| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73253 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71662SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23602SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38231-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92821-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82883-25ul | |||
![Western Blot: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Patched 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Patched 1.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Western-Blot-NBP1-47945-img0023.jpg "Western Blot: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Patched 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Patched 1.")
![Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody (OTI5C7) [NBP1-47945] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Patched 1.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunocytochemistry-NBP1-47945-img0013.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Human endometrium tissue.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0024.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0014.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0015.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0016.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0017.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0018.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0019.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Patched 1 Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0020.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Human Kidney tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0021.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Human pancreas tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0022.jpg)
![Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched 1-PTCH Antibody - BSA Free-Western Blot-NB200-118-img0004.jpg "Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Evaluation of IGF-1, IGF1R and HH pathway expression in fibroblasts and oral squamous carcinoma cells. IGF1 mRNA expression in SCC-4 and MF1 cells, evaluated by RT-qPCR. (C) Evaluation of HH pathway component expression in MF1 by immunocytochemistry. Patched 1/PTCH Antibody (NB200-118); Sonic Hedgehog/Shh Antibody (NBP2-22126). Magnification: 400x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB200-118-img0003.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Increased Ihh mRNA and protein levels and activity in Smad2 and Smad3 mutant growth plates.All images are P0 proximal tibias. Immunostaining for Ptch1, showing increased protein levels throughout the growth plate in mutants, particularly in the resting zone. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006352) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Immunohistochemistry-NB200-118-img0002.jpg)
![Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Analysis of Human PTCH1 in Fetal brain Lysate with anti huPTCH1 at a 1:500 dilution.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Western-Blot-NB200-118-img0001.jpg)
![Western Blot: Patched 1/PTCH Antibody [NBP1-71662] - Analysis of Patched 1 in human skeletal muscle protein.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Western-Blot-NBP1-71662-img0006.jpg "Western Blot: Patched 1/PTCH Antibody [NBP1-71662] - Analysis of Patched 1 in human skeletal muscle protein.")
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NBP1-71662] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse lung using 1:50 dilution of PTCH antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane-cytoplasmic staining in bronchiolar and alveolar epithelial cells with stronger signal towards the membranes of the cells.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Immunohistochemistry-Paraffin-NBP1-71662-img0005.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NBP1-71662] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse lung using 1:50 dilution of PTCH antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane-cytoplasmic staining in bronchiolar and alveolar epithelial cells with stronger signal towards the membranes of the cells.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Immunohistochemistry-Paraffin-NBP1-71662-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23602-img0007.jpg "Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: GLI-2 Antibody [NBP2-23602] - GLI-2 (red) and DAPI (blue) in mouse BT-474 primary tumors (breast cancer tissue). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-Paraffin-NBP2-23602-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - GLI-2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:50 was used. Image objective 40x.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23602-img0004.jpg)
![Immunohistochemistry: GLI-2 Antibody [NBP2-23602] - Analysis of GLI-2 in mouse heart.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-NBP2-23602-img0005.jpg)
![Western Blot: RAB8A Antibody (3G1) [H00004218-M02] - RAB8A monoclonal antibody (M02), clone 3G1 Analysis of RAB8A expression in Hela S3 NE.](http://images.novusbio.com/fullsize/RAB8A-Antibody-3G1-Western-Blot-H00004218-M02-img0003.jpg "Western Blot: RAB8A Antibody (3G1) [H00004218-M02] - RAB8A monoclonal antibody (M02), clone 3G1 Analysis of RAB8A expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: RAB8A Antibody (3G1) [H00004218-M02] - Neuromast hair cells of zebrafish embryos stained with RAB8A antibody. Donkey anti-mouse Alexa Fluor 594 secondary antibody was used. Image from verified customer.](http://images.novusbio.com/fullsize/RAB8A-Antibody-3G1-Immunocytochemistry-Immunofluorescence-H00004218-M02-img0005.jpg)
![ELISA: RAB8A Antibody (3G1) [H00004218-M02] - Detection limit for recombinant GST tagged RAB8A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RAB8A-Antibody-3G1-ELISA-H00004218-M02-img0006.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Analysis in human adrenal gland and skin tissues using NBP1-87498 antibody. Corresponding FLOT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-2472024125914..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Analysis in human adrenal gland and skin tissues using NBP1-87498 antibody. Corresponding FLOT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Flotillin-1 Antibody [NBP1-87498] - Analysis in human cell lines SK-MEL-30 and MCF-7 using Anti-FLOT1 antibody. Corresponding FLOT1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-87498-img0012.jpg)
![Western Blot: Flotillin-1 Antibody [NBP1-87498] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-87498-img0009.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunohistochemistry-NBP1-87498-img0013.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Staining of human Adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-247202413032..jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human Fallopian tube shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-247202413122..jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human Stomach shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-247202413216..jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Staining of human Skin shows very weak membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-24720241338..jpg)
![Western Blot: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Patched 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Patched 1.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-OTI5C7-Azide-and-BSA-Free-Western-Blot-NBP2-73253-img0001.jpg "Western Blot: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Patched 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Patched 1.")
![Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Patched 1.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-OTI5C7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73253-img0002.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - Staining of paraffin-embedded Human Kidney tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-OTI5C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73253-img0011.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - Human endometrium tissue.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-OTI5C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73253-img0003.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - Patched 1 Antibody (5c7) [NBP1-47945](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-OTI5C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73253-img0004.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-OTI5C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73253-img0005.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-OTI5C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73253-img0006.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-OTI5C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73253-img0007.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-OTI5C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73253-img0008.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-OTI5C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73253-img0009.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-OTI5C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73253-img0010.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody (OTI5C7) - Azide and BSA Free [NBP2-73253] - Staining of paraffin-embedded Human pancreas tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-OTI5C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73253-img0012.jpg)
![Western Blot: IFT88 Antibody [NB100-2475] - Staining of A549 (A) cell lysate and Mouse Testes (B) lysate (35 ug protein in RIPA buffer). Antibody at 3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/IFT88-Antibody-Western-Blot-NB100-2475-img0008.jpg "Western Blot: IFT88 Antibody [NB100-2475] - Staining of A549 (A) cell lysate and Mouse Testes (B) lysate (35 ug protein in RIPA buffer). Antibody at 3 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: IFT88 Antibody [NB100-2475] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/IFT88-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2475-img0009.jpg)
![Flow Cytometry: IFT88 Antibody [NB100-2475] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/IFT88-Antibody-Flow-Cytometry-NB100-2475-img0007.jpg)
![Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western blot analysis of various lysates, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-101220241252844.jpg "Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western blot analysis of various lysates, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunoprecipitation: Flotillin-1 Antibody [NBP3-38231] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug Flotillin-1 antibody. Western blot was performed from the immunoprecipitate using Flotillin-1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-1012202411545540.jpg)
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-38231] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-10122024121097.jpg)
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-38231] - Immunohistochemistry analysis of paraffin-embedded Rat heart using [KO Validated] Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-101220241252843.jpg)
![Immunocytochemistry/ Immunofluorescence: Flotillin-1 Antibody [NBP3-38231] - Immunofluorescence analysis of NIH/3T3 cells using Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-10122024121062.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western Blot analysis of lysates from wild type (WT) and Flotillin-1 knockout (KO) 293T cells, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-311202516593618.jpg)
![Western Blot: Flotillin-1 Antibody [NB100-1043] - Staining of K562 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by Chemiluminescence.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NB100-1043-img0001.jpg "Western Blot: Flotillin-1 Antibody [NB100-1043] - Staining of K562 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by Chemiluminescence.")
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunohistochemistry-Paraffin-NB100-1043-img0003.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunohistochemistry-Paraffin-NB100-1043-img0002.jpg)
![Western Blot: B9D1 Antibody [NBP2-92821] - Analysis of extracts of various cell lines, using B9D1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/B9D1-Antibody-Western-Blot-NBP2-92821-img0004.jpg "Western Blot: B9D1 Antibody [NBP2-92821] - Analysis of extracts of various cell lines, using B9D1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: B9D1 Antibody [NBP2-92821] - Immunofluorescence analysis of U2OS cells using B9D1 Rabbit pAb (NBP2-92821) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/B9D1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92821-img0006.jpg)
![Immunohistochemistry-Paraffin: B9D1 Antibody [NBP2-92821] - Paraffin-embedded rat kidney using B9D1 .](http://images.novusbio.com/fullsize/B9D1-Antibody-Immunohistochemistry-Paraffin-NBP2-92821-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: B9D1 Antibody [NBP2-92821] - Immunofluorescence analysis of L929 cells using B9D1 Rabbit pAb (NBP2-92821) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/B9D1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92821-img0005.jpg)
![Immunohistochemistry-Paraffin: B9D1 Antibody [NBP2-92821] - Paraffin-embedded human stomach using B9D1 .](http://images.novusbio.com/fullsize/B9D1-Antibody-Immunohistochemistry-Paraffin-NBP2-92821-img0001.jpg)
![Immunohistochemistry-Paraffin: B9D1 Antibody [NBP2-92821] - Paraffin-embedded mouse lung using B9D1 .](http://images.novusbio.com/fullsize/B9D1-Antibody-Immunohistochemistry-Paraffin-NBP2-92821-img0002.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Analysis in human testis and skin tissues using antibody. Corresponding NEK1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82883-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Analysis in human testis and skin tissues using antibody. Corresponding NEK1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Staining of human endometrium, fallopian tube, skin and testis using Anti-NEK1 antibody NBP1-82883 (A) shows similar protein distribution across tissues to independent antibody NBP1-82884 (B).](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82883-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: NEK1 Antibody [NBP1-82883] - Staining of human cell line U-251 MG shows localization to nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82883-img0006.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Staining of human Fallopian tube shows strong cytoplasmic/ membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82883-img0007.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Staining of human endometrium shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82883-img0008.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82883-img0009.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Staining of human skin shows weak cytoplasmic positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82883-img0010.jpg)