| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-109SS | |||
| Azide and BSA Free | NBP2-80882 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85729-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-91662SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44973 | |||
| Azide and BSA Free | NBP2-34621-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24702SS | |||
| Azide and BSA Free | NBP2-33256 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59677SS | |||
| Azide and BSA Free | NBP2-80856 | |||
![Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in IMR32 whole cell lysate.](http://images.novusbio.com/fullsize/n-Myc-Antibody-NMYC-1-Western-Blot-NB200-109-img0002.jpg "Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in IMR32 whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: n-Myc Antibody (NMYC-1) [NB200-109] - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/n-Myc-Antibody-NMYC-1-Immunocytochemistry-Immunofluorescence-NB200-109-img0003.jpg)
![Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in human heart tissue.](http://images.novusbio.com/fullsize/n-Myc-Antibody-NMYC-1-Western-Blot-NB200-109-img0001.jpg)
![Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929] - Human breast carcinoma. IGF1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/IGF-I-Antibody-Immunohistochemistry-Paraffin-NBP2-16929-img0003.jpg "Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929] - Human breast carcinoma. IGF1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Calpain S1 Antibody (3C4) [H00000826-M01] - Analysis of Calpain S1 expression in transfected 293T cell line by Calpain S1 monoclonal antibody (M01), clone 3C4.Lane 1: Calpain S1 transfected lysate(28.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-3C4-Western-Blot-H00000826-M01-img0016.jpg "Western Blot: Calpain S1 Antibody (3C4) [H00000826-M01] - Analysis of Calpain S1 expression in transfected 293T cell line by Calpain S1 monoclonal antibody (M01), clone 3C4.Lane 1: Calpain S1 transfected lysate(28.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Calpain S1 Antibody (3C4) [H00000826-M01] - Analysis of monoclonal antibody to Calpain S1 on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-3C4-Immunocytochemistry-Immunofluorescence-H00000826-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: Calpain S1 Antibody (3C4) [H00000826-M01] - Analysis of monoclonal antibody to Calpain S1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-3C4-Immunohistochemistry-Paraffin-H00000826-M01-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Calpain S1 Antibody (3C4) [H00000826-M01] - Analysis of Calpain S1 over-expressed 293 cell line, cotransfected with Calpain S1 Validated Chimera RNAi ( Cat # H00000826-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with Calpain S1 monoclonal antibody (M01), clone 3C4 (Cat # H00000826-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-3C4-Western-Blot-H00000826-M01-img0012.jpg)
![Western Blot: Calpain S1 Antibody (3C4) [H00000826-M01] - Calpain S1 monoclonal antibody (M01), clone 3C4. Analysis of Calpain S1 expression in PC-12.](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-3C4-Western-Blot-H00000826-M01-img0014.jpg)
![Western Blot: Calpain S1 Antibody (3C4) [H00000826-M01] - Calpain S1 monoclonal antibody (M01), clone 3C4 Analysis of Calpain S1 expression in A-431.](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-3C4-Western-Blot-H00000826-M01-img0015.jpg)
![Sandwich ELISA: Calpain S1 Antibody (3C4) [H00000826-M01] - Detection limit for recombinant GST tagged Calpain S1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-3C4-Sandwich-ELISA-H00000826-M01-img0013.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues . Corresponding CDK1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues . Corresponding CDK1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85729-img0010.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0011.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0012.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0013.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0014.jpg)
![Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.](http://images.novusbio.com/fullsize/APC-Antibody-Western-Blot-NB100-91662-img0004.jpg "Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.")
![Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/APC-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91662-img0006.jpg)
![Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.](http://images.novusbio.com/fullsize/APC-Antibody-Immunohistochemistry-Paraffin-NB100-91662-img0005.jpg)
![Flow Cytometry: APC Antibody [NB100-91662] - An intracellular stain was performed on HeLa cells with APC Antibody NB100-91662AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/APC-Antibody-Flow-Cytometry-NB100-91662-img0007.jpg)
![Western Blot: APC Antibody [NB100-91662] - Extracts from COLO205 cells.](http://images.novusbio.com/fullsize/APC-Antibody-Western-Blot-NB100-91662-img0001.jpg)
![Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - Paraffin-embedded human colon carcinoma tissue.](http://images.novusbio.com/fullsize/APC-Antibody-Immunohistochemistry-Paraffin-NB100-91662-img0003.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Western-Blot-NB100-594-img0015.jpg "Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Immunohistochemistry-Paraffin-NB100-594-img0016.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBEF antibody used for IP at 6 ug per reaction. PBEF was also immunoprecipitated by a previous lot of this antibody and rabbit anti-Visfatin antibody. For blotting immunoprecipitated PBEF, Antibody was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Western-Blot-NB100-594-img0014.jpg)
![Western Blot: KAT3B/p300 Antibody [NB500-161] - Effect of PRDX2 and PRDX4 on HIF-1alpha-p300 interaction. HeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, and exposed to 1% O2 for 24 h. WCL was subject to IP with anti-p300 antibody, followed by immunoblot assays using antibodies against HIF-1alpha, V5, and p300. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/7142), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0012.jpg "Western Blot: KAT3B/p300 Antibody [NB500-161] - Effect of PRDX2 and PRDX4 on HIF-1alpha-p300 interaction. HeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, and exposed to 1% O2 for 24 h. WCL was subject to IP with anti-p300 antibody, followed by immunoblot assays using antibodies against HIF-1alpha, V5, and p300. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/7142), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- p300 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Immunohistochemistry-Paraffin-NB500-161-img0008.jpg)
![Western Blot: KAT3B/p300 Antibody [NB500-161] - Analysis of HeLa lysates using NB500-161. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: KAT3B/p300 Antibody [NB500-161] - Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody NB500-161 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0010.jpg)
![KAT3B/p300 Antibody [NB500-161] - Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p300 antibody NB500-161 used for IP at 6 ug/mg lysate. p300 was also immunoprecipitated by a previous lot (lot NB500-161-1) of this antibody. For blotting immunoprecipitated p300, NB500-161 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-NB500-161-img0011.jpg)
![Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0046.jpg "<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PCNA Antibody [NB100-456] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- PCNA used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-NB100-456-img0044.jpg)
![Flow Cytometry: PCNA Antibody [NB100-456] - Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X106 cells were stained with 0.03 ug anti-PCNA . Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody], and DNA stained with PI.](http://images.novusbio.com/fullsize/PCNA-Antibody-Flow-Cytometry-NB100-456-img0004.jpg)
![Western Blot: PCNA Antibody [NB100-456] - A431 whole cell lysates](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0041.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Analysis using the Biotin conjugate of NB100-456. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0043.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PCNA Antibody [NB100-456] - Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 ug for IP; 30 ug for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody NB100-456 used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using another a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). immunoprecipitatesd PCNA was blotted using NB100-456 (1 ug/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunoprecipitation-NB100-456-img0047.jpg)
![Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Western-Blot-NBP2-32972-img0008.jpg "Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (G3.1) [NBP2-32972] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Immunocytochemistry-Immunofluorescence-NBP2-32972-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffin-embedded human Breast Carcinome stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Immunohistochemistry-Paraffin-NBP2-32972-img0004.jpg)
![Flow Cytometry: HSP27 Antibody (G3.1) [NBP2-32972] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Flow-Cytometry-NBP2-32972-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffim-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Immunohistochemistry-Paraffin-NBP2-32972-img0003.jpg)
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from MG63, human testis and mouse testis was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Western-Blot-NBP2-24702-img0009.jpg "Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from MG63, human testis and mouse testis was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence."){kind=icon}
