| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47782-0.1mg | |||
| IHC-Prediluted | NBP2-48352 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87249-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82537-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87137-25ul | |||
![Western Blot: Claspin Antibody [NB100-246] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Claspin antibody NB100-246 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Claspin-Antibody-Western-Blot-NB100-246-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Claspin Antibody [NB100-246] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Claspin antibody NB100-246 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Claspin Antibody [NB100-246] - Whole cell lysate (20 ug) from HeLa cells. Antibody used at 1 or 0.5 ug/ml with (+) or without (-) blocking peptide.](http://images.novusbio.com/fullsize/Claspin-Antibody-Western-Blot-NB100-246-img0002.jpg)
![Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-271-img0004.jpg "Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ATM Antibody [NB100-271] - Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat ATM antibody [NB100-271] used for IP at 3 ug per reaction. ATM was also immunoprecipitated by another goat anti-ATM antibody from Company B. For blotting immunoprecipitated ATM, ATM antibody [NB100-271] was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATM-Antibody-Immunoprecipitation-NB100-271-img0005.jpg)
![Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0200.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Whole Cell lysate (50 ug for WB, 500 ug for IP) from HeLa cells. Affinity purified goat anti-Chk1 used as indicated. Immunoprecipitated material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0003.jpg)
![Western Blot: CDC25A Antibody [NB100-213] - Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Western-Blot-NB100-213-img0009.jpg "Western Blot: CDC25A Antibody [NB100-213] - Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Western Blot: CDC25A Antibody [NB100-213] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-CDC25a antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Western-Blot-NB100-213-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CDC25A Antibody [NB100-213] - Detection of Human CDC25a by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Western-Blot-NB100-213-img0008.jpg)
![Western Blot: Rabbit Polyclonal CDC25A Antibody [NB100-213] - Whole cell lysates from MDA-MB-231, SUM159, MCF-7 and HEK293T cells were loaded with 50 ug/lane. 10% SDS-PAGE. CDC25A Antibody (NB100-213) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-213_rabbit-polyclonal-cdc25a-antibody-western-blot-3112024151137..png)
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (8F12) [NB100-500] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 1:500 dilution.](http://images.novusbio.com/fullsize/Chk2-Antibody-8F12-Immunocytochemistry-Immunofluorescence-NB100-500-img0003.jpg "Immunocytochemistry/Immunofluorescence: Chk2 Antibody (8F12) [NB100-500] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 1:500 dilution.")
![Western Blot: Chk2 Antibody (8F12) [NB100-500] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] (NB100-500) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nb100-500_mouse-monoclonal-chk2-antibody-8f12-91020242014681.jpg)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (SPM306) [NBP2-44526] - Human Colon Carcinoma stained with p21 Monoclonal Antibody (SPM306).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-SPM306-Immunohistochemistry-Paraffin-NBP2-44526-img0001.jpg "Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (SPM306) [NBP2-44526] - Human Colon Carcinoma stained with p21 Monoclonal Antibody (SPM306).")
