32867] - Western blot analysis of Hep2 cell lysate using HSP60 Mouse Monoclonal Antibody (LK2).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Western-Blot-NBP2-32867-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK2) [NBP2-32867] - Immunofluorescent staining of MEOH-fixed HeLa cells using followed by goat anti-Mouse labeled with CF488 (Green); Nuclei are labeled with NucSpot red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Immunocytochemistry-Immunofluorescence-NBP2-32867-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK2) [NBP2-32867] - Formalin-fixed, paraffin-embedded human liver in colon stained with HSP60 Mouse Monoclonal Antibody (LK2).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Immunohistochemistry-Paraffin-NBP2-32867-img0008.jpg)
![Western Blot (Negative): HSP60 Antibody (LK2) [NBP2-32867] - Immunoblot analysis of whole bacterial lysate of Mtb cells pretreated with (+) or without (-) proteinase K followed by incubation with K632-7 poly-Ub chains at 4C for 4 hr. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-09955-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Western-Blot-Negative-NBP2-32867-img0006.jpg)
![Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Electropherogram image of the corresponding Simple Western lane. HSP60 antibody was used at 10 ug/ml dilution of HeLa and HepG2 lysates(s) respectively.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Simple-Western-NBP2-32867-img0004.jpg)
![Western Blot: HSP60 Antibody (LK2) [NBP2-32867] - Mtb Rv1468c directly binds to poly-Ub chains in a UBA-dependent manner. a, b Immunoblot analysis of whole bacterial lysate of Mtb cells pretreated w/ (+) or w/out (–) proteinase K followed by incubation w/ K632–7 (a) or K482–7 (b) poly-Ub chains at 4 °C for 4 h. c Confocal microscopy analysis for colocalization of pSC301-Mtb stably expressing green fluorescent protein (GFP) w/ K632–7 or K482–7 Ub chains in vitro. Bacteria (green) treated w/ (+) or w/out (–) proteinase K further incubated w/ poly-Ub chains as in a & b, & then immunostained using anti-Ub antibody (red). Scale bars, 10 μm. The % colocalizations of Ub & Mtb shown at the bottom. A total of 100 bacterial cells counted. Two-tailed unpaired t-test performed. d Schematic representation of Mtb Rv1468c constructs used. e In vitro interaction analysis of Ub & GST-tagged full-length Mtb Rv1468c or the truncated mutants as indicated in d. Purified recombinant GST-tagged Mtb Rv1468c variants immobilized on glutathione sepharose resin. Mono-Ub (His6-tagged), K63 or K48-linked poly-Ub (Ub2–7) added, & then the protein complexes assayed by immunoblotting w/ indicated antibodies. f Immunoblot analysis of proteins immunoprecipitated (IP) w/ anti-Flag M2 Affinity Gel from lysates of HEK293T cells transfected w/ empty vector (–) or hemagglutinin (HA)-tagged Ub (+) & Flag-tagged wild-type (WT) Rv1468c or its mutants (V64G or L65G). g Confocal microscopy analysis of HeLa cells cotransfected w/ vectors encoding HA-tagged Ub (red) & GFP-tagged WT Rv1468c, V64G or L65G mutant (green). Nuclei (blue) stained w/ DNA-binding dye DAPI. Scale bars, 10 μm. Results representatives from at least 3 independent experiments (mean ± s.e.m. of n = 5 in c). The source data used in c provided in Source Data Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31036822), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32867_mouse-monoclonal-hsp60-antibody-lk2-31020241612249.jpg)
![Western Blot: OCT4 Antibody (486) [NBP2-15052] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Western-Blot-NBP2-15052-img0013.jpg "Western Blot: OCT4 Antibody (486) [NBP2-15052] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Paraffin-embedded human cervical carcinoma.Oct4 stained by Oct4 antibody [GT486] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunohistochemistry-Paraffin-NBP2-15052-img0016.jpg)
![Flow Cytometry: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody dilution: 1:50](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Flow-Cytometry-NBP2-15052-img0009.jpg)
![Western Blot: OCT4 Antibody (486) [NBP2-15052] - Sample (10 ug of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant OCT3/4 protein 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Western-Blot-NBP2-15052-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cell were fixed in 4% Paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunocytochemistry-Immunofluorescence-NBP2-15052-img0008.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - BT483 xenograft, using OCT3/4 antibody at 1:200 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunohistochemistry-Paraffin-NBP2-15052-img0001.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Imaging of Rabbit stem cels in Bone marrow of tibia. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunohistochemistry-Paraffin-NBP2-15052-img0012.jpg)
![Immunoprecipitation: OCT4 Antibody (486) [NBP2-15052] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune mouse IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunoprecipitation-NBP2-15052-img0010.jpg)
![Western Blot: OCT4 Antibody (486) [NBP2-15052] - Mouse embryonic stem cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (NBP2-15052) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-910202420134071.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-91020242011495.jpg)
![Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-9102024202468.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: OCT4 Antibody (486) [NBP2-15052] - Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-1610202419405748.jpg)
![Oct4 antibody [GT486] detects Oct4 protein by western blot analysis.A. 30 ug human ESC whole cell lysate/extract10% SDS-PAGE (NBP2-15052) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-western-blot-2611202518301214.jpg)
![Western Blot: ABCA1 Antibody (1276B) [NBP2-54792] - Mouse Raw264.7 and human THP-1 cells were treated with and without the LXR agonist TO901317 (1 uM) for 24 hours. Total protein was then separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA1 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Western-Blot-NBP2-54792-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ABCA1 Antibody (1276B) [NBP2-54792] - Mouse Raw264.7 and human THP-1 cells were treated with and without the LXR agonist TO901317 (1 uM) for 24 hours. Total protein was then separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA1 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) [NBP2-54792] - Analysis of a FFPE tissue section of mouse liver using ABCA1 antibody (1276B) Lot ckjv02 at 1:100 dilution. The primary antibody bound to ABCA1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This ABCA1 antibody generated specific staining in the membranes of the hepatocytes and the Kupffer cells. As expected for ABCA1, the staining signal was stronger in the Kupffer cells which are the tissue macrophages in liver.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunohistochemistry-Paraffin-NBP2-54792-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ABCA1 Antibody (1276B) [NBP2-54792] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ABCA1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunocytochemistry-Immunofluorescence-NBP2-54792-img0003.jpg)
![Flow (Intracellular): ABCA1 Antibody (1276B) [NBP2-54792] - An intracellular stain was performed on HepG2 Cells with ABCA1 (1276B) antibody NBP2-54792 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Flow-Intracellular-NBP2-54792-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ABCA1 Antibody (1276B) [NBP2-54792] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ABCA1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunocytochemistry-Immunofluorescence-NBP2-54792-img0002.jpg)
![Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) [NBP2-54792] - Analysis of a FFPE tissue section of mouse liver using ABCA1 antibody (1276B) Lot ckjv0116091 at 1:50 dilution. The primary antibody bound to ABCA1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This ABCA1 antibody generated specific staining in the membranes of the hepatocytes and the Kupffer cells. As expected for ABCA1, the staining signal was stronger in the Kupffer cells which are the tissue macrophages in liver.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunohistochemistry-Paraffin-NBP2-54792-img0005.jpg)