| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1596SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60011SS | |||
| Azide and BSA Free | NBP2-81012 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47858-0.1mg | |||
| IHC-Prediluted | NBP2-48223 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88706-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18564-25ul | |||
| Azide and BSA Free | NBP3-44379 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18547-25ul | |||
| Azide and BSA Free | NBP3-44378 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74052 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87861-25ul | |||
![Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Western-Blot-NB100-1596-img0005.jpg "Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1596-img0008.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 200 um (1st image) and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0006.jpg)
![Western Blot: Aromatase Antibody [NB100-1596] - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure, 1-2 minutes.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Western-Blot-NB100-1596-img0003.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of hypothyroid (Hypo; solid bar; n = 6) rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of hypothyroid ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 100 um (1st image); and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0007.jpg)
![Simple Western: Aromatase Antibody [NB100-1596] - Aromatase expression by Western blotting (WES system) from in vitro activated M1 and M2 rat macrophages using Aromatase antibody. Controls include ovary (positive control) and ovary in which the primary antibody was preincubated with an aromatase blocking peptide for 1 h, as well as KGN cells with (positive control) and without (negative control) forskolin treatment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29898754/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase Antibody-Simple Western-NB100-1596-img0009.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-31020241539251.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415392577.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415363733.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415293319.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415382447.jpg)
![Simple Western: Aromatase Antibody [NB100-1596] - Metformin decreases aromatase-positive, tumor-associated macrophages. a Quantification of aromatase-positive cells & b CD68-positive (CD68+) macrophages costaining for aromatase in the tumor border of tissues from control or metformin-treated rats. c Representative control & metformin-treated IHC images of CD68 & aromatase dual staining in tissue bordering mammary tumors (T) (arrows: yellow = CD68+aromatase+, brown = CD68+; scale bar = 50um). d Aromatase expression by Western blotting (WES system) from in vitro activated M1 & M2 rat macrophages using two different primary antibodies (Ab #1: Novus NB100-1596; Ab #2: clone 677, Baylor College of Medicine). Controls include ovary (positive control) & ovary in which the primary antibody was preincubated with an aromatase blocking peptide for 1 h, as well as KGN cells with (positive control) & without (negative control) forskolin treatment. e IHC intensity score for ER & PR (0 = no stain; 1 = weak; 2 = moderate; 3 = strong staining). *p < 0.05 Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-018-0974-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-31020241682139.jpg)
![Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Hypomethylation of the Intron 1 CpG island results in WT1 expression. RNA was isolated from K562 and U937 cells growing under atmospheric conditions (Normox) and from U937 cells growing in 1% O2 (Hypox) and was analyzed by RT-PCR using primers that span exon 5 of the WT1 mRNA. Ribosomal RNA 36B4 is used as a positive control, and molecular weight markers (MW) are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Western-Blot-NB110-60011-img0007.jpg "Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Hypomethylation of the Intron 1 CpG island results in WT1 expression. RNA was isolated from K562 and U937 cells growing under atmospheric conditions (Normox) and from U937 cells growing in 1% O2 (Hypox) and was analyzed by RT-PCR using primers that span exon 5 of the WT1 mRNA. Ribosomal RNA 36B4 is used as a positive control, and molecular weight markers (MW) are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: WT1 Antibody (6F-H2) [NB110-60011] - Staining of human kidney glomerulus tissue. Heat mediated antigen retrieval was performed by heating in citrate buffer (pH 6) at 95C for 20 minutes. Image from verified customer review.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Immunohistochemistry-Paraffin-NB110-60011-img0006.jpg)
![Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Whole cell protein from human K562, HEK293 and kidney tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WT1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Western-Blot-NB110-60011-img0005.jpg)
![Immunohistochemistry: WT1 Antibody (6F-H2) [NB110-60011] - Analysis of Wilms Tumor 1 in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Immunohistochemistry-NB110-60011-img0003.jpg)
![Simple Western: WT1 Antibody (6F-H2) [NB110-60011] - Lane view shows a specific band for WT1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Simple-Western-NB110-60011-img0004.jpg)
![Western Blot: WT1 Antibody (6F-H2) - BSA Free [NB110-60011] - Hypomethylation of Intron 1 CpG island results in WT1 expression. (C) Cell lysates from K562, U937, & HL60 cells treated with CoCl2, 5-azacytidine, or untreated controls analyzed by WB using an antibody against WT1. Histone H3 used as a loading control. Size of molecular weight markers is in kDa. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-60011_mouse-monoclonal-wt1-antibody-6f-h2-310202415304296.jpg)
![Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Western-Blot-NBP2-67587-img0008.jpg "Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human testis tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0010.jpg)
![Flow Cytometry: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Flow-Cytometry-NBP2-67587-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of paraffin-embedded mouse kidney tissue using anti-Wilms Tumor Protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0007.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0009.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-+-6F-H2-Immunohistochemistry-Paraffin-NBP2-44607-img0006.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).")