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Formalin-fixed paraffin-embedded tissue sections of human liver were probed for RANK/TNFRSF11A mRNA (ACD RNAScope Probe, catalog # 412281; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # NBP2-24702) at 5 ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to plasma membrane.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Dual-RNAscope-ISH-IHC-NBP2-24702-img0011.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from Raw 264.7 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Western-Blot-NBP2-24702-img0010.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Analysis of antibody on (a) untreated BALB/c mouse splenocytes and (b) BALB/c mouse splenocytes stimulated with 5 ug/ml ConA for 72 hours. Cells were stained with 2 ug of antibody per 1x10^6 cells. Green represents isotype control; red represents antibody. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Flow-Cytometry-NBP2-24702-img0014.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Detection of RANK in RAW cells. Lane 1. detection of RANK in RAW, a mouse cell line. Lane 2 & 3. IP/analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or this antibody. RANK was detected by western blot using this antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Western-Blot-NBP2-24702-img0001.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Analysis on RAW cells using 5 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control; purple represents antibody. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Flow-Cytometry-NBP2-24702-img0013.jpg){kind=icon}
![Western Blot: Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - Total protein from human HeLa, Jurkat, and mouse MEF and Neuro2A cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Mre11 in block buffer and detected with an anti-Armenian Hamster HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Mre11-Antibody-15B8-1E7-6-Western-Blot-NBP2-59677-img0001.jpg "Western Blot: Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - Total protein from human HeLa, Jurkat, and mouse MEF and Neuro2A cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Mre11 in block buffer and detected with an anti-Armenian Hamster HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Mre11 (15B8.1E7.6) at 2 ug/ml overnight at 4C and detected with an anti-Armenian hamster IgG Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Mre11-Antibody-15B8-1E7-6-Immunocytochemistry-Immunofluorescence-NBP2-59677-img0003.jpg)
![Immunohistochemistry-Paraffin: Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse prostate tissue section using 1:500 dilution of Mre11 antibody (clone 15B8.1E7.6). The signal was developed using HRP-DAB indirect detection method and the sections were counterstained using hematoxylin staining. This Mre11 antibody generated a strong nuclear with mild to moderate cytoplasmic staining in the glandular epithelial cells.](http://images.novusbio.com/fullsize/Mre11-Antibody-15B8-1E7-6-Immunohistochemistry-Paraffin-NBP2-59677-img0005.jpg)
![Flow Cytometry: Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - An intracellular stain was performed on A431 cells with Mre11 [15B8.1E7.6] Antibody NBP2-59677B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/Mre11-Antibody-15B8-1E7-6-Flow-Cytometry-NBP2-59677-img0006.jpg)
![Immunohistochemistry-Paraffin: Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse prostate using 1:500 dilution of Mre11 antibody (clone 15B8.1E7.6). The signal was developed using HRP-DAB indirect detection method and the sections were counterstained using hematoxylin staining. This Mre11 antibody generated a strong nuclear positivity with considerable mild to moderate cytoplasmic staining in the glandular epithelial cells. Select cells depicted MRE11 foci which reflects towards the presence of DNA damage in those cells.](http://images.novusbio.com/fullsize/Mre11-Antibody-15B8-1E7-6-Immunohistochemistry-Paraffin-NBP2-59677-img0004.jpg)
![Flow (Intracellular): Mre11 Antibody (15B8.1E7.6) [NBP2-59677] - An intracellular stain was performed on HeLa Cells with Mre11 (15B8.1E7.6) antibody NBP2-59677 (blue). Unstained cells are shown in orange. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by armenian hamster IgG Alexa Flour 488-conjugated secondary.](http://images.novusbio.com/fullsize/Mre11-Antibody-15B8-1E7-6-Flow-Intracellular-NBP2-59677-img0002.jpg)
![Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Western-Blot-NBP2-37577-img0003.jpg "Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: AIF Antibody (4E7) [NBP2-37577] - Analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunocytochemistry-Immunofluorescence-NBP2-37577-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human lung cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0009.jpg)
![Flow Cytometry: AIF Antibody (4E7) [NBP2-37577] - Analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Flow-Cytometry-NBP2-37577-img0005.jpg)
![Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mAb against human AIF (AA: 1-261) recombinant protein. (Expected MW is 35.6 kDa)](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Western-Blot-NBP2-37577-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human breast cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0006.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human cervical cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human rectum cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0008.jpg)
![ELISA: AIF Antibody (4E7) [NBP2-37577] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-ELISA-NBP2-37577-img0001.jpg)