| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44973 | |||
| Azide and BSA Free | NBP2-34621-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24702SS | |||
| Azide and BSA Free | NBP2-33256 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86912-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73253 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71662SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75477SS | |||
![Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Western-Blot-NBP2-32972-img0008.jpg "Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (G3.1) [NBP2-32972] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Immunocytochemistry-Immunofluorescence-NBP2-32972-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffin-embedded human Breast Carcinome stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Immunohistochemistry-Paraffin-NBP2-32972-img0004.jpg)
![Flow Cytometry: HSP27 Antibody (G3.1) [NBP2-32972] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Flow-Cytometry-NBP2-32972-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffim-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Immunohistochemistry-Paraffin-NBP2-32972-img0003.jpg)
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from MG63, human testis and mouse testis was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Western-Blot-NBP2-24702-img0009.jpg "Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from MG63, human testis and mouse testis was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence."){kind=icon}
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Formalin-fixed paraffin-embedded tissue sections of human liver were probed for RANK/TNFRSF11A mRNA (ACD RNAScope Probe, catalog # 412281; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # NBP2-24702) at 5 ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to plasma membrane.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Dual-RNAscope-ISH-IHC-NBP2-24702-img0011.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from Raw 264.7 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Western-Blot-NBP2-24702-img0010.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Analysis of antibody on (a) untreated BALB/c mouse splenocytes and (b) BALB/c mouse splenocytes stimulated with 5 ug/ml ConA for 72 hours. Cells were stained with 2 ug of antibody per 1x10^6 cells. Green represents isotype control; red represents antibody. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Flow-Cytometry-NBP2-24702-img0014.jpg){kind=icon}
![Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Detection of RANK in RAW cells. Lane 1. detection of RANK in RAW, a mouse cell line. Lane 2 & 3. IP/analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or this antibody. RANK was detected by western blot using this antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Western-Blot-NBP2-24702-img0001.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Analysis on RAW cells using 5 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control; purple represents antibody. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/RANK-TNFRSF11A-Antibody-64C1385-1-Flow-Cytometry-NBP2-24702-img0013.jpg){kind=icon}
![Immunocytochemistry/Immunofluorescence: TRAP alpha Antibody [NBP1-86912] - Staining of human cell line U-251 MG shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86912-img0007.jpg "Immunocytochemistry/Immunofluorescence: TRAP alpha Antibody [NBP1-86912] - Staining of human cell line U-251 MG shows localization to endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TRAP alpha Antibody [NBP1-86912] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/antibody/nbp1-86912_rabbit-polyclonal-trap-alpha-antibody-immunohistochemistry-paraffin-194202485445..jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody [NBP1-86912] -Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-86912_rabbit-polyclonal-trap-alpha-antibody-immunohistochemistry-paraffin-194202485535..jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody [NBP1-86912] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/antibody/nbp1-86912_rabbit-polyclonal-trap-alpha-antibody-immunohistochemistry-paraffin-19420248570..jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody [NBP1-86912] -Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-86912_rabbit-polyclonal-trap-alpha-antibody-immunohistochemistry-paraffin-194202485812..jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TRAP alpha Antibody [NBP1-86912] - Analysis using Anti-SSR1 antibody NBP1-86912 (A) shows similar pattern to independent antibody NBP1-87815 (B).](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-Western-Blot-NBP1-86912-img0010.jpg)
![Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Western-Blot-NBP2-37577-img0003.jpg "Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: AIF Antibody (4E7) [NBP2-37577] - Analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunocytochemistry-Immunofluorescence-NBP2-37577-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human lung cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0009.jpg)
![Flow Cytometry: AIF Antibody (4E7) [NBP2-37577] - Analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Flow-Cytometry-NBP2-37577-img0005.jpg)
![Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mAb against human AIF (AA: 1-261) recombinant protein. (Expected MW is 35.6 kDa)](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Western-Blot-NBP2-37577-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human breast cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0006.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human cervical cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human rectum cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0008.jpg)
![ELISA: AIF Antibody (4E7) [NBP2-37577] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-ELISA-NBP2-37577-img0001.jpg)
![Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of PTN expression in transfected 293T cell line by PTN monoclonal antibody (M01), clone 5C3.Lane 1: PTN transfected lysate(18.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Pleiotrophin-PTN-Antibody-5C3-Western-Blot-H00005764-M01-img0008.jpg "Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of PTN expression in transfected 293T cell line by PTN monoclonal antibody (M01), clone 5C3.Lane 1: PTN transfected lysate(18.9 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of monoclonal antibody to PTN on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Pleiotrophin-PTN-Antibody-5C3-Immunohistochemistry-Paraffin-H00005764-M01-img0006.jpg)
![Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - PTN monoclonal antibody (M01), clone 5C3 Analysis of PTN expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Pleiotrophin-PTN-Antibody-5C3-Western-Blot-H00005764-M01-img0007.jpg)
![Western Blot: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 monoclonal antibody (M01), clone 1E1. Analysis of RRM2 expression in HepG2.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Western-Blot-H00006241-M01-img0012.jpg "Western Blot: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 monoclonal antibody (M01), clone 1E1. Analysis of RRM2 expression in HepG2.")