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded Rat kidney stained with WT1 monoclonal antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/nbp2-44607_mouse-monoclonal-wt1-antibody-wt1-857-6f-h2-1772024100320.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Fetal kidney stained with WT1 Monoclonal Antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/nbp2-44607_mouse-monoclonal-wt1-antibody-wt1-857-6f-h2-177202495943.jpg)
![Immunocytochemistry/Immunofluorescence: SON Antibody [NBP1-88706] - Staining of human cell line U-251 MG shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SON-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88706-img0006.jpg "Immunocytochemistry/Immunofluorescence: SON Antibody [NBP1-88706] - Staining of human cell line U-251 MG shows localization to nuclear speckles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human prostate shows moderate to strong positivity in nuclear speckles in glandular cells.](http://images.novusbio.com/fullsize/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0010.jpg)
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human cerebellum shows moderate to strong positivity in nuclear speckles in Purkinje cells.](http://images.novusbio.com/fullsize/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0008.jpg)
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human small intestine shows moderate to strong positivity in nuclear speckles in glandular cells.](http://images.novusbio.com/fullsize/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0009.jpg)
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human tonsil shows moderate to strong positivity in nuclear speckles in germinal center cells.](http://images.novusbio.com/fullsize/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0011.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Analysis in human fallopian tube and lymph node tissues using NBP3-18564 antibody. Corresponding WT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0002.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Analysis in human fallopian tube and lymph node tissues using NBP3-18564 antibody. Corresponding WT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: WT1 Antibody (CL13165) [NBP3-18564] - Analysis in human cell line HEK 293 and human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Western-Blot-NBP3-18564-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (CL13165) [NBP3-18564] - Staining of CACO-2 cells using the Anti-WT1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunocytochemistry-Immunofluorescence-NBP3-18564-img0001.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human lymph node shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0009.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0003.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human ovarian cancer (endometrioid carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0004.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human ovarian cancer (papillary carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0007.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0008.jpg)
![Western Blot: WT1 Antibody (CL13170) [NBP3-18547] - Analysis in human cell line HEK 293 and human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Western-Blot-NBP3-18547-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: WT1 Antibody (CL13170) [NBP3-18547] - Analysis in human cell line HEK 293 and human cell line SK-MEL-30.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: WT1 Antibody (CL13170) [NBP3-18547] - Staining of CACO-2 cells using the Anti-WT1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunocytochemistry-Immunofluorescence-NBP3-18547-img0002.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0018.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Analysis in human testis and prostate tissues using NBP3-18547 antibody. Corresponding WT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0004.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human ovarian cancer (endometrioid carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0012.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0013.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human ovarian cancer (papillary carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0014.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0015.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0016.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0017.jpg)
![Western Blot: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Secernin 1.](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Western-Blot-NBP2-45727-img0010.jpg "Western Blot: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Secernin 1.")
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0009.jpg)
![Western Blot: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Western-Blot-NBP2-45727-img0011.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0001.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0002.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0003.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0004.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0005.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0006.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0007.jpg)
![Immunohistochemistry: Secernin-1 Antibody (1F2) [NBP2-45727] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Secernin-1-Antibody-1F2-Immunohistochemistry-NBP2-45727-img0008.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/WT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31314-img0008.jpg "Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse liver. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/WT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31314-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Rat testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/WT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31314-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Rat testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/WT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31314-img0007.jpg)
![Western Blot: WT1 Antibody [NBP1-31314] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with wt1a + wt1b antibody (NBP1-31314) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-31314_rabbit-polyclonal-wt1-antibody-91020242014636.jpg)
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of extracts of Rat testis, using WT1 antibody (NBP3-16135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/WT1-Antibody-6L9X6-Western-Blot-NBP3-16135-img0006.jpg "Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of extracts of Rat testis, using WT1 antibody (NBP3-16135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of lysates from Mouse testis using WT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-5220251242594.jpg)
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of various lysates using WT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-522025125482.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (6L9X6) [NBP3-16135] - Human Ovarian Serous Carcinoma tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-immunohistochemistry-paraffin-2882024111235..jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (6L9X6) [NBP3-16135] - Human kidney tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-immunohistochemistry-paraffin-2882024111344..jpg)
![Immunohistochemistry: WT1 Antibody (6L9X6) [NBP3-16135] - Immunohistochemistry analysis of paraffin-embedded Human testis tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-52202511361241.jpg)
![Immunocytochemistry/Immunofluorescence: Secernin-1 Antibody [NBP1-87861] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/Secernin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87861-img0008.jpg "Immunocytochemistry/Immunofluorescence: Secernin-1 Antibody [NBP1-87861] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Secernin-1 Antibody [NBP1-87861] - Staining in human cerebral cortex and skeletal muscle tissues using anti-SCRN1 antibody. Corresponding SCRN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Secernin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87861-img0011.jpg)
![Immunohistochemistry-Paraffin: Secernin-1 Antibody [NBP1-87861] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Secernin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87861-img0009.jpg)
![Immunohistochemistry-Paraffin: Secernin-1 Antibody [NBP1-87861] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Secernin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87861-img0010.jpg)
![Western Blot: Aromatase Antibody (5C5N7) [NBP3-15438] - Western blot analysis of extracts of U-87MG cells, using Aromatase antibody (NBP3-15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Western-Blot-NBP3-15438-img0003.jpg "Western Blot: Aromatase Antibody (5C5N7) [NBP3-15438] - Western blot analysis of extracts of U-87MG cells, using Aromatase antibody (NBP3-15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry: Aromatase Antibody (5C5N7) [NBP3-15438] - Immunofluorescence analysis of human placenta using Aromatase Rabbit mAb (NBP3-15438) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Immunohistochemistry-NBP3-15438-img0002.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody (5C5N7) [NBP3-15438] - Human colon using Aromatase Rabbit mAb (NBP3-15438) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Immunohistochemistry-Paraffin-NBP3-15438-img0001.jpg)
![Immunohistochemistry: Aromatase Antibody (5C5N7) [Aromatase] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Aromatase Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15438_rabbit-aromatase-mab-5c5n7-52202517273588.jpg)
![Immunohistochemistry: Aromatase Antibody (5C5N7) [Aromatase] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Aromatase Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15438_rabbit-aromatase-mab-5c5n7-5220251723570.jpg)