![Western Blot: p21/CIP1/CDKN1A Antibody (SPM306) [NBP2-44526] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (SPM306).](http://images.novusbio.com/fullsize/nbp2-44526_mouse-monoclonal-p21-cip1-cdkn1a-antibody-spm306-17720241044622.jpg)
![Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.Lane 1: RFC4 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Western-Blot-H00005984-M01-img0011.jpg "Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.Lane 1: RFC4 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of monoclonal antibody to RFC4 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Immunohistochemistry-Paraffin-H00005984-M01-img0007.jpg)
![Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 over-expressed 293 cell line, cotransfected with RFC4 Validated Chimera RNAi ( Cat # H00005984-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RFC4 monoclonal antibody (M01), clone 1C12 (Cat # H00005984-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Western-Blot-H00005984-M01-img0008.jpg)
![Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - RFC4 monoclonal antibody (M01), clone 1C12 Analysis of RFC4 expression in K-562.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Western-Blot-H00005984-M01-img0010.jpg)
![Sandwich ELISA: RFC4 Antibody (1C12) [H00005984-M01] - Detection limit for recombinant GST tagged RFC4 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Sandwich-ELISA-H00005984-M01-img0009.jpg)
![Western Blot: RFC4 Antibody [NBP2-49283] - Analysis using Anti-RFC4 antibody NBP2-49283 (A) shows similar pattern to independent antibody NBP2-48927 (B).](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-49283-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RFC4 Antibody [NBP2-49283] - Analysis using Anti-RFC4 antibody NBP2-49283 (A) shows similar pattern to independent antibody NBP2-48927 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-49283] - Staining in human testis and kidney tissues using anti-RFC4 antibody. Corresponding RFC4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49283-img0005.jpg)
![Immunohistochemistry: RFC4 Antibody [NBP2-49283] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-NBP2-49283-img0001.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-49283] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49283-img0003.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-49283] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49283-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PPM1D Antibody [NBP1-87249] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87249-img0007.jpg "Immunocytochemistry/Immunofluorescence: PPM1D Antibody [NBP1-87249] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunohistochemistry-Paraffin-NBP1-87249-img0011.jpg)
![Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human liver shows moderate to strong nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunohistochemistry-Paraffin-NBP1-87249-img0008.jpg)
![Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunohistochemistry-Paraffin-NBP1-87249-img0009.jpg)
![Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in white matter.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunohistochemistry-Paraffin-NBP1-87249-img0010.jpg)
![Western Blot: TUSC4 Antibody [NBP1-82537] - Analysis in control (vector only transfected HEK293T lysate) and NPRL2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/TUSC4-Antibody-Western-Blot-NBP1-82537-img0009.jpg "Western Blot: TUSC4 Antibody [NBP1-82537] - Analysis in control (vector only transfected HEK293T lysate) and NPRL2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: TUSC4 Antibody [NBP1-82537] - Staining of human cell line PC-3 shows localization to cytosol & microtubules. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TUSC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82537-img0008.jpg)
![Immunohistochemistry-Paraffin: TUSC4 Antibody [NBP1-82537] - Staining of human pancreas shows strong cytoplasmic and/or nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/TUSC4-Antibody-Immunohistochemistry-Paraffin-NBP1-82537-img0007.jpg)
![Western Blot: RFC5 Antibody [NBP1-87137] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/RFC5-Antibody-Western-Blot-NBP1-87137-img0006.jpg "Western Blot: RFC5 Antibody [NBP1-87137] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: RFC5 Antibody [NBP1-87137] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RFC5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87137-img0008.jpg)
![Immunohistochemistry-Paraffin: RFC5 Antibody [NBP1-87137] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/RFC5-Antibody-Immunohistochemistry-Paraffin-NBP1-87137-img0002.jpg)
![Western Blot: RINT1 Antibody [NBP1-82312] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/RINT1-Antibody-Western-Blot-NBP1-82312-img0002.jpg "Western Blot: RINT1 Antibody [NBP1-82312] - Analysis in human cell line U-251 MG.")
![Immunohistochemistry-Paraffin: RINT1 Antibody [NBP1-82312] - Staining of human Fallopian tube shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/RINT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82312-img0005.jpg)
![Immunohistochemistry-Paraffin: RINT1 Antibody [NBP1-82312] - Staining of human fallopian tube shows strong membranous positivity in ciliated glandular cells.](http://images.novusbio.com/fullsize/RINT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82312-img0001.jpg)
![Immunohistochemistry-Paraffin: RINT1 Antibody [NBP1-82312] - Staining of human bronchus shows strong positivity in apical membrane in respiratory epithelial cells.](http://images.novusbio.com/fullsize/RINT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82312-img0003.jpg)
![Immunohistochemistry-Paraffin: RINT1 Antibody [NBP1-82312] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/RINT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82312-img0004.jpg)