![Immunocytochemistry/Immunofluorescence: RRM2 Antibody (1E1) [H00006241-M01] - Analysis of monoclonal antibody to RRM2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Immunocytochemistry-Immunofluorescence-H00006241-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: RRM2 Antibody (1E1) [H00006241-M01] - Analysis of monoclonal antibody to RRM2 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Immunohistochemistry-Paraffin-H00006241-M01-img0009.jpg)
![Western Blot: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 expression in HeLa NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Western-Blot-H00006241-M01-img0002.jpg)
![Immunoprecipitation: RRM2 Antibody (1E1) [H00006241-M01] - Analysis of RRM2 transfected lysate using anti-RRM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RRM2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Immunoprecipitation-H00006241-M01-img0010.jpg)
![ELISA: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 Antibody (1E1) [H00006241-M01] - Detection limit for recombinant GST tagged RRM2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-ELISA-H00006241-M01-img0013.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) and Western blotting (WB). Level of expression by WB and RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB and spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Knockdown-Validated-H00005997-M01-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) and Western blotting (WB). Level of expression by WB and RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB and spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Western-Blot-H00005997-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: RGS2 Antibody (4C4) [H00005997-M01] - Analysis of monoclonal antibody to RGS2 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Immunohistochemistry-Paraffin-H00005997-M01-img0005.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - RGS2 monoclonal antibody (M01), clone 4C4 Analysis of RGS2 expression in MCF-7.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Western-Blot-H00005997-M01-img0007.jpg)
![ELISA: RGS2 Antibody (4C4) [H00005997-M01] - Detection limit for recombinant GST tagged RGS2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-ELISA-H00005997-M01-img0008.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected & cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005997-m01_mouse-monoclonal-rgs2-antibody-4c4-310202415394536.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) & Western blotting (WB). A. Detection by RPPA of VCL & ERK2 in serial dilution of HEK293 cells transfected with siRNA targeting VCL (siVCL) or non-silencing siRNA control (siCTR). B. Linear regression was performed for ERK2 & VCL protein & Pearson correlation between dilution & the signal intensity was calculated for each condition of Figure 3A, with all p-values lower than 0.001. Signal intensities are expressed as mean of the replicates. C. Level of expression by WB & RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB & spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected & cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005997-m01_mouse-monoclonal-rgs2-antibody-4c4-310202415482256.jpg)
![Western Blot: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Patched 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Patched 1.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Western-Blot-NBP1-47945-img0023.jpg "Western Blot: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Patched 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Patched 1.")
![Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody (OTI5C7) [NBP1-47945] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Patched 1.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunocytochemistry-NBP1-47945-img0013.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Human endometrium tissue.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0024.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0014.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0015.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0016.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0017.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0018.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0019.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Patched 1 Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0020.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Human Kidney tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0021.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Human pancreas tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0022.jpg)
![Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched 1-PTCH Antibody - BSA Free-Western Blot-NB200-118-img0004.jpg "Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Evaluation of IGF-1, IGF1R and HH pathway expression in fibroblasts and oral squamous carcinoma cells. IGF1 mRNA expression in SCC-4 and MF1 cells, evaluated by RT-qPCR. (C) Evaluation of HH pathway component expression in MF1 by immunocytochemistry. Patched 1/PTCH Antibody (NB200-118); Sonic Hedgehog/Shh Antibody (NBP2-22126). Magnification: 400x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB200-118-img0003.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Increased Ihh mRNA and protein levels and activity in Smad2 and Smad3 mutant growth plates.All images are P0 proximal tibias. Immunostaining for Ptch1, showing increased protein levels throughout the growth plate in mutants, particularly in the resting zone. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006352) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Immunohistochemistry-NB200-118-img0002.jpg)
![Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Analysis of Human PTCH1 in Fetal brain Lysate with anti huPTCH1 at a 1:500 dilution.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Western-Blot-NB200-118-img0001.jpg)
![Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-13D10-Immunoprecipitation-NBP1-41201-img0017.jpg "Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [13D10] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-41201_mouse-monoclonal-rbbp4-rbap48-antibody-13d10-western-blot-196202494114..jpg)
![RbAp48 antibody [13D10] detects RbAp48 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] (NBP1-41201) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-41201_mouse-monoclonal-rbbp4-rbap48-antibody-13d10-immunocytochemistry-immunofluorescence-2611202518283514.jpg)
![Western Blot: Patched 1/PTCH Antibody [NBP1-71662] - Analysis of Patched 1 in human skeletal muscle protein.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Western-Blot-NBP1-71662-img0006.jpg "Western Blot: Patched 1/PTCH Antibody [NBP1-71662] - Analysis of Patched 1 in human skeletal muscle protein.")
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NBP1-71662] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse lung using 1:50 dilution of PTCH antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane-cytoplasmic staining in bronchiolar and alveolar epithelial cells with stronger signal towards the membranes of the cells.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Immunohistochemistry-Paraffin-NBP1-71662-img0005.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NBP1-71662] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse lung using 1:50 dilution of PTCH antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane-cytoplasmic staining in bronchiolar and alveolar epithelial cells with stronger signal towards the membranes of the cells.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Immunohistochemistry-Paraffin-NBP1-71662-img0004.jpg)
![Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP1-75477-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75477-img0006.jpg)
![Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-NBP1-75477-img0007.jpg)
![Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscle lysate.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0008.jpg)